Incidental Mutation 'R6605:Ifit2'
| ID |
525645 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Ifit2
|
| Ensembl Gene |
ENSMUSG00000045932 |
| Gene Name |
interferon-induced protein with tetratricopeptide repeats 2 |
| Synonyms |
Ifi54 |
| MMRRC Submission |
044728-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.062)
|
| Stock # |
R6605 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
19 |
| Chromosomal Location |
34528094-34553819 bp(+) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
C to T
at 34550897 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Stop codon
at position 146
(R146*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000099890
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000102826]
[ENSMUST00000149829]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably null
Transcript: ENSMUST00000102826
AA Change: R146*
|
| SMART Domains |
Protein: ENSMUSP00000099890
Gene: ENSMUSG00000045932
AA Change: R146*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:TPR_2
|
95 |
127 |
4e-4 |
PFAM |
|
Pfam:TPR_8
|
95 |
127 |
3.8e-4 |
PFAM |
|
Blast:TPR
|
138 |
171 |
7e-11 |
BLAST |
|
Blast:TPR
|
172 |
208 |
2e-9 |
BLAST |
|
low complexity region
|
211 |
222 |
N/A |
INTRINSIC |
|
Pfam:TPR_19
|
225 |
286 |
4e-8 |
PFAM |
|
low complexity region
|
396 |
406 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000149829
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.5%
- 10x: 97.9%
- 20x: 93.8%
|
| Validation Efficiency |
92% (35/38) |
| MGI Phenotype |
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susuceptibility to VSV infection with increased lethality and brain viral titer. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 39 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcc6 |
T |
A |
7: 45,630,481 (GRCm39) |
I1260F |
probably damaging |
Het |
| Acat2 |
A |
G |
17: 13,162,774 (GRCm39) |
V358A |
probably benign |
Het |
| Adgrv1 |
G |
A |
13: 81,636,081 (GRCm39) |
A3476V |
possibly damaging |
Het |
| Akap13 |
A |
G |
7: 75,229,516 (GRCm39) |
N150D |
probably damaging |
Het |
| Asb2 |
G |
T |
12: 103,311,943 (GRCm39) |
Q60K |
probably benign |
Het |
| Asxl3 |
A |
T |
18: 22,650,134 (GRCm39) |
R708* |
probably null |
Het |
| Cd79b |
C |
T |
11: 106,203,539 (GRCm39) |
G116D |
probably damaging |
Het |
| Copg2 |
A |
G |
6: 30,835,757 (GRCm39) |
F218S |
probably benign |
Het |
| Defa5 |
A |
C |
8: 21,787,604 (GRCm39) |
E50D |
possibly damaging |
Het |
| Efcab3 |
T |
A |
11: 104,890,107 (GRCm39) |
|
probably null |
Het |
| Eva1c |
T |
A |
16: 90,663,236 (GRCm39) |
V91D |
probably damaging |
Het |
| Fosb |
T |
C |
7: 19,043,283 (GRCm39) |
Y25C |
probably damaging |
Het |
| Gm5141 |
G |
A |
13: 62,922,201 (GRCm39) |
H323Y |
probably damaging |
Het |
| Gpr19 |
T |
C |
6: 134,847,398 (GRCm39) |
N58S |
probably benign |
Het |
| Gpr26 |
G |
A |
7: 131,585,893 (GRCm39) |
A288T |
possibly damaging |
Het |
| Gucy2g |
T |
A |
19: 55,229,460 (GRCm39) |
Q70L |
probably damaging |
Het |
| Lgr5 |
A |
C |
10: 115,293,772 (GRCm39) |
N408K |
possibly damaging |
Het |
| Lrp1 |
G |
A |
10: 127,396,005 (GRCm39) |
H2422Y |
probably damaging |
Het |
| Mief1 |
T |
A |
15: 80,132,692 (GRCm39) |
Y191* |
probably null |
Het |
| Npr3 |
C |
G |
15: 11,905,518 (GRCm39) |
A70P |
probably damaging |
Het |
| Or2y1b |
A |
T |
11: 49,208,541 (GRCm39) |
H56L |
probably damaging |
Het |
| Pcnt |
A |
G |
10: 76,265,032 (GRCm39) |
|
probably null |
Het |
| Pnlip |
A |
T |
19: 58,660,174 (GRCm39) |
D29V |
probably benign |
Het |
| Pnpt1 |
T |
A |
11: 29,088,567 (GRCm39) |
F277I |
possibly damaging |
Het |
| Pou2f2 |
A |
C |
7: 24,793,006 (GRCm39) |
V441G |
probably damaging |
Het |
| Prdm4 |
A |
G |
10: 85,740,002 (GRCm39) |
V459A |
probably benign |
Het |
| Ptprs |
A |
T |
17: 56,729,195 (GRCm39) |
V855E |
probably damaging |
Het |
| Rflna |
A |
G |
5: 125,088,352 (GRCm39) |
T100A |
probably benign |
Het |
| Rnd2 |
C |
T |
11: 101,359,825 (GRCm39) |
L57F |
probably damaging |
Het |
| Spata31h1 |
A |
T |
10: 82,131,871 (GRCm39) |
S380T |
probably benign |
Het |
| Syn3 |
A |
G |
10: 85,893,428 (GRCm39) |
S472P |
unknown |
Het |
| Taar8a |
A |
T |
10: 23,952,674 (GRCm39) |
I93F |
possibly damaging |
Het |
| Taok2 |
T |
A |
7: 126,477,930 (GRCm39) |
D207V |
probably damaging |
Het |
| Tpst2 |
A |
T |
5: 112,424,600 (GRCm39) |
|
probably benign |
Homo |
| Trdv2-1 |
A |
G |
14: 54,183,999 (GRCm39) |
N77S |
possibly damaging |
Het |
| Wdr17 |
A |
C |
8: 55,134,559 (GRCm39) |
V18G |
probably benign |
Het |
| Zbtb17 |
T |
C |
4: 141,192,261 (GRCm39) |
V402A |
probably damaging |
Het |
| Zfp772 |
T |
C |
7: 7,208,547 (GRCm39) |
E99G |
possibly damaging |
Het |
| Zw10 |
A |
G |
9: 48,980,926 (GRCm39) |
D442G |
probably benign |
Het |
|
| Other mutations in Ifit2 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01099:Ifit2
|
APN |
19 |
34,550,702 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02261:Ifit2
|
APN |
19 |
34,551,624 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02375:Ifit2
|
APN |
19 |
34,551,737 (GRCm39) |
missense |
probably benign |
0.01 |
|
Pushup
|
UTSW |
19 |
34,551,445 (GRCm39) |
missense |
probably benign |
0.38 |
|
R0017:Ifit2
|
UTSW |
19 |
34,550,973 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0017:Ifit2
|
UTSW |
19 |
34,550,973 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0682:Ifit2
|
UTSW |
19 |
34,551,012 (GRCm39) |
missense |
probably benign |
0.13 |
|
R0927:Ifit2
|
UTSW |
19 |
34,550,984 (GRCm39) |
missense |
probably benign |
0.03 |
|
R1462:Ifit2
|
UTSW |
19 |
34,550,586 (GRCm39) |
missense |
probably null |
0.12 |
|
R1462:Ifit2
|
UTSW |
19 |
34,550,586 (GRCm39) |
missense |
probably null |
0.12 |
|
R1526:Ifit2
|
UTSW |
19 |
34,550,602 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2084:Ifit2
|
UTSW |
19 |
34,550,750 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3971:Ifit2
|
UTSW |
19 |
34,551,441 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4008:Ifit2
|
UTSW |
19 |
34,551,445 (GRCm39) |
missense |
probably benign |
0.38 |
|
R4010:Ifit2
|
UTSW |
19 |
34,551,445 (GRCm39) |
missense |
probably benign |
0.38 |
|
R4011:Ifit2
|
UTSW |
19 |
34,551,445 (GRCm39) |
missense |
probably benign |
0.38 |
|
R4359:Ifit2
|
UTSW |
19 |
34,550,544 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R5179:Ifit2
|
UTSW |
19 |
34,550,976 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5240:Ifit2
|
UTSW |
19 |
34,551,796 (GRCm39) |
missense |
probably benign |
0.02 |
|
R5424:Ifit2
|
UTSW |
19 |
34,551,458 (GRCm39) |
missense |
probably benign |
0.19 |
|
R5528:Ifit2
|
UTSW |
19 |
34,550,937 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R7172:Ifit2
|
UTSW |
19 |
34,550,894 (GRCm39) |
missense |
probably benign |
0.24 |
|
R7424:Ifit2
|
UTSW |
19 |
34,550,598 (GRCm39) |
missense |
probably benign |
0.37 |
|
R8090:Ifit2
|
UTSW |
19 |
34,550,662 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
R8356:Ifit2
|
UTSW |
19 |
34,550,908 (GRCm39) |
nonsense |
probably null |
|
|
R8553:Ifit2
|
UTSW |
19 |
34,550,538 (GRCm39) |
missense |
probably benign |
0.00 |
|
X0023:Ifit2
|
UTSW |
19 |
34,551,650 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
X0064:Ifit2
|
UTSW |
19 |
34,551,323 (GRCm39) |
missense |
probably benign |
0.06 |
|
| Predicted Primers |
PCR Primer
(F):5'- CACTGCAATGCTTAGGGGAAG -3'
(R):5'- TGAATGGCTTGCTCCAGAGAG -3'
Sequencing Primer
(F):5'- TGAGGGCTTCATCCAGCAACAG -3'
(R):5'- TTGCTCCAGAGAGTCAATGC -3'
|
| Posted On |
2018-06-22 |
Incidental Mutation