Mutagenetix > Incidental Mutation

Incidental Mutation 'R6641:Nrsn2'

525769

Institutional Source

Beutler Lab

Gene Symbol

Nrsn2

Ensembl Gene

ENSMUSG00000059361

Gene Name

neurensin 2

Synonyms

LOC228777, Neurensin-2

MMRRC Submission

044762-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R6641 (G1)

Quality Score

202.009

Status

Validated

Chromosome

Chromosomal Location

152210675-152218558 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 152211830 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 67 (V67A)

Ref Sequence

ENSEMBL: ENSMUSP00000078260 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079278]

AlphaFold

Q5HZK2

Predicted Effect

probably benign
Transcript: ENSMUST00000079278
AA Change: V67A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000078260
Gene: ENSMUSG00000059361
AA Change: V67A

Domain	Start	End	E-Value	Type
Pfam:Neurensin	20	154	1.5e-37	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000089083

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128577

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.4%
20x: 91.6%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310034C09Rik	C	T	16: 88,555,974 (GRCm39)	P63S	unknown	Het
Aadac	T	C	3: 59,947,153 (GRCm39)	S284P	probably damaging	Het
Casp12	G	A	9: 5,354,612 (GRCm39)	C257Y	probably benign	Het
Chil6	T	A	3: 106,296,240 (GRCm39)	I361F	possibly damaging	Het
Cubn	A	C	2: 13,480,875 (GRCm39)	S327A	probably damaging	Het
Desi2	A	T	1: 178,071,943 (GRCm39)	E82D	possibly damaging	Het
Dym	T	A	18: 75,189,712 (GRCm39)	I100N	probably damaging	Het
Gm14418	G	T	2: 177,079,623 (GRCm39)	T124K	probably benign	Het
Gm28042	T	A	2: 119,870,164 (GRCm39)	I701N	probably damaging	Het
Gm3404	C	A	5: 146,464,518 (GRCm39)	A173D	probably damaging	Het
Hipk1	A	T	3: 103,660,721 (GRCm39)	L738Q	probably damaging	Het
Klk10	G	T	7: 43,434,324 (GRCm39)	D239Y	possibly damaging	Het
Kmt2a	T	C	9: 44,731,132 (GRCm39)		probably benign	Het
Lepr	T	A	4: 101,622,502 (GRCm39)	D427E	probably damaging	Het
Lrp5	G	A	19: 3,702,287 (GRCm39)	R177W	probably damaging	Het
Mtg2	A	G	2: 179,727,301 (GRCm39)	T318A	probably benign	Het
Myh7	A	G	14: 55,219,737 (GRCm39)	V1044A	probably benign	Het
Or2b11	T	C	11: 59,461,666 (GRCm39)	D300G	possibly damaging	Het
Pcdha8	A	G	18: 37,126,850 (GRCm39)	E444G	probably damaging	Het
Pdgfra	T	C	5: 75,322,762 (GRCm39)		probably benign	Het
Pik3c2a	T	C	7: 115,939,460 (GRCm39)		probably null	Het
Prpf40a	T	G	2: 53,031,638 (GRCm39)		probably benign	Het
Reln	A	C	5: 22,134,132 (GRCm39)	Y2599D	probably damaging	Het
Septin11	T	A	5: 93,287,411 (GRCm39)	I42N	probably damaging	Het
Slc22a15	G	A	3: 101,783,022 (GRCm39)	A216V	possibly damaging	Het
Slc33a1	T	A	3: 63,861,327 (GRCm39)	T292S	probably benign	Het
Slc5a11	A	G	7: 122,837,378 (GRCm39)	K56R	probably benign	Het
Slc7a13	G	A	4: 19,839,534 (GRCm39)	G379E	probably damaging	Het
Spata31g1	A	G	4: 42,971,245 (GRCm39)	I193V	possibly damaging	Het
Specc1l	A	G	10: 75,082,383 (GRCm39)	E593G	probably damaging	Het
Spef2	A	T	15: 9,626,059 (GRCm39)	M1169K	probably damaging	Het
Tmem161b	C	A	13: 84,370,537 (GRCm39)		probably benign	Het
Vipr1	A	G	9: 121,498,631 (GRCm39)	*460W	probably null	Het
Zbtb18	T	A	1: 177,275,609 (GRCm39)	L323Q	probably damaging	Het
Zfyve1	A	G	12: 83,641,270 (GRCm39)	S129P	probably benign	Het

Other mutations in Nrsn2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03303:Nrsn2	APN	2	152,216,131 (GRCm39)	missense	possibly damaging	0.65
R0403:Nrsn2	UTSW	2	152,211,710 (GRCm39)	missense	probably damaging	1.00
R0714:Nrsn2	UTSW	2	152,216,042 (GRCm39)	nonsense	probably null
R2570:Nrsn2	UTSW	2	152,211,741 (GRCm39)	missense	possibly damaging	0.55
R4840:Nrsn2	UTSW	2	152,211,552 (GRCm39)	missense	probably benign	0.42
R4856:Nrsn2	UTSW	2	152,211,531 (GRCm39)	missense	probably benign	0.09
R4886:Nrsn2	UTSW	2	152,211,531 (GRCm39)	missense	probably benign	0.09
R7796:Nrsn2	UTSW	2	152,218,471 (GRCm39)	start gained	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCAACCAGAGGCGATCAAG -3'
(R):5'- TTAGCTTTGGAAACCATTGCTAGGG -3'

Sequencing Primer
(F):5'- GCAGACAGCCAGCATGATC -3'
(R):5'- AGCTTTCAAGGCCATCGAG -3'

Posted On

2018-06-22