Incidental Mutation 'R6642:Il16'
ID 525845
Institutional Source Beutler Lab
Gene Symbol Il16
Ensembl Gene ENSMUSG00000001741
Gene Name interleukin 16
Synonyms
MMRRC Submission 044763-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.255) question?
Stock # R6642 (G1)
Quality Score 225.009
Status Validated
Chromosome 7
Chromosomal Location 83292033-83394934 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 83337335 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 127 (F127L)
Ref Sequence ENSEMBL: ENSMUSP00000118516 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000001792] [ENSMUST00000153560]
AlphaFold O54824
Predicted Effect probably benign
Transcript: ENSMUST00000001792
AA Change: F127L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000001792
Gene: ENSMUSG00000001741
AA Change: F127L

DomainStartEndE-ValueType
low complexity region 76 92 N/A INTRINSIC
low complexity region 99 115 N/A INTRINSIC
PDZ 222 300 6.5e-23 SMART
PDZ 361 438 3.89e-12 SMART
low complexity region 507 526 N/A INTRINSIC
low complexity region 556 577 N/A INTRINSIC
low complexity region 589 602 N/A INTRINSIC
low complexity region 647 680 N/A INTRINSIC
low complexity region 776 787 N/A INTRINSIC
low complexity region 825 839 N/A INTRINSIC
low complexity region 978 989 N/A INTRINSIC
PDZ 1115 1192 3.6e-16 SMART
low complexity region 1201 1216 N/A INTRINSIC
PDZ 1234 1310 4.11e-11 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000153560
AA Change: F127L

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000118516
Gene: ENSMUSG00000001741
AA Change: F127L

DomainStartEndE-ValueType
low complexity region 76 92 N/A INTRINSIC
low complexity region 99 115 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.8%
  • 20x: 93.3%
Validation Efficiency 100% (35/35)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
PHENOTYPE: Mice homozygous for a knock-out allele display a transient but consistent increase of thymidine incorporation in anti-CD3-stimulated CD4+ T cells, but fail to show a hyperproliferative T cell phenotype using BrdU labeling. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 34 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
6820408C15Rik T A 2: 152,282,790 (GRCm39) V215D probably damaging Het
Ablim1 A G 19: 57,119,284 (GRCm39) S267P probably benign Het
Adcy2 C T 13: 68,768,945 (GRCm39) C1061Y probably damaging Het
Aip C A 19: 4,165,149 (GRCm39) C240F probably damaging Het
Aldh1a2 A T 9: 71,160,268 (GRCm39) D98V probably damaging Het
Arhgef40 T C 14: 52,228,419 (GRCm39) probably benign Het
Cplx2 G T 13: 54,526,736 (GRCm39) R48L probably damaging Het
Ctrl C T 8: 106,659,451 (GRCm39) probably null Het
Dnhd1 A G 7: 105,353,006 (GRCm39) T2720A probably benign Het
Fzd1 A G 5: 4,805,696 (GRCm39) Y629H probably damaging Het
Gins1 T C 2: 150,770,038 (GRCm39) probably null Het
Gpr149 C T 3: 62,437,995 (GRCm39) A721T probably damaging Het
Helb A T 10: 119,920,835 (GRCm39) M1036K probably benign Het
Kctd20 A T 17: 29,180,640 (GRCm39) H138L probably damaging Het
Kctd9 T A 14: 67,962,122 (GRCm39) L55* probably null Het
Marf1 C T 16: 13,950,611 (GRCm39) R925H probably benign Het
Mbip A T 12: 56,389,191 (GRCm39) probably benign Het
Myo1c C T 11: 75,562,461 (GRCm39) P918S probably benign Het
Nod1 T G 6: 54,925,014 (GRCm39) D99A probably damaging Het
Olfm4 A G 14: 80,259,107 (GRCm39) K419E probably damaging Het
Or6c214 A T 10: 129,591,232 (GRCm39) L29Q probably damaging Het
Pik3r4 A G 9: 105,521,845 (GRCm39) D137G probably benign Het
Prdm4 T C 10: 85,743,682 (GRCm39) E191G probably benign Het
Rassf10 A T 7: 112,554,784 (GRCm39) T462S probably benign Het
Rundc3b A T 5: 8,629,071 (GRCm39) I110N probably damaging Het
Sgsm3 C T 15: 80,893,901 (GRCm39) R479C probably damaging Het
Tmc7 A T 7: 118,144,834 (GRCm39) Y575* probably null Het
Trim33 T C 3: 103,244,830 (GRCm39) L310S probably damaging Het
Trpm2 C T 10: 77,773,660 (GRCm39) R585Q probably benign Het
Ttn T C 2: 76,565,740 (GRCm39) E28204G probably damaging Het
Vmn1r233 A T 17: 21,214,002 (GRCm39) L316Q probably damaging Het
Vmn2r110 A T 17: 20,803,779 (GRCm39) N265K possibly damaging Het
Xylb A G 9: 119,196,559 (GRCm39) H114R probably damaging Het
Ywhaz T C 15: 36,791,166 (GRCm39) Y19C probably damaging Het
Other mutations in Il16
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00427:Il16 APN 7 83,301,666 (GRCm39) missense probably benign 0.02
IGL01743:Il16 APN 7 83,301,507 (GRCm39) missense probably benign 0.00
IGL01770:Il16 APN 7 83,322,234 (GRCm39) splice site probably benign
IGL02025:Il16 APN 7 83,302,056 (GRCm39) missense probably damaging 1.00
IGL02317:Il16 APN 7 83,316,097 (GRCm39) missense probably damaging 1.00
IGL02412:Il16 APN 7 83,301,899 (GRCm39) missense probably benign 0.03
IGL02550:Il16 APN 7 83,323,704 (GRCm39) missense possibly damaging 0.90
IGL02568:Il16 APN 7 83,310,484 (GRCm39) missense probably damaging 1.00
IGL02578:Il16 APN 7 83,327,194 (GRCm39) critical splice donor site probably null
IGL02815:Il16 APN 7 83,300,249 (GRCm39) missense probably damaging 0.98
IGL03157:Il16 APN 7 83,371,611 (GRCm39) missense probably damaging 1.00
IGL03161:Il16 APN 7 83,371,707 (GRCm39) missense probably damaging 1.00
IGL03188:Il16 APN 7 83,337,371 (GRCm39) missense probably benign 0.00
IGL03213:Il16 APN 7 83,295,708 (GRCm39) missense probably damaging 1.00
IGL03274:Il16 APN 7 83,310,442 (GRCm39) missense probably damaging 1.00
R0201:Il16 UTSW 7 83,371,516 (GRCm39) missense probably damaging 0.99
R0309:Il16 UTSW 7 83,371,762 (GRCm39) missense probably damaging 1.00
R0597:Il16 UTSW 7 83,327,183 (GRCm39) splice site probably benign
R0942:Il16 UTSW 7 83,312,349 (GRCm39) missense probably benign 0.01
R1018:Il16 UTSW 7 83,323,746 (GRCm39) missense probably damaging 1.00
R1434:Il16 UTSW 7 83,304,520 (GRCm39) missense probably benign
R1715:Il16 UTSW 7 83,297,936 (GRCm39) missense probably benign 0.01
R2179:Il16 UTSW 7 83,337,287 (GRCm39) splice site probably null
R2520:Il16 UTSW 7 83,301,202 (GRCm39) missense probably benign 0.03
R3425:Il16 UTSW 7 83,293,248 (GRCm39) missense probably damaging 1.00
R3761:Il16 UTSW 7 83,300,093 (GRCm39) missense possibly damaging 0.96
R3943:Il16 UTSW 7 83,301,223 (GRCm39) missense probably damaging 0.97
R4470:Il16 UTSW 7 83,300,046 (GRCm39) intron probably benign
R4530:Il16 UTSW 7 83,330,518 (GRCm39) intron probably benign
R4583:Il16 UTSW 7 83,332,107 (GRCm39) missense probably damaging 1.00
R4777:Il16 UTSW 7 83,300,104 (GRCm39) missense probably benign 0.14
R4874:Il16 UTSW 7 83,310,153 (GRCm39) missense possibly damaging 0.56
R4876:Il16 UTSW 7 83,322,302 (GRCm39) missense probably benign
R5677:Il16 UTSW 7 83,323,761 (GRCm39) missense probably damaging 1.00
R5686:Il16 UTSW 7 83,297,936 (GRCm39) missense probably benign 0.36
R5920:Il16 UTSW 7 83,301,552 (GRCm39) missense probably benign 0.03
R6115:Il16 UTSW 7 83,301,775 (GRCm39) nonsense probably null
R6459:Il16 UTSW 7 83,371,536 (GRCm39) missense probably damaging 1.00
R6459:Il16 UTSW 7 83,371,529 (GRCm39) missense probably damaging 1.00
R6601:Il16 UTSW 7 83,371,677 (GRCm39) missense probably damaging 1.00
R6616:Il16 UTSW 7 83,295,684 (GRCm39) missense probably benign 0.37
R6721:Il16 UTSW 7 83,312,270 (GRCm39) critical splice donor site probably null
R7009:Il16 UTSW 7 83,295,596 (GRCm39) missense probably benign
R7144:Il16 UTSW 7 83,295,659 (GRCm39) missense probably damaging 0.97
R7346:Il16 UTSW 7 83,293,249 (GRCm39) missense probably damaging 1.00
R7403:Il16 UTSW 7 83,319,343 (GRCm39) missense probably damaging 1.00
R7499:Il16 UTSW 7 83,323,702 (GRCm39) missense probably damaging 0.99
R7814:Il16 UTSW 7 83,319,348 (GRCm39) missense possibly damaging 0.46
R7941:Il16 UTSW 7 83,332,037 (GRCm39) missense probably damaging 0.98
R8098:Il16 UTSW 7 83,295,767 (GRCm39) missense probably damaging 1.00
R8317:Il16 UTSW 7 83,304,538 (GRCm39) missense probably benign
R8437:Il16 UTSW 7 83,301,351 (GRCm39) missense probably damaging 1.00
R9094:Il16 UTSW 7 83,301,559 (GRCm39) missense probably benign
R9267:Il16 UTSW 7 83,371,757 (GRCm39) missense probably benign 0.01
R9445:Il16 UTSW 7 83,337,380 (GRCm39) nonsense probably null
R9595:Il16 UTSW 7 83,322,273 (GRCm39) nonsense probably null
R9651:Il16 UTSW 7 83,332,064 (GRCm39) missense probably damaging 0.96
Z1176:Il16 UTSW 7 83,302,035 (GRCm39) missense probably benign 0.05
Predicted Primers PCR Primer
(F):5'- TAATCTCCCTCACAGCACTGG -3'
(R):5'- GGCAAATCAACAAGTATTTCTCTGG -3'

Sequencing Primer
(F):5'- AATCCATAGGTCTCCAGGTGATG -3'
(R):5'- AGTATTTCTCTGGTCCATGCCAACAG -3'
Posted On 2018-06-22