Mutagenetix > Incidental Mutation

Incidental Mutation 'R6645:Vmn2r6'

526055

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r6

Ensembl Gene

ENSMUSG00000090581

Gene Name

vomeronasal 2, receptor 6

Synonyms

EG620718, EG667069

MMRRC Submission

044766-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.241) question?

Stock #

R6645 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64537561-64565298 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 64556876 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 179 (V179E)

Ref Sequence

ENSEMBL: ENSMUSP00000135148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165012] [ENSMUST00000176481]

AlphaFold

H3BK29

Predicted Effect

probably damaging
Transcript: ENSMUST00000165012
AA Change: V90E

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000131831
Gene: ENSMUSG00000090581
AA Change: V90E

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	1.4e-72	PFAM
Pfam:Peripla_BP_6	58	244	1.2e-10	PFAM
Pfam:NCD3G	458	511	1.8e-17	PFAM
Pfam:7tm_3	542	779	3.9e-76	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000176481
AA Change: V179E

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000135148
Gene: ENSMUSG00000090581
AA Change: V179E

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	88	505	9.8e-77	PFAM
Pfam:Peripla_BP_6	142	331	3.4e-10	PFAM
Pfam:NCD3G	547	600	5.4e-17	PFAM
Pfam:7tm_3	633	867	3.9e-47	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 93.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Anp32e	T	A	3: 95,937,102 (GRCm38)	F95I	probably damaging	Het
Arap1	A	G	7: 101,408,111 (GRCm38)	K628R	possibly damaging	Het
Arid4b	A	T	13: 14,120,152 (GRCm38)	E6D	probably damaging	Het
Atxn10	G	T	15: 85,376,703 (GRCm38)		probably null	Het
Ccdc170	A	G	10: 4,560,974 (GRCm38)	I678V	possibly damaging	Het
Ccdc18	T	A	5: 108,138,930 (GRCm38)	V110D	probably benign	Het
Cep85l	C	T	10: 53,301,672 (GRCm38)	E322K	probably benign	Het
Cilp	A	T	9: 65,279,305 (GRCm38)	Y894F	possibly damaging	Het
Ddx4	A	G	13: 112,641,174 (GRCm38)	S62P	possibly damaging	Het
Dph7	T	C	2: 24,965,651 (GRCm38)	V154A	probably benign	Het
Ephb6	T	C	6: 41,617,272 (GRCm38)	S579P	probably benign	Het
Fam53a	G	T	5: 33,600,784 (GRCm38)	Q332K	probably benign	Het
Fancm	A	G	12: 65,106,100 (GRCm38)	D1110G	probably damaging	Het
Fh1	A	T	1: 175,614,876 (GRCm38)	V136E	possibly damaging	Het
Greb1	G	T	12: 16,698,579 (GRCm38)	H1132Q	probably benign	Het
Jph1	A	G	1: 17,091,761 (GRCm38)	S226P	probably damaging	Het
Kbtbd8	C	T	6: 95,126,749 (GRCm38)	R460*	probably null	Het
Lama5	T	A	2: 180,179,670 (GRCm38)	N3059Y	probably damaging	Het
Lipc	G	A	9: 70,803,748 (GRCm38)	T289I	probably damaging	Het
Lrrc2	T	C	9: 110,970,107 (GRCm38)	W241R	probably damaging	Het
Mfn2	T	A	4: 147,894,612 (GRCm38)	I88F	probably damaging	Het
Mms19	G	C	19: 41,955,191 (GRCm38)	N366K	probably benign	Het
Myo15a	A	G	11: 60,477,292 (GRCm38)	T293A	probably benign	Het
Ndfip2	T	A	14: 105,292,273 (GRCm38)	Y179N	probably damaging	Het
Notch4	A	G	17: 34,587,816 (GRCm38)	D1909G	probably benign	Het
Obscn	C	T	11: 59,085,262 (GRCm38)	S2013N	probably damaging	Het
Oca2	G	T	7: 56,314,774 (GRCm38)	A357S	probably benign	Het
Or8b43	T	C	9: 38,448,923 (GRCm38)	L17S	probably damaging	Het
Or8g35	T	A	9: 39,470,266 (GRCm38)	L153F	probably benign	Het
Pde7b	C	A	10: 20,610,566 (GRCm38)		probably null	Het
Ppef2	T	C	5: 92,230,461 (GRCm38)	N625S	probably benign	Het
Prom1	A	T	5: 44,047,514 (GRCm38)	L192Q	probably damaging	Het
Satb2	T	C	1: 56,797,007 (GRCm38)	I542V	possibly damaging	Het
Sgpp2	C	T	1: 78,360,162 (GRCm38)	T59M	probably damaging	Het
Skint6	T	C	4: 112,892,038 (GRCm38)	T782A	possibly damaging	Het
Slc13a4	G	T	6: 35,268,839 (GRCm38)	Q624K	probably benign	Het
Slc9a3	T	A	13: 74,164,172 (GRCm38)	H629Q	probably damaging	Het
Slitrk3	G	T	3: 73,049,861 (GRCm38)	A526E	probably benign	Het
Spata31e2	T	A	1: 26,683,117 (GRCm38)	D994V	probably benign	Het
Sptssa	T	C	12: 54,646,490 (GRCm38)	Y53C	probably damaging	Het
Srsf10	T	C	4: 135,863,563 (GRCm38)	S159P	possibly damaging	Het
Tbce	T	C	13: 14,005,229 (GRCm38)	T341A	probably benign	Het
Tdrd6	A	G	17: 43,624,532 (GRCm38)	L1875P	probably benign	Het
Tkt	G	A	14: 30,570,211 (GRCm38)	G425R	probably damaging	Het
Tmprss7	T	C	16: 45,690,963 (GRCm38)	I17M	possibly damaging	Het
Ttc21b	T	C	2: 66,236,377 (GRCm38)	S311G	probably benign	Het
Ubr5	A	G	15: 38,029,506 (GRCm38)	Y492H	probably damaging	Het
Ush2a	T	C	1: 188,523,331 (GRCm38)	I1535T	probably damaging	Het
Vmn2r17	A	T	5: 109,428,381 (GRCm38)	N373Y	probably damaging	Het
Vps13b	A	G	15: 35,910,305 (GRCm38)	E3405G	probably benign	Het
Wac	A	T	18: 7,973,523 (GRCm38)	Q212H	probably damaging	Het
Washc4	C	T	10: 83,572,195 (GRCm38)	R555*	probably null	Het
Zmat4	G	A	8: 23,797,401 (GRCm38)		probably null	Het

Other mutations in Vmn2r6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01547:Vmn2r6	APN	3	64,538,104 (GRCm38)	missense	probably damaging	1.00
IGL01968:Vmn2r6	APN	3	64,556,345 (GRCm38)	missense	possibly damaging	0.94
IGL02009:Vmn2r6	APN	3	64,537,902 (GRCm38)	missense	possibly damaging	0.61
IGL02039:Vmn2r6	APN	3	64,556,189 (GRCm38)	missense	probably damaging	1.00
IGL02652:Vmn2r6	APN	3	64,556,328 (GRCm38)	missense	probably benign	0.24
IGL02737:Vmn2r6	APN	3	64,556,490 (GRCm38)	missense	possibly damaging	0.55
IGL02808:Vmn2r6	APN	3	64,556,496 (GRCm38)	missense	probably damaging	1.00
IGL03066:Vmn2r6	APN	3	64,565,153 (GRCm38)	missense	probably damaging	0.99
IGL03331:Vmn2r6	APN	3	64,538,007 (GRCm38)	missense	probably damaging	1.00
BB010:Vmn2r6	UTSW	3	64,559,803 (GRCm38)	missense	probably benign	0.02
BB020:Vmn2r6	UTSW	3	64,559,803 (GRCm38)	missense	probably benign	0.02
R0010:Vmn2r6	UTSW	3	64,559,545 (GRCm38)	nonsense	probably null
R0206:Vmn2r6	UTSW	3	64,539,912 (GRCm38)	missense	probably benign
R0206:Vmn2r6	UTSW	3	64,539,912 (GRCm38)	missense	probably benign
R0208:Vmn2r6	UTSW	3	64,539,912 (GRCm38)	missense	probably benign
R0427:Vmn2r6	UTSW	3	64,559,587 (GRCm38)	missense	probably damaging	1.00
R0466:Vmn2r6	UTSW	3	64,556,302 (GRCm38)	missense	probably damaging	1.00
R1018:Vmn2r6	UTSW	3	64,556,840 (GRCm38)	missense	probably benign	0.00
R1104:Vmn2r6	UTSW	3	64,538,066 (GRCm38)	missense	possibly damaging	0.93
R1186:Vmn2r6	UTSW	3	64,565,067 (GRCm38)	missense	probably benign	0.01
R1245:Vmn2r6	UTSW	3	64,556,790 (GRCm38)	missense	possibly damaging	0.53
R1295:Vmn2r6	UTSW	3	64,538,273 (GRCm38)	missense	probably damaging	1.00
R1473:Vmn2r6	UTSW	3	64,538,158 (GRCm38)	nonsense	probably null
R1498:Vmn2r6	UTSW	3	64,556,469 (GRCm38)	missense	probably damaging	1.00
R1925:Vmn2r6	UTSW	3	64,556,277 (GRCm38)	missense	possibly damaging	0.87
R2044:Vmn2r6	UTSW	3	64,537,841 (GRCm38)	missense	probably damaging	0.96
R2069:Vmn2r6	UTSW	3	64,556,098 (GRCm38)	missense	possibly damaging	0.89
R2253:Vmn2r6	UTSW	3	64,559,718 (GRCm38)	missense	probably damaging	1.00
R2261:Vmn2r6	UTSW	3	64,556,669 (GRCm38)	missense	probably benign	0.24
R2262:Vmn2r6	UTSW	3	64,556,669 (GRCm38)	missense	probably benign	0.24
R2350:Vmn2r6	UTSW	3	64,556,352 (GRCm38)	missense	probably benign	0.01
R2680:Vmn2r6	UTSW	3	64,538,286 (GRCm38)	missense	possibly damaging	0.91
R2846:Vmn2r6	UTSW	3	64,556,790 (GRCm38)	missense	possibly damaging	0.53
R2860:Vmn2r6	UTSW	3	64,547,339 (GRCm38)	missense	probably benign	0.00
R2861:Vmn2r6	UTSW	3	64,547,339 (GRCm38)	missense	probably benign	0.00
R3766:Vmn2r6	UTSW	3	64,556,508 (GRCm38)	missense	probably benign	0.19
R3870:Vmn2r6	UTSW	3	64,556,621 (GRCm38)	missense	probably damaging	0.96
R4018:Vmn2r6	UTSW	3	64,556,472 (GRCm38)	missense	probably benign	0.05
R4024:Vmn2r6	UTSW	3	64,538,250 (GRCm38)	missense	possibly damaging	0.73
R4026:Vmn2r6	UTSW	3	64,538,250 (GRCm38)	missense	possibly damaging	0.73
R4227:Vmn2r6	UTSW	3	64,537,948 (GRCm38)	missense	probably damaging	0.99
R4526:Vmn2r6	UTSW	3	64,537,724 (GRCm38)	missense	probably benign	0.32
R4570:Vmn2r6	UTSW	3	64,559,647 (GRCm38)	missense	probably benign	0.31
R4894:Vmn2r6	UTSW	3	64,547,408 (GRCm38)	missense	probably benign
R4934:Vmn2r6	UTSW	3	64,556,345 (GRCm38)	missense	probably damaging	0.99
R5057:Vmn2r6	UTSW	3	64,537,786 (GRCm38)	missense	probably damaging	1.00
R5059:Vmn2r6	UTSW	3	64,537,623 (GRCm38)	missense	possibly damaging	0.89
R5148:Vmn2r6	UTSW	3	64,556,594 (GRCm38)	missense	probably damaging	0.99
R5155:Vmn2r6	UTSW	3	64,538,514 (GRCm38)	missense	probably benign	0.44
R5179:Vmn2r6	UTSW	3	64,537,990 (GRCm38)	missense	probably benign	0.00
R5256:Vmn2r6	UTSW	3	64,556,842 (GRCm38)	missense	probably benign	0.33
R5861:Vmn2r6	UTSW	3	64,556,033 (GRCm38)	missense	probably benign	0.00
R5950:Vmn2r6	UTSW	3	64,565,231 (GRCm38)	missense	probably benign	0.05
R6081:Vmn2r6	UTSW	3	64,556,532 (GRCm38)	missense	probably benign	0.25
R6173:Vmn2r6	UTSW	3	64,559,755 (GRCm38)	missense	probably damaging	1.00
R6190:Vmn2r6	UTSW	3	64,538,003 (GRCm38)	missense	probably benign	0.04
R6240:Vmn2r6	UTSW	3	64,556,805 (GRCm38)	missense	probably damaging	1.00
R6433:Vmn2r6	UTSW	3	64,547,380 (GRCm38)	nonsense	probably null
R6791:Vmn2r6	UTSW	3	64,538,159 (GRCm38)	missense	probably damaging	1.00
R7265:Vmn2r6	UTSW	3	64,556,774 (GRCm38)	missense	probably benign	0.00
R7503:Vmn2r6	UTSW	3	64,539,951 (GRCm38)	nonsense	probably null
R7562:Vmn2r6	UTSW	3	64,556,520 (GRCm38)	missense	probably benign	0.00
R7584:Vmn2r6	UTSW	3	64,565,262 (GRCm38)	missense	probably benign	0.07
R7611:Vmn2r6	UTSW	3	64,565,142 (GRCm38)	missense	probably damaging	0.98
R7759:Vmn2r6	UTSW	3	64,556,570 (GRCm38)	missense	probably damaging	1.00
R7834:Vmn2r6	UTSW	3	64,538,022 (GRCm38)	missense	probably damaging	1.00
R7933:Vmn2r6	UTSW	3	64,559,803 (GRCm38)	missense	probably benign	0.02
R7982:Vmn2r6	UTSW	3	64,559,820 (GRCm38)	missense	probably damaging	1.00
R8024:Vmn2r6	UTSW	3	64,559,824 (GRCm38)	missense	probably benign	0.40
R8074:Vmn2r6	UTSW	3	64,547,643 (GRCm38)	intron	probably benign
R8169:Vmn2r6	UTSW	3	64,539,889 (GRCm38)	missense	probably benign	0.01
R8337:Vmn2r6	UTSW	3	64,556,105 (GRCm38)	nonsense	probably null
R8736:Vmn2r6	UTSW	3	64,559,800 (GRCm38)	missense	probably damaging	1.00
R8962:Vmn2r6	UTSW	3	64,556,155 (GRCm38)	missense	probably damaging	1.00
R9139:Vmn2r6	UTSW	3	64,556,856 (GRCm38)	missense	probably benign	0.12
R9206:Vmn2r6	UTSW	3	64,559,611 (GRCm38)	missense	probably damaging	0.97
R9295:Vmn2r6	UTSW	3	64,556,063 (GRCm38)	missense	probably benign	0.00
R9332:Vmn2r6	UTSW	3	64,547,250 (GRCm38)	missense	probably benign	0.01
R9616:Vmn2r6	UTSW	3	64,538,303 (GRCm38)	missense	probably damaging	1.00
R9663:Vmn2r6	UTSW	3	64,556,128 (GRCm38)	missense	possibly damaging	0.90
R9685:Vmn2r6	UTSW	3	64,556,660 (GRCm38)	missense	probably benign	0.19
X0020:Vmn2r6	UTSW	3	64,538,450 (GRCm38)	missense	probably benign
X0066:Vmn2r6	UTSW	3	64,547,378 (GRCm38)	missense	probably damaging	1.00
Z1176:Vmn2r6	UTSW	3	64,556,325 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AACACAAAGGTTGGCCCTCTC -3'
(R):5'- TGTCAAAATTGTCCCCATCTACAC -3'

Sequencing Primer
(F):5'- AAAGGTTGGCCCTCTCCATTTC -3'
(R):5'- TTGTCCCCATCTACACCCAGAAAG -3'

Posted On

2018-06-22