Mutagenetix > Incidental Mutation

Incidental Mutation 'R6663:Zfp668'

526874

Institutional Source

Beutler Lab

Gene Symbol

Zfp668

Ensembl Gene

ENSMUSG00000049728

Gene Name

zinc finger protein 668

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R6663 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

127863047-127876828 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 127867769 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 212 (R212C)

Ref Sequence

ENSEMBL: ENSMUSP00000101870 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054415] [ENSMUST00000106261] [ENSMUST00000106262] [ENSMUST00000106263] [ENSMUST00000144721]

AlphaFold

Q8K2R5

Predicted Effect

probably damaging
Transcript: ENSMUST00000054415
AA Change: R212C

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000056105
Gene: ENSMUSG00000049728
AA Change: R212C

Domain	Start	End	E-Value	Type
ZnF_C2H2	22	44	7.67e-2	SMART
ZnF_C2H2	84	106	9.44e-2	SMART
ZnF_C2H2	112	134	3.63e-3	SMART
ZnF_C2H2	140	162	2.36e-2	SMART
ZnF_C2H2	168	190	1.98e-4	SMART
ZnF_C2H2	196	218	2.91e-2	SMART
ZnF_C2H2	224	246	1.26e-2	SMART
ZnF_C2H2	252	274	5.21e-4	SMART
ZnF_C2H2	280	302	2.57e-3	SMART
ZnF_C2H2	308	330	5.59e-4	SMART
ZnF_C2H2	336	358	1.79e-2	SMART
ZnF_C2H2	364	386	7.9e-4	SMART
ZnF_C2H2	392	414	5.5e-3	SMART
low complexity region	428	470	N/A	INTRINSIC
ZnF_C2H2	516	538	3.63e-3	SMART
ZnF_C2H2	544	566	1.3e-4	SMART
ZnF_C2H2	572	594	5.59e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106261
AA Change: R212C

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101868
Gene: ENSMUSG00000049728
AA Change: R212C

Domain	Start	End	E-Value	Type
ZnF_C2H2	22	44	7.67e-2	SMART
ZnF_C2H2	84	106	9.44e-2	SMART
ZnF_C2H2	112	134	3.63e-3	SMART
ZnF_C2H2	140	162	2.36e-2	SMART
ZnF_C2H2	168	190	1.98e-4	SMART
ZnF_C2H2	196	218	2.91e-2	SMART
ZnF_C2H2	224	246	1.26e-2	SMART
ZnF_C2H2	252	274	5.21e-4	SMART
ZnF_C2H2	280	302	2.57e-3	SMART
ZnF_C2H2	308	330	5.59e-4	SMART
ZnF_C2H2	336	358	1.79e-2	SMART
ZnF_C2H2	364	386	7.9e-4	SMART
ZnF_C2H2	392	414	5.5e-3	SMART
low complexity region	428	470	N/A	INTRINSIC
ZnF_C2H2	516	538	3.63e-3	SMART
ZnF_C2H2	544	566	1.3e-4	SMART
ZnF_C2H2	572	594	5.59e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106262
AA Change: R212C

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101869
Gene: ENSMUSG00000049728
AA Change: R212C

Domain	Start	End	E-Value	Type
ZnF_C2H2	22	44	7.67e-2	SMART
ZnF_C2H2	84	106	9.44e-2	SMART
ZnF_C2H2	112	134	3.63e-3	SMART
ZnF_C2H2	140	162	2.36e-2	SMART
ZnF_C2H2	168	190	1.98e-4	SMART
ZnF_C2H2	196	218	2.91e-2	SMART
ZnF_C2H2	224	246	1.26e-2	SMART
ZnF_C2H2	252	274	5.21e-4	SMART
ZnF_C2H2	280	302	2.57e-3	SMART
ZnF_C2H2	308	330	5.59e-4	SMART
ZnF_C2H2	336	358	1.79e-2	SMART
ZnF_C2H2	364	386	7.9e-4	SMART
ZnF_C2H2	392	414	5.5e-3	SMART
low complexity region	428	470	N/A	INTRINSIC
ZnF_C2H2	516	538	3.63e-3	SMART
ZnF_C2H2	544	566	1.3e-4	SMART
ZnF_C2H2	572	594	5.59e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000106263
AA Change: R212C

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101870
Gene: ENSMUSG00000049728
AA Change: R212C

Domain	Start	End	E-Value	Type
ZnF_C2H2	22	44	7.67e-2	SMART
ZnF_C2H2	84	106	9.44e-2	SMART
ZnF_C2H2	112	134	3.63e-3	SMART
ZnF_C2H2	140	162	2.36e-2	SMART
ZnF_C2H2	168	190	1.98e-4	SMART
ZnF_C2H2	196	218	2.91e-2	SMART
ZnF_C2H2	224	246	1.26e-2	SMART
ZnF_C2H2	252	274	5.21e-4	SMART
ZnF_C2H2	280	302	2.57e-3	SMART
ZnF_C2H2	308	330	5.59e-4	SMART
ZnF_C2H2	336	358	1.79e-2	SMART
ZnF_C2H2	364	386	7.9e-4	SMART
ZnF_C2H2	392	414	5.5e-3	SMART
low complexity region	428	470	N/A	INTRINSIC
ZnF_C2H2	516	538	3.63e-3	SMART
ZnF_C2H2	544	566	1.3e-4	SMART
ZnF_C2H2	572	594	5.59e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000144721

SMART Domains

Protein: ENSMUSP00000116730
Gene: ENSMUSG00000049728

Domain	Start	End	E-Value	Type
ZnF_C2H2	22	44	7.67e-2	SMART
ZnF_C2H2	84	106	9.44e-2	SMART

Meta Mutation Damage Score

0.2719

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.9%
20x: 94.0%

Validation Efficiency

100% (32/32)

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd17	A	G	5: 90,264,064	S1314P	probably damaging	Het
Btbd3	T	A	2: 138,279,083	I59K	probably benign	Het
Clcn2	A	G	16: 20,703,245	*865R	probably null	Het
Col22a1	T	C	15: 71,820,059	Q749R	unknown	Het
Cpsf2	T	C	12: 101,999,593	Y606H	probably damaging	Het
Csn1s1	T	C	5: 87,675,740	V154A	probably benign	Het
Cux1	T	A	5: 136,485,847	E23V	probably damaging	Het
Cyp2j9	C	A	4: 96,579,442	W269L	probably benign	Het
Dbf4	T	C	5: 8,403,184	M273V	probably benign	Het
Dnhd1	A	G	7: 105,685,692		probably null	Het
Fezf1	A	G	6: 23,247,528	S183P	probably damaging	Het
Irx2	A	G	13: 72,629,129	Y23C	probably damaging	Het
Itga1	A	T	13: 114,974,105	N983K	probably benign	Het
Kifc1	A	G	17: 33,881,456		probably benign	Het
Lrrc14b	T	C	13: 74,361,361	N309S	probably damaging	Het
Lyst	A	T	13: 13,664,116		probably null	Het
Map1b	T	C	13: 99,430,022	T2064A	unknown	Het
Mark3	T	A	12: 111,575,083	N11K	probably benign	Het
Med6	T	A	12: 81,581,875	D80V	possibly damaging	Het
Mfhas1	A	G	8: 35,589,118	E249G	probably damaging	Het
Mroh2b	A	G	15: 4,947,935	I1256M	probably benign	Het
Nkx2-9	G	T	12: 56,611,938	R164S	probably benign	Het
Olfr1181	T	A	2: 88,423,350	N225I	probably benign	Het
Phf10	T	C	17: 14,959,512	D33G	probably null	Het
Plekha5	A	G	6: 140,577,290	M719V	probably damaging	Het
Pln	A	G	10: 53,343,696		probably benign	Het
Prg4	G	A	1: 150,455,101		probably benign	Het
Slc35b3	A	G	13: 38,954,136	L99P	probably damaging	Het
Tbccd1	AT	ATGT	16: 22,834,028		probably null	Het
Ube2j1	C	T	4: 33,045,198	S157L	probably damaging	Het
Zfp462	A	G	4: 55,008,933	T300A	possibly damaging	Het
Zufsp	A	G	10: 33,949,435	M17T	possibly damaging	Het

Other mutations in Zfp668

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00944:Zfp668	APN	7	127867907	missense	probably damaging	1.00
IGL01063:Zfp668	APN	7	127866282	missense	probably damaging	1.00
IGL01909:Zfp668	APN	7	127866822	missense	probably damaging	1.00
IGL02404:Zfp668	APN	7	127866360	missense	probably damaging	1.00
IGL02543:Zfp668	APN	7	127868322	nonsense	probably null
IGL02946:Zfp668	APN	7	127866518	missense	possibly damaging	0.70
R0078:Zfp668	UTSW	7	127868038	missense	possibly damaging	0.94
R1521:Zfp668	UTSW	7	127867080	missense	probably benign	0.09
R1775:Zfp668	UTSW	7	127866606	missense	possibly damaging	0.75
R1873:Zfp668	UTSW	7	127866482	splice site	probably null
R1875:Zfp668	UTSW	7	127866482	splice site	probably null
R2066:Zfp668	UTSW	7	127867031	missense	probably damaging	0.98
R2068:Zfp668	UTSW	7	127866665	missense	probably benign	0.06
R2278:Zfp668	UTSW	7	127866826	missense	probably benign	0.05
R5516:Zfp668	UTSW	7	127867146	missense	probably damaging	1.00
R5569:Zfp668	UTSW	7	127867823	nonsense	probably null
R5875:Zfp668	UTSW	7	127866302	missense	possibly damaging	0.61
R8543:Zfp668	UTSW	7	127867220	missense	probably damaging	1.00
R8822:Zfp668	UTSW	7	127867143	missense	probably damaging	1.00
R8905:Zfp668	UTSW	7	127866426	missense	probably benign	0.04
R9191:Zfp668	UTSW	7	127866486	missense	probably benign
R9217:Zfp668	UTSW	7	127866632	nonsense	probably null
R9655:Zfp668	UTSW	7	127866941	missense	possibly damaging	0.70

Predicted Primers

PCR Primer
(F):5'- CGACTCCAAATACTGCCTGG -3'
(R):5'- ACTAAAGATCCACCAGCGTG -3'

Sequencing Primer
(F):5'- AAAGCCTTGCCTGCTCTCAG -3'
(R):5'- CACCAGCGTGGGCACAC -3'

Posted On

2018-07-23