Mutagenetix > Incidental Mutation

Incidental Mutation 'R6667:Zfp873'

527030

Institutional Source

Beutler Lab

Gene Symbol

Zfp873

Ensembl Gene

ENSMUSG00000061371

Gene Name

zinc finger protein 873

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.095) question?

Stock #

R6667 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

82048123-82064745 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 82060589 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 422 (T422A)

Ref Sequence

ENSEMBL: ENSMUSP00000148219 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105313] [ENSMUST00000209622] [ENSMUST00000210325]

AlphaFold

A0A1B0GT64

Predicted Effect

probably benign
Transcript: ENSMUST00000105313
AA Change: T385A

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000100950
Gene: ENSMUSG00000061371
AA Change: T385A

Domain	Start	End	E-Value	Type
ZnF_C2H2	68	90	1.12e2	SMART
ZnF_C2H2	96	117	4.69e0	SMART
ZnF_C2H2	123	145	2.06e1	SMART
ZnF_C2H2	151	173	5.5e-3	SMART
ZnF_C2H2	179	201	3.69e-4	SMART
ZnF_C2H2	207	229	3.89e-3	SMART
ZnF_C2H2	235	257	9.88e-5	SMART
ZnF_C2H2	263	285	5.59e-4	SMART
ZnF_C2H2	291	313	2.99e-4	SMART
ZnF_C2H2	319	341	1.95e-3	SMART
ZnF_C2H2	347	369	2.75e-3	SMART
ZnF_C2H2	375	397	7.37e-4	SMART
ZnF_C2H2	403	425	2.53e-2	SMART
ZnF_C2H2	431	453	9.08e-4	SMART
ZnF_C2H2	459	481	5.99e-4	SMART
ZnF_C2H2	487	509	1.22e-4	SMART
ZnF_C2H2	515	537	1.82e-3	SMART
ZnF_C2H2	543	565	2.53e-2	SMART
ZnF_C2H2	571	593	9.73e-4	SMART
ZnF_C2H2	599	619	3.13e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000209614

Predicted Effect

probably benign
Transcript: ENSMUST00000209622

Predicted Effect

probably benign
Transcript: ENSMUST00000210325
AA Change: T422A

PolyPhen 2 Score 0.224 (Sensitivity: 0.91; Specificity: 0.88)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000211338

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219748

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.6%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3100002H09Rik	G	T	4: 124,610,642	A39E	probably damaging	Het
Agtr1b	T	A	3: 20,315,749	N231I	possibly damaging	Het
Alpk2	T	C	18: 65,307,740	E661G	probably damaging	Het
Ankrd26	C	T	6: 118,507,788	S1496N	probably benign	Het
Asah2	T	C	19: 31,995,358	N659S	probably benign	Het
Atp12a	T	C	14: 56,384,188	V760A	possibly damaging	Het
Casp2	T	C	6: 42,279,836	C343R	probably damaging	Het
Cblb	T	A	16: 52,152,644	M446K	possibly damaging	Het
Cipc	T	C	12: 86,962,090	V241A	probably benign	Het
Ddit4l	A	G	3: 137,626,121	K83E	probably benign	Het
E430018J23Rik	A	G	7: 127,393,423	M5T	probably benign	Het
Epc2	A	G	2: 49,522,669	T220A	probably damaging	Het
Epha5	T	C	5: 84,071,191	D741G	probably damaging	Het
Flg2	A	T	3: 93,201,761	R365S	possibly damaging	Het
Ggn	A	T	7: 29,172,668	H491L	possibly damaging	Het
Gm21119	T	C	8: 20,621,939	S267P	probably benign	Het
Gm8332	A	T	12: 88,249,705	D132E	unknown	Het
Gpat2	G	C	2: 127,431,918	G294R	possibly damaging	Het
Ighv6-4	A	G	12: 114,406,532	V100A	probably benign	Het
Invs	A	T	4: 48,402,870	Y501F	possibly damaging	Het
Iqcm	G	T	8: 75,753,352	G313W	probably damaging	Het
Jph2	A	G	2: 163,376,286	S157P	probably damaging	Het
Mast4	T	C	13: 102,737,496	E1596G	probably damaging	Het
Mllt6	T	A	11: 97,676,934	L759Q	probably damaging	Het
Nalcn	A	G	14: 123,321,323	L837P	probably damaging	Het
Neb	G	A	2: 52,147,189	T6836I	probably damaging	Het
Nol12	T	A	15: 78,940,080	D133E	probably benign	Het
Olfr1136	A	G	2: 87,693,570	V104A	probably benign	Het
Oxtr	C	A	6: 112,477,099		probably benign	Het
Pcmt1	A	G	10: 7,663,149	L38P	probably damaging	Het
Pik3r2	T	C	8: 70,769,173	Y617C	probably damaging	Het
Prl7a2	T	C	13: 27,661,041	N121D	probably benign	Het
Pvr	G	T	7: 19,905,802	Q380K	probably benign	Het
Rtn2	A	G	7: 19,287,259	E188G	probably benign	Het
Setd4	C	A	16: 93,590,030	R260L	probably benign	Het
Six5	A	G	7: 19,096,569	N374D	probably benign	Het
Slc9b1	T	C	3: 135,371,965	I140T	probably damaging	Het
Supt16	A	G	14: 52,172,063	F797L	probably damaging	Het
Tbata	T	C	10: 61,185,363	L262P	probably damaging	Het
Tti1	A	T	2: 158,008,427	C297*	probably null	Het
Ush1c	C	A	7: 46,225,624	G139C	probably damaging	Het
Vmn1r1	C	T	1: 182,157,777	V108I	probably benign	Het
Vmn2r116	C	T	17: 23,401,092	T600I	probably damaging	Het
Zfp943	G	A	17: 21,992,908	C325Y	probably damaging	Het

Other mutations in Zfp873

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02250:Zfp873	APN	10	82058418	start codon destroyed	probably null	0.45
G1Funyon:Zfp873	UTSW	10	82060879	missense	probably damaging	1.00
R0666:Zfp873	UTSW	10	82060761	missense	possibly damaging	0.75
R1568:Zfp873	UTSW	10	82060279	missense	probably damaging	1.00
R1739:Zfp873	UTSW	10	82060707	missense	probably damaging	1.00
R1848:Zfp873	UTSW	10	82060572	missense	probably benign	0.33
R1892:Zfp873	UTSW	10	82061246	missense	probably damaging	1.00
R2061:Zfp873	UTSW	10	82060157	missense	probably benign	0.01
R3735:Zfp873	UTSW	10	82061181	missense	probably benign	0.15
R4422:Zfp873	UTSW	10	82060874	missense	probably benign	0.13
R4674:Zfp873	UTSW	10	82059980	missense	possibly damaging	0.53
R4839:Zfp873	UTSW	10	82060519	missense	probably damaging	0.98
R5146:Zfp873	UTSW	10	82060224	missense	probably damaging	1.00
R5154:Zfp873	UTSW	10	82060191	missense	possibly damaging	0.54
R5160:Zfp873	UTSW	10	82061042	missense	possibly damaging	0.54
R5811:Zfp873	UTSW	10	82060733	missense	probably damaging	1.00
R6625:Zfp873	UTSW	10	82060304	missense	probably damaging	1.00
R6742:Zfp873	UTSW	10	82058422	missense	probably damaging	1.00
R6878:Zfp873	UTSW	10	82060695	missense	probably benign	0.33
R7055:Zfp873	UTSW	10	82059998	missense	probably damaging	1.00
R7296:Zfp873	UTSW	10	82061237	missense	probably damaging	1.00
R7381:Zfp873	UTSW	10	82060971	missense	probably damaging	1.00
R7448:Zfp873	UTSW	10	82060627	missense	probably damaging	1.00
R7464:Zfp873	UTSW	10	82060376	missense	possibly damaging	0.51
R7470:Zfp873	UTSW	10	82059939	missense	probably benign	0.19
R7640:Zfp873	UTSW	10	82060275	missense	possibly damaging	0.72
R7862:Zfp873	UTSW	10	82060275	missense	probably benign	0.03
R8017:Zfp873	UTSW	10	82060359	missense	probably benign	0.01
R8301:Zfp873	UTSW	10	82060879	missense	probably damaging	1.00
R8765:Zfp873	UTSW	10	82060238	missense	probably damaging	1.00
R8997:Zfp873	UTSW	10	82061156	missense	probably benign	0.03
R9305:Zfp873	UTSW	10	82060680	missense	probably benign	0.00
R9462:Zfp873	UTSW	10	82061297	missense	probably benign	0.31
Z1177:Zfp873	UTSW	10	82061165	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAGAAACCCTATGATTGCACTG -3'
(R):5'- TAGGGCTTCTCTCCAGTGTGATG -3'

Sequencing Primer
(F):5'- ACCCTATGATTGCACTGAATGTGG -3'
(R):5'- TCATGGTATTGAAGACTAGCACGAC -3'

Posted On

2018-07-23