Incidental Mutation 'R6667:Gm8332'
ID 527033
Institutional Source Beutler Lab
Gene Symbol Gm8332
Ensembl Gene ENSMUSG00000095799
Gene Name predicted pseudogene 8332
Synonyms
MMRRC Submission
Accession Numbers
Is this an essential gene? Not available question?
Stock # R6667 (G1)
Quality Score 225.009
Status Not validated
Chromosome 12
Chromosomal Location 88249666-88250100 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to T at 88249705 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 132 (D132E)
Ref Sequence ENSEMBL: ENSMUSP00000136709 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000179468]
AlphaFold no structure available at present
Predicted Effect unknown
Transcript: ENSMUST00000179468
AA Change: D132E
SMART Domains Protein: ENSMUSP00000136709
Gene: ENSMUSG00000095799
AA Change: D132E

DomainStartEndE-ValueType
low complexity region 3 25 N/A INTRINSIC
eIF1a 28 110 2.58e-46 SMART
low complexity region 125 144 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000221854
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.6%
Validation Efficiency 98% (41/42)
Allele List at MGI
Other mutations in this stock
Total: 44 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3100002H09Rik G T 4: 124,610,642 A39E probably damaging Het
Agtr1b T A 3: 20,315,749 N231I possibly damaging Het
Alpk2 T C 18: 65,307,740 E661G probably damaging Het
Ankrd26 C T 6: 118,507,788 S1496N probably benign Het
Asah2 T C 19: 31,995,358 N659S probably benign Het
Atp12a T C 14: 56,384,188 V760A possibly damaging Het
Casp2 T C 6: 42,279,836 C343R probably damaging Het
Cblb T A 16: 52,152,644 M446K possibly damaging Het
Cipc T C 12: 86,962,090 V241A probably benign Het
Ddit4l A G 3: 137,626,121 K83E probably benign Het
E430018J23Rik A G 7: 127,393,423 M5T probably benign Het
Epc2 A G 2: 49,522,669 T220A probably damaging Het
Epha5 T C 5: 84,071,191 D741G probably damaging Het
Flg2 A T 3: 93,201,761 R365S possibly damaging Het
Ggn A T 7: 29,172,668 H491L possibly damaging Het
Gm21119 T C 8: 20,621,939 S267P probably benign Het
Gpat2 G C 2: 127,431,918 G294R possibly damaging Het
Ighv6-4 A G 12: 114,406,532 V100A probably benign Het
Invs A T 4: 48,402,870 Y501F possibly damaging Het
Iqcm G T 8: 75,753,352 G313W probably damaging Het
Jph2 A G 2: 163,376,286 S157P probably damaging Het
Mast4 T C 13: 102,737,496 E1596G probably damaging Het
Mllt6 T A 11: 97,676,934 L759Q probably damaging Het
Nalcn A G 14: 123,321,323 L837P probably damaging Het
Neb G A 2: 52,147,189 T6836I probably damaging Het
Nol12 T A 15: 78,940,080 D133E probably benign Het
Olfr1136 A G 2: 87,693,570 V104A probably benign Het
Oxtr C A 6: 112,477,099 probably benign Het
Pcmt1 A G 10: 7,663,149 L38P probably damaging Het
Pik3r2 T C 8: 70,769,173 Y617C probably damaging Het
Prl7a2 T C 13: 27,661,041 N121D probably benign Het
Pvr G T 7: 19,905,802 Q380K probably benign Het
Rtn2 A G 7: 19,287,259 E188G probably benign Het
Setd4 C A 16: 93,590,030 R260L probably benign Het
Six5 A G 7: 19,096,569 N374D probably benign Het
Slc9b1 T C 3: 135,371,965 I140T probably damaging Het
Supt16 A G 14: 52,172,063 F797L probably damaging Het
Tbata T C 10: 61,185,363 L262P probably damaging Het
Tti1 A T 2: 158,008,427 C297* probably null Het
Ush1c C A 7: 46,225,624 G139C probably damaging Het
Vmn1r1 C T 1: 182,157,777 V108I probably benign Het
Vmn2r116 C T 17: 23,401,092 T600I probably damaging Het
Zfp873 A G 10: 82,060,589 T422A probably benign Het
Zfp943 G A 17: 21,992,908 C325Y probably damaging Het
Other mutations in Gm8332
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1742:Gm8332 UTSW 12 88249683 missense unknown
R3872:Gm8332 UTSW 12 88249706 missense unknown
R3873:Gm8332 UTSW 12 88249706 missense unknown
R3875:Gm8332 UTSW 12 88249706 missense unknown
R5784:Gm8332 UTSW 12 88249755 missense probably damaging 1.00
R6447:Gm8332 UTSW 12 88249724 missense unknown
R7670:Gm8332 UTSW 12 88249754 missense probably benign 0.14
R7852:Gm8332 UTSW 12 88249818 missense probably damaging 1.00
R8508:Gm8332 UTSW 12 88249685 missense unknown
Z1177:Gm8332 UTSW 12 88249802 missense possibly damaging 0.87
Predicted Primers PCR Primer
(F):5'- TGGAAGTCCCTTAACAGTGC -3'
(R):5'- CCAAAATGCTGGGATGTGGAC -3'

Sequencing Primer
(F):5'- AGTGCACACTGCTCTGC -3'
(R):5'- CTGTGCCACATAAGAGGGAAGTTG -3'
Posted On 2018-07-23