Mutagenetix > Incidental Mutation

Incidental Mutation 'R6667:Prl7a2'

527035

Institutional Source

Beutler Lab

Gene Symbol

Prl7a2

Ensembl Gene

ENSMUSG00000046899

Gene Name

prolactin family 7, subfamily a, member 2

Synonyms

PLP-F, Prlpf

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R6667 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

27658584-27668036 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 27661041 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 121 (N121D)

Ref Sequence

ENSEMBL: ENSMUSP00000006660 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006660]

AlphaFold

O54831

Predicted Effect

probably benign
Transcript: ENSMUST00000006660
AA Change: N121D

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000006660
Gene: ENSMUSG00000046899
AA Change: N121D

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	17	244	6.9e-45	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.6%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3100002H09Rik	G	T	4: 124,610,642	A39E	probably damaging	Het
Agtr1b	T	A	3: 20,315,749	N231I	possibly damaging	Het
Alpk2	T	C	18: 65,307,740	E661G	probably damaging	Het
Ankrd26	C	T	6: 118,507,788	S1496N	probably benign	Het
Asah2	T	C	19: 31,995,358	N659S	probably benign	Het
Atp12a	T	C	14: 56,384,188	V760A	possibly damaging	Het
Casp2	T	C	6: 42,279,836	C343R	probably damaging	Het
Cblb	T	A	16: 52,152,644	M446K	possibly damaging	Het
Cipc	T	C	12: 86,962,090	V241A	probably benign	Het
Ddit4l	A	G	3: 137,626,121	K83E	probably benign	Het
E430018J23Rik	A	G	7: 127,393,423	M5T	probably benign	Het
Epc2	A	G	2: 49,522,669	T220A	probably damaging	Het
Epha5	T	C	5: 84,071,191	D741G	probably damaging	Het
Flg2	A	T	3: 93,201,761	R365S	possibly damaging	Het
Ggn	A	T	7: 29,172,668	H491L	possibly damaging	Het
Gm21119	T	C	8: 20,621,939	S267P	probably benign	Het
Gm8332	A	T	12: 88,249,705	D132E	unknown	Het
Gpat2	G	C	2: 127,431,918	G294R	possibly damaging	Het
Ighv6-4	A	G	12: 114,406,532	V100A	probably benign	Het
Invs	A	T	4: 48,402,870	Y501F	possibly damaging	Het
Iqcm	G	T	8: 75,753,352	G313W	probably damaging	Het
Jph2	A	G	2: 163,376,286	S157P	probably damaging	Het
Mast4	T	C	13: 102,737,496	E1596G	probably damaging	Het
Mllt6	T	A	11: 97,676,934	L759Q	probably damaging	Het
Nalcn	A	G	14: 123,321,323	L837P	probably damaging	Het
Neb	G	A	2: 52,147,189	T6836I	probably damaging	Het
Nol12	T	A	15: 78,940,080	D133E	probably benign	Het
Olfr1136	A	G	2: 87,693,570	V104A	probably benign	Het
Oxtr	C	A	6: 112,477,099		probably benign	Het
Pcmt1	A	G	10: 7,663,149	L38P	probably damaging	Het
Pik3r2	T	C	8: 70,769,173	Y617C	probably damaging	Het
Pvr	G	T	7: 19,905,802	Q380K	probably benign	Het
Rtn2	A	G	7: 19,287,259	E188G	probably benign	Het
Setd4	C	A	16: 93,590,030	R260L	probably benign	Het
Six5	A	G	7: 19,096,569	N374D	probably benign	Het
Slc9b1	T	C	3: 135,371,965	I140T	probably damaging	Het
Supt16	A	G	14: 52,172,063	F797L	probably damaging	Het
Tbata	T	C	10: 61,185,363	L262P	probably damaging	Het
Tti1	A	T	2: 158,008,427	C297*	probably null	Het
Ush1c	C	A	7: 46,225,624	G139C	probably damaging	Het
Vmn1r1	C	T	1: 182,157,777	V108I	probably benign	Het
Vmn2r116	C	T	17: 23,401,092	T600I	probably damaging	Het
Zfp873	A	G	10: 82,060,589	T422A	probably benign	Het
Zfp943	G	A	17: 21,992,908	C325Y	probably damaging	Het

Other mutations in Prl7a2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01484:Prl7a2	APN	13	27659208	missense	probably damaging	0.98
IGL02424:Prl7a2	APN	13	27667970	missense	probably null	0.08
IGL02734:Prl7a2	APN	13	27659207	missense	probably benign	0.38
IGL02823:Prl7a2	APN	13	27662751	missense	possibly damaging	0.95
PIT4260001:Prl7a2	UTSW	13	27659276	nonsense	probably null
R0733:Prl7a2	UTSW	13	27662688	missense	probably damaging	1.00
R1371:Prl7a2	UTSW	13	27662767	missense	probably benign	0.01
R1778:Prl7a2	UTSW	13	27659271	missense	probably damaging	0.98
R1857:Prl7a2	UTSW	13	27659180	nonsense	probably null
R2063:Prl7a2	UTSW	13	27660887	missense	probably damaging	0.98
R2064:Prl7a2	UTSW	13	27660887	missense	probably damaging	0.98
R2065:Prl7a2	UTSW	13	27660887	missense	probably damaging	0.98
R2067:Prl7a2	UTSW	13	27660887	missense	probably damaging	0.98
R2068:Prl7a2	UTSW	13	27660887	missense	probably damaging	0.98
R2176:Prl7a2	UTSW	13	27659106	missense	probably benign	0.10
R2213:Prl7a2	UTSW	13	27665068	missense	probably benign	0.06
R4111:Prl7a2	UTSW	13	27665067	missense	possibly damaging	0.96
R4459:Prl7a2	UTSW	13	27665996	missense	probably benign	0.21
R4483:Prl7a2	UTSW	13	27660947	missense	possibly damaging	0.80
R4722:Prl7a2	UTSW	13	27660875	missense	probably damaging	1.00
R5360:Prl7a2	UTSW	13	27659160	missense	probably benign	0.22
R5778:Prl7a2	UTSW	13	27661000	nonsense	probably null
R7107:Prl7a2	UTSW	13	27659093	missense	possibly damaging	0.89
R7600:Prl7a2	UTSW	13	27659281	missense	possibly damaging	0.63
R8298:Prl7a2	UTSW	13	27661011	missense	probably benign	0.00
R8447:Prl7a2	UTSW	13	27665958	missense	possibly damaging	0.72
R9009:Prl7a2	UTSW	13	27666011	missense	probably damaging	1.00
R9331:Prl7a2	UTSW	13	27665079	missense	probably damaging	1.00
R9624:Prl7a2	UTSW	13	27665886	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CACCGAGTTGAGTATACTCTGG -3'
(R):5'- GGTCTAATGTCAGAATTGCATATGC -3'

Sequencing Primer
(F):5'- CTGGGTGTACTCAAAAAGCTCTGC -3'
(R):5'- GCTTGTCTCATAGAAAACCAA -3'

Posted On

2018-07-23