Mutagenetix > Incidental Mutation

Incidental Mutation 'R6671:Gm3404'

527134

Institutional Source

Beutler Lab

Gene Symbol

Gm3404

Ensembl Gene

ENSMUSG00000079091

Gene Name

predicted gene 3404

Synonyms

MMRRC Submission

044791-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.177) question?

Stock #

R6671 (G1)

Quality Score

98.0078

Status

Not validated

Chromosome

Chromosomal Location

146462611-146465363 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 146464487 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 163 (R163S)

Ref Sequence

ENSEMBL: ENSMUSP00000106226 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110596]

AlphaFold

Q9D506

Predicted Effect

probably benign
Transcript: ENSMUST00000110596
AA Change: R163S

PolyPhen 2 Score 0.051 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000106226
Gene: ENSMUSG00000079091
AA Change: R163S

Domain	Start	End	E-Value	Type
RasGEFN	66	181	2.8e-4	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.0%

Validation Efficiency

97% (32/33)

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430401F13Rik	T	C	6: 131,528,313 (GRCm39)		probably null	Het
Abce1	A	G	8: 80,415,806 (GRCm39)	V404A	probably benign	Het
Cpd	T	A	11: 76,686,359 (GRCm39)	I990F	probably damaging	Het
Ddhd1	CA	C	14: 45,894,689 (GRCm39)		probably null	Het
Dnajb6	A	G	5: 29,953,418 (GRCm39)	E17G	probably damaging	Het
Fastk	T	C	5: 24,646,607 (GRCm39)	D308G	probably damaging	Het
Fkbp14	A	G	6: 54,556,662 (GRCm39)	Y69H	probably damaging	Het
Gldn	T	C	9: 54,245,691 (GRCm39)	L414P	probably damaging	Het
Glmn	T	A	5: 107,697,280 (GRCm39)	M487L	probably benign	Het
Gm5591	T	G	7: 38,219,523 (GRCm39)	D450A	possibly damaging	Het
Gucy1b1	T	C	3: 81,941,715 (GRCm39)	T575A	probably benign	Het
Hydin	T	A	8: 111,327,950 (GRCm39)	V4819D	probably damaging	Het
Ikbip	A	G	10: 90,932,469 (GRCm39)		probably null	Het
Mertk	G	T	2: 128,593,943 (GRCm39)		probably null	Het
Mfsd1	T	C	3: 67,492,995 (GRCm39)	V93A	possibly damaging	Het
Myh11	A	T	16: 14,044,480 (GRCm39)	M641K	possibly damaging	Het
Myo3a	A	T	2: 22,299,333 (GRCm39)	N269Y	probably damaging	Het
Nisch	A	T	14: 30,926,420 (GRCm39)		probably benign	Het
Otof	A	G	5: 30,576,877 (GRCm39)	V125A	probably benign	Het
Pla2g4a	A	G	1: 149,763,382 (GRCm39)	I93T	probably benign	Het
Prrc2c	A	G	1: 162,525,154 (GRCm39)	I484T	probably damaging	Het
Qrich1	T	A	9: 108,410,985 (GRCm39)	I170N	probably benign	Het
Rb1	A	T	14: 73,434,706 (GRCm39)	M904K	probably damaging	Het
Rgs11	A	T	17: 26,427,272 (GRCm39)	K399M	probably damaging	Het
Tmprss13	C	T	9: 45,254,529 (GRCm39)	T432M	probably damaging	Het
Tpp1	C	T	7: 105,398,814 (GRCm39)	R205H	probably benign	Het
Vps53	A	G	11: 76,025,332 (GRCm39)	Y171H	probably damaging	Het
Zbtb8b	C	T	4: 129,321,577 (GRCm39)	R395Q	probably damaging	Het
Zfp81	A	T	17: 33,554,413 (GRCm39)	C134S	probably benign	Het
Zranb1	A	G	7: 132,573,042 (GRCm39)	D403G	probably damaging	Het

Other mutations in Gm3404

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00505:Gm3404	APN	5	146,465,095 (GRCm39)	missense	probably damaging	0.98
IGL01611:Gm3404	APN	5	146,465,157 (GRCm39)	missense	possibly damaging	0.46
IGL02721:Gm3404	APN	5	146,463,738 (GRCm39)	nonsense	probably null
IGL03134:Gm3404	UTSW	5	146,463,706 (GRCm39)	missense	probably benign
R1758:Gm3404	UTSW	5	146,463,036 (GRCm39)	missense	probably benign	0.02
R6324:Gm3404	UTSW	5	146,464,917 (GRCm39)	missense	possibly damaging	0.79
R6641:Gm3404	UTSW	5	146,464,518 (GRCm39)	missense	probably damaging	0.97
R7042:Gm3404	UTSW	5	146,462,969 (GRCm39)	missense	probably benign
R8331:Gm3404	UTSW	5	146,462,759 (GRCm39)	missense	probably damaging	0.97
R8395:Gm3404	UTSW	5	146,462,724 (GRCm39)	missense	possibly damaging	0.79
R8547:Gm3404	UTSW	5	146,465,108 (GRCm39)	missense	possibly damaging	0.92
R8874:Gm3404	UTSW	5	146,464,953 (GRCm39)	missense	possibly damaging	0.95
R9280:Gm3404	UTSW	5	146,462,756 (GRCm39)	missense	possibly damaging	0.46
R9671:Gm3404	UTSW	5	146,463,031 (GRCm39)	missense	probably benign	0.04
Z1177:Gm3404	UTSW	5	146,463,026 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GGCCACTATACTCATCTGCAGAC -3'
(R):5'- AAAGCTCTGGAGAATACCCTGC -3'

Sequencing Primer
(F):5'- TATACTCATCTGCAGACAGGCTGG -3'
(R):5'- TCTGGAGAATACCCTGCTGGAATC -3'

Posted On

2018-07-23