Mutagenetix > Incidental Mutation

Incidental Mutation 'R6588:Zfp64'

527414

Institutional Source

Beutler Lab

Gene Symbol

Zfp64

Ensembl Gene

ENSMUSG00000027551

Gene Name

zinc finger protein 64

Synonyms

MMRRC Submission

044712-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.863) question?

Stock #

R6588 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

168735251-168797507 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 168768827 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 262 (C262S)

Ref Sequence

ENSEMBL: ENSMUSP00000085285 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087971] [ENSMUST00000109162]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000087971
AA Change: C262S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000085285
Gene: ENSMUSG00000027551
AA Change: C262S

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	257	279	3.89e-3	SMART
ZnF_C2H2	285	308	6.32e-3	SMART
ZnF_C2H2	313	335	2.05e-2	SMART
ZnF_C2H2	341	363	1.95e-3	SMART
ZnF_C2H2	369	392	1.36e-2	SMART
ZnF_C2H2	423	445	1.47e-3	SMART
low complexity region	503	522	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000109162

SMART Domains

Protein: ENSMUSP00000104790
Gene: ENSMUSG00000027551

Domain	Start	End	E-Value	Type
ZnF_C2H2	31	51	5.54e1	SMART
low complexity region	71	93	N/A	INTRINSIC
ZnF_C2H2	142	167	1.68e1	SMART
ZnF_C2H2	173	195	2.24e-3	SMART
ZnF_C2H2	201	223	1.58e-3	SMART
ZnF_C2H2	229	251	2.24e-3	SMART
ZnF_C2H2	297	322	5.2e0	SMART
ZnF_C2H2	328	350	4.17e-3	SMART
ZnF_C2H2	356	378	6.78e-3	SMART
ZnF_C2H2	384	406	2.24e-3	SMART
ZnF_C2H2	412	434	1.79e-2	SMART
ZnF_C2H2	440	463	9.58e-3	SMART
ZnF_C2H2	465	487	3.78e-1	SMART
ZnF_C2H2	493	515	3.89e-3	SMART
ZnF_C2H2	521	544	1.92e-2	SMART
ZnF_C2H2	578	600	5.34e-1	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 19 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	C	13: 77,340,766 (GRCm39)	S132P	probably benign	Het
Bean1	CT	C	8: 104,908,664 (GRCm39)		probably null	Homo
Clcn3	T	C	8: 61,367,861 (GRCm39)	I763V	probably benign	Het
Cpsf1	A	G	15: 76,481,022 (GRCm39)	Y1254H	probably damaging	Het
Erc1	C	T	6: 119,552,687 (GRCm39)	A1088T	possibly damaging	Het
Fhl4	A	G	10: 84,933,971 (GRCm39)	V270A	possibly damaging	Het
Ghr	T	C	15: 3,349,750 (GRCm39)	E476G	probably benign	Het
Ighv1-84	T	A	12: 115,944,371 (GRCm39)	Q101L	probably benign	Het
Itfg1	T	C	8: 86,462,759 (GRCm39)	I455V	probably benign	Het
Kitl	T	A	10: 99,899,954 (GRCm39)	N86K	probably damaging	Het
Kmt2c	C	T	5: 25,528,787 (GRCm39)	G1614E	probably damaging	Het
Lrrd1	A	G	5: 3,901,386 (GRCm39)	S564G	probably benign	Het
Lsm3	GATATATA	GATATATATA	6: 91,496,617 (GRCm39)		probably null	Het
Macroh2a1	C	T	13: 56,252,302 (GRCm39)	G97D	possibly damaging	Het
Mms19	C	T	19: 41,954,715 (GRCm39)	R68Q	probably damaging	Het
Or10aa1	G	T	1: 173,869,844 (GRCm39)	M109I	probably benign	Het
Or1o1	G	A	17: 37,716,796 (GRCm39)	R119H	probably benign	Het
Or7e173	G	T	9: 19,939,162 (GRCm39)	P24Q	probably damaging	Het
Urb2	G	T	8: 124,757,864 (GRCm39)	E1190D	probably damaging	Het

Other mutations in Zfp64

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00155:Zfp64	APN	2	168,768,601 (GRCm39)	missense	probably benign	0.06
IGL01479:Zfp64	APN	2	168,793,599 (GRCm39)	missense	probably damaging	1.00
IGL02320:Zfp64	APN	2	168,768,118 (GRCm39)	missense	probably damaging	0.98
IGL03411:Zfp64	APN	2	168,793,462 (GRCm39)	splice site	probably null
PIT4362001:Zfp64	UTSW	2	168,767,735 (GRCm39)	missense	probably benign	0.23
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0033:Zfp64	UTSW	2	168,767,635 (GRCm39)	missense	possibly damaging	0.94
R0230:Zfp64	UTSW	2	168,754,150 (GRCm39)	splice site	probably benign
R0325:Zfp64	UTSW	2	168,767,960 (GRCm39)	missense	probably benign	0.00
R0689:Zfp64	UTSW	2	168,777,121 (GRCm39)	splice site	probably benign
R1741:Zfp64	UTSW	2	168,768,238 (GRCm39)	missense	probably benign	0.05
R2054:Zfp64	UTSW	2	168,767,728 (GRCm39)	missense	probably damaging	1.00
R2133:Zfp64	UTSW	2	168,782,663 (GRCm39)	missense	probably damaging	1.00
R2254:Zfp64	UTSW	2	168,768,662 (GRCm39)	missense	probably damaging	0.96
R4093:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4094:Zfp64	UTSW	2	168,767,855 (GRCm39)	missense	probably benign	0.00
R4257:Zfp64	UTSW	2	168,768,298 (GRCm39)	missense	probably damaging	1.00
R4630:Zfp64	UTSW	2	168,768,463 (GRCm39)	missense	possibly damaging	0.94
R4642:Zfp64	UTSW	2	168,776,851 (GRCm39)	missense	probably benign	0.22
R4817:Zfp64	UTSW	2	168,768,032 (GRCm39)	missense	probably benign
R4880:Zfp64	UTSW	2	168,736,297 (GRCm39)	missense	probably damaging	1.00
R5051:Zfp64	UTSW	2	168,768,304 (GRCm39)	missense	probably damaging	0.98
R5155:Zfp64	UTSW	2	168,748,885 (GRCm39)	missense	probably benign	0.17
R5562:Zfp64	UTSW	2	168,767,642 (GRCm39)	missense	probably benign	0.00
R5619:Zfp64	UTSW	2	168,741,735 (GRCm39)	missense	probably damaging	0.97
R5619:Zfp64	UTSW	2	168,741,734 (GRCm39)	missense	probably damaging	0.99
R5620:Zfp64	UTSW	2	168,741,888 (GRCm39)	missense	possibly damaging	0.70
R5919:Zfp64	UTSW	2	168,768,419 (GRCm39)	missense	probably benign	0.05
R6156:Zfp64	UTSW	2	168,768,088 (GRCm39)	missense	probably benign	0.34
R6364:Zfp64	UTSW	2	168,754,186 (GRCm39)	missense	probably damaging	1.00
R6488:Zfp64	UTSW	2	168,777,129 (GRCm39)	critical splice donor site	probably null
R6512:Zfp64	UTSW	2	168,735,997 (GRCm39)	missense	probably benign	0.00
R7103:Zfp64	UTSW	2	168,768,357 (GRCm39)	missense	probably benign	0.00
R7313:Zfp64	UTSW	2	168,741,810 (GRCm39)	missense	probably damaging	1.00
R7470:Zfp64	UTSW	2	168,767,731 (GRCm39)	missense	probably damaging	1.00
R7529:Zfp64	UTSW	2	168,735,992 (GRCm39)	missense	probably benign
R7560:Zfp64	UTSW	2	168,767,585 (GRCm39)	missense	probably damaging	1.00
R7963:Zfp64	UTSW	2	168,793,538 (GRCm39)	missense	probably benign	0.32
R8037:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8038:Zfp64	UTSW	2	168,741,932 (GRCm39)	missense	probably damaging	1.00
R8825:Zfp64	UTSW	2	168,793,552 (GRCm39)	missense	probably benign
R8840:Zfp64	UTSW	2	168,768,635 (GRCm39)	missense	probably benign
R8891:Zfp64	UTSW	2	168,797,083 (GRCm39)	start codon destroyed	probably null	0.37
R9062:Zfp64	UTSW	2	168,767,747 (GRCm39)	missense	probably benign
R9592:Zfp64	UTSW	2	168,768,118 (GRCm39)	missense	probably damaging	1.00
R9681:Zfp64	UTSW	2	168,793,680 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGAACAGGAGTAGCTGCACTC -3'
(R):5'- AAGAGAATCCCAAGGGCTGC -3'

Sequencing Primer
(F):5'- TAGCTGCACTCGGGACACTTC -3'
(R):5'- AAGGGCTGCTGGGAGCTG -3'

Posted On

2018-07-23