Mutagenetix > Incidental Mutations

Incidental Mutation 'R6662:Zfp786'

528017

Institutional Source

Beutler Lab

Gene Symbol

Zfp786

Ensembl Gene

ENSMUSG00000051499

Gene Name

zinc finger protein 786

Synonyms

A730012O14Rik

MMRRC Submission

Accession Numbers

Genbank: NM_177882; MGI: 3026883

Is this an essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R6662 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

47819266-47830867 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 47826986 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Aspartic acid at position 41 (N41D)

Ref Sequence

ENSEMBL: ENSMUSP00000057495 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058844]

AlphaFold

Q8BV42

Predicted Effect

probably damaging
Transcript: ENSMUST00000058844
AA Change: N41D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000057495
Gene: ENSMUSG00000051499
AA Change: N41D

Domain	Start	End	E-Value	Type
KRAB	9	69	2.6e-22	SMART
low complexity region	156	164	N/A	INTRINSIC
ZnF_C2H2	240	262	5.34e-1	SMART
ZnF_C2H2	268	295	1.41e2	SMART
ZnF_C2H2	420	440	1.21e2	SMART
ZnF_C2H2	448	470	3.52e-1	SMART
ZnF_C2H2	476	498	1.89e-1	SMART
ZnF_C2H2	504	526	1.69e-3	SMART
ZnF_C2H2	532	554	5.59e-4	SMART
ZnF_C2H2	560	582	4.79e-3	SMART
ZnF_C2H2	588	610	3.69e-4	SMART
ZnF_C2H2	616	638	3.63e-3	SMART
ZnF_C2H2	644	665	5.92e0	SMART
ZnF_C2H2	671	693	2.57e-3	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	1.45e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143979

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204984

Meta Mutation Damage Score

0.7991

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.8%

Validation Efficiency

100% (46/46)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim1	A	G	19: 57,073,853		probably null	Het
Acox1	A	G	11: 116,175,323	Y418H	probably damaging	Het
Akr1b3	C	T	6: 34,310,004	V206M	possibly damaging	Het
Aldh3a1	G	A	11: 61,214,655	V196I	probably benign	Het
Aox3	A	G	1: 58,118,615	K44E	probably damaging	Het
Bad	T	A	19: 6,951,070		probably benign	Het
BC034090	G	T	1: 155,226,339	Q60K	possibly damaging	Het
Casp6	A	G	3: 129,912,226	T181A	probably benign	Het
Catsperg2	G	A	7: 29,719,513		probably benign	Het
Ccdc14	T	C	16: 34,690,794	L46P	probably damaging	Het
Ces1b	A	G	8: 93,064,069	L364S	probably benign	Het
Cfap45	T	C	1: 172,529,850	I15T	probably benign	Het
D730048I06Rik	C	A	9: 35,790,000	R6M	possibly damaging	Het
Dph5	G	A	3: 115,928,556	E228K	probably benign	Het
Fat4	G	T	3: 38,956,821	L2023F	possibly damaging	Het
Garem1	T	C	18: 21,148,247	N351D	probably benign	Het
Gm14124	G	A	2: 150,266,252		probably null	Het
Grm2	C	T	9: 106,648,053	A488T	probably benign	Het
Ifit3b	A	G	19: 34,611,937	E171G	probably damaging	Het
Il1rn	A	T	2: 24,336,875		probably null	Het
Itih5	A	T	2: 10,249,181	I748F	probably benign	Het
Kcnh5	C	A	12: 75,007,611	D520Y	probably damaging	Het
Mgat5	C	A	1: 127,469,237	H574N	probably damaging	Het
Moxd1	A	C	10: 24,284,760	D437A	probably damaging	Het
Mybpc2	A	G	7: 44,506,166	F888L	probably benign	Het
Ncs1	T	A	2: 31,287,360	L183Q	probably damaging	Het
Neto2	A	T	8: 85,663,215	D206E	probably damaging	Het
Omp	A	G	7: 98,145,339	L27P	probably damaging	Het
Oxsm	A	G	14: 16,242,287	S161P	probably benign	Het
Pde4b	A	G	4: 102,601,898	I381M	possibly damaging	Het
Pramel5	A	T	4: 144,273,105	N137K	probably benign	Het
Prss33	T	C	17: 23,833,960	S247G	probably damaging	Het
Rassf9	T	A	10: 102,546,038	L425Q	possibly damaging	Het
Setx	A	T	2: 29,158,114	D1909V	probably damaging	Het
Slc26a3	A	T	12: 31,457,346	K402*	probably null	Het
Slco1a6	G	A	6: 142,133,215	T118I	probably damaging	Het
Syne1	A	G	10: 5,128,416	L6769P	probably damaging	Het
Tas2r107	A	T	6: 131,659,489	V199D	possibly damaging	Het
Tchp	A	G	5: 114,720,015		probably null	Het
Trdn	A	T	10: 33,474,487	N684I	probably damaging	Het
Trio	G	T	15: 27,854,996	T700K	probably benign	Het
Ttn	C	T	2: 76,755,898	V20084I	probably benign	Het
Ubl3	A	T	5: 148,509,306	Y62*	probably null	Het
Uckl1	A	G	2: 181,573,260	Y267H	possibly damaging	Het
Zfp983	T	C	17: 21,662,085	S310P	probably damaging	Het

Other mutations in Zfp786

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Zfp786	APN	6	47819671	makesense	probably null
IGL02442:Zfp786	APN	6	47821367	missense	probably benign	0.00
IGL02674:Zfp786	APN	6	47820493	missense	probably benign	0.22
IGL02814:Zfp786	APN	6	47819841	missense	probably damaging	1.00
IGL03072:Zfp786	APN	6	47821243	missense	probably benign	0.01
IGL03294:Zfp786	APN	6	47821324	nonsense	probably null
IGL03393:Zfp786	APN	6	47821524	missense	possibly damaging	0.88
3-1:Zfp786	UTSW	6	47820445	missense	probably damaging	1.00
PIT4581001:Zfp786	UTSW	6	47819822	missense	probably damaging	1.00
R1071:Zfp786	UTSW	6	47821305	missense	possibly damaging	0.91
R1426:Zfp786	UTSW	6	47825079	missense	probably benign	0.17
R1976:Zfp786	UTSW	6	47819757	missense	probably damaging	1.00
R2114:Zfp786	UTSW	6	47826997	missense	probably damaging	1.00
R2115:Zfp786	UTSW	6	47826997	missense	probably damaging	1.00
R2117:Zfp786	UTSW	6	47826997	missense	probably damaging	1.00
R2176:Zfp786	UTSW	6	47820971	missense	possibly damaging	0.65
R2304:Zfp786	UTSW	6	47820699	missense	probably damaging	1.00
R3110:Zfp786	UTSW	6	47820226	missense	probably damaging	1.00
R3112:Zfp786	UTSW	6	47820226	missense	probably damaging	1.00
R3623:Zfp786	UTSW	6	47821423	missense	probably benign	0.00
R4654:Zfp786	UTSW	6	47820934	missense	probably benign	0.01
R4741:Zfp786	UTSW	6	47820691	missense	probably damaging	1.00
R4927:Zfp786	UTSW	6	47820153	missense	probably benign	0.43
R4936:Zfp786	UTSW	6	47821268	nonsense	probably null
R5084:Zfp786	UTSW	6	47820019	missense	probably benign	0.00
R5445:Zfp786	UTSW	6	47819685	missense	probably damaging	1.00
R7290:Zfp786	UTSW	6	47819995	missense	probably damaging	1.00
R7561:Zfp786	UTSW	6	47819733	missense	probably benign	0.02
R8038:Zfp786	UTSW	6	47821254	missense	probably benign	0.01
R8090:Zfp786	UTSW	6	47820009	missense	probably damaging	1.00
R8250:Zfp786	UTSW	6	47820795	missense	possibly damaging	0.85
R8438:Zfp786	UTSW	6	47820000	missense	probably damaging	1.00
R8516:Zfp786	UTSW	6	47820543	missense	probably damaging	1.00
R8868:Zfp786	UTSW	6	47825081	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AGGACAAACCGGTGATGTATAC -3'
(R):5'- AACAGCTGTCCACTCTGTG -3'

Sequencing Primer
(F):5'- GACAAACCGGTGATGTATACATTAG -3'
(R):5'- GTCCACTCTGTGAGTCTCAAATTAC -3'

Posted On

2018-07-24