Incidental Mutation 'R6662:Prss33'
ID528038
Institutional Source Beutler Lab
Gene Symbol Prss33
Ensembl Gene ENSMUSG00000049620
Gene Nameprotease, serine 33
Synonymstryptase-6, mT6, Eos
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.061) question?
Stock #R6662 (G1)
Quality Score225.009
Status Validated
Chromosome17
Chromosomal Location23833360-23836056 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 23833960 bp
ZygosityHeterozygous
Amino Acid Change Serine to Glycine at position 247 (S247G)
Ref Sequence ENSEMBL: ENSMUSP00000059491 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000024926] [ENSMUST00000059906] [ENSMUST00000069579] [ENSMUST00000115444] [ENSMUST00000122936]
Predicted Effect probably benign
Transcript: ENSMUST00000024926
SMART Domains Protein: ENSMUSP00000024926
Gene: ENSMUSG00000024114

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
Tryp_SPc 52 289 2.48e-79 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000059906
AA Change: S247G

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000059491
Gene: ENSMUSG00000049620
AA Change: S247G

DomainStartEndE-ValueType
signal peptide 1 24 N/A INTRINSIC
Tryp_SPc 33 271 9.03e-91 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000069579
SMART Domains Protein: ENSMUSP00000066210
Gene: ENSMUSG00000055839

DomainStartEndE-ValueType
UBQ 3 80 5.1e-11 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000115444
SMART Domains Protein: ENSMUSP00000111104
Gene: ENSMUSG00000049620

DomainStartEndE-ValueType
Tryp_SPc 15 253 4.5e-93 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000122936
SMART Domains Protein: ENSMUSP00000120141
Gene: ENSMUSG00000024114

DomainStartEndE-ValueType
Tryp_SPc 12 249 2.48e-79 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000164140
Meta Mutation Damage Score 0.8479 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.2%
  • 20x: 94.8%
Validation Efficiency 100% (46/46)
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ablim1 A G 19: 57,073,853 probably null Het
Acox1 A G 11: 116,175,323 Y418H probably damaging Het
Akr1b3 C T 6: 34,310,004 V206M possibly damaging Het
Aldh3a1 G A 11: 61,214,655 V196I probably benign Het
Aox3 A G 1: 58,118,615 K44E probably damaging Het
Bad T A 19: 6,951,070 probably benign Het
BC034090 G T 1: 155,226,339 Q60K possibly damaging Het
Casp6 A G 3: 129,912,226 T181A probably benign Het
Catsperg2 G A 7: 29,719,513 probably benign Het
Ccdc14 T C 16: 34,690,794 L46P probably damaging Het
Ces1b A G 8: 93,064,069 L364S probably benign Het
Cfap45 T C 1: 172,529,850 I15T probably benign Het
D730048I06Rik C A 9: 35,790,000 R6M possibly damaging Het
Dph5 G A 3: 115,928,556 E228K probably benign Het
Fat4 G T 3: 38,956,821 L2023F possibly damaging Het
Garem1 T C 18: 21,148,247 N351D probably benign Het
Gm14124 G A 2: 150,266,252 probably null Het
Grm2 C T 9: 106,648,053 A488T probably benign Het
Ifit3b A G 19: 34,611,937 E171G probably damaging Het
Il1rn A T 2: 24,336,875 probably null Het
Itih5 A T 2: 10,249,181 I748F probably benign Het
Kcnh5 C A 12: 75,007,611 D520Y probably damaging Het
Mgat5 C A 1: 127,469,237 H574N probably damaging Het
Moxd1 A C 10: 24,284,760 D437A probably damaging Het
Mybpc2 A G 7: 44,506,166 F888L probably benign Het
Ncs1 T A 2: 31,287,360 L183Q probably damaging Het
Neto2 A T 8: 85,663,215 D206E probably damaging Het
Omp A G 7: 98,145,339 L27P probably damaging Het
Oxsm A G 14: 16,242,287 S161P probably benign Het
Pde4b A G 4: 102,601,898 I381M possibly damaging Het
Pramel5 A T 4: 144,273,105 N137K probably benign Het
Rassf9 T A 10: 102,546,038 L425Q possibly damaging Het
Setx A T 2: 29,158,114 D1909V probably damaging Het
Slc26a3 A T 12: 31,457,346 K402* probably null Het
Slco1a6 G A 6: 142,133,215 T118I probably damaging Het
Syne1 A G 10: 5,128,416 L6769P probably damaging Het
Tas2r107 A T 6: 131,659,489 V199D possibly damaging Het
Tchp A G 5: 114,720,015 probably null Het
Trdn A T 10: 33,474,487 N684I probably damaging Het
Trio G T 15: 27,854,996 T700K probably benign Het
Ttn C T 2: 76,755,898 V20084I probably benign Het
Ubl3 A T 5: 148,509,306 Y62* probably null Het
Uckl1 A G 2: 181,573,260 Y267H possibly damaging Het
Zfp786 T C 6: 47,826,986 N41D probably damaging Het
Zfp983 T C 17: 21,662,085 S310P probably damaging Het
Other mutations in Prss33
AlleleSourceChrCoordTypePredicted EffectPPH Score
Scissorhands UTSW 17 23834702 missense probably benign 0.00
R0506:Prss33 UTSW 17 23835105 missense probably benign 0.00
R1201:Prss33 UTSW 17 23835110 nonsense probably null
R1478:Prss33 UTSW 17 23835098 missense probably damaging 1.00
R1652:Prss33 UTSW 17 23835141 missense probably benign 0.00
R1652:Prss33 UTSW 17 23835142 missense probably benign 0.10
R1662:Prss33 UTSW 17 23834811 unclassified probably null
R1994:Prss33 UTSW 17 23834198 missense probably damaging 0.99
R2151:Prss33 UTSW 17 23834843 missense probably damaging 1.00
R2153:Prss33 UTSW 17 23834843 missense probably damaging 1.00
R2154:Prss33 UTSW 17 23834843 missense probably damaging 1.00
R5002:Prss33 UTSW 17 23835358 unclassified probably benign
R6648:Prss33 UTSW 17 23834702 missense probably benign 0.00
R6801:Prss33 UTSW 17 23834839 missense possibly damaging 0.88
R7726:Prss33 UTSW 17 23834229 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TCTCAGCTTCATGTGAGAAAGAG -3'
(R):5'- AGGTGTGTCTGCTAACCCTC -3'

Sequencing Primer
(F):5'- CTTCATGTGAGAAAGAGGGGAATGTG -3'
(R):5'- GAATTGGTTCCCCAGCCAC -3'
Posted On2018-07-24