Incidental Mutation 'R6652:Cfh'
ID 528079
Institutional Source Beutler Lab
Gene Symbol Cfh
Ensembl Gene ENSMUSG00000026365
Gene Name complement component factor h
Synonyms Sas-1, Mud-1, Sas1
MMRRC Submission 044773-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.105) question?
Stock # R6652 (G1)
Quality Score 225.009
Status Validated
Chromosome 1
Chromosomal Location 140013593-140111149 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 140071806 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Asparagine at position 294 (I294N)
Ref Sequence ENSEMBL: ENSMUSP00000107608 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000066859] [ENSMUST00000111976] [ENSMUST00000111977] [ENSMUST00000123238] [ENSMUST00000192880]
AlphaFold P06909
Predicted Effect probably benign
Transcript: ENSMUST00000066859
AA Change: I276N

PolyPhen 2 Score 0.062 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000066677
Gene: ENSMUSG00000026365
AA Change: I276N

DomainStartEndE-ValueType
CCP 21 80 7.75e-8 SMART
CCP 85 141 2.17e-11 SMART
CCP 146 205 7.5e-15 SMART
CCP 210 262 6.29e-8 SMART
CCP 267 320 2.04e-7 SMART
CCP 325 385 6.35e-4 SMART
CCP 389 442 1.15e-10 SMART
CCP 448 505 3.62e-8 SMART
CCP 509 564 6.45e-5 SMART
CCP 569 622 5.56e-9 SMART
CCP 629 683 3.45e-14 SMART
CCP 690 743 1.82e-13 SMART
CCP 752 802 6.59e-1 SMART
CCP 808 861 1.04e-8 SMART
CCP 867 931 4.66e-11 SMART
CCP 936 989 3.9e-13 SMART
CCP 994 1048 1.4e-14 SMART
CCP 1053 1107 2.09e-13 SMART
CCP 1114 1168 8.04e-15 SMART
CCP 1172 1233 5.57e0 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000111976
AA Change: I294N

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
SMART Domains Protein: ENSMUSP00000107607
Gene: ENSMUSG00000026365
AA Change: I294N

DomainStartEndE-ValueType
CCP 39 98 7.75e-8 SMART
CCP 103 159 2.17e-11 SMART
CCP 164 223 7.5e-15 SMART
CCP 228 280 6.29e-8 SMART
CCP 285 338 2.04e-7 SMART
CCP 343 403 6.35e-4 SMART
CCP 407 460 1.15e-10 SMART
CCP 466 523 3.62e-8 SMART
CCP 527 582 6.45e-5 SMART
CCP 587 640 5.56e-9 SMART
CCP 647 701 3.45e-14 SMART
CCP 708 761 1.82e-13 SMART
CCP 770 820 6.59e-1 SMART
CCP 826 879 1.04e-8 SMART
CCP 885 949 4.66e-11 SMART
CCP 954 1007 3.9e-13 SMART
CCP 1012 1066 1.4e-14 SMART
CCP 1071 1125 2.09e-13 SMART
CCP 1132 1186 8.04e-15 SMART
CCP 1190 1251 5.57e0 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000111977
AA Change: I294N

PolyPhen 2 Score 0.394 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000107608
Gene: ENSMUSG00000026365
AA Change: I294N

DomainStartEndE-ValueType
CCP 21 80 7.75e-8 SMART
CCP 85 141 2.17e-11 SMART
CCP 146 205 7.5e-15 SMART
CCP 210 262 6.29e-8 SMART
CCP 267 320 2.04e-7 SMART
CCP 325 385 6.35e-4 SMART
CCP 389 442 1.15e-10 SMART
CCP 448 505 3.62e-8 SMART
CCP 509 564 6.45e-5 SMART
CCP 572 626 3.45e-14 SMART
CCP 633 686 1.82e-13 SMART
CCP 695 745 6.59e-1 SMART
CCP 751 804 1.04e-8 SMART
CCP 810 874 4.66e-11 SMART
CCP 879 932 3.9e-13 SMART
CCP 937 991 1.4e-14 SMART
CCP 996 1050 2.09e-13 SMART
CCP 1057 1111 8.04e-15 SMART
CCP 1115 1176 5.57e0 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000123238
AA Change: I276N

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000115166
Gene: ENSMUSG00000026365
AA Change: I276N

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
CCP 21 80 7.75e-8 SMART
CCP 85 141 2.17e-11 SMART
CCP 146 205 7.5e-15 SMART
CCP 210 262 6.29e-8 SMART
CCP 267 320 2.04e-7 SMART
CCP 325 385 6.35e-4 SMART
CCP 389 442 1.15e-10 SMART
CCP 448 505 3.62e-8 SMART
CCP 509 564 6.45e-5 SMART
CCP 569 622 5.56e-9 SMART
CCP 629 683 3.45e-14 SMART
CCP 690 743 1.82e-13 SMART
CCP 752 802 6.59e-1 SMART
CCP 808 861 1.04e-8 SMART
CCP 867 931 4.66e-11 SMART
CCP 936 989 3.9e-13 SMART
CCP 994 1048 1.4e-14 SMART
CCP 1053 1107 2.09e-13 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000148225
Predicted Effect probably benign
Transcript: ENSMUST00000192880
AA Change: I294N

PolyPhen 2 Score 0.175 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000141209
Gene: ENSMUSG00000026365
AA Change: I294N

DomainStartEndE-ValueType
signal peptide 1 36 N/A INTRINSIC
CCP 39 98 3.9e-10 SMART
CCP 103 159 1e-13 SMART
CCP 164 223 3.7e-17 SMART
CCP 228 280 3.1e-10 SMART
CCP 285 338 9.9e-10 SMART
CCP 343 403 3.2e-6 SMART
CCP 407 460 5.6e-13 SMART
CCP 467 521 6.7e-17 SMART
CCP 526 580 1e-15 SMART
CCP 587 641 3.8e-17 SMART
CCP 645 706 2.7e-2 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000192919
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.1%
  • 20x: 94.7%
Validation Efficiency 100% (42/42)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]
PHENOTYPE: Homozygous mutation of this gene results in markedly reduced serum C3, abnormal renal histology, spontaneous membranoproliferative glomerulonephritis (MPGN), hematuria, proteinuria, and increased mortality at 8 months of age. [provided by MGI curators]
Allele List at MGI

All alleles(2) : Targeted, knock-out(1) Targeted, other(1)

Other mutations in this stock
Total: 43 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca14 A G 7: 119,846,164 (GRCm39) D605G probably damaging Het
Clk3 A G 9: 57,669,078 (GRCm39) S49P probably damaging Het
Cmya5 A G 13: 93,229,403 (GRCm39) V1895A probably benign Het
Cmya5 T C 13: 93,229,547 (GRCm39) D1847G probably damaging Het
Cwf19l1 T C 19: 44,103,138 (GRCm39) D359G probably benign Het
Dag1 A T 9: 108,086,289 (GRCm39) M284K probably damaging Het
Ergic2 T C 6: 148,091,079 (GRCm39) Y211C probably damaging Het
Espnl A C 1: 91,272,421 (GRCm39) I594L probably benign Het
Fam193b A G 13: 55,690,603 (GRCm39) S226P probably damaging Het
Fat2 T A 11: 55,143,088 (GRCm39) I4254L probably benign Het
Fhdc1 T C 3: 84,371,631 (GRCm39) Y108C probably damaging Het
Fut9 G C 4: 25,620,619 (GRCm39) T65S probably benign Het
Gm9507 A T 10: 77,647,493 (GRCm39) probably benign Het
Gpt2 G A 8: 86,244,681 (GRCm39) E325K probably benign Het
Grap2 A T 15: 80,532,723 (GRCm39) N297Y probably damaging Het
Igfn1 A G 1: 135,891,609 (GRCm39) F2302S probably damaging Het
Ighv1-81 T A 12: 115,884,051 (GRCm39) I67F probably damaging Het
Kidins220 T A 12: 25,060,059 (GRCm39) probably null Het
Kti12 T A 4: 108,705,730 (GRCm39) S215T probably benign Het
Mc1r G A 8: 124,134,370 (GRCm39) G41D probably damaging Het
Mov10l1 A G 15: 88,878,105 (GRCm39) Y129C probably damaging Het
Mthfsd A T 8: 121,825,560 (GRCm39) L337Q probably damaging Het
Musk C T 4: 58,368,977 (GRCm39) A629V probably damaging Het
Nadsyn1 A T 7: 143,364,955 (GRCm39) M250K probably benign Het
Ncapd2 G A 6: 125,163,233 (GRCm39) H92Y probably benign Het
Or4e5 C T 14: 52,728,250 (GRCm39) R57Q probably benign Het
Or6y1 G A 1: 174,276,627 (GRCm39) C146Y probably damaging Het
Plec A T 15: 76,063,974 (GRCm39) V2100E probably damaging Het
Prss37 C A 6: 40,496,090 (GRCm39) probably benign Het
Sebox A T 11: 78,394,631 (GRCm39) E32V probably damaging Het
Senp7 C A 16: 55,944,257 (GRCm39) Q194K probably benign Het
Sfxn3 G A 19: 45,038,354 (GRCm39) probably null Het
Spart G A 3: 55,032,248 (GRCm39) E361K probably benign Het
Stn1 T C 19: 47,496,017 (GRCm39) T334A probably benign Het
Stoml1 A G 9: 58,164,017 (GRCm39) D112G probably damaging Het
Thap2 T A 10: 115,212,441 (GRCm39) D28V probably damaging Het
Ubap2 A T 4: 41,196,743 (GRCm39) N872K possibly damaging Het
Vezt A G 10: 93,806,141 (GRCm39) F757L probably damaging Het
Vmn1r204 T C 13: 22,740,573 (GRCm39) I68T probably damaging Het
Wnt10a A G 1: 74,842,613 (GRCm39) probably null Het
Yipf7 A G 5: 69,698,504 (GRCm39) M1T probably null Het
Zdhhc19 T C 16: 32,316,047 (GRCm39) F48S probably damaging Het
Zfp317 C T 9: 19,558,335 (GRCm39) T183I probably damaging Het
Other mutations in Cfh
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00969:Cfh APN 1 140,016,420 (GRCm39) missense probably damaging 1.00
IGL01124:Cfh APN 1 140,110,999 (GRCm39) missense probably benign 0.01
IGL01389:Cfh APN 1 140,082,377 (GRCm39) missense probably benign 0.44
IGL01455:Cfh APN 1 140,033,277 (GRCm39) missense possibly damaging 0.51
IGL01877:Cfh APN 1 140,028,567 (GRCm39) missense probably damaging 1.00
IGL02836:Cfh APN 1 140,030,137 (GRCm39) missense probably damaging 1.00
IGL02937:Cfh APN 1 140,033,180 (GRCm39) missense probably benign 0.19
IGL03039:Cfh APN 1 140,063,999 (GRCm39) missense possibly damaging 0.86
IGL03069:Cfh APN 1 140,026,793 (GRCm39) intron probably benign
IGL03192:Cfh APN 1 140,026,759 (GRCm39) missense possibly damaging 0.71
IGL03201:Cfh APN 1 140,030,557 (GRCm39) missense probably damaging 1.00
3-1:Cfh UTSW 1 140,090,863 (GRCm39) missense probably damaging 1.00
PIT4449001:Cfh UTSW 1 140,040,303 (GRCm39) missense probably damaging 1.00
R0257:Cfh UTSW 1 140,071,773 (GRCm39) missense probably benign 0.01
R0294:Cfh UTSW 1 140,110,999 (GRCm39) missense probably benign 0.01
R0571:Cfh UTSW 1 140,030,071 (GRCm39) splice site probably null
R0576:Cfh UTSW 1 140,064,553 (GRCm39) missense probably damaging 0.99
R0586:Cfh UTSW 1 140,110,920 (GRCm39) missense probably damaging 0.98
R0605:Cfh UTSW 1 140,030,096 (GRCm39) missense probably damaging 1.00
R0617:Cfh UTSW 1 140,028,621 (GRCm39) missense probably benign 0.01
R0725:Cfh UTSW 1 140,085,081 (GRCm39) splice site probably benign
R0853:Cfh UTSW 1 140,033,228 (GRCm39) missense probably damaging 1.00
R1430:Cfh UTSW 1 140,030,436 (GRCm39) splice site probably benign
R1500:Cfh UTSW 1 140,028,614 (GRCm39) missense probably damaging 1.00
R1533:Cfh UTSW 1 140,028,716 (GRCm39) missense possibly damaging 0.86
R1667:Cfh UTSW 1 140,033,261 (GRCm39) missense probably benign 0.01
R1695:Cfh UTSW 1 140,030,575 (GRCm39) missense probably damaging 0.98
R1728:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1729:Cfh UTSW 1 140,064,526 (GRCm39) missense probably benign 0.02
R1729:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1730:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1739:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1739:Cfh UTSW 1 140,064,526 (GRCm39) missense probably benign 0.02
R1756:Cfh UTSW 1 140,028,615 (GRCm39) missense probably damaging 1.00
R1762:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1762:Cfh UTSW 1 140,064,526 (GRCm39) missense probably benign 0.02
R1783:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1784:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1785:Cfh UTSW 1 140,075,435 (GRCm39) missense possibly damaging 0.55
R1785:Cfh UTSW 1 140,064,526 (GRCm39) missense probably benign 0.02
R1912:Cfh UTSW 1 140,063,879 (GRCm39) splice site probably null
R2273:Cfh UTSW 1 140,030,563 (GRCm39) missense probably damaging 1.00
R2288:Cfh UTSW 1 140,026,639 (GRCm39) missense possibly damaging 0.70
R3725:Cfh UTSW 1 140,014,234 (GRCm39) missense probably damaging 0.99
R3731:Cfh UTSW 1 140,047,708 (GRCm39) missense possibly damaging 0.71
R4060:Cfh UTSW 1 140,047,664 (GRCm39) missense possibly damaging 0.91
R4192:Cfh UTSW 1 140,030,454 (GRCm39) missense possibly damaging 0.50
R4226:Cfh UTSW 1 140,036,664 (GRCm39) missense probably damaging 1.00
R4425:Cfh UTSW 1 140,028,613 (GRCm39) nonsense probably null
R4431:Cfh UTSW 1 140,064,004 (GRCm39) missense probably damaging 1.00
R4712:Cfh UTSW 1 140,036,274 (GRCm39) missense probably damaging 1.00
R4755:Cfh UTSW 1 140,016,546 (GRCm39) missense probably damaging 1.00
R4792:Cfh UTSW 1 140,028,561 (GRCm39) nonsense probably null
R4831:Cfh UTSW 1 140,014,125 (GRCm39) missense probably benign
R5052:Cfh UTSW 1 140,071,782 (GRCm39) missense probably damaging 0.96
R5181:Cfh UTSW 1 140,075,384 (GRCm39) splice site probably benign
R5205:Cfh UTSW 1 140,071,708 (GRCm39) missense probably damaging 1.00
R5285:Cfh UTSW 1 140,028,636 (GRCm39) missense probably benign 0.21
R5366:Cfh UTSW 1 140,063,973 (GRCm39) missense probably damaging 1.00
R5776:Cfh UTSW 1 140,071,761 (GRCm39) missense possibly damaging 0.83
R5914:Cfh UTSW 1 140,063,967 (GRCm39) missense probably benign 0.39
R5948:Cfh UTSW 1 140,036,546 (GRCm39) missense probably damaging 0.96
R5979:Cfh UTSW 1 140,046,409 (GRCm39) missense possibly damaging 0.66
R6034:Cfh UTSW 1 140,090,869 (GRCm39) missense probably damaging 0.98
R6034:Cfh UTSW 1 140,090,869 (GRCm39) missense probably damaging 0.98
R6059:Cfh UTSW 1 140,046,428 (GRCm39) missense possibly damaging 0.92
R6198:Cfh UTSW 1 140,033,178 (GRCm39) missense probably damaging 1.00
R6306:Cfh UTSW 1 140,030,155 (GRCm39) missense probably damaging 1.00
R6523:Cfh UTSW 1 140,029,445 (GRCm39) missense possibly damaging 0.82
R6610:Cfh UTSW 1 140,029,486 (GRCm39) nonsense probably null
R6852:Cfh UTSW 1 140,075,487 (GRCm39) missense probably damaging 1.00
R6861:Cfh UTSW 1 140,028,621 (GRCm39) missense probably benign 0.07
R6862:Cfh UTSW 1 140,030,100 (GRCm39) missense probably damaging 1.00
R7065:Cfh UTSW 1 140,014,140 (GRCm39) missense probably damaging 0.99
R7191:Cfh UTSW 1 140,040,305 (GRCm39) missense probably benign 0.04
R7197:Cfh UTSW 1 140,016,505 (GRCm39) nonsense probably null
R7355:Cfh UTSW 1 140,064,553 (GRCm39) missense probably damaging 1.00
R7367:Cfh UTSW 1 140,014,259 (GRCm39) missense probably damaging 0.97
R7419:Cfh UTSW 1 140,033,204 (GRCm39) missense probably damaging 0.99
R7579:Cfh UTSW 1 140,036,328 (GRCm39) missense possibly damaging 0.53
R7586:Cfh UTSW 1 140,075,459 (GRCm39) missense probably damaging 0.99
R7985:Cfh UTSW 1 140,036,564 (GRCm39) missense probably damaging 1.00
R8119:Cfh UTSW 1 140,047,753 (GRCm39) missense possibly damaging 0.95
R8277:Cfh UTSW 1 140,029,347 (GRCm39) missense probably damaging 1.00
R8742:Cfh UTSW 1 140,029,390 (GRCm39) missense probably damaging 0.98
R8742:Cfh UTSW 1 140,064,469 (GRCm39) missense probably damaging 0.97
R8743:Cfh UTSW 1 140,046,323 (GRCm39) critical splice donor site probably null
R8874:Cfh UTSW 1 140,014,159 (GRCm39) missense probably damaging 1.00
R8909:Cfh UTSW 1 140,014,086 (GRCm39) missense possibly damaging 0.47
R8949:Cfh UTSW 1 140,026,705 (GRCm39) missense probably damaging 0.98
R9126:Cfh UTSW 1 140,014,111 (GRCm39) missense probably damaging 0.98
R9309:Cfh UTSW 1 140,082,249 (GRCm39) missense probably damaging 0.99
R9441:Cfh UTSW 1 140,030,149 (GRCm39) missense probably benign 0.08
R9502:Cfh UTSW 1 140,040,320 (GRCm39) missense possibly damaging 0.85
R9544:Cfh UTSW 1 140,036,266 (GRCm39) missense probably benign 0.14
R9559:Cfh UTSW 1 140,030,275 (GRCm39) missense probably benign 0.32
R9616:Cfh UTSW 1 140,030,254 (GRCm39) missense probably damaging 0.99
R9617:Cfh UTSW 1 140,090,718 (GRCm39) missense possibly damaging 0.53
R9733:Cfh UTSW 1 140,016,533 (GRCm39) missense probably damaging 1.00
R9748:Cfh UTSW 1 140,090,687 (GRCm39) critical splice donor site probably null
R9788:Cfh UTSW 1 140,036,499 (GRCm39) missense probably benign 0.01
T0975:Cfh UTSW 1 140,082,336 (GRCm39) missense probably benign 0.05
Z1088:Cfh UTSW 1 140,075,456 (GRCm39) missense possibly damaging 0.77
Z1088:Cfh UTSW 1 140,036,642 (GRCm39) missense probably benign 0.04
Z1177:Cfh UTSW 1 140,071,797 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TTCATCTGTGCATAGTGAATAGGC -3'
(R):5'- GAGAGGAAACCTGACTGATTCG -3'

Sequencing Primer
(F):5'- TAGGCTAATTTCAGGATCATGGAG -3'
(R):5'- ACCTGACTGATTCGTTAAATATGTG -3'
Posted On 2018-07-24