Mutagenetix > Incidental Mutation

Incidental Mutation 'R6652:Vmn1r204'

528107

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r204

Ensembl Gene

ENSMUSG00000094637

Gene Name

vomeronasal 1 receptor 204

Synonyms

Gm11301

MMRRC Submission

044773-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R6652 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

22740371-22741279 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22740573 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 68 (I68T)

Ref Sequence

ENSEMBL: ENSMUSP00000154519 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091728] [ENSMUST00000228195]

AlphaFold

I7HIK1

Predicted Effect

probably damaging
Transcript: ENSMUST00000091728
AA Change: I68T

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000089321
Gene: ENSMUSG00000094637
AA Change: I68T

Domain	Start	End	E-Value	Type
Pfam:TAS2R	3	293	8.4e-8	PFAM
Pfam:V1R	35	297	1.2e-40	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000228195
AA Change: I68T

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.7%

Validation Efficiency

100% (42/42)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	A	G	7: 119,846,164 (GRCm39)	D605G	probably damaging	Het
Cfh	A	T	1: 140,071,806 (GRCm39)	I294N	probably benign	Het
Clk3	A	G	9: 57,669,078 (GRCm39)	S49P	probably damaging	Het
Cmya5	A	G	13: 93,229,403 (GRCm39)	V1895A	probably benign	Het
Cmya5	T	C	13: 93,229,547 (GRCm39)	D1847G	probably damaging	Het
Cwf19l1	T	C	19: 44,103,138 (GRCm39)	D359G	probably benign	Het
Dag1	A	T	9: 108,086,289 (GRCm39)	M284K	probably damaging	Het
Ergic2	T	C	6: 148,091,079 (GRCm39)	Y211C	probably damaging	Het
Espnl	A	C	1: 91,272,421 (GRCm39)	I594L	probably benign	Het
Fam193b	A	G	13: 55,690,603 (GRCm39)	S226P	probably damaging	Het
Fat2	T	A	11: 55,143,088 (GRCm39)	I4254L	probably benign	Het
Fhdc1	T	C	3: 84,371,631 (GRCm39)	Y108C	probably damaging	Het
Fut9	G	C	4: 25,620,619 (GRCm39)	T65S	probably benign	Het
Gm9507	A	T	10: 77,647,493 (GRCm39)		probably benign	Het
Gpt2	G	A	8: 86,244,681 (GRCm39)	E325K	probably benign	Het
Grap2	A	T	15: 80,532,723 (GRCm39)	N297Y	probably damaging	Het
Igfn1	A	G	1: 135,891,609 (GRCm39)	F2302S	probably damaging	Het
Ighv1-81	T	A	12: 115,884,051 (GRCm39)	I67F	probably damaging	Het
Kidins220	T	A	12: 25,060,059 (GRCm39)		probably null	Het
Kti12	T	A	4: 108,705,730 (GRCm39)	S215T	probably benign	Het
Mc1r	G	A	8: 124,134,370 (GRCm39)	G41D	probably damaging	Het
Mov10l1	A	G	15: 88,878,105 (GRCm39)	Y129C	probably damaging	Het
Mthfsd	A	T	8: 121,825,560 (GRCm39)	L337Q	probably damaging	Het
Musk	C	T	4: 58,368,977 (GRCm39)	A629V	probably damaging	Het
Nadsyn1	A	T	7: 143,364,955 (GRCm39)	M250K	probably benign	Het
Ncapd2	G	A	6: 125,163,233 (GRCm39)	H92Y	probably benign	Het
Or4e5	C	T	14: 52,728,250 (GRCm39)	R57Q	probably benign	Het
Or6y1	G	A	1: 174,276,627 (GRCm39)	C146Y	probably damaging	Het
Plec	A	T	15: 76,063,974 (GRCm39)	V2100E	probably damaging	Het
Prss37	C	A	6: 40,496,090 (GRCm39)		probably benign	Het
Sebox	A	T	11: 78,394,631 (GRCm39)	E32V	probably damaging	Het
Senp7	C	A	16: 55,944,257 (GRCm39)	Q194K	probably benign	Het
Sfxn3	G	A	19: 45,038,354 (GRCm39)		probably null	Het
Spart	G	A	3: 55,032,248 (GRCm39)	E361K	probably benign	Het
Stn1	T	C	19: 47,496,017 (GRCm39)	T334A	probably benign	Het
Stoml1	A	G	9: 58,164,017 (GRCm39)	D112G	probably damaging	Het
Thap2	T	A	10: 115,212,441 (GRCm39)	D28V	probably damaging	Het
Ubap2	A	T	4: 41,196,743 (GRCm39)	N872K	possibly damaging	Het
Vezt	A	G	10: 93,806,141 (GRCm39)	F757L	probably damaging	Het
Wnt10a	A	G	1: 74,842,613 (GRCm39)		probably null	Het
Yipf7	A	G	5: 69,698,504 (GRCm39)	M1T	probably null	Het
Zdhhc19	T	C	16: 32,316,047 (GRCm39)	F48S	probably damaging	Het
Zfp317	C	T	9: 19,558,335 (GRCm39)	T183I	probably damaging	Het

Other mutations in Vmn1r204

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Vmn1r204	APN	13	22,741,117 (GRCm39)	nonsense	probably null
IGL01349:Vmn1r204	APN	13	22,740,504 (GRCm39)	missense	probably damaging	0.99
IGL02653:Vmn1r204	APN	13	22,740,800 (GRCm39)	missense	probably benign	0.34
R0029:Vmn1r204	UTSW	13	22,740,588 (GRCm39)	missense	probably benign	0.12
R0078:Vmn1r204	UTSW	13	22,740,379 (GRCm39)	missense	probably benign	0.01
R0562:Vmn1r204	UTSW	13	22,740,848 (GRCm39)	missense	probably benign	0.00
R1124:Vmn1r204	UTSW	13	22,741,209 (GRCm39)	missense	possibly damaging	0.76
R1521:Vmn1r204	UTSW	13	22,741,248 (GRCm39)	missense	probably benign	0.30
R1544:Vmn1r204	UTSW	13	22,740,465 (GRCm39)	missense	probably benign	0.13
R2238:Vmn1r204	UTSW	13	22,740,993 (GRCm39)	missense	probably benign	0.01
R2418:Vmn1r204	UTSW	13	22,740,420 (GRCm39)	missense	probably damaging	1.00
R2419:Vmn1r204	UTSW	13	22,740,420 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn1r204	UTSW	13	22,741,014 (GRCm39)	missense	probably benign	0.02
R4675:Vmn1r204	UTSW	13	22,740,962 (GRCm39)	missense	probably damaging	1.00
R4782:Vmn1r204	UTSW	13	22,740,867 (GRCm39)	missense	probably benign	0.03
R4985:Vmn1r204	UTSW	13	22,741,230 (GRCm39)	missense	probably damaging	1.00
R5219:Vmn1r204	UTSW	13	22,741,069 (GRCm39)	missense	probably damaging	1.00
R5268:Vmn1r204	UTSW	13	22,740,912 (GRCm39)	missense	probably damaging	1.00
R6943:Vmn1r204	UTSW	13	22,740,474 (GRCm39)	missense	probably benign	0.00
R7264:Vmn1r204	UTSW	13	22,741,167 (GRCm39)	missense	probably benign	0.23
R7299:Vmn1r204	UTSW	13	22,740,975 (GRCm39)	missense	probably damaging	1.00
R7301:Vmn1r204	UTSW	13	22,740,975 (GRCm39)	missense	probably damaging	1.00
R7593:Vmn1r204	UTSW	13	22,740,754 (GRCm39)	nonsense	probably null
R7872:Vmn1r204	UTSW	13	22,740,404 (GRCm39)	missense	probably benign	0.00
R8495:Vmn1r204	UTSW	13	22,740,879 (GRCm39)	missense	probably damaging	1.00
R9110:Vmn1r204	UTSW	13	22,740,564 (GRCm39)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- GGCTTTCCTAGTTCTGAGCATG -3'
(R):5'- CATGCCGTCTGTGGTTTGAAC -3'

Sequencing Primer
(F):5'- ATTTGGAGTGACCTCATCGC -3'
(R):5'- GTGGTTTGAACTTTCTCCACAG -3'

Posted On

2018-07-24