Mutagenetix > Incidental Mutation

Incidental Mutation 'R6734:Prune2'

528371

Institutional Source

Beutler Lab

Gene Symbol

Prune2

Ensembl Gene

ENSMUSG00000039126

Gene Name

prune homolog 2

Synonyms

A230083H22Rik, 6330414G02Rik, A330102H22Rik

MMRRC Submission

044852-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6734 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

16956118-17223932 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 17003733 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Valine at position 85 (F85V)

Ref Sequence

ENSEMBL: ENSMUSP00000084977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087689]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000087689
AA Change: F85V

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000084977
Gene: ENSMUSG00000039126
AA Change: F85V

Domain	Start	End	E-Value	Type
DHHA2	208	351	8.32e-17	SMART
low complexity region	433	445	N/A	INTRINSIC
low complexity region	476	488	N/A	INTRINSIC
low complexity region	547	553	N/A	INTRINSIC
low complexity region	962	975	N/A	INTRINSIC
low complexity region	1071	1082	N/A	INTRINSIC
low complexity region	1368	1378	N/A	INTRINSIC
low complexity region	1533	1545	N/A	INTRINSIC
low complexity region	1668	1685	N/A	INTRINSIC
low complexity region	1740	1751	N/A	INTRINSIC
low complexity region	2162	2175	N/A	INTRINSIC
low complexity region	2222	2233	N/A	INTRINSIC
low complexity region	2591	2606	N/A	INTRINSIC
low complexity region	2731	2744	N/A	INTRINSIC
SEC14	2882	3037	2.08e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000224145

Meta Mutation Damage Score

0.0970

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Allele List at MGI

All alleles(160) : Gene trapped(160)

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot11	C	T	4: 106,760,130 (GRCm38)	G240R	probably damaging	Het
Ano6	A	G	15: 95,949,536 (GRCm38)	K554R	probably damaging	Het
Arhgef10	A	T	8: 14,975,053 (GRCm38)	I703F	probably damaging	Het
Bbs1	T	C	19: 4,903,896 (GRCm38)	S80G	probably benign	Het
Bhlhe40	TG	TGG	6: 108,664,857 (GRCm38)	254	probably null	Het
Camk1	C	A	6: 113,334,384 (GRCm38)	R352L	probably benign	Het
Cand1	G	A	10: 119,211,992 (GRCm38)	P531L	possibly damaging	Het
Cnot2	G	A	10: 116,498,153 (GRCm38)	P371S	possibly damaging	Het
Col24a1	C	A	3: 145,508,674 (GRCm38)	P1384Q	probably benign	Het
Csmd2	C	A	4: 128,463,813 (GRCm38)	T1689K	probably benign	Het
Cyp26a1	T	A	19: 37,701,212 (GRCm38)	L452H	probably damaging	Het
Dcpp2	T	C	17: 23,900,571 (GRCm38)	Y120H	probably damaging	Het
Dennd6a	G	T	14: 26,608,619 (GRCm38)	R115L	possibly damaging	Het
Eml2	T	A	7: 19,200,507 (GRCm38)	V377E	probably benign	Het
Fam135b	T	C	15: 71,462,780 (GRCm38)	E855G	probably benign	Het
Fam149a	A	T	8: 45,381,441 (GRCm38)	I107K	probably benign	Het
Fam227b	A	T	2: 126,126,976 (GRCm38)	Y59*	probably null	Het
Fbn2	T	C	18: 58,035,960 (GRCm38)	E2249G	probably damaging	Het
Flrt1	T	C	19: 7,096,159 (GRCm38)	D341G	possibly damaging	Het
Galnt9	G	A	5: 110,620,599 (GRCm38)	R587H	probably damaging	Het
Gm10334	T	C	6: 41,445,387 (GRCm38)	Y28C	probably damaging	Het
Gsdma2	A	G	11: 98,649,590 (GRCm38)	T112A	possibly damaging	Het
Klhl24	T	C	16: 20,107,529 (GRCm38)	V269A	probably damaging	Het
Lcorl	A	G	5: 45,733,497 (GRCm38)	S505P	probably damaging	Het
Liph	A	G	16: 21,983,957 (GRCm38)	S121P	probably damaging	Het
Lrp4	G	A	2: 91,485,897 (GRCm38)	V787M	possibly damaging	Het
Lrrd1	A	C	5: 3,850,226 (GRCm38)	D177A	possibly damaging	Het
Mbd1	G	A	18: 74,276,043 (GRCm38)	R399H	probably damaging	Het
Naa25	C	T	5: 121,438,825 (GRCm38)	T879M	possibly damaging	Het
Naa35	T	G	13: 59,608,191 (GRCm38)	L147R	possibly damaging	Het
Ninl	C	A	2: 150,945,083 (GRCm38)		probably null	Het
Nrap	T	C	19: 56,345,509 (GRCm38)	D972G	probably damaging	Het
Olfr827	A	G	10: 130,210,257 (GRCm38)	F291S	probably benign	Het
Pdzd2	T	C	15: 12,592,465 (GRCm38)	E31G	probably damaging	Het
Plec	C	T	15: 76,194,403 (GRCm38)	E41K	probably damaging	Het
Plxnb1	A	T	9: 109,108,920 (GRCm38)	K1245*	probably null	Het
Ppfia1	T	C	7: 144,479,053 (GRCm38)	T1263A	probably damaging	Het
Prex2	G	A	1: 11,080,059 (GRCm38)	V152I	probably damaging	Het
Prpf40b	C	T	15: 99,314,903 (GRCm38)	R627W	probably damaging	Het
Rrp1b	T	G	17: 32,055,304 (GRCm38)		probably benign	Het
Sec62	A	G	3: 30,810,460 (GRCm38)	T158A	probably benign	Het
Sema6a	G	A	18: 47,279,169 (GRCm38)	T526I	probably benign	Het
Shank1	G	T	7: 44,353,686 (GRCm38)	A1610S	probably benign	Het
Slc24a4	T	C	12: 102,219,000 (GRCm38)	V123A	probably damaging	Het
Stk11ip	T	C	1: 75,532,369 (GRCm38)	V714A	probably benign	Het
Tas1r3	T	C	4: 155,860,800 (GRCm38)	T655A	probably damaging	Het
Tns2	G	T	15: 102,103,116 (GRCm38)	L10F	probably damaging	Het
Trmt10c	A	G	16: 56,034,126 (GRCm38)	V382A	probably benign	Het
Unc45a	G	C	7: 80,336,998 (GRCm38)	T149R	probably damaging	Het
Zhx3	T	G	2: 160,781,720 (GRCm38)	I176L	probably damaging	Het
Zscan12	T	A	13: 21,368,796 (GRCm38)	C263*	probably null	Het

Other mutations in Prune2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00234:Prune2	APN	19	17,168,344 (GRCm38)	critical splice donor site	probably null
IGL00848:Prune2	APN	19	17,119,118 (GRCm38)	missense	probably damaging	1.00
IGL00862:Prune2	APN	19	17,119,349 (GRCm38)	missense	probably benign	0.41
IGL00915:Prune2	APN	19	17,016,253 (GRCm38)	missense	probably damaging	1.00
IGL01084:Prune2	APN	19	17,118,209 (GRCm38)	missense	probably benign	0.19
IGL01109:Prune2	APN	19	17,123,879 (GRCm38)	missense	probably benign	0.03
IGL01372:Prune2	APN	19	17,125,069 (GRCm38)	missense	probably damaging	1.00
IGL01650:Prune2	APN	19	17,168,292 (GRCm38)	missense	possibly damaging	0.95
IGL01752:Prune2	APN	19	17,123,903 (GRCm38)	missense	possibly damaging	0.50
IGL01812:Prune2	APN	19	17,003,777 (GRCm38)	missense	possibly damaging	0.50
IGL01902:Prune2	APN	19	17,118,638 (GRCm38)	missense	probably benign	0.00
IGL02195:Prune2	APN	19	17,119,557 (GRCm38)	missense	probably benign	0.00
IGL02502:Prune2	APN	19	17,123,881 (GRCm38)	missense	probably benign	0.00
IGL02569:Prune2	APN	19	17,178,859 (GRCm38)	missense	probably damaging	0.99
IGL02693:Prune2	APN	19	17,124,491 (GRCm38)	missense	probably benign	0.03
IGL02737:Prune2	APN	19	17,193,411 (GRCm38)	nonsense	probably null
IGL02794:Prune2	APN	19	17,119,361 (GRCm38)	missense	probably benign	0.19
IGL02985:Prune2	APN	19	17,016,359 (GRCm38)	critical splice donor site	probably null
IGL03349:Prune2	APN	19	17,123,346 (GRCm38)	missense	probably damaging	1.00
3-1:Prune2	UTSW	19	17,125,282 (GRCm38)	missense	probably benign	0.00
R0060:Prune2	UTSW	19	17,003,733 (GRCm38)	missense	probably damaging	1.00
R0098:Prune2	UTSW	19	17,123,903 (GRCm38)	missense	possibly damaging	0.50
R0098:Prune2	UTSW	19	17,123,903 (GRCm38)	missense	possibly damaging	0.50
R0165:Prune2	UTSW	19	17,122,610 (GRCm38)	missense	probably benign	0.00
R0277:Prune2	UTSW	19	17,121,389 (GRCm38)	missense	probably damaging	0.99
R0321:Prune2	UTSW	19	17,122,454 (GRCm38)	missense	probably benign	0.39
R0321:Prune2	UTSW	19	17,120,927 (GRCm38)	missense	possibly damaging	0.78
R0374:Prune2	UTSW	19	17,120,910 (GRCm38)	missense	probably benign	0.00
R0380:Prune2	UTSW	19	17,124,007 (GRCm38)	missense	probably damaging	1.00
R0396:Prune2	UTSW	19	17,123,080 (GRCm38)	missense	probably benign	0.35
R0408:Prune2	UTSW	19	17,122,310 (GRCm38)	missense	probably benign	0.00
R0421:Prune2	UTSW	19	17,123,311 (GRCm38)	missense	probably benign	0.02
R0480:Prune2	UTSW	19	17,006,792 (GRCm38)	splice site	probably benign
R0531:Prune2	UTSW	19	17,006,753 (GRCm38)	missense	probably damaging	1.00
R0546:Prune2	UTSW	19	17,020,666 (GRCm38)	splice site	probably benign
R0554:Prune2	UTSW	19	17,125,218 (GRCm38)	nonsense	probably null
R0659:Prune2	UTSW	19	17,122,835 (GRCm38)	missense	probably damaging	1.00
R0699:Prune2	UTSW	19	17,123,955 (GRCm38)	missense	probably damaging	1.00
R0781:Prune2	UTSW	19	17,125,222 (GRCm38)	missense	probably benign
R1110:Prune2	UTSW	19	17,125,222 (GRCm38)	missense	probably benign
R1178:Prune2	UTSW	19	17,123,105 (GRCm38)	missense	probably benign	0.22
R1181:Prune2	UTSW	19	17,123,105 (GRCm38)	missense	probably benign	0.22
R1337:Prune2	UTSW	19	17,119,607 (GRCm38)	missense	possibly damaging	0.70
R1356:Prune2	UTSW	19	17,212,317 (GRCm38)	missense	probably benign	0.40
R1385:Prune2	UTSW	19	17,124,948 (GRCm38)	missense	possibly damaging	0.50
R1659:Prune2	UTSW	19	17,120,651 (GRCm38)	missense	possibly damaging	0.59
R1738:Prune2	UTSW	19	17,125,010 (GRCm38)	missense	probably benign	0.01
R1756:Prune2	UTSW	19	17,123,704 (GRCm38)	missense	probably benign	0.01
R1765:Prune2	UTSW	19	17,125,598 (GRCm38)	missense	probably damaging	1.00
R1782:Prune2	UTSW	19	17,122,173 (GRCm38)	missense	probably benign	0.00
R1817:Prune2	UTSW	19	17,122,081 (GRCm38)	missense	probably benign	0.00
R1838:Prune2	UTSW	19	17,199,878 (GRCm38)	missense	probably damaging	1.00
R1851:Prune2	UTSW	19	17,199,139 (GRCm38)	missense	probably damaging	1.00
R1852:Prune2	UTSW	19	17,199,139 (GRCm38)	missense	probably damaging	1.00
R1866:Prune2	UTSW	19	17,123,492 (GRCm38)	missense	probably damaging	1.00
R1911:Prune2	UTSW	19	17,113,674 (GRCm38)	missense	probably benign	0.02
R1983:Prune2	UTSW	19	17,020,642 (GRCm38)	missense	probably damaging	0.97
R2014:Prune2	UTSW	19	17,120,523 (GRCm38)	missense	probably damaging	1.00
R2066:Prune2	UTSW	19	17,120,678 (GRCm38)	missense	possibly damaging	0.57
R2088:Prune2	UTSW	19	17,119,745 (GRCm38)	missense	possibly damaging	0.95
R2111:Prune2	UTSW	19	17,208,238 (GRCm38)	missense	probably damaging	1.00
R2128:Prune2	UTSW	19	17,122,422 (GRCm38)	missense	probably benign	0.00
R2165:Prune2	UTSW	19	17,120,182 (GRCm38)	missense	probably benign	0.19
R2241:Prune2	UTSW	19	17,123,092 (GRCm38)	missense	probably damaging	0.96
R2278:Prune2	UTSW	19	17,118,555 (GRCm38)	missense	possibly damaging	0.93
R2504:Prune2	UTSW	19	17,000,036 (GRCm38)	missense	probably damaging	1.00
R2508:Prune2	UTSW	19	17,122,622 (GRCm38)	missense	probably benign	0.43
R3055:Prune2	UTSW	19	17,125,043 (GRCm38)	missense	probably damaging	0.98
R3086:Prune2	UTSW	19	17,121,413 (GRCm38)	missense	possibly damaging	0.75
R3104:Prune2	UTSW	19	17,119,156 (GRCm38)	missense	probably damaging	1.00
R3105:Prune2	UTSW	19	17,119,156 (GRCm38)	missense	probably damaging	1.00
R3547:Prune2	UTSW	19	17,124,348 (GRCm38)	missense	probably damaging	0.96
R3702:Prune2	UTSW	19	17,178,871 (GRCm38)	missense	probably damaging	1.00
R3753:Prune2	UTSW	19	17,125,454 (GRCm38)	missense	probably benign	0.38
R3933:Prune2	UTSW	19	17,123,954 (GRCm38)	missense	probably damaging	1.00
R3935:Prune2	UTSW	19	17,199,786 (GRCm38)	missense	probably damaging	1.00
R4022:Prune2	UTSW	19	17,000,020 (GRCm38)	missense	probably damaging	1.00
R4042:Prune2	UTSW	19	17,003,826 (GRCm38)	critical splice donor site	probably null
R4164:Prune2	UTSW	19	17,003,734 (GRCm38)	missense	possibly damaging	0.87
R4453:Prune2	UTSW	19	17,121,910 (GRCm38)	missense	probably benign	0.00
R4642:Prune2	UTSW	19	17,020,655 (GRCm38)	critical splice donor site	probably null
R4661:Prune2	UTSW	19	17,000,023 (GRCm38)	missense	probably damaging	1.00
R4666:Prune2	UTSW	19	17,120,188 (GRCm38)	nonsense	probably null
R4823:Prune2	UTSW	19	17,120,504 (GRCm38)	missense	probably damaging	1.00
R4897:Prune2	UTSW	19	17,121,855 (GRCm38)	missense	probably benign	0.03
R4922:Prune2	UTSW	19	17,122,752 (GRCm38)	missense	probably benign	0.00
R4962:Prune2	UTSW	19	17,122,273 (GRCm38)	missense	probably benign	0.11
R5026:Prune2	UTSW	19	17,199,142 (GRCm38)	missense	probably damaging	1.00
R5042:Prune2	UTSW	19	17,119,797 (GRCm38)	missense	possibly damaging	0.94
R5124:Prune2	UTSW	19	17,199,910 (GRCm38)	missense	probably damaging	1.00
R5133:Prune2	UTSW	19	17,003,631 (GRCm38)	missense	probably damaging	1.00
R5184:Prune2	UTSW	19	17,216,357 (GRCm38)	missense	possibly damaging	0.95
R5234:Prune2	UTSW	19	17,118,668 (GRCm38)	missense	probably damaging	1.00
R5339:Prune2	UTSW	19	17,120,872 (GRCm38)	missense	probably damaging	1.00
R5363:Prune2	UTSW	19	17,118,266 (GRCm38)	missense	probably damaging	1.00
R5382:Prune2	UTSW	19	17,003,659 (GRCm38)	missense	probably damaging	1.00
R5436:Prune2	UTSW	19	17,020,643 (GRCm38)	missense	probably damaging	1.00
R5480:Prune2	UTSW	19	17,120,947 (GRCm38)	missense	possibly damaging	0.66
R5635:Prune2	UTSW	19	17,118,209 (GRCm38)	missense	probably benign	0.19
R5678:Prune2	UTSW	19	17,118,668 (GRCm38)	missense	probably damaging	1.00
R5814:Prune2	UTSW	19	17,016,361 (GRCm38)	splice site	probably null
R5894:Prune2	UTSW	19	17,121,391 (GRCm38)	missense	possibly damaging	0.88
R6011:Prune2	UTSW	19	17,118,716 (GRCm38)	missense	probably benign	0.35
R6207:Prune2	UTSW	19	17,118,116 (GRCm38)	missense	probably damaging	1.00
R6218:Prune2	UTSW	19	17,121,562 (GRCm38)	missense	probably benign	0.00
R6573:Prune2	UTSW	19	17,121,157 (GRCm38)	missense	probably damaging	1.00
R6573:Prune2	UTSW	19	17,121,158 (GRCm38)	missense	possibly damaging	0.61
R6805:Prune2	UTSW	19	17,120,590 (GRCm38)	missense	probably benign
R6837:Prune2	UTSW	19	17,178,928 (GRCm38)	missense	probably damaging	1.00
R6850:Prune2	UTSW	19	17,122,188 (GRCm38)	missense	probably benign	0.00
R6858:Prune2	UTSW	19	17,118,106 (GRCm38)	missense	possibly damaging	0.70
R6874:Prune2	UTSW	19	17,123,228 (GRCm38)	missense	probably damaging	1.00
R6954:Prune2	UTSW	19	17,000,021 (GRCm38)	missense	probably damaging	1.00
R7098:Prune2	UTSW	19	17,120,602 (GRCm38)	missense	probably benign	0.39
R7102:Prune2	UTSW	19	17,121,213 (GRCm38)	missense	probably benign	0.24
R7246:Prune2	UTSW	19	17,121,368 (GRCm38)	missense	probably damaging	0.99
R7284:Prune2	UTSW	19	17,119,886 (GRCm38)	missense	probably damaging	1.00
R7295:Prune2	UTSW	19	17,119,897 (GRCm38)	missense	probably benign	0.01
R7371:Prune2	UTSW	19	17,119,370 (GRCm38)	missense	probably benign	0.02
R7651:Prune2	UTSW	19	17,120,408 (GRCm38)	missense	probably damaging	1.00
R7830:Prune2	UTSW	19	17,122,674 (GRCm38)	missense	probably benign	0.21
R7872:Prune2	UTSW	19	17,119,434 (GRCm38)	missense	probably benign	0.05
R7881:Prune2	UTSW	19	17,123,029 (GRCm38)	missense	possibly damaging	0.50
R7966:Prune2	UTSW	19	17,178,859 (GRCm38)	missense	probably damaging	0.99
R7969:Prune2	UTSW	19	17,201,670 (GRCm38)	missense	probably damaging	0.98
R8092:Prune2	UTSW	19	17,119,993 (GRCm38)	missense	probably damaging	1.00
R8110:Prune2	UTSW	19	17,120,719 (GRCm38)	missense	probably benign	0.22
R8115:Prune2	UTSW	19	17,123,924 (GRCm38)	missense	probably benign	0.02
R8129:Prune2	UTSW	19	17,118,836 (GRCm38)	missense	probably benign	0.01
R8169:Prune2	UTSW	19	17,125,091 (GRCm38)	missense	probably benign	0.10
R8171:Prune2	UTSW	19	17,120,518 (GRCm38)	missense	probably damaging	1.00
R8176:Prune2	UTSW	19	17,118,292 (GRCm38)	missense	probably damaging	1.00
R8200:Prune2	UTSW	19	17,124,973 (GRCm38)	missense	probably benign	0.01
R8217:Prune2	UTSW	19	17,120,116 (GRCm38)	missense	probably benign	0.01
R8258:Prune2	UTSW	19	17,212,308 (GRCm38)	missense	unknown
R8259:Prune2	UTSW	19	17,212,308 (GRCm38)	missense	unknown
R8289:Prune2	UTSW	19	17,123,009 (GRCm38)	missense	probably benign	0.43
R8329:Prune2	UTSW	19	17,121,265 (GRCm38)	missense	probably benign	0.02
R8342:Prune2	UTSW	19	17,125,663 (GRCm38)	missense	probably benign	0.01
R8558:Prune2	UTSW	19	17,122,238 (GRCm38)	missense	probably damaging	0.98
R8732:Prune2	UTSW	19	17,120,405 (GRCm38)	missense	probably damaging	1.00
R8743:Prune2	UTSW	19	17,119,556 (GRCm38)	missense	probably benign	0.22
R8769:Prune2	UTSW	19	17,123,078 (GRCm38)	missense	probably damaging	0.96
R8862:Prune2	UTSW	19	17,120,146 (GRCm38)	missense	probably benign	0.04
R8936:Prune2	UTSW	19	17,121,835 (GRCm38)	missense	probably benign	0.24
R9040:Prune2	UTSW	19	17,120,627 (GRCm38)	missense	probably damaging	1.00
R9084:Prune2	UTSW	19	17,120,377 (GRCm38)	missense	probably damaging	1.00
R9224:Prune2	UTSW	19	17,120,029 (GRCm38)	missense	probably damaging	1.00
R9273:Prune2	UTSW	19	17,118,326 (GRCm38)	missense	possibly damaging	0.74
R9275:Prune2	UTSW	19	17,123,780 (GRCm38)	missense	probably benign	0.06
R9278:Prune2	UTSW	19	17,123,780 (GRCm38)	missense	probably benign	0.06
R9290:Prune2	UTSW	19	17,168,327 (GRCm38)	missense	probably benign	0.41
R9305:Prune2	UTSW	19	17,120,261 (GRCm38)	missense	probably benign	0.14
R9317:Prune2	UTSW	19	17,121,670 (GRCm38)	missense	probably benign	0.00
R9354:Prune2	UTSW	19	17,122,622 (GRCm38)	missense	probably benign	0.43
R9373:Prune2	UTSW	19	17,122,138 (GRCm38)	missense	probably benign
R9394:Prune2	UTSW	19	17,003,689 (GRCm38)	missense	probably damaging	1.00
R9405:Prune2	UTSW	19	17,216,344 (GRCm38)	missense	probably damaging	0.99
R9476:Prune2	UTSW	19	17,119,342 (GRCm38)	missense	possibly damaging	0.64
R9532:Prune2	UTSW	19	17,122,430 (GRCm38)	missense	probably benign	0.00
X0019:Prune2	UTSW	19	17,121,517 (GRCm38)	missense	probably benign	0.16
X0028:Prune2	UTSW	19	17,122,885 (GRCm38)	missense	probably damaging	1.00
X0064:Prune2	UTSW	19	17,122,375 (GRCm38)	missense	probably damaging	1.00
X0066:Prune2	UTSW	19	17,118,790 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAGCTGGAGGAAAACTGCTG -3'
(R):5'- AGAACTGGTGTAGCCCAAATATTCTC -3'

Sequencing Primer
(F):5'- CTGGAGGAAAACTGCTGTTTGC -3'
(R):5'- CTTCCCTCAGGACAAATG -3'

Posted On

2018-07-24