Mutagenetix > Incidental Mutation

Incidental Mutation 'R6736:Smg1'

528419

Institutional Source

Beutler Lab

Gene Symbol

Smg1

Ensembl Gene

ENSMUSG00000030655

Gene Name

SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)

Synonyms

2610207I05Rik, 5430435M13Rik, C130002K18Rik

MMRRC Submission

044854-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6736 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

118131308-118243670 bp(-) (GRCm38)

Type of Mutation

utr 3 prime

DNA Base Change (assembly)

A to G at 118157166 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032891]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000032891
AA Change: S2323P

SMART Domains

Protein: ENSMUSP00000032891
Gene: ENSMUSG00000030655
AA Change: S2323P

Domain	Start	End	E-Value	Type
low complexity region	42	55	N/A	INTRINSIC
SCOP:d1gw5a_	147	621	7e-7	SMART
Pfam:SMG1	629	1240	9.8e-249	PFAM
low complexity region	1540	1551	N/A	INTRINSIC
SCOP:d1gw5a_	1680	1942	8e-3	SMART
low complexity region	2125	2141	N/A	INTRINSIC
PI3Kc	2149	2493	7.93e-50	SMART
low complexity region	2759	2770	N/A	INTRINSIC
low complexity region	3425	3442	N/A	INTRINSIC
FATC	3626	3658	8.66e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000083940

Predicted Effect

probably benign
Transcript: ENSMUST00000179331

SMART Domains

Protein: ENSMUSP00000137592
Gene: ENSMUSG00000030655

Domain	Start	End	E-Value	Type
low complexity region	18	31	N/A	INTRINSIC
SCOP:d1gw5a_	71	545	1e-6	SMART
low complexity region	602	612	N/A	INTRINSIC
low complexity region	631	646	N/A	INTRINSIC
low complexity region	698	718	N/A	INTRINSIC
low complexity region	898	915	N/A	INTRINSIC
low complexity region	1135	1147	N/A	INTRINSIC
low complexity region	1464	1475	N/A	INTRINSIC
low complexity region	2049	2065	N/A	INTRINSIC
PI3Kc	2073	2417	7.93e-50	SMART
low complexity region	2683	2694	N/A	INTRINSIC
low complexity region	3349	3366	N/A	INTRINSIC
FATC	3550	3582	8.66e-12	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000208025

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]
PHENOTYPE: Mice homozygous for a gene trap allele exhibit early embryonic lethality. Mice heteroygous for a gene trap allele exhibit abnormal tooth development, chronic inflammation, increased body weight, increased incidence of tumor formation and premature death. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 102 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010I14Rik	T	C	17: 8,992,268 (GRCm38)	I83T	probably benign	Het
Aadacl4	T	G	4: 144,623,339 (GRCm38)	S389A	possibly damaging	Het
Abca13	A	T	11: 9,465,058 (GRCm38)	S4042C	probably damaging	Het
Acaca	G	A	11: 84,238,838 (GRCm38)	V340I	probably benign	Het
Acox3	T	A	5: 35,588,854 (GRCm38)		probably null	Het
Acsl6	A	C	11: 54,325,166 (GRCm38)	E124A	probably damaging	Het
Adamtsl1	T	A	4: 86,342,247 (GRCm38)	H898Q	probably damaging	Het
Agl	A	G	3: 116,781,680 (GRCm38)	S603P	probably damaging	Het
Apobec1	A	T	6: 122,581,675 (GRCm38)	M31K	probably null	Het
Armc4	C	A	18: 7,223,586 (GRCm38)	V486F	probably damaging	Het
Astn1	T	C	1: 158,511,148 (GRCm38)		probably null	Het
Atp8a1	G	T	5: 67,667,617 (GRCm38)	D790E	probably damaging	Het
Bahd1	T	G	2: 118,915,975 (GRCm38)	M25R	possibly damaging	Het
BC034090	A	T	1: 155,241,930 (GRCm38)	N147K	possibly damaging	Het
Bfsp2	G	T	9: 103,480,204 (GRCm38)	A8E	possibly damaging	Het
Brwd1	A	T	16: 96,068,572 (GRCm38)	I85N	probably damaging	Het
Ccdc24	T	A	4: 117,870,535 (GRCm38)	N145I	possibly damaging	Het
Cdhr5	G	T	7: 141,272,531 (GRCm38)	Q141K	probably damaging	Het
Cfap46	A	G	7: 139,619,971 (GRCm38)	V1998A	possibly damaging	Het
Csmd1	T	A	8: 16,002,626 (GRCm38)	Y2166F	probably damaging	Het
Cul4a	T	G	8: 13,136,219 (GRCm38)	S474A	probably benign	Het
Cutal	A	G	2: 34,888,137 (GRCm38)	T112A	probably benign	Het
Dcaf6	A	C	1: 165,399,785 (GRCm38)	S258A	possibly damaging	Het
Dek	C	T	13: 47,099,390 (GRCm38)	V180M	probably damaging	Het
Dspp	C	A	5: 104,178,175 (GRCm38)	D801E	unknown	Het
Egflam	T	C	15: 7,219,725 (GRCm38)	T871A	probably damaging	Het
Erbin	A	T	13: 103,834,766 (GRCm38)	S781T	possibly damaging	Het
Erich6	A	T	3: 58,625,054 (GRCm38)	H377Q	probably damaging	Het
Exo5	C	A	4: 120,921,756 (GRCm38)	G304V	probably damaging	Het
Eya2	C	A	2: 165,716,037 (GRCm38)	S184R	possibly damaging	Het
Fhod1	G	A	8: 105,337,890 (GRCm38)		probably benign	Het
G6pc3	A	G	11: 102,193,670 (GRCm38)	Y302C	possibly damaging	Het
Gart	T	C	16: 91,636,107 (GRCm38)	D318G	probably benign	Het
Gm10300	T	C	4: 132,074,935 (GRCm38)		probably benign	Het
Gm11937	A	G	11: 99,610,074 (GRCm38)	V39A	probably damaging	Het
Gm16432	T	A	1: 178,017,712 (GRCm38)	Y99*	probably null	Het
Gm21994	T	A	2: 150,255,278 (GRCm38)	Y77F	possibly damaging	Het
Gnas	T	C	2: 174,334,251 (GRCm38)	M60T	probably damaging	Het
Grk5	G	T	19: 60,890,626 (GRCm38)	R16L	probably damaging	Het
Hcn3	A	G	3: 89,152,674 (GRCm38)	L221P	probably damaging	Het
Hddc3	G	A	7: 80,343,196 (GRCm38)	R20Q	possibly damaging	Het
Hectd4	T	C	5: 121,277,725 (GRCm38)	Y530H	possibly damaging	Het
Igf2bp1	A	T	11: 95,973,122 (GRCm38)	H247Q	probably benign	Het
Igkv4-70	T	A	6: 69,267,928 (GRCm38)	D103V	probably damaging	Het
Itpr2	T	A	6: 146,325,170 (GRCm38)	M1359L	probably damaging	Het
Kalrn	A	G	16: 34,217,923 (GRCm38)	L1013S	probably damaging	Het
Kctd21	C	T	7: 97,348,084 (GRCm38)	R255W	probably damaging	Het
Krt18	T	A	15: 102,030,769 (GRCm38)	Y263N	probably benign	Het
Lamp5	C	G	2: 136,059,563 (GRCm38)	N102K	possibly damaging	Het
Larp1	G	A	11: 58,042,647 (GRCm38)		probably null	Het
Lmnb1	A	G	18: 56,728,469 (GRCm38)	N144S	probably damaging	Het
Lrp2	T	A	2: 69,448,211 (GRCm38)	T3933S	probably benign	Het
Lrrc34	T	C	3: 30,624,859 (GRCm38)	N363S	probably benign	Het
Lrriq1	T	C	10: 103,181,889 (GRCm38)		probably null	Het
Mafa	C	A	15: 75,747,780 (GRCm38)	G48V	unknown	Het
Mboat4	T	C	8: 34,124,521 (GRCm38)	S371P	possibly damaging	Het
Mei4	A	T	9: 82,025,624 (GRCm38)	M237L	probably benign	Het
Mfsd2a	T	C	4: 122,951,261 (GRCm38)	D219G	probably benign	Het
Msl2	A	G	9: 101,101,002 (GRCm38)	N192D	probably damaging	Het
Mycbp2	C	A	14: 103,191,567 (GRCm38)	R2358M	probably null	Het
Myh10	A	G	11: 68,745,339 (GRCm38)	T185A	probably damaging	Het
Nipsnap2	T	C	5: 129,745,288 (GRCm38)		probably null	Het
Notch1	T	C	2: 26,460,286 (GRCm38)	T2281A	probably benign	Het
Olfr1165-ps	C	A	2: 88,101,603 (GRCm38)	C128F	probably benign	Het
Olfr1436	G	A	19: 12,298,572 (GRCm38)	Q187*	probably null	Het
Olfr376	G	T	11: 73,375,576 (GRCm38)	V276F	probably benign	Het
Olfr533	G	T	7: 140,466,921 (GRCm38)	C240F	probably damaging	Het
Olfr533	T	C	7: 140,466,887 (GRCm38)	S229P	probably damaging	Het
Olfr735	A	T	14: 50,345,448 (GRCm38)	N300K	probably damaging	Het
Olfr895	T	A	9: 38,268,570 (GRCm38)	I19N	probably damaging	Het
Olfr948	A	G	9: 39,318,793 (GRCm38)	S274P	probably damaging	Het
Oxct1	T	A	15: 4,092,417 (GRCm38)	S283T	probably benign	Het
Pcnx2	G	T	8: 125,752,317 (GRCm38)		probably null	Het
Piwil4	A	G	9: 14,715,823 (GRCm38)	F424L	probably benign	Het
Pkhd1l1	T	C	15: 44,557,940 (GRCm38)	S3035P	probably damaging	Het
Psmc2	T	A	5: 21,800,576 (GRCm38)	D218E	probably damaging	Het
Ptpn7	C	T	1: 135,139,236 (GRCm38)	P277L	probably benign	Het
Rgs12	A	G	5: 35,023,092 (GRCm38)	K27E	probably damaging	Het
Rp1l1	G	T	14: 64,029,724 (GRCm38)	A920S	possibly damaging	Het
Rsbn1l	A	T	5: 20,908,224 (GRCm38)	H433Q	probably benign	Het
Safb	C	A	17: 56,606,023 (GRCm38)	P913Q	possibly damaging	Het
Sema7a	G	A	9: 57,960,571 (GRCm38)	V477M	probably damaging	Het
Serpina16	T	A	12: 103,668,932 (GRCm38)	T408S	possibly damaging	Het
Six5	G	A	7: 19,094,991 (GRCm38)	V119M	possibly damaging	Het
Slc6a16	C	T	7: 45,259,028 (GRCm38)	P11S	possibly damaging	Het
Sntg1	T	A	1: 8,445,050 (GRCm38)	I420F	probably benign	Het
Sptb	T	C	12: 76,613,180 (GRCm38)	D982G	possibly damaging	Het
Stag3	T	A	5: 138,301,499 (GRCm38)	F891I	probably damaging	Het
Sugp1	A	G	8: 70,059,303 (GRCm38)	E183G	probably benign	Het
Taf1d	T	C	9: 15,307,823 (GRCm38)		probably null	Het
Tapbp	T	C	17: 33,919,957 (GRCm38)	S33P	possibly damaging	Het
Ubap2	CT	CTTGCCCCGGT	4: 41,227,224 (GRCm38)		probably benign	Het
Ubap2	GCCCGCTTGCCCCGCT	GCCCGCTTGCCCCGCTTGCCCCGCT	4: 41,227,210 (GRCm38)		probably benign	Het
Ubash3a	A	G	17: 31,231,415 (GRCm38)	T355A	probably benign	Het
Usp16	T	G	16: 87,470,397 (GRCm38)	V225G	probably damaging	Het
Utrn	A	G	10: 12,621,303 (GRCm38)	V2454A	probably benign	Het
Vmn1r191	G	T	13: 22,179,550 (GRCm38)	F11L	probably benign	Het
Vmn2r117	A	T	17: 23,478,308 (GRCm38)	C137S	probably damaging	Het
Zfp143	T	C	7: 110,091,814 (GRCm38)	M524T	probably damaging	Het
Zfp599	A	G	9: 22,249,844 (GRCm38)	C342R	probably damaging	Het
Zfp777	T	G	6: 48,024,856 (GRCm38)	K811Q	probably damaging	Het
Zfp870	T	A	17: 32,883,596 (GRCm38)	H254L	probably benign	Het

Other mutations in Smg1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00272:Smg1	APN	7	118,198,271 (GRCm38)	utr 3 prime	probably benign
IGL00481:Smg1	APN	7	118,210,794 (GRCm38)	missense	possibly damaging	0.67
IGL00503:Smg1	APN	7	118,185,483 (GRCm38)	utr 3 prime	probably benign
IGL00927:Smg1	APN	7	118,140,632 (GRCm38)	missense	probably damaging	1.00
IGL01333:Smg1	APN	7	118,163,378 (GRCm38)	splice site	probably benign
IGL01344:Smg1	APN	7	118,190,836 (GRCm38)	utr 3 prime	probably benign
IGL01397:Smg1	APN	7	118,163,221 (GRCm38)	utr 3 prime	probably benign
IGL01403:Smg1	APN	7	118,158,132 (GRCm38)	utr 3 prime	probably benign
IGL01573:Smg1	APN	7	118,167,962 (GRCm38)	utr 3 prime	probably benign
IGL01872:Smg1	APN	7	118,148,944 (GRCm38)	utr 3 prime	probably benign
IGL02010:Smg1	APN	7	118,186,146 (GRCm38)	utr 3 prime	probably benign
IGL02158:Smg1	APN	7	118,212,946 (GRCm38)	missense	possibly damaging	0.77
IGL02268:Smg1	APN	7	118,182,541 (GRCm38)	missense	probably benign	0.19
IGL02314:Smg1	APN	7	118,154,709 (GRCm38)	utr 3 prime	probably benign
IGL02552:Smg1	APN	7	118,195,894 (GRCm38)	utr 3 prime	probably benign
IGL02577:Smg1	APN	7	118,203,122 (GRCm38)	missense	probably damaging	0.99
IGL02859:Smg1	APN	7	118,148,933 (GRCm38)	utr 3 prime	probably benign
IGL02890:Smg1	APN	7	118,185,501 (GRCm38)	utr 3 prime	probably benign
IGL02892:Smg1	APN	7	118,167,955 (GRCm38)	utr 3 prime	probably benign
IGL03119:Smg1	APN	7	118,195,113 (GRCm38)	utr 3 prime	probably benign
IGL03123:Smg1	APN	7	118,157,181 (GRCm38)	utr 3 prime	probably benign
IGL03128:Smg1	APN	7	118,203,059 (GRCm38)	missense	probably benign	0.03
IGL03184:Smg1	APN	7	118,180,380 (GRCm38)	missense	possibly damaging	0.86
PIT4508001:Smg1	UTSW	7	118,185,541 (GRCm38)	missense	unknown
R0010:Smg1	UTSW	7	118,171,859 (GRCm38)	utr 3 prime	probably benign
R0010:Smg1	UTSW	7	118,171,859 (GRCm38)	utr 3 prime	probably benign
R0025:Smg1	UTSW	7	118,212,443 (GRCm38)	missense	possibly damaging	0.92
R0025:Smg1	UTSW	7	118,212,443 (GRCm38)	missense	possibly damaging	0.92
R0098:Smg1	UTSW	7	118,145,467 (GRCm38)	missense	probably benign	0.02
R0139:Smg1	UTSW	7	118,152,675 (GRCm38)	critical splice donor site	probably null
R0371:Smg1	UTSW	7	118,168,300 (GRCm38)	utr 3 prime	probably benign
R0415:Smg1	UTSW	7	118,182,468 (GRCm38)	missense	probably benign	0.34
R0416:Smg1	UTSW	7	118,184,461 (GRCm38)	splice site	probably benign
R0423:Smg1	UTSW	7	118,176,880 (GRCm38)	missense	possibly damaging	0.53
R0600:Smg1	UTSW	7	118,160,383 (GRCm38)	utr 3 prime	probably benign
R0626:Smg1	UTSW	7	118,182,383 (GRCm38)	missense	possibly damaging	0.82
R0627:Smg1	UTSW	7	118,167,861 (GRCm38)	utr 3 prime	probably benign
R0727:Smg1	UTSW	7	118,166,422 (GRCm38)	utr 3 prime	probably benign
R0729:Smg1	UTSW	7	118,146,289 (GRCm38)	utr 3 prime	probably benign
R0841:Smg1	UTSW	7	118,143,301 (GRCm38)	missense	possibly damaging	0.96
R1114:Smg1	UTSW	7	118,159,790 (GRCm38)	utr 3 prime	probably benign
R1256:Smg1	UTSW	7	118,203,087 (GRCm38)	missense	probably damaging	1.00
R1298:Smg1	UTSW	7	118,168,211 (GRCm38)	utr 3 prime	probably benign
R1370:Smg1	UTSW	7	118,159,752 (GRCm38)	utr 3 prime	probably benign
R1591:Smg1	UTSW	7	118,156,919 (GRCm38)	utr 3 prime	probably benign
R1736:Smg1	UTSW	7	118,165,967 (GRCm38)	splice site	probably null
R1755:Smg1	UTSW	7	118,203,064 (GRCm38)	nonsense	probably null
R1765:Smg1	UTSW	7	118,139,715 (GRCm38)	missense	probably benign	0.03
R1789:Smg1	UTSW	7	118,145,798 (GRCm38)	missense	possibly damaging	0.73
R1845:Smg1	UTSW	7	118,154,622 (GRCm38)	utr 3 prime	probably benign
R1908:Smg1	UTSW	7	118,154,199 (GRCm38)	utr 3 prime	probably benign
R1909:Smg1	UTSW	7	118,154,199 (GRCm38)	utr 3 prime	probably benign
R1942:Smg1	UTSW	7	118,158,103 (GRCm38)	utr 3 prime	probably benign
R2064:Smg1	UTSW	7	118,156,867 (GRCm38)	utr 3 prime	probably benign
R2072:Smg1	UTSW	7	118,163,166 (GRCm38)	utr 3 prime	probably benign
R2154:Smg1	UTSW	7	118,158,076 (GRCm38)	utr 3 prime	probably benign
R2895:Smg1	UTSW	7	118,189,143 (GRCm38)	utr 3 prime	probably benign
R2915:Smg1	UTSW	7	118,210,879 (GRCm38)	splice site	probably benign
R3416:Smg1	UTSW	7	118,148,853 (GRCm38)	utr 3 prime	probably benign
R3417:Smg1	UTSW	7	118,148,853 (GRCm38)	utr 3 prime	probably benign
R3873:Smg1	UTSW	7	118,154,662 (GRCm38)	utr 3 prime	probably benign
R4082:Smg1	UTSW	7	118,160,246 (GRCm38)	utr 3 prime	probably benign
R4230:Smg1	UTSW	7	118,148,733 (GRCm38)	critical splice donor site	probably null
R4304:Smg1	UTSW	7	118,139,518 (GRCm38)	missense	probably benign	0.03
R4549:Smg1	UTSW	7	118,159,683 (GRCm38)	utr 3 prime	probably benign
R4571:Smg1	UTSW	7	118,139,465 (GRCm38)	missense	possibly damaging	0.72
R4638:Smg1	UTSW	7	118,195,926 (GRCm38)	utr 3 prime	probably benign
R4642:Smg1	UTSW	7	118,154,264 (GRCm38)	utr 3 prime	probably benign
R4656:Smg1	UTSW	7	118,212,951 (GRCm38)	missense	probably benign	0.00
R4754:Smg1	UTSW	7	118,156,731 (GRCm38)	utr 3 prime	probably benign
R4798:Smg1	UTSW	7	118,180,474 (GRCm38)	missense	probably benign	0.32
R4906:Smg1	UTSW	7	118,152,408 (GRCm38)	utr 3 prime	probably benign
R4978:Smg1	UTSW	7	118,154,247 (GRCm38)	utr 3 prime	probably benign
R4989:Smg1	UTSW	7	118,208,051 (GRCm38)	missense	probably benign
R4989:Smg1	UTSW	7	118,158,100 (GRCm38)	utr 3 prime	probably benign
R5026:Smg1	UTSW	7	118,193,545 (GRCm38)	utr 3 prime	probably benign
R5124:Smg1	UTSW	7	118,213,012 (GRCm38)	missense	probably benign	0.00
R5318:Smg1	UTSW	7	118,160,204 (GRCm38)	utr 3 prime	probably benign
R5356:Smg1	UTSW	7	118,195,133 (GRCm38)	utr 3 prime	probably benign
R5404:Smg1	UTSW	7	118,206,908 (GRCm38)	missense	probably damaging	1.00
R5423:Smg1	UTSW	7	118,146,071 (GRCm38)	missense	possibly damaging	0.70
R5441:Smg1	UTSW	7	118,195,081 (GRCm38)	utr 3 prime	probably benign
R5490:Smg1	UTSW	7	118,139,436 (GRCm38)	missense	possibly damaging	0.86
R5541:Smg1	UTSW	7	118,157,163 (GRCm38)	utr 3 prime	probably benign
R5564:Smg1	UTSW	7	118,189,819 (GRCm38)	utr 3 prime	probably benign
R5580:Smg1	UTSW	7	118,148,902 (GRCm38)	utr 3 prime	probably benign
R5600:Smg1	UTSW	7	118,167,884 (GRCm38)	utr 3 prime	probably benign
R5628:Smg1	UTSW	7	118,154,701 (GRCm38)	utr 3 prime	probably benign
R5646:Smg1	UTSW	7	118,212,559 (GRCm38)	missense	probably benign	0.42
R5656:Smg1	UTSW	7	118,154,664 (GRCm38)	utr 3 prime	probably benign
R5660:Smg1	UTSW	7	118,143,347 (GRCm38)	missense	probably benign	0.33
R5706:Smg1	UTSW	7	118,145,590 (GRCm38)	missense	possibly damaging	0.86
R5786:Smg1	UTSW	7	118,212,897 (GRCm38)	missense	probably benign	0.12
R5890:Smg1	UTSW	7	118,190,586 (GRCm38)	utr 3 prime	probably benign
R5912:Smg1	UTSW	7	118,154,586 (GRCm38)	utr 3 prime	probably benign
R5977:Smg1	UTSW	7	118,141,357 (GRCm38)	utr 3 prime	probably benign
R5993:Smg1	UTSW	7	118,140,509 (GRCm38)	missense	probably benign	0.33
R6161:Smg1	UTSW	7	118,163,330 (GRCm38)	utr 3 prime	probably benign
R6187:Smg1	UTSW	7	118,189,163 (GRCm38)	utr 3 prime	probably benign
R6264:Smg1	UTSW	7	118,166,087 (GRCm38)	utr 3 prime	probably benign
R6331:Smg1	UTSW	7	118,154,277 (GRCm38)	utr 3 prime	probably benign
R6561:Smg1	UTSW	7	118,166,077 (GRCm38)	utr 3 prime	probably benign
R6571:Smg1	UTSW	7	118,184,514 (GRCm38)	utr 3 prime	probably benign
R6752:Smg1	UTSW	7	118,163,316 (GRCm38)	utr 3 prime	probably benign
R6777:Smg1	UTSW	7	118,189,117 (GRCm38)	utr 3 prime	probably benign
R6788:Smg1	UTSW	7	118,184,571 (GRCm38)	utr 3 prime	probably benign
R6883:Smg1	UTSW	7	118,168,180 (GRCm38)	utr 3 prime	probably benign
R6991:Smg1	UTSW	7	118,167,868 (GRCm38)	utr 3 prime	probably benign
R7056:Smg1	UTSW	7	118,146,400 (GRCm38)	splice site	probably benign
R7058:Smg1	UTSW	7	118,198,279 (GRCm38)	utr 3 prime	probably benign
R7100:Smg1	UTSW	7	118,184,520 (GRCm38)	missense	unknown
R7133:Smg1	UTSW	7	118,152,908 (GRCm38)	missense	unknown
R7221:Smg1	UTSW	7	118,182,797 (GRCm38)	missense	possibly damaging	0.86
R7229:Smg1	UTSW	7	118,176,955 (GRCm38)	missense	probably benign	0.03
R7293:Smg1	UTSW	7	118,166,099 (GRCm38)	missense	unknown
R7361:Smg1	UTSW	7	118,184,977 (GRCm38)	missense	unknown
R7438:Smg1	UTSW	7	118,195,893 (GRCm38)	missense	unknown
R7686:Smg1	UTSW	7	118,167,858 (GRCm38)	missense	unknown
R7798:Smg1	UTSW	7	118,171,939 (GRCm38)	missense	possibly damaging	0.73
R7908:Smg1	UTSW	7	118,186,134 (GRCm38)	missense	unknown
R7923:Smg1	UTSW	7	118,143,322 (GRCm38)	missense	possibly damaging	0.96
R7978:Smg1	UTSW	7	118,193,655 (GRCm38)	missense	unknown
R7997:Smg1	UTSW	7	118,173,142 (GRCm38)	missense	unknown
R7997:Smg1	UTSW	7	118,173,141 (GRCm38)	missense	unknown
R8025:Smg1	UTSW	7	118,206,989 (GRCm38)	nonsense	probably null
R8056:Smg1	UTSW	7	118,160,366 (GRCm38)	missense	unknown
R8061:Smg1	UTSW	7	118,152,387 (GRCm38)	missense	unknown
R8095:Smg1	UTSW	7	118,173,062 (GRCm38)	missense	unknown
R8198:Smg1	UTSW	7	118,145,606 (GRCm38)	missense	probably benign	0.03
R8399:Smg1	UTSW	7	118,190,571 (GRCm38)	missense	unknown
R8445:Smg1	UTSW	7	118,136,977 (GRCm38)	missense	possibly damaging	0.72
R8519:Smg1	UTSW	7	118,171,759 (GRCm38)	utr 3 prime	probably benign
R8817:Smg1	UTSW	7	118,159,664 (GRCm38)	missense	unknown
R8832:Smg1	UTSW	7	118,139,783 (GRCm38)	missense	probably benign	0.33
R8855:Smg1	UTSW	7	118,206,899 (GRCm38)	missense	unknown
R8866:Smg1	UTSW	7	118,206,899 (GRCm38)	missense	unknown
R8946:Smg1	UTSW	7	118,152,677 (GRCm38)	missense	probably null
R8954:Smg1	UTSW	7	118,206,992 (GRCm38)	missense	probably damaging	1.00
R8967:Smg1	UTSW	7	118,166,516 (GRCm38)	missense	unknown
R9072:Smg1	UTSW	7	118,183,809 (GRCm38)	missense	unknown
R9090:Smg1	UTSW	7	118,212,563 (GRCm38)	missense	unknown
R9156:Smg1	UTSW	7	118,154,661 (GRCm38)	missense	unknown
R9198:Smg1	UTSW	7	118,195,956 (GRCm38)	missense	unknown
R9240:Smg1	UTSW	7	118,139,808 (GRCm38)	missense	probably benign	0.18
R9271:Smg1	UTSW	7	118,212,563 (GRCm38)	missense	unknown
R9289:Smg1	UTSW	7	118,145,416 (GRCm38)	missense	possibly damaging	0.53
R9378:Smg1	UTSW	7	118,178,775 (GRCm38)	nonsense	probably null
R9396:Smg1	UTSW	7	118,208,080 (GRCm38)	missense	unknown
R9469:Smg1	UTSW	7	118,140,551 (GRCm38)	missense	possibly damaging	0.72
R9539:Smg1	UTSW	7	118,145,753 (GRCm38)	missense	probably benign	0.03
R9549:Smg1	UTSW	7	118,196,031 (GRCm38)	missense	unknown
R9563:Smg1	UTSW	7	118,212,985 (GRCm38)	missense	unknown
R9564:Smg1	UTSW	7	118,212,985 (GRCm38)	missense	unknown
R9597:Smg1	UTSW	7	118,213,047 (GRCm38)	missense	unknown
R9643:Smg1	UTSW	7	118,156,710 (GRCm38)	missense	unknown
R9703:Smg1	UTSW	7	118,140,521 (GRCm38)	missense	possibly damaging	0.73
R9730:Smg1	UTSW	7	118,183,781 (GRCm38)	missense	unknown
Z1088:Smg1	UTSW	7	118,178,399 (GRCm38)	missense	possibly damaging	0.96
Z1088:Smg1	UTSW	7	118,168,661 (GRCm38)	nonsense	probably null
Z1088:Smg1	UTSW	7	118,154,635 (GRCm38)	utr 3 prime	probably benign
Z1176:Smg1	UTSW	7	118,206,907 (GRCm38)	missense	unknown
Z1176:Smg1	UTSW	7	118,206,887 (GRCm38)	missense	unknown
Z1177:Smg1	UTSW	7	118,213,033 (GRCm38)	missense	unknown
Z1177:Smg1	UTSW	7	118,168,608 (GRCm38)	missense	probably null

Predicted Primers

PCR Primer
(F):5'- ACTTTCTCGGGAACTCTAAGGC -3'
(R):5'- TGTGCAGCAGTCTTAAGGAG -3'

Sequencing Primer
(F):5'- CGGGAACTCTAAGGCTTTTACC -3'
(R):5'- CAGTCTTAAGGAGTGCTTTGTG -3'

Posted On

2018-07-24