Mutagenetix > Incidental Mutation

Incidental Mutation 'R6698:Gpr158'

528636

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

044816-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6698 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21367542-21830547 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 21827110 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Alanine at position 1007 (D1007A)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably damaging
Transcript: ENSMUST00000055946
AA Change: D1007A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: D1007A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.0%

Validation Efficiency

95% (40/42)

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Brca2	T	C	5: 150,532,394 (GRCm38)	V200A	probably damaging	Het
Camk2g	T	A	14: 20,742,708 (GRCm38)	K393*	probably null	Het
Catsperb	T	A	12: 101,509,207 (GRCm38)	F337I	probably damaging	Het
Cdk17	T	C	10: 93,228,678 (GRCm38)	Y270H	probably damaging	Het
Col5a3	C	T	9: 20,779,033 (GRCm38)	G1162R	probably damaging	Het
Col6a5	T	A	9: 105,934,175 (GRCm38)	N715I	unknown	Het
Fam198b	A	G	3: 79,936,595 (GRCm38)	I10V	probably damaging	Het
Fancg	A	G	4: 43,007,034 (GRCm38)	S248P	probably benign	Het
Flvcr1	T	C	1: 191,025,732 (GRCm38)	Y79C	probably damaging	Het
Gabrp	A	G	11: 33,557,017 (GRCm38)	S198P	probably damaging	Het
Glp1r	A	G	17: 30,936,401 (GRCm38)	Y454C	probably damaging	Het
Gsdmc3	A	G	15: 63,860,271 (GRCm38)	F302S	possibly damaging	Het
Gsdmc4	T	A	15: 63,893,764 (GRCm38)	D312V	probably benign	Het
Itga5	T	C	15: 103,351,381 (GRCm38)	Y663C	probably benign	Het
Kif1b	A	T	4: 149,274,956 (GRCm38)	M108K	probably damaging	Het
Klf11	T	G	12: 24,653,619 (GRCm38)	S18A	probably damaging	Het
Lmtk2	T	C	5: 144,174,919 (GRCm38)	V819A	probably benign	Het
Lrba	A	G	3: 86,304,425 (GRCm38)	M451V	probably damaging	Het
Lrp1b	T	C	2: 41,302,946 (GRCm38)	D488G	probably damaging	Het
Mark4	T	C	7: 19,429,437 (GRCm38)	N589S	probably benign	Het
Mis12	T	C	11: 71,025,186 (GRCm38)	F15S	probably damaging	Het
Nif3l1	A	G	1: 58,450,489 (GRCm38)	D179G	probably benign	Het
Nlrx1	A	G	9: 44,265,807 (GRCm38)	W3R	probably damaging	Het
Nup210l	G	A	3: 90,182,508 (GRCm38)	S1194N	possibly damaging	Het
Olfr1165-ps	A	G	2: 88,101,217 (GRCm38)	F257L	unknown	Het
Olfr451-ps1	A	T	6: 42,801,202 (GRCm38)	T154S	probably benign	Het
Park2	A	G	17: 11,067,296 (GRCm38)		probably null	Het
Pnkd	T	A	1: 74,350,677 (GRCm38)	L320Q	probably damaging	Het
Rcc2	G	A	4: 140,702,275 (GRCm38)	C40Y	probably benign	Homo
Rilpl2	C	T	5: 124,469,780 (GRCm38)	E126K	probably damaging	Het
Rpn2	T	C	2: 157,297,410 (GRCm38)	I208T	possibly damaging	Het
Skint4	G	T	4: 112,119,899 (GRCm38)	C170F	probably damaging	Het
Synj1	C	G	16: 90,960,452 (GRCm38)	V877L	probably damaging	Het
Tcp11	A	T	17: 28,071,830 (GRCm38)	I106N	possibly damaging	Het
Tg	A	G	15: 66,839,362 (GRCm38)	Y991C	probably damaging	Het
Trib3	A	G	2: 152,338,419 (GRCm38)	S285P	probably damaging	Het
Wdr49	A	T	3: 75,429,366 (GRCm38)	W345R	probably benign	Het
Wnt5a	A	G	14: 28,518,463 (GRCm38)	Y190C	possibly damaging	Het
Xpo1	A	G	11: 23,294,040 (GRCm38)	E955G	probably benign	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,368,683 (GRCm38)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,746,795 (GRCm38)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,746,773 (GRCm38)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,826,818 (GRCm38)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,649,021 (GRCm38)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,369,031 (GRCm38)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,827,098 (GRCm38)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,783,290 (GRCm38)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,368,700 (GRCm38)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,826,596 (GRCm38)	missense	probably benign
IGL02681:Gpr158	APN	2	21,815,630 (GRCm38)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,826,827 (GRCm38)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,827,079 (GRCm38)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,783,161 (GRCm38)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,825,274 (GRCm38)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,826,246 (GRCm38)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,826,871 (GRCm38)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,810,668 (GRCm38)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,826,717 (GRCm38)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,825,208 (GRCm38)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,825,274 (GRCm38)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,815,669 (GRCm38)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,826,314 (GRCm38)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,815,694 (GRCm38)	splice site	probably null
R1609:Gpr158	UTSW	2	21,783,293 (GRCm38)	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21,827,548 (GRCm38)	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21,827,318 (GRCm38)	missense	probably benign
R1854:Gpr158	UTSW	2	21,369,124 (GRCm38)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,815,615 (GRCm38)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,827,514 (GRCm38)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,826,863 (GRCm38)	missense	probably benign
R2275:Gpr158	UTSW	2	21,826,863 (GRCm38)	missense	probably benign
R3004:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,576,960 (GRCm38)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,368,559 (GRCm38)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,368,551 (GRCm38)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,825,214 (GRCm38)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,827,592 (GRCm38)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4506:Gpr158	UTSW	2	21,826,999 (GRCm38)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,369,000 (GRCm38)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,827,053 (GRCm38)	missense	probably benign
R4798:Gpr158	UTSW	2	21,783,182 (GRCm38)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,825,248 (GRCm38)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,827,157 (GRCm38)	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21,827,505 (GRCm38)	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21,826,290 (GRCm38)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,827,235 (GRCm38)	missense	probably benign
R5637:Gpr158	UTSW	2	21,783,272 (GRCm38)	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21,746,709 (GRCm38)	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21,368,520 (GRCm38)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,369,121 (GRCm38)	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21,368,508 (GRCm38)	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21,399,416 (GRCm38)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,815,611 (GRCm38)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,810,554 (GRCm38)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,826,288 (GRCm38)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,648,991 (GRCm38)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,826,575 (GRCm38)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,368,302 (GRCm38)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,810,601 (GRCm38)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,576,939 (GRCm38)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,827,318 (GRCm38)	missense	probably benign
R7515:Gpr158	UTSW	2	21,368,281 (GRCm38)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,826,347 (GRCm38)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,826,863 (GRCm38)	missense	probably benign
R8311:Gpr158	UTSW	2	21,368,890 (GRCm38)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,576,882 (GRCm38)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,399,338 (GRCm38)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,553,326 (GRCm38)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,576,940 (GRCm38)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,576,949 (GRCm38)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,825,267 (GRCm38)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,826,440 (GRCm38)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,368,231 (GRCm38)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,827,226 (GRCm38)	missense	probably benign
R9379:Gpr158	UTSW	2	21,368,231 (GRCm38)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,783,161 (GRCm38)	missense	probably benign
R9497:Gpr158	UTSW	2	21,827,014 (GRCm38)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,825,243 (GRCm38)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,826,504 (GRCm38)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,826,369 (GRCm38)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,810,690 (GRCm38)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,827,272 (GRCm38)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- AAGACCGAGCTAAGTCCCAG -3'
(R):5'- AGGGACTGACTGTGGATTTCCC -3'

Sequencing Primer
(F):5'- GACCATCAGGAAGTACTCAAATTCAG -3'
(R):5'- TGGATTTCCCAGGGGCACAC -3'

Posted On

2018-07-24