Incidental Mutation 'R6698:Trib3'
ID 528639
Institutional Source Beutler Lab
Gene Symbol Trib3
Ensembl Gene ENSMUSG00000032715
Gene Name tribbles pseudokinase 3
Synonyms Nipk, Ifld2, Trb3, SKIP3
MMRRC Submission
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock # R6698 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 152337422-152344032 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) A to G at 152338419 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Serine to Proline at position 285 (S285P)
Ref Sequence ENSEMBL: ENSMUSP00000041747 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000040312]
AlphaFold Q8K4K2
Predicted Effect probably damaging
Transcript: ENSMUST00000040312
AA Change: S285P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000041747
Gene: ENSMUSG00000032715
AA Change: S285P

low complexity region 39 65 N/A INTRINSIC
Pfam:Pkinase 78 315 3.2e-27 PFAM
Pfam:Pkinase_Tyr 102 307 1.2e-10 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000123145
Predicted Effect noncoding transcript
Transcript: ENSMUST00000124995
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.0%
  • 20x: 94.0%
Validation Efficiency 95% (40/42)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
PHENOTYPE: Homozygous null mutants may display hypoactivity, decreased blood pressure and abnormal digit morphology or abnormal mast cell physiology. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Brca2 T C 5: 150,532,394 V200A probably damaging Het
Camk2g T A 14: 20,742,708 K393* probably null Het
Catsperb T A 12: 101,509,207 F337I probably damaging Het
Cdk17 T C 10: 93,228,678 Y270H probably damaging Het
Col5a3 C T 9: 20,779,033 G1162R probably damaging Het
Col6a5 T A 9: 105,934,175 N715I unknown Het
Fam198b A G 3: 79,936,595 I10V probably damaging Het
Fancg A G 4: 43,007,034 S248P probably benign Het
Flvcr1 T C 1: 191,025,732 Y79C probably damaging Het
Gabrp A G 11: 33,557,017 S198P probably damaging Het
Glp1r A G 17: 30,936,401 Y454C probably damaging Het
Gpr158 A C 2: 21,827,110 D1007A probably damaging Het
Gsdmc3 A G 15: 63,860,271 F302S possibly damaging Het
Gsdmc4 T A 15: 63,893,764 D312V probably benign Het
Itga5 T C 15: 103,351,381 Y663C probably benign Het
Kif1b A T 4: 149,274,956 M108K probably damaging Het
Klf11 T G 12: 24,653,619 S18A probably damaging Het
Lmtk2 T C 5: 144,174,919 V819A probably benign Het
Lrba A G 3: 86,304,425 M451V probably damaging Het
Lrp1b T C 2: 41,302,946 D488G probably damaging Het
Mark4 T C 7: 19,429,437 N589S probably benign Het
Mis12 T C 11: 71,025,186 F15S probably damaging Het
Nif3l1 A G 1: 58,450,489 D179G probably benign Het
Nlrx1 A G 9: 44,265,807 W3R probably damaging Het
Nup210l G A 3: 90,182,508 S1194N possibly damaging Het
Olfr1165-ps A G 2: 88,101,217 F257L unknown Het
Olfr451-ps1 A T 6: 42,801,202 T154S probably benign Het
Park2 A G 17: 11,067,296 probably null Het
Pnkd T A 1: 74,350,677 L320Q probably damaging Het
Rcc2 G A 4: 140,702,275 C40Y probably benign Homo
Rilpl2 C T 5: 124,469,780 E126K probably damaging Het
Rpn2 T C 2: 157,297,410 I208T possibly damaging Het
Skint4 G T 4: 112,119,899 C170F probably damaging Het
Synj1 C G 16: 90,960,452 V877L probably damaging Het
Tcp11 A T 17: 28,071,830 I106N possibly damaging Het
Tg A G 15: 66,839,362 Y991C probably damaging Het
Wdr49 A T 3: 75,429,366 W345R probably benign Het
Wnt5a A G 14: 28,518,463 Y190C possibly damaging Het
Xpo1 A G 11: 23,294,040 E955G probably benign Het
Other mutations in Trib3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02303:Trib3 APN 2 152343150 missense probably benign 0.02
R2570:Trib3 UTSW 2 152343236 missense probably benign 0.01
R5637:Trib3 UTSW 2 152338490 missense probably damaging 1.00
R5759:Trib3 UTSW 2 152343295 missense probably benign 0.28
R6221:Trib3 UTSW 2 152338608 missense probably damaging 1.00
R7443:Trib3 UTSW 2 152339772 missense possibly damaging 0.90
R8048:Trib3 UTSW 2 152338633 missense probably damaging 1.00
R8827:Trib3 UTSW 2 152338268 missense possibly damaging 0.94
R9232:Trib3 UTSW 2 152343042 missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2018-07-24