Mutagenetix > Incidental Mutation

Incidental Mutation 'R6706:Vmn2r63'

528946

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r63

Ensembl Gene

ENSMUSG00000090751

Gene Name

vomeronasal 2, receptor 63

Synonyms

EG435975

MMRRC Submission

044824-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R6706 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42552675-42583213 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 42578001 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 179 (D179V)

Ref Sequence

ENSEMBL: ENSMUSP00000129089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000163803]

AlphaFold

E9Q0K5

Predicted Effect

probably damaging
Transcript: ENSMUST00000163803
AA Change: D179V

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000129089
Gene: ENSMUSG00000090751
AA Change: D179V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	3.5e-43	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	3.5e-51	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.2%

Validation Efficiency

97% (36/37)

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam34	T	A	8: 44,104,479 (GRCm39)	K389*	probably null	Het
Adam6a	T	G	12: 113,508,886 (GRCm39)	F420V	probably benign	Het
Alpl	T	C	4: 137,473,740 (GRCm39)	T321A	probably benign	Het
Asxl3	A	G	18: 22,586,666 (GRCm39)	D152G	probably damaging	Het
B4galt4	A	G	16: 38,578,173 (GRCm39)	T207A	probably benign	Het
Bsph2	A	T	7: 13,304,972 (GRCm39)	M1K	probably null	Het
Cacna2d1	T	A	5: 16,531,338 (GRCm39)	L535Q	probably damaging	Het
Cacna2d3	T	C	14: 28,846,642 (GRCm39)		probably null	Het
Chrdl2	T	C	7: 99,659,328 (GRCm39)		probably null	Het
Ctdnep1	A	G	11: 69,875,138 (GRCm39)	N54S	probably benign	Het
Dclre1a	A	T	19: 56,533,501 (GRCm39)	D364E	probably benign	Het
Dock1	A	G	7: 134,735,615 (GRCm39)	I1328V	possibly damaging	Het
Eno4	A	T	19: 58,959,112 (GRCm39)	E411D	probably benign	Het
Fbxl14	A	G	6: 119,457,716 (GRCm39)	Y299C	probably benign	Het
Fhdc1	T	C	3: 84,353,729 (GRCm39)	S499G	probably damaging	Het
Hectd4	C	T	5: 121,458,147 (GRCm39)	T771I	possibly damaging	Het
Kansl3	A	G	1: 36,383,995 (GRCm39)		probably null	Het
Letm2	G	A	8: 26,083,977 (GRCm39)	H85Y	probably benign	Het
Map3k6	A	T	4: 132,978,250 (GRCm39)	K1031*	probably null	Het
Mfsd4b5	T	A	10: 39,862,413 (GRCm39)	T37S	probably benign	Het
Mgam	T	A	6: 40,721,720 (GRCm39)	V346D	probably benign	Het
Myrip	C	T	9: 120,217,359 (GRCm39)	H98Y	possibly damaging	Het
Nos2	A	G	11: 78,835,549 (GRCm39)	N443D	possibly damaging	Het
Notch2	A	T	3: 98,045,746 (GRCm39)	D1637V	possibly damaging	Het
Or4c35	T	A	2: 89,808,929 (GRCm39)	V269E	probably damaging	Het
Or6z6	G	A	7: 6,491,049 (GRCm39)	R275C	probably damaging	Het
Pank1	C	T	19: 34,789,786 (GRCm39)	G530D	probably damaging	Het
Pde4dip	G	T	3: 97,648,709 (GRCm39)	R1036S	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Plin2	T	C	4: 86,578,357 (GRCm39)	T247A	probably benign	Het
Rp1	T	A	1: 4,212,887 (GRCm39)	I1067F	unknown	Het
Serpinb9d	T	A	13: 33,380,541 (GRCm39)	N142K	probably benign	Het
Slc12a5	A	G	2: 164,830,509 (GRCm39)	Y639C	probably damaging	Het
Tfap2c	A	G	2: 172,399,276 (GRCm39)	M508V	probably benign	Het
Tmprss11d	T	C	5: 86,478,962 (GRCm39)	N147S	probably benign	Het
Togaram1	T	A	12: 65,049,383 (GRCm39)	N1273K	probably benign	Het
Ttn	G	A	2: 76,709,687 (GRCm39)	R1581*	probably null	Het
Uggt2	T	A	14: 119,308,293 (GRCm39)	I363F	probably damaging	Het

Other mutations in Vmn2r63

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01399:Vmn2r63	APN	7	42,553,543 (GRCm39)	missense	probably damaging	0.99
IGL01765:Vmn2r63	APN	7	42,552,788 (GRCm39)	missense	probably benign
IGL02203:Vmn2r63	APN	7	42,553,432 (GRCm39)	missense	probably benign	0.01
IGL02825:Vmn2r63	APN	7	42,576,274 (GRCm39)	critical splice donor site	probably null
IGL03155:Vmn2r63	APN	7	42,552,878 (GRCm39)	missense	probably damaging	1.00
IGL03260:Vmn2r63	APN	7	42,578,616 (GRCm39)	missense	probably damaging	1.00
IGL03411:Vmn2r63	APN	7	42,577,368 (GRCm39)	missense	probably benign	0.42
PIT4280001:Vmn2r63	UTSW	7	42,553,409 (GRCm39)	missense	probably damaging	1.00
R0066:Vmn2r63	UTSW	7	42,576,514 (GRCm39)	splice site	probably benign
R0328:Vmn2r63	UTSW	7	42,552,699 (GRCm39)	missense	probably benign	0.09
R0344:Vmn2r63	UTSW	7	42,553,042 (GRCm39)	missense	probably damaging	0.98
R0554:Vmn2r63	UTSW	7	42,583,129 (GRCm39)	nonsense	probably null
R0555:Vmn2r63	UTSW	7	42,577,952 (GRCm39)	nonsense	probably null
R0685:Vmn2r63	UTSW	7	42,577,434 (GRCm39)	missense	probably benign	0.13
R0751:Vmn2r63	UTSW	7	42,577,459 (GRCm39)	missense	probably damaging	1.00
R1349:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1372:Vmn2r63	UTSW	7	42,578,642 (GRCm39)	missense	possibly damaging	0.95
R1416:Vmn2r63	UTSW	7	42,577,339 (GRCm39)	missense	probably benign	0.00
R1502:Vmn2r63	UTSW	7	42,578,015 (GRCm39)	missense	possibly damaging	0.62
R1563:Vmn2r63	UTSW	7	42,553,550 (GRCm39)	missense	probably benign	0.00
R1652:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R1693:Vmn2r63	UTSW	7	42,577,743 (GRCm39)	missense	probably benign
R1698:Vmn2r63	UTSW	7	42,583,038 (GRCm39)	missense	probably benign
R1753:Vmn2r63	UTSW	7	42,577,669 (GRCm39)	nonsense	probably null
R2136:Vmn2r63	UTSW	7	42,576,297 (GRCm39)	missense	probably damaging	0.99
R2175:Vmn2r63	UTSW	7	42,583,004 (GRCm39)	critical splice donor site	probably null
R2261:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2262:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R2263:Vmn2r63	UTSW	7	42,578,031 (GRCm39)	missense	probably benign	0.02
R3413:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3426:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3427:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R3802:Vmn2r63	UTSW	7	42,552,829 (GRCm39)	missense	probably damaging	0.99
R4319:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4321:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4323:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	probably benign	0.04
R4346:Vmn2r63	UTSW	7	42,577,537 (GRCm39)	missense	possibly damaging	0.67
R4568:Vmn2r63	UTSW	7	42,583,250 (GRCm39)	splice site	probably null
R4649:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4653:Vmn2r63	UTSW	7	42,553,114 (GRCm39)	missense	possibly damaging	0.79
R4679:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4734:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4741:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4748:Vmn2r63	UTSW	7	42,577,544 (GRCm39)	missense	probably benign	0.00
R4806:Vmn2r63	UTSW	7	42,576,314 (GRCm39)	missense	probably benign	0.34
R4933:Vmn2r63	UTSW	7	42,553,402 (GRCm39)	missense	probably damaging	1.00
R5198:Vmn2r63	UTSW	7	42,553,169 (GRCm39)	missense	probably benign	0.01
R5399:Vmn2r63	UTSW	7	42,577,701 (GRCm39)	missense	probably benign
R5400:Vmn2r63	UTSW	7	42,577,635 (GRCm39)	missense	probably benign	0.32
R6158:Vmn2r63	UTSW	7	42,583,104 (GRCm39)	missense	probably damaging	1.00
R6185:Vmn2r63	UTSW	7	42,578,435 (GRCm39)	missense	probably damaging	0.98
R6267:Vmn2r63	UTSW	7	42,578,059 (GRCm39)	splice site	probably null
R6362:Vmn2r63	UTSW	7	42,552,721 (GRCm39)	missense	probably benign
R6764:Vmn2r63	UTSW	7	42,552,695 (GRCm39)	missense	probably damaging	0.97
R7104:Vmn2r63	UTSW	7	42,577,959 (GRCm39)	missense	possibly damaging	0.67
R7503:Vmn2r63	UTSW	7	42,583,014 (GRCm39)	missense	probably benign	0.02
R7506:Vmn2r63	UTSW	7	42,576,391 (GRCm39)	missense	probably damaging	1.00
R7525:Vmn2r63	UTSW	7	42,576,406 (GRCm39)	missense	possibly damaging	0.52
R7658:Vmn2r63	UTSW	7	42,574,693 (GRCm39)	missense	probably damaging	1.00
R7663:Vmn2r63	UTSW	7	42,576,466 (GRCm39)	missense	probably benign	0.00
R7702:Vmn2r63	UTSW	7	42,577,553 (GRCm39)	missense	possibly damaging	0.46
R7918:Vmn2r63	UTSW	7	42,552,955 (GRCm39)	missense	probably damaging	1.00
R8826:Vmn2r63	UTSW	7	42,576,441 (GRCm39)	missense	probably benign	0.38
R8973:Vmn2r63	UTSW	7	42,577,919 (GRCm39)	missense	probably benign	0.19
R9024:Vmn2r63	UTSW	7	42,577,874 (GRCm39)	missense	probably benign	0.14
R9154:Vmn2r63	UTSW	7	42,576,413 (GRCm39)	missense	probably damaging	0.96
R9731:Vmn2r63	UTSW	7	42,553,361 (GRCm39)	missense	probably benign	0.32
Z1088:Vmn2r63	UTSW	7	42,577,983 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- GGTGCCAGGAATCATTTTCAC -3'
(R):5'- ACTTGATGACAGATCCACAATTCC -3'

Sequencing Primer
(F):5'- GTGCCAGGAATCATTTTCACAAAGG -3'
(R):5'- TGATGACAGATCCACAATTCCATTAC -3'

Posted On

2018-07-24