Mutagenetix > Incidental Mutation

Incidental Mutation 'R6717:Olfr142'

529419

Institutional Source

Beutler Lab

Gene Symbol

Olfr142

Ensembl Gene

ENSMUSG00000075063

Gene Name

olfactory receptor 142

Synonyms

GA_x6K02T2Q125-51607674-51606757, K20, MOR227-2

MMRRC Submission

044835-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R6717 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

90250130-90257592 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 90252524 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 155 (I155L)

Ref Sequence

ENSEMBL: ENSMUSP00000150216 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099752] [ENSMUST00000213968]

AlphaFold

Q60881

Predicted Effect

probably benign
Transcript: ENSMUST00000099752
AA Change: I155L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000097341
Gene: ENSMUSG00000075063
AA Change: I155L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	303	4.3e-53	PFAM
Pfam:7TM_GPCR_Srsx	33	300	3e-6	PFAM
Pfam:7tm_1	39	285	5.8e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213968
AA Change: I155L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 96.5%

Validation Efficiency

98% (47/48)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap9	C	T	5: 4,064,086 (GRCm38)	L3122F	probably damaging	Het
Atp6v1b1	A	T	6: 83,753,650 (GRCm38)		probably null	Het
Ccdc178	A	T	18: 22,020,889 (GRCm38)	V621E	probably damaging	Het
Cfap126	T	C	1: 171,114,102 (GRCm38)		probably null	Het
Cog2	T	C	8: 124,525,749 (GRCm38)	I64T	probably damaging	Het
Dhx9	A	C	1: 153,473,464 (GRCm38)		probably null	Het
Efcab7	A	G	4: 99,904,734 (GRCm38)	D407G	possibly damaging	Het
Eif2b5	G	T	16: 20,505,283 (GRCm38)	G459C	probably damaging	Het
Eml5	T	C	12: 98,827,506 (GRCm38)	E1168G	probably damaging	Het
Flnc	AGCTGTCAAGTATGCTG	AGCTG	6: 29,450,902 (GRCm38)		probably benign	Het
Fry	A	G	5: 150,496,312 (GRCm38)	T980A	probably benign	Het
Gabbr2	A	G	4: 46,787,574 (GRCm38)	V363A	possibly damaging	Het
Ggt6	T	A	11: 72,437,520 (GRCm38)	L244*	probably null	Het
Gm13119	G	T	4: 144,362,657 (GRCm38)	V182L	probably benign	Het
Gprc6a	T	A	10: 51,615,137 (GRCm38)	I768F	probably damaging	Het
Grk1	G	A	8: 13,416,237 (GRCm38)	M560I	probably benign	Het
Hapln4	G	A	8: 70,085,090 (GRCm38)	E145K	probably damaging	Het
Hoxd9	C	T	2: 74,698,389 (GRCm38)	P112S	probably benign	Het
Ly6g6f	T	A	17: 35,085,574 (GRCm38)	M1L	probably benign	Het
Mast1	T	C	8: 84,917,754 (GRCm38)	T849A	probably benign	Het
Mob4	A	T	1: 55,136,713 (GRCm38)	M39L	possibly damaging	Het
Mrgpre	A	T	7: 143,781,523 (GRCm38)	L81Q	probably damaging	Het
Mst1	T	C	9: 108,080,575 (GRCm38)		probably null	Het
Muc5b	A	T	7: 141,857,822 (GRCm38)	R1502*	probably null	Het
Olfr1020	T	A	2: 85,850,223 (GRCm38)	I257N	probably damaging	Het
Olfr311	T	C	11: 58,841,287 (GRCm38)	Y58H	probably damaging	Het
Pdc	A	G	1: 150,333,018 (GRCm38)	D84G	probably damaging	Het
Peak1	T	C	9: 56,207,239 (GRCm38)	N443D	probably benign	Het
Pkd1l3	T	A	8: 109,614,769 (GRCm38)	W85R	unknown	Het
Rfc1	A	G	5: 65,312,961 (GRCm38)	S68P	probably damaging	Het
Rfc1	G	A	5: 65,302,004 (GRCm38)	Q190*	probably null	Het
Rnf145	T	C	11: 44,561,490 (GRCm38)	V432A	probably benign	Het
Ror2	A	G	13: 53,118,982 (GRCm38)	S204P	probably damaging	Het
Scn1a	T	C	2: 66,332,287 (GRCm38)	E205G	probably damaging	Het
Slc2a8	A	C	2: 32,976,177 (GRCm38)	M277R	probably damaging	Het
Slc4a1	T	C	11: 102,354,423 (GRCm38)	Y566C	probably damaging	Het
Slc6a12	A	G	6: 121,354,303 (GRCm38)	N185S	probably benign	Het
Srcap	GCTCCTCCTCCTCCTCCT	GCTCCTCCTCCTCCT	7: 127,558,310 (GRCm38)		probably benign	Het
Stk33	T	A	7: 109,327,616 (GRCm38)	T279S	possibly damaging	Het
Taok3	A	G	5: 117,240,950 (GRCm38)		probably benign	Het
Tlr11	A	G	14: 50,362,104 (GRCm38)	T516A	probably benign	Het
Tmem132c	T	A	5: 127,564,029 (GRCm38)	L1088Q	possibly damaging	Het
Tmem132d	T	A	5: 127,784,421 (GRCm38)	M879L	probably benign	Het
Tnk2	A	G	16: 32,670,869 (GRCm38)	E322G	probably damaging	Het
Ttc28	T	C	5: 111,285,436 (GRCm38)	V2081A	probably benign	Het
Ttn	C	T	2: 76,794,409 (GRCm38)		probably null	Het
Zfp105	T	C	9: 122,930,308 (GRCm38)	V348A	possibly damaging	Het
Zswim2	T	C	2: 83,915,409 (GRCm38)	R562G	probably benign	Het

Other mutations in Olfr142

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Olfr142	APN	2	90,252,609 (GRCm38)	missense	probably damaging	1.00
IGL01623:Olfr142	APN	2	90,252,609 (GRCm38)	missense	probably damaging	1.00
IGL01810:Olfr142	APN	2	90,252,132 (GRCm38)	nonsense	probably null
IGL01918:Olfr142	APN	2	90,252,331 (GRCm38)	missense	probably damaging	1.00
IGL02619:Olfr142	APN	2	90,252,505 (GRCm38)	missense	probably damaging	0.97
IGL02732:Olfr142	APN	2	90,252,308 (GRCm38)	missense	probably damaging	1.00
IGL02738:Olfr142	APN	2	90,252,355 (GRCm38)	missense	possibly damaging	0.82
IGL02795:Olfr142	APN	2	90,252,562 (GRCm38)	missense	probably damaging	1.00
IGL02830:Olfr142	APN	2	90,252,781 (GRCm38)	missense	probably damaging	1.00
R0601:Olfr142	UTSW	2	90,252,934 (GRCm38)	missense	probably benign	0.05
R2004:Olfr142	UTSW	2	90,252,692 (GRCm38)	missense	probably benign	0.04
R2136:Olfr142	UTSW	2	90,252,253 (GRCm38)	missense	probably damaging	0.98
R2377:Olfr142	UTSW	2	90,252,911 (GRCm38)	missense	probably damaging	1.00
R3615:Olfr142	UTSW	2	90,252,409 (GRCm38)	missense	possibly damaging	0.94
R3616:Olfr142	UTSW	2	90,252,409 (GRCm38)	missense	possibly damaging	0.94
R3777:Olfr142	UTSW	2	90,252,625 (GRCm38)	missense	probably damaging	1.00
R4763:Olfr142	UTSW	2	90,252,463 (GRCm38)	missense	probably damaging	1.00
R4765:Olfr142	UTSW	2	90,252,463 (GRCm38)	missense	probably damaging	1.00
R5421:Olfr142	UTSW	2	90,252,745 (GRCm38)	missense	probably benign	0.01
R5426:Olfr142	UTSW	2	90,252,611 (GRCm38)	nonsense	probably null
R6063:Olfr142	UTSW	2	90,252,427 (GRCm38)	missense	probably benign	0.40
R6931:Olfr142	UTSW	2	90,252,777 (GRCm38)	nonsense	probably null
R6936:Olfr142	UTSW	2	90,252,334 (GRCm38)	missense	probably benign	0.17
R7013:Olfr142	UTSW	2	90,252,097 (GRCm38)	missense	possibly damaging	0.87
R7091:Olfr142	UTSW	2	90,252,463 (GRCm38)	missense	probably damaging	1.00
R7247:Olfr142	UTSW	2	90,252,821 (GRCm38)	missense	probably damaging	1.00
R8169:Olfr142	UTSW	2	90,252,098 (GRCm38)	nonsense	probably null
R8345:Olfr142	UTSW	2	90,252,217 (GRCm38)	missense	possibly damaging	0.50
R9222:Olfr142	UTSW	2	90,252,476 (GRCm38)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TCCTCAAGCTGATTAGGATGACAG -3'
(R):5'- TCTGAAGGGCTGTCTGACTCAG -3'

Sequencing Primer
(F):5'- TGACAGCATAGGAGGACACC -3'
(R):5'- AAGGGCTGTCTGACTCAGATATTC -3'

Posted On

2018-08-01