Mutagenetix > Incidental Mutation

Incidental Mutation 'R6727:Ugt1a10'

529890

Institutional Source

Beutler Lab

Gene Symbol

Ugt1a10

Ensembl Gene

ENSMUSG00000090165

Gene Name

UDP glycosyltransferase 1 family, polypeptide A10

Synonyms

A13

MMRRC Submission

044845-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.147) question?

Stock #

R6727 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

88055388-88219004 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 88056257 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134443 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000113142] [ENSMUST00000138182] [ENSMUST00000173325]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000113142
AA Change: L259*

SMART Domains

Protein: ENSMUSP00000108767
Gene: ENSMUSG00000090165
AA Change: L259*

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
Pfam:UDPGT	26	521	7.3e-231	PFAM
Pfam:Glyco_tran_28_C	360	449	1.3e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000138182

SMART Domains

Protein: ENSMUSP00000119985
Gene: ENSMUSG00000090165

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
Pfam:UDPGT	26	62	7e-11	PFAM
Pfam:UDPGT	58	207	1.9e-90	PFAM
Pfam:Glyco_tran_28_C	137	207	4.8e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173165

Predicted Effect

probably null
Transcript: ENSMUST00000173325

SMART Domains

Protein: ENSMUSP00000134443
Gene: ENSMUSG00000090165

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
Pfam:UDPGT	26	61	3.4e-10	PFAM
Pfam:UDPGT	59	210	8.9e-92	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.1%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010109A12Rik	A	G	5: 93,206,575		probably benign	Het
4930563M21Rik	C	T	9: 55,989,476	V283I	possibly damaging	Het
4933408B17Rik	T	A	18: 34,580,841	M244L	probably benign	Het
Acot11	C	T	4: 106,760,130	G240R	probably damaging	Het
Allc	T	A	12: 28,557,389	H288L	probably damaging	Het
Atg16l1	T	C	1: 87,774,854	I279T	possibly damaging	Het
Atp6v1b1	A	G	6: 83,751,875		probably benign	Het
Barhl1	G	A	2: 28,915,483	P66L	probably benign	Het
Cfap58	A	T	19: 47,955,417	D352V	probably benign	Het
Cyp3a44	T	A	5: 145,794,971	K122*	probably null	Het
Dnaic1	G	T	4: 41,625,308	R424L	probably benign	Het
Dync1li2	G	T	8: 104,440,535	H79Q	probably damaging	Het
Fem1b	A	G	9: 62,796,733	V415A	possibly damaging	Het
Fgb	C	T	3: 83,046,787	S48N	possibly damaging	Het
Gm5624	T	C	14: 44,561,875	D31G	possibly damaging	Het
Gm8909	A	T	17: 36,165,730	V284E	probably damaging	Het
Gzmn	T	A	14: 56,165,975	I226F	probably damaging	Het
Il31ra	T	C	13: 112,547,368	S184G	probably damaging	Het
Insrr	C	T	3: 87,813,566	R1044C	probably damaging	Het
Kcnj15	A	G	16: 95,296,334	S272G	probably damaging	Het
Kcnk16	C	T	14: 20,262,929	A106T	probably benign	Het
Kmt2b	A	G	7: 30,584,559	V876A	probably damaging	Het
Large2	G	T	2: 92,370,870		probably benign	Het
Maml2	A	T	9: 13,621,551		probably benign	Het
Me1	A	G	9: 86,582,798	L533P	possibly damaging	Het
Muc16	A	G	9: 18,566,690		probably null	Het
Nova2	C	A	7: 18,958,494	T516K	probably damaging	Het
Olfr365	T	A	2: 37,202,106	N288K	probably damaging	Het
Olfr657	T	C	7: 104,635,887	I71T	probably damaging	Het
Otogl	G	A	10: 107,777,117		silent	Het
Ppp2r1a	T	A	17: 20,955,825	V103E	probably benign	Het
Prl3d3	G	A	13: 27,157,164		probably null	Het
Rhbdf1	G	T	11: 32,214,042	A288E	possibly damaging	Het
Rnf213	T	C	11: 119,430,321	S1202P	possibly damaging	Het
Slc25a17	A	G	15: 81,337,953	V106A	probably benign	Het
Slc4a4	T	G	5: 89,170,765	S640A	probably benign	Het
Smc4	T	A	3: 69,016,772	Y298N	probably damaging	Het
Tek	G	T	4: 94,853,495	G830*	probably null	Het
Tgfb1	A	T	7: 25,689,162		probably benign	Het
Themis	T	C	10: 28,781,907	I157T	probably damaging	Het
Trmt12	A	G	15: 58,872,665		probably benign	Het
Trrap	T	C	5: 144,856,950	W3654R	probably damaging	Het
Tspan3	C	T	9: 56,147,440	G108S	probably damaging	Het
Vps13b	A	G	15: 35,770,683	K2091E	probably benign	Het
Wdr62	A	T	7: 30,271,620	V184D	probably damaging	Het
Zfp958	C	A	8: 4,628,247	Q90K	probably benign	Het

Other mutations in Ugt1a10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01126:Ugt1a10	APN	1	88055987	missense	possibly damaging	0.72
IGL02219:Ugt1a10	APN	1	88056058	missense	probably benign	0.00
IGL02511:Ugt1a10	APN	1	88055863	missense	probably damaging	1.00
IGL02990:Ugt1a10	APN	1	88055879	missense	probably damaging	1.00
PIT4142001:Ugt1a10	UTSW	1	88216158	small deletion	probably benign
R0201:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R0201:Ugt1a10	UTSW	1	88218249	missense	probably damaging	1.00
R0522:Ugt1a10	UTSW	1	88218249	missense	probably damaging	1.00
R0525:Ugt1a10	UTSW	1	88218249	missense	probably damaging	1.00
R0554:Ugt1a10	UTSW	1	88056095	missense	probably damaging	1.00
R0748:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R0811:Ugt1a10	UTSW	1	88056182	missense	probably benign	0.33
R0812:Ugt1a10	UTSW	1	88056182	missense	probably benign	0.33
R1129:Ugt1a10	UTSW	1	88055609	missense	probably benign
R1207:Ugt1a10	UTSW	1	88216254	missense	probably damaging	1.00
R1432:Ugt1a10	UTSW	1	88216260	missense	probably damaging	1.00
R1457:Ugt1a10	UTSW	1	88055711	missense	probably damaging	1.00
R1469:Ugt1a10	UTSW	1	88216254	missense	probably damaging	1.00
R1972:Ugt1a10	UTSW	1	88056047	missense	probably damaging	1.00
R1973:Ugt1a10	UTSW	1	88056047	missense	probably damaging	1.00
R2039:Ugt1a10	UTSW	1	88055981	missense	probably benign	0.32
R2307:Ugt1a10	UTSW	1	88055947	missense	probably benign	0.01
R3952:Ugt1a10	UTSW	1	88216140	missense	probably damaging	1.00
R3973:Ugt1a10	UTSW	1	88216140	missense	probably damaging	1.00
R4232:Ugt1a10	UTSW	1	88056210	missense	probably benign	0.39
R4392:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R4393:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R4402:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R4417:Ugt1a10	UTSW	1	88055995	missense	probably benign
R4474:Ugt1a10	UTSW	1	88215928	intron	probably benign
R4476:Ugt1a10	UTSW	1	88215928	intron	probably benign
R4515:Ugt1a10	UTSW	1	88056197	missense	probably damaging	1.00
R4579:Ugt1a10	UTSW	1	88056116	missense	probably benign
R4582:Ugt1a10	UTSW	1	88055741	missense	possibly damaging	0.90
R4609:Ugt1a10	UTSW	1	88055482	start codon destroyed	possibly damaging	0.92
R4627:Ugt1a10	UTSW	1	88218390	missense	probably damaging	1.00
R4790:Ugt1a10	UTSW	1	88056287	missense	probably damaging	0.98
R4799:Ugt1a10	UTSW	1	88215928	intron	probably benign
R4910:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R4915:Ugt1a10	UTSW	1	88055924	missense	probably damaging	1.00
R5110:Ugt1a10	UTSW	1	88056252	splice site	probably null
R5168:Ugt1a10	UTSW	1	88055809	missense	probably benign	0.01
R5329:Ugt1a10	UTSW	1	88216254	missense	probably damaging	1.00
R5373:Ugt1a10	UTSW	1	88055910	missense	probably damaging	0.98
R5374:Ugt1a10	UTSW	1	88055910	missense	probably damaging	0.98
R5615:Ugt1a10	UTSW	1	88216158	small deletion	probably benign
R6498:Ugt1a10	UTSW	1	88216140	missense	probably damaging	1.00
R6809:Ugt1a10	UTSW	1	88055925	missense	probably damaging	0.98
R6924:Ugt1a10	UTSW	1	88055657	missense	probably damaging	0.99
R6967:Ugt1a10	UTSW	1	88215123	missense	probably damaging	1.00
R7913:Ugt1a10	UTSW	1	88055755	missense	probably benign	0.00
R9165:Ugt1a10	UTSW	1	88055787	missense	probably benign	0.00
R9264:Ugt1a10	UTSW	1	88055671	missense	possibly damaging	0.62
R9475:Ugt1a10	UTSW	1	88216260	missense	probably damaging	1.00
S24628:Ugt1a10	UTSW	1	88216158	small deletion	probably benign
X0013:Ugt1a10	UTSW	1	88216254	missense	probably damaging	1.00
Z1088:Ugt1a10	UTSW	1	88055842	missense	probably benign	0.20
Z1190:Ugt1a10	UTSW	1	88216158	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- ACACCATGACTTTCAAGGAGAG -3'
(R):5'- AGTCACCTATATGGAATTCTTCTCC -3'

Sequencing Primer
(F):5'- CCATGACTTTCAAGGAGAGAGTGTG -3'
(R):5'- AGCTCTCCTCATGTGCTA -3'

Posted On

2018-08-01