Mutagenetix > Incidental Mutation

Incidental Mutation 'R6727:Zfp958'

529909

Institutional Source

Beutler Lab

Gene Symbol

Zfp958

Ensembl Gene

ENSMUSG00000058748

Gene Name

zinc finger protein 958

Synonyms

BC003267

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R6727 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

4613167-4630231 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 4628247 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Lysine at position 90 (Q90K)

Ref Sequence

ENSEMBL: ENSMUSP00000072937 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073201] [ENSMUST00000202692]

AlphaFold

Q99LG7

Predicted Effect

probably benign
Transcript: ENSMUST00000073201
AA Change: Q90K

PolyPhen 2 Score 0.078 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000072937
Gene: ENSMUSG00000058748
AA Change: Q90K

Domain	Start	End	E-Value	Type
KRAB	3	65	1.96e-17	SMART
ZnF_C2H2	102	124	2.67e-1	SMART
ZnF_C2H2	130	152	8.47e-4	SMART
ZnF_C2H2	158	180	5.5e-3	SMART
ZnF_C2H2	186	208	1.38e-3	SMART
ZnF_C2H2	214	236	9.96e-1	SMART
ZnF_C2H2	242	264	2.15e-5	SMART
ZnF_C2H2	270	292	3.16e-3	SMART
ZnF_C2H2	298	320	1.69e-3	SMART
ZnF_C2H2	326	348	5.14e-3	SMART
ZnF_C2H2	354	376	2.91e-2	SMART
ZnF_C2H2	382	404	2.27e-4	SMART
ZnF_C2H2	410	432	4.79e-3	SMART
ZnF_C2H2	438	460	6.88e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000202692
AA Change: Q91K

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000143842
Gene: ENSMUSG00000058748
AA Change: Q91K

Domain	Start	End	E-Value	Type
KRAB	4	66	1.96e-17	SMART
ZnF_C2H2	103	125	2.67e-1	SMART
ZnF_C2H2	131	153	8.47e-4	SMART
ZnF_C2H2	159	181	5.5e-3	SMART
ZnF_C2H2	187	209	1.38e-3	SMART
ZnF_C2H2	215	237	9.96e-1	SMART
ZnF_C2H2	243	265	2.15e-5	SMART
ZnF_C2H2	271	293	3.16e-3	SMART
ZnF_C2H2	299	321	1.69e-3	SMART
ZnF_C2H2	327	349	5.14e-3	SMART
ZnF_C2H2	355	377	2.91e-2	SMART
ZnF_C2H2	383	405	2.27e-4	SMART
ZnF_C2H2	411	433	4.79e-3	SMART
ZnF_C2H2	439	461	6.88e-4	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.1%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010109A12Rik	A	G	5: 93,206,575		probably benign	Het
4930563M21Rik	C	T	9: 55,989,476	V283I	possibly damaging	Het
4933408B17Rik	T	A	18: 34,580,841	M244L	probably benign	Het
Acot11	C	T	4: 106,760,130	G240R	probably damaging	Het
Allc	T	A	12: 28,557,389	H288L	probably damaging	Het
Atg16l1	T	C	1: 87,774,854	I279T	possibly damaging	Het
Atp6v1b1	A	G	6: 83,751,875		probably benign	Het
Barhl1	G	A	2: 28,915,483	P66L	probably benign	Het
Cfap58	A	T	19: 47,955,417	D352V	probably benign	Het
Cyp3a44	T	A	5: 145,794,971	K122*	probably null	Het
Dnaic1	G	T	4: 41,625,308	R424L	probably benign	Het
Dync1li2	G	T	8: 104,440,535	H79Q	probably damaging	Het
Fem1b	A	G	9: 62,796,733	V415A	possibly damaging	Het
Fgb	C	T	3: 83,046,787	S48N	possibly damaging	Het
Gm5624	T	C	14: 44,561,875	D31G	possibly damaging	Het
Gm8909	A	T	17: 36,165,730	V284E	probably damaging	Het
Gzmn	T	A	14: 56,165,975	I226F	probably damaging	Het
Il31ra	T	C	13: 112,547,368	S184G	probably damaging	Het
Insrr	C	T	3: 87,813,566	R1044C	probably damaging	Het
Kcnj15	A	G	16: 95,296,334	S272G	probably damaging	Het
Kcnk16	C	T	14: 20,262,929	A106T	probably benign	Het
Kmt2b	A	G	7: 30,584,559	V876A	probably damaging	Het
Large2	G	T	2: 92,370,870		probably benign	Het
Maml2	A	T	9: 13,621,551		probably benign	Het
Me1	A	G	9: 86,582,798	L533P	possibly damaging	Het
Muc16	A	G	9: 18,566,690		probably null	Het
Nova2	C	A	7: 18,958,494	T516K	probably damaging	Het
Olfr365	T	A	2: 37,202,106	N288K	probably damaging	Het
Olfr657	T	C	7: 104,635,887	I71T	probably damaging	Het
Otogl	G	A	10: 107,777,117		silent	Het
Ppp2r1a	T	A	17: 20,955,825	V103E	probably benign	Het
Prl3d3	G	A	13: 27,157,164		probably null	Het
Rhbdf1	G	T	11: 32,214,042	A288E	possibly damaging	Het
Rnf213	T	C	11: 119,430,321	S1202P	possibly damaging	Het
Slc25a17	A	G	15: 81,337,953	V106A	probably benign	Het
Slc4a4	T	G	5: 89,170,765	S640A	probably benign	Het
Smc4	T	A	3: 69,016,772	Y298N	probably damaging	Het
Tek	G	T	4: 94,853,495	G830*	probably null	Het
Tgfb1	A	T	7: 25,689,162		probably benign	Het
Themis	T	C	10: 28,781,907	I157T	probably damaging	Het
Trmt12	A	G	15: 58,872,665		probably benign	Het
Trrap	T	C	5: 144,856,950	W3654R	probably damaging	Het
Tspan3	C	T	9: 56,147,440	G108S	probably damaging	Het
Ugt1a10	T	A	1: 88,056,257		probably null	Het
Vps13b	A	G	15: 35,770,683	K2091E	probably benign	Het
Wdr62	A	T	7: 30,271,620	V184D	probably damaging	Het

Other mutations in Zfp958

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01950:Zfp958	APN	8	4628917	missense	probably damaging	1.00
IGL02185:Zfp958	APN	8	4628990	nonsense	probably null
IGL02716:Zfp958	APN	8	4625967	critical splice donor site	probably null
R1118:Zfp958	UTSW	8	4626169	missense	possibly damaging	0.50
R1119:Zfp958	UTSW	8	4626169	missense	possibly damaging	0.50
R1478:Zfp958	UTSW	8	4629190	missense	probably damaging	1.00
R1816:Zfp958	UTSW	8	4629147	missense	possibly damaging	0.89
R1837:Zfp958	UTSW	8	4628590	missense	probably damaging	1.00
R1838:Zfp958	UTSW	8	4628590	missense	probably damaging	1.00
R3763:Zfp958	UTSW	8	4626226	critical splice donor site	probably null
R5257:Zfp958	UTSW	8	4628456	missense	probably benign	0.28
R5258:Zfp958	UTSW	8	4628456	missense	probably benign	0.28
R5304:Zfp958	UTSW	8	4626196	missense	possibly damaging	0.73
R5512:Zfp958	UTSW	8	4625838	critical splice acceptor site	probably null
R5907:Zfp958	UTSW	8	4629072	missense	probably benign	0.01
R6604:Zfp958	UTSW	8	4628245	missense	probably damaging	1.00
R6688:Zfp958	UTSW	8	4628940	missense	possibly damaging	0.91
R6981:Zfp958	UTSW	8	4626170	missense	probably benign	0.00
R7010:Zfp958	UTSW	8	4628377	missense	probably benign	0.13
R7140:Zfp958	UTSW	8	4628481	missense	probably benign	0.05
R8096:Zfp958	UTSW	8	4613273	start gained	probably benign
R8491:Zfp958	UTSW	8	4626215	missense	probably damaging	0.99
R8673:Zfp958	UTSW	8	4628268	missense	possibly damaging	0.54
R8847:Zfp958	UTSW	8	4628434	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCAGTGTGGTAAGGTTCTAAGTT -3'
(R):5'- TATGATGTGCAAAGGCTTTACC -3'

Sequencing Primer
(F):5'- GGTATCTTCAAAGATGCAAAGCAAC -3'
(R):5'- GTGCAAAGGCTTTACCACATTG -3'

Posted On

2018-08-01