Mutagenetix > Incidental Mutation

Incidental Mutation 'R6727:Zfp958'

529909

Institutional Source

Beutler Lab

Gene Symbol

Zfp958

Ensembl Gene

ENSMUSG00000058748

Gene Name

zinc finger protein 958

Synonyms

BC003267

MMRRC Submission

044845-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.084) question?

Stock #

R6727 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

4663167-4680231 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 4678247 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Lysine at position 90 (Q90K)

Ref Sequence

ENSEMBL: ENSMUSP00000072937 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073201] [ENSMUST00000202692]

AlphaFold

Q99LG7

Predicted Effect

probably benign
Transcript: ENSMUST00000073201
AA Change: Q90K

PolyPhen 2 Score 0.078 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000072937
Gene: ENSMUSG00000058748
AA Change: Q90K

Domain	Start	End	E-Value	Type
KRAB	3	65	1.96e-17	SMART
ZnF_C2H2	102	124	2.67e-1	SMART
ZnF_C2H2	130	152	8.47e-4	SMART
ZnF_C2H2	158	180	5.5e-3	SMART
ZnF_C2H2	186	208	1.38e-3	SMART
ZnF_C2H2	214	236	9.96e-1	SMART
ZnF_C2H2	242	264	2.15e-5	SMART
ZnF_C2H2	270	292	3.16e-3	SMART
ZnF_C2H2	298	320	1.69e-3	SMART
ZnF_C2H2	326	348	5.14e-3	SMART
ZnF_C2H2	354	376	2.91e-2	SMART
ZnF_C2H2	382	404	2.27e-4	SMART
ZnF_C2H2	410	432	4.79e-3	SMART
ZnF_C2H2	438	460	6.88e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000202692
AA Change: Q91K

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000143842
Gene: ENSMUSG00000058748
AA Change: Q91K

Domain	Start	End	E-Value	Type
KRAB	4	66	1.96e-17	SMART
ZnF_C2H2	103	125	2.67e-1	SMART
ZnF_C2H2	131	153	8.47e-4	SMART
ZnF_C2H2	159	181	5.5e-3	SMART
ZnF_C2H2	187	209	1.38e-3	SMART
ZnF_C2H2	215	237	9.96e-1	SMART
ZnF_C2H2	243	265	2.15e-5	SMART
ZnF_C2H2	271	293	3.16e-3	SMART
ZnF_C2H2	299	321	1.69e-3	SMART
ZnF_C2H2	327	349	5.14e-3	SMART
ZnF_C2H2	355	377	2.91e-2	SMART
ZnF_C2H2	383	405	2.27e-4	SMART
ZnF_C2H2	411	433	4.79e-3	SMART
ZnF_C2H2	439	461	6.88e-4	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.1%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010109A12Rik	A	G	5: 93,354,434 (GRCm39)		probably benign	Het
4930563M21Rik	C	T	9: 55,896,760 (GRCm39)	V283I	possibly damaging	Het
Acot11	C	T	4: 106,617,327 (GRCm39)	G240R	probably damaging	Het
Allc	T	A	12: 28,607,388 (GRCm39)	H288L	probably damaging	Het
Atg16l1	T	C	1: 87,702,576 (GRCm39)	I279T	possibly damaging	Het
Atp6v1b1	A	G	6: 83,728,857 (GRCm39)		probably benign	Het
Barhl1	G	A	2: 28,805,495 (GRCm39)	P66L	probably benign	Het
Brd8dc	T	A	18: 34,713,894 (GRCm39)	M244L	probably benign	Het
Cfap58	A	T	19: 47,943,856 (GRCm39)	D352V	probably benign	Het
Cyp3a44	T	A	5: 145,731,781 (GRCm39)	K122*	probably null	Het
Dnai1	G	T	4: 41,625,308 (GRCm39)	R424L	probably benign	Het
Dync1li2	G	T	8: 105,167,167 (GRCm39)	H79Q	probably damaging	Het
Fem1b	A	G	9: 62,704,015 (GRCm39)	V415A	possibly damaging	Het
Fgb	C	T	3: 82,954,094 (GRCm39)	S48N	possibly damaging	Het
Gm5624	T	C	14: 44,799,332 (GRCm39)	D31G	possibly damaging	Het
Gzmn	T	A	14: 56,403,432 (GRCm39)	I226F	probably damaging	Het
H2-T5	A	T	17: 36,476,622 (GRCm39)	V284E	probably damaging	Het
Il31ra	T	C	13: 112,683,902 (GRCm39)	S184G	probably damaging	Het
Insrr	C	T	3: 87,720,873 (GRCm39)	R1044C	probably damaging	Het
Kcnj15	A	G	16: 95,097,193 (GRCm39)	S272G	probably damaging	Het
Kcnk16	C	T	14: 20,312,997 (GRCm39)	A106T	probably benign	Het
Kmt2b	A	G	7: 30,283,984 (GRCm39)	V876A	probably damaging	Het
Large2	G	T	2: 92,201,215 (GRCm39)		probably benign	Het
Maml2	A	T	9: 13,532,847 (GRCm39)		probably benign	Het
Me1	A	G	9: 86,464,851 (GRCm39)	L533P	possibly damaging	Het
Muc16	A	G	9: 18,477,986 (GRCm39)		probably null	Het
Nova2	C	A	7: 18,692,419 (GRCm39)	T516K	probably damaging	Het
Or1l4	T	A	2: 37,092,118 (GRCm39)	N288K	probably damaging	Het
Or56b1	T	C	7: 104,285,094 (GRCm39)	I71T	probably damaging	Het
Otogl	G	A	10: 107,612,978 (GRCm39)		silent	Het
Ppp2r1a	T	A	17: 21,176,087 (GRCm39)	V103E	probably benign	Het
Prl3d3	G	A	13: 27,341,147 (GRCm39)		probably null	Het
Rhbdf1	G	T	11: 32,164,042 (GRCm39)	A288E	possibly damaging	Het
Rnf213	T	C	11: 119,321,147 (GRCm39)	S1202P	possibly damaging	Het
Slc25a17	A	G	15: 81,222,154 (GRCm39)	V106A	probably benign	Het
Slc4a4	T	G	5: 89,318,624 (GRCm39)	S640A	probably benign	Het
Smc4	T	A	3: 68,924,105 (GRCm39)	Y298N	probably damaging	Het
Tek	G	T	4: 94,741,732 (GRCm39)	G830*	probably null	Het
Tgfb1	A	T	7: 25,388,587 (GRCm39)		probably benign	Het
Themis	T	C	10: 28,657,903 (GRCm39)	I157T	probably damaging	Het
Trmt12	A	G	15: 58,744,514 (GRCm39)		probably benign	Het
Trrap	T	C	5: 144,793,760 (GRCm39)	W3654R	probably damaging	Het
Tspan3	C	T	9: 56,054,724 (GRCm39)	G108S	probably damaging	Het
Ugt1a10	T	A	1: 87,983,979 (GRCm39)		probably null	Het
Vps13b	A	G	15: 35,770,829 (GRCm39)	K2091E	probably benign	Het
Wdr62	A	T	7: 29,971,045 (GRCm39)	V184D	probably damaging	Het

Other mutations in Zfp958

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01950:Zfp958	APN	8	4,678,917 (GRCm39)	missense	probably damaging	1.00
IGL02185:Zfp958	APN	8	4,678,990 (GRCm39)	nonsense	probably null
IGL02716:Zfp958	APN	8	4,675,967 (GRCm39)	critical splice donor site	probably null
R1118:Zfp958	UTSW	8	4,676,169 (GRCm39)	missense	possibly damaging	0.50
R1119:Zfp958	UTSW	8	4,676,169 (GRCm39)	missense	possibly damaging	0.50
R1478:Zfp958	UTSW	8	4,679,190 (GRCm39)	missense	probably damaging	1.00
R1816:Zfp958	UTSW	8	4,679,147 (GRCm39)	missense	possibly damaging	0.89
R1837:Zfp958	UTSW	8	4,678,590 (GRCm39)	missense	probably damaging	1.00
R1838:Zfp958	UTSW	8	4,678,590 (GRCm39)	missense	probably damaging	1.00
R3763:Zfp958	UTSW	8	4,676,226 (GRCm39)	critical splice donor site	probably null
R5257:Zfp958	UTSW	8	4,678,456 (GRCm39)	missense	probably benign	0.28
R5258:Zfp958	UTSW	8	4,678,456 (GRCm39)	missense	probably benign	0.28
R5304:Zfp958	UTSW	8	4,676,196 (GRCm39)	missense	possibly damaging	0.73
R5512:Zfp958	UTSW	8	4,675,838 (GRCm39)	critical splice acceptor site	probably null
R5907:Zfp958	UTSW	8	4,679,072 (GRCm39)	missense	probably benign	0.01
R6604:Zfp958	UTSW	8	4,678,245 (GRCm39)	missense	probably damaging	1.00
R6688:Zfp958	UTSW	8	4,678,940 (GRCm39)	missense	possibly damaging	0.91
R6981:Zfp958	UTSW	8	4,676,170 (GRCm39)	missense	probably benign	0.00
R7010:Zfp958	UTSW	8	4,678,377 (GRCm39)	missense	probably benign	0.13
R7140:Zfp958	UTSW	8	4,678,481 (GRCm39)	missense	probably benign	0.05
R8096:Zfp958	UTSW	8	4,663,273 (GRCm39)	start gained	probably benign
R8491:Zfp958	UTSW	8	4,676,215 (GRCm39)	missense	probably damaging	0.99
R8673:Zfp958	UTSW	8	4,678,268 (GRCm39)	missense	possibly damaging	0.54
R8847:Zfp958	UTSW	8	4,678,434 (GRCm39)	missense	probably damaging	1.00
R9612:Zfp958	UTSW	8	4,678,298 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCAGTGTGGTAAGGTTCTAAGTT -3'
(R):5'- TATGATGTGCAAAGGCTTTACC -3'

Sequencing Primer
(F):5'- GGTATCTTCAAAGATGCAAAGCAAC -3'
(R):5'- GTGCAAAGGCTTTACCACATTG -3'

Posted On

2018-08-01