Mutagenetix > Incidental Mutations

Incidental Mutation 'R6732:Mroh7'

530100

Institutional Source

Beutler Lab

Gene Symbol

Mroh7

Ensembl Gene

ENSMUSG00000047502

Gene Name

maestro heat-like repeat family member 7

Synonyms

Heatr8, LOC381538, Gm1027

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6732 (G1)

Quality Score

130.467

Status

Validated

Chromosome

Chromosomal Location

106680417-106730925 bp(-) (GRCm38)

Type of Mutation

frame shift

DNA Base Change (assembly)

GTT to GTTT at 106680713 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000102382 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026480] [ENSMUST00000106770] [ENSMUST00000106772] [ENSMUST00000135676]

Predicted Effect

probably benign
Transcript: ENSMUST00000026480

SMART Domains

Protein: ENSMUSP00000026480
Gene: ENSMUSG00000025413

Domain	Start	End	E-Value	Type
TPR	79	112	1.26e1	SMART
TPR	117	150	7.27e0	SMART
TPR	151	184	3.07e1	SMART
low complexity region	235	246	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106770

SMART Domains

Protein: ENSMUSP00000102382
Gene: ENSMUSG00000047502

Domain	Start	End	E-Value	Type
low complexity region	39	61	N/A	INTRINSIC
low complexity region	318	332	N/A	INTRINSIC
low complexity region	563	573	N/A	INTRINSIC
SCOP:d1b3ua_	634	1218	6e-9	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000106772

SMART Domains

Protein: ENSMUSP00000102384
Gene: ENSMUSG00000025413

Domain	Start	End	E-Value	Type
TPR	79	112	1.26e1	SMART
TPR	117	150	7.27e0	SMART
TPR	151	184	3.07e1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127571

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132572

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134160

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135000

Predicted Effect

probably benign
Transcript: ENSMUST00000135676

SMART Domains

Protein: ENSMUSP00000116620
Gene: ENSMUSG00000025413

Domain	Start	End	E-Value	Type
Pfam:TPR_11	77	148	1.1e-14	PFAM
Pfam:TPR_1	79	109	8.2e-5	PFAM
Pfam:TPR_2	79	110	1.2e-3	PFAM
Blast:TPR	173	203	1e-12	BLAST

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142342

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145374

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.5%

Validation Efficiency

98% (43/44)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930402H24Rik	A	G	2: 130,810,820		probably null	Het
Abcb11	A	G	2: 69,286,846	I486T	probably damaging	Het
Abcc5	T	C	16: 20,404,684	N158S	probably benign	Het
AI182371	G	T	2: 35,084,705		probably benign	Het
Ap1ar	G	A	3: 127,815,685	Q96*	probably null	Het
Cd101	C	T	3: 101,008,199	S684N	probably benign	Het
Cdh16	T	G	8: 104,618,533	I375L	probably benign	Het
Chchd6	A	G	6: 89,574,454	V75A	probably benign	Het
Coro2a	A	T	4: 46,551,374	N110K	probably damaging	Het
Cyp2c69	A	G	19: 39,881,499	V93A	probably benign	Het
Egln3	C	T	12: 54,180,641	A235T	probably benign	Het
Fryl	T	C	5: 73,054,781	T2335A	probably damaging	Het
Fzd3	T	C	14: 65,235,803	D172G	probably benign	Het
Galnt2	T	A	8: 124,340,822	W447R	probably damaging	Het
Iqce	A	G	5: 140,675,235	L450P	probably benign	Het
Ly9	T	C	1: 171,594,085	T533A	possibly damaging	Het
Map3k19	A	C	1: 127,824,232	F257V	probably benign	Het
Olfr202	A	T	16: 59,283,951	V182E	probably benign	Het
Pcdhb11	A	T	18: 37,422,144	I176F	probably benign	Het
Pigp	A	C	16: 94,365,441	L96R	probably damaging	Het
Pkd1	A	G	17: 24,569,413	D715G	probably damaging	Het
Plxnd1	A	C	6: 115,969,929	L828R	possibly damaging	Het
Pramef20	C	T	4: 144,373,173	V341I	probably benign	Het
Psmd2	T	A	16: 20,662,636	S814T	probably benign	Het
Pusl1	A	G	4: 155,891,116	S87P	probably benign	Het
Rgs3	T	A	4: 62,602,943	D34E	probably benign	Het
Rhof	A	G	5: 123,131,936	F53L	probably damaging	Het
Sik3	C	A	9: 46,212,553	P1217T	probably benign	Het
Skiv2l	G	A	17: 34,845,190	R507*	probably null	Het
Slc14a2	A	G	18: 78,192,174	Y263H	probably damaging	Het
Slc26a4	C	T	12: 31,526,600		probably null	Het
Smyd3	T	G	1: 179,395,830	H178P	probably benign	Het
Thada	T	C	17: 84,454,414		probably null	Het
Top1mt	A	C	15: 75,669,488		probably null	Het
Trim17	A	T	11: 58,971,025		probably null	Het
Ttn	A	T	2: 76,940,051	F2599I	possibly damaging	Het
Tuba3a	A	T	6: 125,281,645	D127E	probably benign	Het
Ucp1	A	G	8: 83,291,477	T68A	probably benign	Het
Vmn2r69	A	G	7: 85,411,143	V411A	probably benign	Het
Vmn2r74	A	T	7: 85,957,550	V196E	probably damaging	Het
Wdr59	T	C	8: 111,501,052	Y131C	probably damaging	Het
Yod1	G	A	1: 130,717,538	G19S	probably damaging	Het
Zbtb21	G	T	16: 97,951,082	S667Y	probably damaging	Het

Other mutations in Mroh7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01722:Mroh7	APN	4	106703161	missense	probably benign	0.00
IGL01729:Mroh7	APN	4	106704205	missense	possibly damaging	0.66
IGL01834:Mroh7	APN	4	106680874	missense	probably benign	0.00
IGL02003:Mroh7	APN	4	106702529	missense	probably damaging	0.96
IGL02135:Mroh7	APN	4	106702510	missense	probably damaging	1.00
IGL02335:Mroh7	APN	4	106707782	missense	probably damaging	1.00
IGL02532:Mroh7	APN	4	106720591	missense	probably benign	0.04
IGL02896:Mroh7	APN	4	106699816	missense	possibly damaging	0.94
IGL03066:Mroh7	APN	4	106692398	missense	possibly damaging	0.85
IGL03298:Mroh7	APN	4	106714091	nonsense	probably null
holy	UTSW	4	106709955	splice site	probably null
moley	UTSW	4	106694312	splice site	probably null
P0016:Mroh7	UTSW	4	106707857	critical splice acceptor site	probably null
R0019:Mroh7	UTSW	4	106721426	missense	probably benign	0.07
R0094:Mroh7	UTSW	4	106703184	missense	probably damaging	0.98
R0105:Mroh7	UTSW	4	106711270	missense	possibly damaging	0.49
R0105:Mroh7	UTSW	4	106711270	missense	possibly damaging	0.49
R0515:Mroh7	UTSW	4	106691664	missense	probably benign	0.01
R0828:Mroh7	UTSW	4	106699876	missense	probably damaging	0.99
R0831:Mroh7	UTSW	4	106680793	missense	possibly damaging	0.92
R1107:Mroh7	UTSW	4	106707594	unclassified	probably null
R1301:Mroh7	UTSW	4	106720495	missense	probably damaging	0.99
R1456:Mroh7	UTSW	4	106695141	splice site	probably benign
R1491:Mroh7	UTSW	4	106703058	missense	probably benign	0.11
R1540:Mroh7	UTSW	4	106703076	missense	probably benign	0.11
R1560:Mroh7	UTSW	4	106711254	missense	possibly damaging	0.78
R1645:Mroh7	UTSW	4	106720668	missense	probably benign	0.19
R1804:Mroh7	UTSW	4	106694392	missense	possibly damaging	0.76
R2162:Mroh7	UTSW	4	106700181	missense	probably damaging	0.96
R2265:Mroh7	UTSW	4	106720927	missense	probably benign	0.01
R2866:Mroh7	UTSW	4	106691090	missense	probably damaging	1.00
R3716:Mroh7	UTSW	4	106704210	missense	probably benign	0.25
R3718:Mroh7	UTSW	4	106704210	missense	probably benign	0.25
R4530:Mroh7	UTSW	4	106720437	missense	possibly damaging	0.71
R4661:Mroh7	UTSW	4	106691513	critical splice donor site	probably null
R4706:Mroh7	UTSW	4	106691624	missense	possibly damaging	0.86
R4910:Mroh7	UTSW	4	106709955	splice site	probably null
R4965:Mroh7	UTSW	4	106690987	missense	possibly damaging	0.77
R4969:Mroh7	UTSW	4	106680873	missense	probably benign
R4971:Mroh7	UTSW	4	106691552	missense	probably benign	0.04
R5083:Mroh7	UTSW	4	106690318	missense	probably benign	0.03
R5207:Mroh7	UTSW	4	106721386	missense	probably damaging	0.97
R5364:Mroh7	UTSW	4	106691643	missense	probably benign	0.10
R5392:Mroh7	UTSW	4	106711251	critical splice donor site	probably null
R5630:Mroh7	UTSW	4	106720567	missense	possibly damaging	0.71
R5691:Mroh7	UTSW	4	106702618	missense	probably damaging	0.96
R5703:Mroh7	UTSW	4	106708560	missense	possibly damaging	0.77
R5707:Mroh7	UTSW	4	106681885	missense	possibly damaging	0.73
R5919:Mroh7	UTSW	4	106694312	splice site	probably null
R5979:Mroh7	UTSW	4	106720926	missense	probably benign	0.00
R6479:Mroh7	UTSW	4	106703188	missense	possibly damaging	0.75
R6520:Mroh7	UTSW	4	106721263	missense	probably benign	0.00
R6657:Mroh7	UTSW	4	106702500	nonsense	probably null
R6817:Mroh7	UTSW	4	106714115	missense	probably benign	0.00
R6980:Mroh7	UTSW	4	106700237	missense	probably benign	0.05
R7062:Mroh7	UTSW	4	106683980	missense	probably damaging	1.00
R7116:Mroh7	UTSW	4	106711320	missense	probably benign	0.07
R7134:Mroh7	UTSW	4	106720594	missense	probably damaging	0.99
R7169:Mroh7	UTSW	4	106691639	missense	probably damaging	0.99
R7419:Mroh7	UTSW	4	106683918	missense	probably benign
R7516:Mroh7	UTSW	4	106691119	missense	probably benign	0.00
R7525:Mroh7	UTSW	4	106709702	missense	probably benign	0.22
R7540:Mroh7	UTSW	4	106720398	missense	possibly damaging	0.85
R7849:Mroh7	UTSW	4	106721090	missense	probably benign
R7932:Mroh7	UTSW	4	106721090	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACTTGTCAGAGTGAGGGCTC -3'
(R):5'- TCTGGAGATCTTGAGACACGAC -3'

Sequencing Primer
(F):5'- AGAGTGAGGGCTCCGCAG -3'
(R):5'- CCCAGAAGCCTCCGTGTGTATC -3'

Posted On

2018-08-01