Incidental Mutation 'R6733:Il25'
ID 530160
Institutional Source Beutler Lab
Gene Symbol Il25
Ensembl Gene ENSMUSG00000040770
Gene Name interleukin 25
Synonyms Il17e, IL-17E
MMRRC Submission 044851-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R6733 (G1)
Quality Score 225.009
Status Not validated
Chromosome 14
Chromosomal Location 55170152-55173294 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 55170490 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Threonine at position 21 (I21T)
Ref Sequence ENSEMBL: ENSMUSP00000039641 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000037814] [ENSMUST00000037863] [ENSMUST00000227441]
AlphaFold Q8VHH8
Predicted Effect probably benign
Transcript: ENSMUST00000037814
SMART Domains Protein: ENSMUSP00000036138
Gene: ENSMUSG00000040759

DomainStartEndE-ValueType
transmembrane domain 35 56 N/A INTRINSIC
transmembrane domain 60 82 N/A INTRINSIC
transmembrane domain 95 114 N/A INTRINSIC
transmembrane domain 119 136 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000037863
AA Change: I21T

PolyPhen 2 Score 0.067 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000039641
Gene: ENSMUSG00000040770
AA Change: I21T

DomainStartEndE-ValueType
signal peptide 1 16 N/A INTRINSIC
Pfam:IL17 77 163 4.2e-23 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000226807
Predicted Effect probably benign
Transcript: ENSMUST00000227441
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.6%
  • 10x: 98.3%
  • 20x: 95.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
PHENOTYPE: Mice homozygous for a null allele have increased susceptibility to parasitic infection. Male homozygous mutant mice exhibit notably increased mean serum triglyceride levels in another null allele. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 32 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Afdn C T 17: 14,043,615 (GRCm39) H358Y probably benign Het
Aoc1l2 T C 6: 48,907,464 (GRCm39) S155P probably damaging Het
Ccdc125 T A 13: 100,830,995 (GRCm39) M394K probably benign Het
Cfd C A 10: 79,727,636 (GRCm39) H103Q probably damaging Het
Cnot2 G A 10: 116,334,058 (GRCm39) P371S possibly damaging Het
Dedd2 T C 7: 24,903,332 (GRCm39) E209G probably benign Het
Dnah3 A G 7: 119,522,197 (GRCm39) S3999P probably benign Het
Fer1l5 T C 1: 36,447,753 (GRCm39) probably null Het
H6pd A T 4: 150,069,578 (GRCm39) probably null Het
Kmt2c C T 5: 25,614,291 (GRCm39) S143N probably damaging Het
Marveld3 T C 8: 110,688,681 (GRCm39) D20G possibly damaging Het
Msl1 A G 11: 98,690,882 (GRCm39) E122G probably damaging Het
Obscn A T 11: 58,919,421 (GRCm39) V6861E probably damaging Het
Or5b97 A T 19: 12,878,605 (GRCm39) C180S probably damaging Het
Phc1 A T 6: 122,313,845 (GRCm39) M29K possibly damaging Het
Pkd1l3 T A 8: 110,375,126 (GRCm39) probably null Het
Prl5a1 G T 13: 28,333,919 (GRCm39) V141F possibly damaging Het
Psg20 A T 7: 18,408,547 (GRCm39) V391D probably damaging Het
Ptprh T C 7: 4,606,043 (GRCm39) probably null Het
Rasa3 T C 8: 13,630,037 (GRCm39) E580G possibly damaging Het
Ror1 T C 4: 100,283,252 (GRCm39) V439A probably benign Het
Rsl1 A G 13: 67,325,206 (GRCm39) T81A probably benign Het
Sgpp1 G C 12: 75,782,243 (GRCm39) P32R probably benign Het
Slc22a8 T C 19: 8,586,656 (GRCm39) L389P probably benign Het
Slc6a11 A G 6: 114,111,859 (GRCm39) Y142C probably damaging Het
Syt9 C T 7: 107,024,503 (GRCm39) T132I probably damaging Het
Thop1 T A 10: 80,917,246 (GRCm39) I583N probably damaging Het
Tom1l1 A G 11: 90,575,886 (GRCm39) probably null Het
Unk A G 11: 115,941,581 (GRCm39) D276G probably damaging Het
Zfp942 C A 17: 22,147,733 (GRCm39) E299* probably null Het
Zkscan6 A G 11: 65,719,461 (GRCm39) T494A probably damaging Het
Zscan25 T A 5: 145,227,723 (GRCm39) probably null Het
Other mutations in Il25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03327:Il25 APN 14 55,172,817 (GRCm39) unclassified probably benign
R0035:Il25 UTSW 14 55,170,553 (GRCm39) missense probably damaging 0.99
R0368:Il25 UTSW 14 55,172,631 (GRCm39) splice site probably null
R1212:Il25 UTSW 14 55,170,212 (GRCm39) unclassified probably benign
R7044:Il25 UTSW 14 55,170,615 (GRCm39) missense probably benign
R7464:Il25 UTSW 14 55,170,679 (GRCm39) missense probably null 1.00
R7488:Il25 UTSW 14 55,170,459 (GRCm39) missense probably benign
Z1177:Il25 UTSW 14 55,172,664 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GTCTGACAGAGGAATGGCTG -3'
(R):5'- TAGCTCCAAGGAGAGATGGC -3'

Sequencing Primer
(F):5'- AAGGCTAGGCTTCCAGGC -3'
(R):5'- AGAGATGGCCCTGCTGTTGAG -3'
Posted On 2018-08-01