Mutagenetix > Incidental Mutation

Incidental Mutation 'R6742:Zfp935'

530504

Institutional Source

Beutler Lab

Gene Symbol

Zfp935

Ensembl Gene

ENSMUSG00000055228

Gene Name

zinc finger protein 935

Synonyms

8430426H19Rik

MMRRC Submission

044859-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R6742 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

62600830-62614626 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 62602293 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 302 (K302N)

Ref Sequence

ENSEMBL: ENSMUSP00000152662 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076195] [ENSMUST00000221747] [ENSMUST00000223247]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000076195

SMART Domains

Protein: ENSMUSP00000075552
Gene: ENSMUSG00000055228

Domain	Start	End	E-Value	Type
KRAB	4	66	8.26e-16	SMART
ZnF_C2H2	133	155	2.09e-3	SMART
ZnF_C2H2	161	183	1.4e-4	SMART
ZnF_C2H2	189	211	2.57e-3	SMART
ZnF_C2H2	217	239	5.5e-3	SMART
ZnF_C2H2	245	267	7.26e-3	SMART
ZnF_C2H2	273	295	1.4e-4	SMART
ZnF_C2H2	301	323	8.6e-5	SMART
ZnF_C2H2	329	351	1.2e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221260

Predicted Effect

probably damaging
Transcript: ENSMUST00000221747
AA Change: K302N

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221951

Predicted Effect

probably benign
Transcript: ENSMUST00000223247

Meta Mutation Damage Score

0.6329

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.6%

Validation Efficiency

100% (46/46)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	A	G	6: 121,654,995 (GRCm39)	R1440G	probably benign	Het
Abca13	T	A	11: 9,278,168 (GRCm39)	L3116Q	probably damaging	Het
Adgb	G	A	10: 10,287,593 (GRCm39)	H55Y	probably damaging	Het
AI182371	G	T	2: 34,974,717 (GRCm39)		probably benign	Het
Ank3	T	C	10: 69,827,412 (GRCm39)		probably benign	Het
Ccdc127	G	T	13: 74,501,042 (GRCm39)	G20W	probably damaging	Het
Ccdc149	G	A	5: 52,562,475 (GRCm39)	Q184*	probably null	Het
Cntrob	G	A	11: 69,213,749 (GRCm39)	P14S	probably damaging	Het
Dnah11	T	C	12: 118,077,629 (GRCm39)	E1288G	possibly damaging	Het
Eml2	G	A	7: 18,935,088 (GRCm39)	V432I	probably damaging	Het
Evpl	T	C	11: 116,113,640 (GRCm39)	D1350G	possibly damaging	Het
Fasn	T	C	11: 120,701,279 (GRCm39)	T1990A	probably damaging	Het
Itga2	T	A	13: 114,973,061 (GRCm39)	N1166I	possibly damaging	Het
Lmtk2	T	A	5: 144,085,175 (GRCm39)	C216S	probably damaging	Het
Lrp1b	T	C	2: 41,361,132 (GRCm39)	D557G	probably benign	Het
Lrrc32	T	C	7: 98,148,039 (GRCm39)	V273A	probably benign	Het
Nlrp4f	C	T	13: 65,335,254 (GRCm39)		probably null	Het
Ntng2	T	G	2: 29,090,940 (GRCm39)	M360L	probably benign	Het
Pik3c2b	A	G	1: 133,003,559 (GRCm39)	S504G	probably benign	Het
Ppfia4	A	C	1: 134,256,909 (GRCm39)	L104R	probably damaging	Het
Raph1	A	G	1: 60,564,879 (GRCm39)	S203P	probably damaging	Het
Rbl1	G	A	2: 157,011,918 (GRCm39)	T679I	probably benign	Het
Rnase4	G	T	14: 51,342,486 (GRCm39)	R70L	probably benign	Het
Rnf146	A	C	10: 29,223,528 (GRCm39)	D119E	probably damaging	Het
Rrp1b	A	G	17: 32,275,908 (GRCm39)	H485R	probably benign	Het
Rwdd4a	T	A	8: 48,000,998 (GRCm39)		probably null	Het
Scarf2	G	A	16: 17,624,351 (GRCm39)	C552Y	probably damaging	Het
Skic2	G	A	17: 35,064,166 (GRCm39)	R507*	probably null	Het
Sox10	T	C	15: 79,040,676 (GRCm39)	N127S	probably damaging	Het
Speer4a1	A	T	5: 26,241,054 (GRCm39)		probably null	Het
Tars1	A	T	15: 11,394,427 (GRCm39)	I70N	probably damaging	Het
Thsd7a	C	T	6: 12,408,815 (GRCm39)	V736M	probably damaging	Het
Timp3	G	A	10: 86,136,742 (GRCm39)	V9M	probably benign	Het
Tnfrsf1a	A	T	6: 125,333,911 (GRCm39)	N55Y	probably damaging	Het
Trim43a	G	A	9: 88,470,399 (GRCm39)	V402I	possibly damaging	Het
Tshz2	A	G	2: 169,725,677 (GRCm39)	D91G	probably damaging	Het
Ubac1	T	C	2: 25,895,418 (GRCm39)	D345G	possibly damaging	Het
Usp4	G	A	9: 108,251,438 (GRCm39)	V538I	possibly damaging	Het
Vmn2r19	A	T	6: 123,306,917 (GRCm39)	Y475F	possibly damaging	Het
Vmn2r87	A	T	10: 130,308,396 (GRCm39)	V614E	probably damaging	Het
Wfdc2	A	T	2: 164,404,706 (GRCm39)	T21S	probably benign	Het
Yod1	G	A	1: 130,645,275 (GRCm39)	G19S	probably damaging	Het
Zfp78	A	G	7: 6,381,277 (GRCm39)	E109G	probably damaging	Het
Zfp873	C	A	10: 81,894,256 (GRCm39)	A19D	probably damaging	Het
Zfpm2	G	A	15: 40,965,114 (GRCm39)	S401N	probably benign	Het

Other mutations in Zfp935

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00791:Zfp935	APN	13	62,602,278 (GRCm39)	missense	probably benign	0.33
IGL02169:Zfp935	APN	13	62,604,745 (GRCm39)	critical splice donor site	probably null
IGL02570:Zfp935	APN	13	62,604,792 (GRCm39)	missense	probably damaging	1.00
IGL02756:Zfp935	APN	13	62,602,701 (GRCm39)	nonsense	probably null
IGL03338:Zfp935	APN	13	62,602,247 (GRCm39)	missense	probably benign	0.11
R1252:Zfp935	UTSW	13	62,602,355 (GRCm39)	missense	probably damaging	1.00
R1503:Zfp935	UTSW	13	62,602,951 (GRCm39)	missense	possibly damaging	0.65
R2425:Zfp935	UTSW	13	62,602,922 (GRCm39)	missense	probably benign	0.02
R3720:Zfp935	UTSW	13	62,602,846 (GRCm39)	nonsense	probably null
R4819:Zfp935	UTSW	13	62,602,231 (GRCm39)	missense	probably damaging	1.00
R5391:Zfp935	UTSW	13	62,602,632 (GRCm39)	nonsense	probably null
R6253:Zfp935	UTSW	13	62,602,685 (GRCm39)	missense	probably benign	0.02
R9447:Zfp935	UTSW	13	62,602,842 (GRCm39)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- CTCCCGTATGTGTTCTTCTATGAA -3'
(R):5'- AACACATACTGGAGAGAAGCCTTA -3'

Sequencing Primer
(F):5'- AACTGTGTTGTGAAAAGGCTGTCAC -3'
(R):5'- CATACTGGAGAGAAGCCTTACAAATG -3'

Posted On

2018-08-01