Mutagenetix > Incidental Mutation

Incidental Mutation 'R6745:Ighg3'

530544

Institutional Source

Beutler Lab

Gene Symbol

Ighg3

Ensembl Gene

ENSMUSG00000076615

Gene Name

Immunoglobulin heavy constant gamma 3

Synonyms

IgG3, AI324046

MMRRC Submission

044862-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R6745 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

113321062-113324852 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 113323890 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 166 (V166A)

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000103423
AA Change: V166A

SMART Domains

Protein: ENSMUSP00000100219
Gene: ENSMUSG00000076615
AA Change: V166A

Domain	Start	End	E-Value	Type
IGc1	22	92	3.42e-20	SMART
low complexity region	102	114	N/A	INTRINSIC
IG_like	139	214	5.06e-3	SMART
IGc1	245	318	2.9e-32	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000223179
AA Change: V166A

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.5%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgb	G	T	10: 10,265,941 (GRCm39)	T1040K	probably damaging	Het
Adgrf3	A	G	5: 30,408,601 (GRCm39)	V59A	probably benign	Het
Ankk1	G	T	9: 49,327,480 (GRCm39)	H566Q	probably benign	Het
Avil	A	C	10: 126,849,988 (GRCm39)	D613A	probably benign	Het
Bbs9	A	G	9: 22,582,132 (GRCm39)	N613S	probably benign	Het
Chd1	A	G	17: 17,607,429 (GRCm39)	T326A	probably benign	Het
Clasp2	A	T	9: 113,704,338 (GRCm39)	R558*	probably null	Het
Cmbl	A	G	15: 31,589,933 (GRCm39)	D221G	possibly damaging	Het
Col3a1	G	T	1: 45,377,782 (GRCm39)		probably benign	Het
Creb5	A	T	6: 53,581,517 (GRCm39)	M172L	probably benign	Het
Crybg3	A	G	16: 59,372,607 (GRCm39)	V2291A	possibly damaging	Het
Cyp2g1	T	G	7: 26,513,604 (GRCm39)	V181G	probably damaging	Het
Dclk1	T	A	3: 55,385,229 (GRCm39)	S40T	probably damaging	Het
Dnah10	G	T	5: 124,885,876 (GRCm39)	M3053I	probably damaging	Het
Dnah12	T	A	14: 26,428,383 (GRCm39)	I268K	probably damaging	Het
Dock2	C	G	11: 34,596,669 (GRCm39)	D396H	probably damaging	Het
Dock2	T	A	11: 34,596,670 (GRCm39)	K395N	probably damaging	Het
Gpbp1	G	A	13: 111,589,919 (GRCm39)	R59C	probably benign	Het
Ifi47	T	G	11: 48,986,329 (GRCm39)	I32S	probably benign	Het
Katnip	T	C	7: 125,369,822 (GRCm39)	S137P	probably benign	Het
Kdm5d	T	A	Y: 927,112 (GRCm39)	C617S	probably benign	Homo
Kif20b	T	G	19: 34,906,276 (GRCm39)	S55A	possibly damaging	Het
Klhl1	C	T	14: 96,517,438 (GRCm39)		probably null	Het
Kndc1	C	T	7: 139,500,892 (GRCm39)	T727I	probably benign	Het
Lrrk1	A	T	7: 65,922,749 (GRCm39)	I298N	probably damaging	Het
Ly75	C	A	2: 60,138,523 (GRCm39)	R1448L	probably damaging	Het
Mup4	C	T	4: 59,960,091 (GRCm39)	V58M	possibly damaging	Het
Nr2c1	T	C	10: 94,026,526 (GRCm39)	F467S	probably damaging	Het
Or14j9	T	A	17: 37,874,470 (GRCm39)	H244L	probably damaging	Het
Pla2g4a	G	T	1: 149,761,981 (GRCm39)	Q151K	probably benign	Het
Pom121l2	A	T	13: 22,167,868 (GRCm39)	Q713L	probably benign	Het
Pomgnt1	T	A	4: 116,011,080 (GRCm39)	S210T	probably damaging	Het
Prb1a	T	C	6: 132,186,383 (GRCm39)		probably null	Het
Pyroxd2	T	C	19: 42,735,799 (GRCm39)	D101G	probably damaging	Het
Ranbp3	A	G	17: 57,016,308 (GRCm39)	D359G	probably benign	Het
Rgsl1	T	A	1: 153,698,063 (GRCm39)	I531F	probably benign	Het
Serpina1b	T	C	12: 103,696,614 (GRCm39)	N265S	possibly damaging	Het
Slc51b	T	C	9: 65,320,212 (GRCm39)	E85G	possibly damaging	Het
Stk32c	T	A	7: 138,702,809 (GRCm39)	R195W	probably damaging	Het
Uggt2	T	C	14: 119,280,022 (GRCm39)	T819A	possibly damaging	Het
Ugt1a9	A	T	1: 87,998,898 (GRCm39)	E116V	probably benign	Het
Zswim5	C	G	4: 116,832,401 (GRCm39)	P543A	probably damaging	Het

Other mutations in Ighg3

Allele	Source	Chr	Coord	Type	Predicted Effect
IGL00230:Ighg3	APN	12	113,323,457 (GRCm39)	missense	unknown
IGL02641:Ighg3	APN	12	113,323,818 (GRCm39)	missense	unknown
IGL03252:Ighg3	APN	12	113,324,184 (GRCm39)	missense	unknown
R0630:Ighg3	UTSW	12	113,323,714 (GRCm39)	splice site	probably benign
R4957:Ighg3	UTSW	12	113,324,750 (GRCm39)	missense	unknown
R6407:Ighg3	UTSW	12	113,323,770 (GRCm39)	missense	unknown
R7801:Ighg3	UTSW	12	113,323,436 (GRCm39)	missense
R7824:Ighg3	UTSW	12	113,323,426 (GRCm39)	missense
R8075:Ighg3	UTSW	12	113,321,097 (GRCm39)	missense
R8076:Ighg3	UTSW	12	113,324,158 (GRCm39)	missense	probably benign
R8887:Ighg3	UTSW	12	113,323,845 (GRCm39)	missense
R9314:Ighg3	UTSW	12	113,323,946 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- AGCATACACAGAGGTCGTGG -3'
(R):5'- TGGTACAGTTGCTAACCCATCC -3'

Sequencing Primer
(F):5'- AGTCAGGCTGCAGCTCTCAC -3'
(R):5'- GTACAGTTGCTAACCCATCCTATCTC -3'

Posted On

2018-08-01