Mutagenetix > Incidental Mutation

Incidental Mutation 'R6747:Cenpf'

530563

Institutional Source

Beutler Lab

Gene Symbol

Cenpf

Ensembl Gene

ENSMUSG00000026605

Gene Name

centromere protein F

Synonyms

mitosin, 6530404A22Rik, Lek1

MMRRC Submission

044864-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.433) question?

Stock #

R6747 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

189640606-189688086 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 189652854 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 2410 (T2410S)

Ref Sequence

ENSEMBL: ENSMUSP00000129738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000171929]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000165798

SMART Domains

Protein: ENSMUSP00000132430
Gene: ENSMUSG00000026605

Domain	Start	End	E-Value	Type
Pfam:CENP-F_C_Rb_bdg	23	50	1.6e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171929
AA Change: T2410S

PolyPhen 2 Score 0.153 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000129738
Gene: ENSMUSG00000026605
AA Change: T2410S

Domain	Start	End	E-Value	Type
Pfam:CENP-F_N	1	300	2.8e-144	PFAM
low complexity region	349	364	N/A	INTRINSIC
low complexity region	378	396	N/A	INTRINSIC
low complexity region	544	557	N/A	INTRINSIC
low complexity region	589	609	N/A	INTRINSIC
internal_repeat_5	678	707	8.32e-5	PROSPERO
internal_repeat_3	743	798	2.21e-5	PROSPERO
internal_repeat_1	780	812	2.12e-7	PROSPERO
coiled coil region	829	881	N/A	INTRINSIC
coiled coil region	902	1169	N/A	INTRINSIC
coiled coil region	1206	1364	N/A	INTRINSIC
internal_repeat_2	1381	1413	3.02e-6	PROSPERO
internal_repeat_3	1412	1470	2.21e-5	PROSPERO
low complexity region	1526	1542	N/A	INTRINSIC
coiled coil region	1560	1650	N/A	INTRINSIC
internal_repeat_2	1655	1687	3.02e-6	PROSPERO
low complexity region	1744	1755	N/A	INTRINSIC
coiled coil region	1822	1852	N/A	INTRINSIC
Pfam:CENP-F_leu_zip	1893	2035	1.2e-14	PFAM
Pfam:CENP-F_leu_zip	2131	2270	1.5e-47	PFAM
Pfam:CENP-F_leu_zip	2313	2449	1e-46	PFAM
low complexity region	2544	2555	N/A	INTRINSIC
low complexity region	2642	2654	N/A	INTRINSIC
low complexity region	2755	2769	N/A	INTRINSIC
internal_repeat_4	2778	2801	4.28e-5	PROSPERO
low complexity region	2837	2847	N/A	INTRINSIC
Pfam:CENP-F_C_Rb_bdg	2850	2896	6.6e-29	PFAM
internal_repeat_1	2935	2964	2.12e-7	PROSPERO
internal_repeat_4	2946	2969	4.28e-5	PROSPERO

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.0%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	C	T	3: 122,126,313 (GRCm38)		probably null	Het
Acyp1	C	T	12: 85,278,905 (GRCm38)	V107I	probably null	Het
Aftph	T	C	11: 20,726,144 (GRCm38)		probably null	Het
Agtpbp1	A	T	13: 59,544,353 (GRCm38)		probably null	Het
Arhgap15	C	A	2: 44,116,677 (GRCm38)	P269T	probably damaging	Het
Arhgap30	T	C	1: 171,407,729 (GRCm38)	V557A	probably damaging	Het
Asb3	T	A	11: 31,081,493 (GRCm38)	M410K	probably benign	Het
B4galnt2	T	C	11: 95,868,634 (GRCm38)		probably null	Het
Birc3	A	G	9: 7,860,261 (GRCm38)		probably null	Het
Cav2	A	T	6: 17,286,951 (GRCm38)	N69Y	probably damaging	Het
Cc2d2b	G	A	19: 40,795,667 (GRCm38)	C826Y	probably benign	Het
Chchd1	A	G	14: 20,703,380 (GRCm38)	D24G	probably benign	Het
Cmip	G	A	8: 117,436,879 (GRCm38)	G450S	probably benign	Het
Col11a1	C	T	3: 114,212,450 (GRCm38)	Q534*	probably null	Het
Col3a1	G	T	1: 45,338,622 (GRCm38)		probably benign	Het
Cox4i1	T	C	8: 120,673,230 (GRCm38)	I31T	possibly damaging	Het
Cstf3	G	T	2: 104,646,767 (GRCm38)	V168F	probably damaging	Het
Dapk1	T	C	13: 60,725,340 (GRCm38)	I352T	probably benign	Het
Ddx52	T	C	11: 83,955,302 (GRCm38)	V456A	probably damaging	Het
Dmxl2	A	T	9: 54,416,088 (GRCm38)	H1337Q	probably damaging	Het
Dnah7a	T	C	1: 53,636,062 (GRCm38)	T369A	probably benign	Het
Dppa1	T	A	11: 46,625,595 (GRCm38)	I8F	unknown	Het
Ebf1	G	T	11: 44,883,814 (GRCm38)	V213F	probably damaging	Het
Foxb2	C	A	19: 16,872,833 (GRCm38)	E270*	probably null	Het
Gclc	A	T	9: 77,788,245 (GRCm38)	R450W	probably damaging	Het
Gm13128	T	C	4: 144,332,978 (GRCm38)	W420R	probably benign	Het
Grin2d	T	C	7: 45,862,268 (GRCm38)	E251G	probably damaging	Het
Hal	A	T	10: 93,500,677 (GRCm38)	N423Y	probably damaging	Het
Krtap4-8	T	A	11: 99,780,091 (GRCm38)	I185F	unknown	Het
Lrp3	T	A	7: 35,211,437 (GRCm38)	Q61L	probably benign	Het
Met	A	C	6: 17,571,467 (GRCm38)	Q1296H	probably damaging	Het
Mphosph9	T	A	5: 124,297,699 (GRCm38)	N557I	possibly damaging	Het
Mrpl46	C	A	7: 78,782,981 (GRCm38)	W16C	probably benign	Het
Myh13	G	A	11: 67,350,419 (GRCm38)	R874Q	probably damaging	Het
Nelfb	G	A	2: 25,203,381 (GRCm38)	T453I	probably benign	Het
Nos2	A	G	11: 78,952,954 (GRCm38)	D909G	probably damaging	Het
Nr5a2	T	C	1: 136,882,344 (GRCm38)	E431G	possibly damaging	Het
Nsmce2	A	G	15: 59,591,724 (GRCm38)	D149G	probably benign	Het
Olfr203	T	C	16: 59,303,641 (GRCm38)	F164L	probably benign	Het
Olfr690	C	T	7: 105,330,027 (GRCm38)	R55H	probably benign	Het
Pcbp4	C	A	9: 106,460,648 (GRCm38)		probably null	Het
Peg10	A	G	6: 4,757,137 (GRCm38)		probably benign	Het
Pms2	C	T	5: 143,925,419 (GRCm38)	P154L	probably benign	Het
Pou6f2	A	C	13: 18,129,187 (GRCm38)	L112R	probably benign	Het
Prdm6	A	T	18: 53,465,046 (GRCm38)		probably benign	Het
Prob1	G	A	18: 35,655,154 (GRCm38)	R12W	probably damaging	Het
Rif1	T	A	2: 52,078,263 (GRCm38)		probably null	Het
Rpl9-ps6	A	G	19: 32,466,143 (GRCm38)	S137P	probably benign	Het
Rsf1	GGCG	GGCGACGGCAGCG	7: 97,579,906 (GRCm38)		probably benign	Homo
Sec23ip	A	G	7: 128,752,849 (GRCm38)		silent	Het
Slc38a9	T	C	13: 112,690,180 (GRCm38)	C151R	probably benign	Het
Slc39a8	T	C	3: 135,849,180 (GRCm38)		probably null	Het
Slc6a18	C	A	13: 73,677,991 (GRCm38)		probably benign	Het
Snw1	T	C	12: 87,464,710 (GRCm38)	D57G	probably damaging	Het
Sox6	C	G	7: 115,541,731 (GRCm38)	R505P	probably damaging	Het
Speg	T	C	1: 75,410,395 (GRCm38)		probably null	Het
Spire2	C	T	8: 123,356,846 (GRCm38)	R190C	probably damaging	Het
Stard9	A	C	2: 120,698,383 (GRCm38)	H1707P	possibly damaging	Het
Tenm3	G	T	8: 48,343,243 (GRCm38)	T237K	probably damaging	Het
Themis2	T	A	4: 132,796,262 (GRCm38)	E31V	possibly damaging	Het
Trim56	T	G	5: 137,114,521 (GRCm38)	Q47P	probably damaging	Het
Trim58	T	C	11: 58,651,264 (GRCm38)	I350T	probably benign	Het
Ttll7	C	T	3: 146,944,056 (GRCm38)	P639S	probably benign	Het
Ubxn6	A	G	17: 56,070,650 (GRCm38)		probably null	Het
Vmn2r114	A	T	17: 23,309,876 (GRCm38)	F417L	probably benign	Het
Vmn2r29	T	A	7: 7,231,422 (GRCm38)	M822L	probably benign	Het
Vps9d1	T	C	8: 123,254,007 (GRCm38)	D27G	probably damaging	Het
Wdr3	C	T	3: 100,138,724 (GRCm38)	R931Q	probably damaging	Het
Whamm	G	A	7: 81,578,302 (GRCm38)		probably null	Het
Zcchc24	T	C	14: 25,757,033 (GRCm38)	H142R	probably damaging	Het
Zfp292	T	C	4: 34,806,894 (GRCm38)	K2050R	probably damaging	Het

Other mutations in Cenpf

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00778:Cenpf	APN	1	189,654,912 (GRCm38)	missense	probably benign	0.01
IGL01154:Cenpf	APN	1	189,680,333 (GRCm38)	missense	probably benign	0.19
IGL01434:Cenpf	APN	1	189,657,868 (GRCm38)	nonsense	probably null
IGL01461:Cenpf	APN	1	189,657,096 (GRCm38)	missense	probably damaging	1.00
IGL01615:Cenpf	APN	1	189,653,184 (GRCm38)	missense	possibly damaging	0.68
IGL01720:Cenpf	APN	1	189,651,215 (GRCm38)	missense	probably damaging	0.99
IGL01720:Cenpf	APN	1	189,682,386 (GRCm38)	missense	probably benign	0.05
IGL01803:Cenpf	APN	1	189,654,771 (GRCm38)	nonsense	probably null
IGL02152:Cenpf	APN	1	189,649,012 (GRCm38)	missense	probably benign
IGL02222:Cenpf	APN	1	189,654,444 (GRCm38)	missense	probably benign
IGL02338:Cenpf	APN	1	189,680,418 (GRCm38)	missense	probably damaging	1.00
IGL02580:Cenpf	APN	1	189,657,441 (GRCm38)	missense	probably benign	0.20
IGL02629:Cenpf	APN	1	189,652,334 (GRCm38)	missense	probably damaging	1.00
IGL02650:Cenpf	APN	1	189,652,473 (GRCm38)	missense	possibly damaging	0.91
IGL02660:Cenpf	APN	1	189,654,782 (GRCm38)	missense	probably damaging	1.00
IGL02703:Cenpf	APN	1	189,659,758 (GRCm38)	missense	probably benign	0.14
IGL02809:Cenpf	APN	1	189,682,358 (GRCm38)	splice site	probably benign
IGL02851:Cenpf	APN	1	189,658,030 (GRCm38)	missense	probably damaging	1.00
IGL02903:Cenpf	APN	1	189,646,876 (GRCm38)	missense	probably damaging	0.99
IGL03126:Cenpf	APN	1	189,659,010 (GRCm38)	missense	probably damaging	1.00
IGL03235:Cenpf	APN	1	189,683,927 (GRCm38)	missense	probably damaging	1.00
IGL03336:Cenpf	APN	1	189,652,647 (GRCm38)	missense	probably damaging	0.99
IGL03402:Cenpf	APN	1	189,655,076 (GRCm38)	missense	probably damaging	1.00
IGL02799:Cenpf	UTSW	1	189,659,652 (GRCm38)	missense	probably damaging	1.00
R0011:Cenpf	UTSW	1	189,650,706 (GRCm38)	missense	probably benign	0.05
R0129:Cenpf	UTSW	1	189,659,650 (GRCm38)	missense	probably benign	0.26
R0157:Cenpf	UTSW	1	189,652,359 (GRCm38)	missense	probably benign	0.07
R0270:Cenpf	UTSW	1	189,650,714 (GRCm38)	missense	probably benign	0.01
R0607:Cenpf	UTSW	1	189,682,463 (GRCm38)	splice site	probably null
R0621:Cenpf	UTSW	1	189,672,628 (GRCm38)	missense	probably benign
R0639:Cenpf	UTSW	1	189,658,062 (GRCm38)	missense	probably benign	0.01
R0653:Cenpf	UTSW	1	189,659,986 (GRCm38)	missense	probably damaging	1.00
R0718:Cenpf	UTSW	1	189,653,984 (GRCm38)	missense	probably damaging	1.00
R1157:Cenpf	UTSW	1	189,658,453 (GRCm38)	missense	probably benign	0.20
R1331:Cenpf	UTSW	1	189,642,801 (GRCm38)	missense	probably damaging	0.99
R1463:Cenpf	UTSW	1	189,654,739 (GRCm38)	missense	probably damaging	0.97
R1514:Cenpf	UTSW	1	189,679,141 (GRCm38)	missense	possibly damaging	0.67
R1529:Cenpf	UTSW	1	189,660,038 (GRCm38)	missense	probably benign	0.00
R1574:Cenpf	UTSW	1	189,652,713 (GRCm38)	missense	probably damaging	1.00
R1574:Cenpf	UTSW	1	189,652,713 (GRCm38)	missense	probably damaging	1.00
R1662:Cenpf	UTSW	1	189,657,771 (GRCm38)	missense	probably damaging	0.99
R1671:Cenpf	UTSW	1	189,679,144 (GRCm38)	splice site	probably null
R1725:Cenpf	UTSW	1	189,680,479 (GRCm38)	missense	probably damaging	0.99
R1743:Cenpf	UTSW	1	189,654,263 (GRCm38)	missense	probably benign	0.19
R1874:Cenpf	UTSW	1	189,683,816 (GRCm38)	missense	probably damaging	1.00
R1884:Cenpf	UTSW	1	189,646,849 (GRCm38)	missense	probably benign
R1980:Cenpf	UTSW	1	189,653,915 (GRCm38)	missense	probably benign	0.04
R2074:Cenpf	UTSW	1	189,656,901 (GRCm38)	missense	probably damaging	1.00
R2096:Cenpf	UTSW	1	189,653,459 (GRCm38)	missense	possibly damaging	0.95
R2109:Cenpf	UTSW	1	189,679,067 (GRCm38)	missense	probably damaging	0.99
R2113:Cenpf	UTSW	1	189,679,102 (GRCm38)	missense	probably damaging	0.96
R2134:Cenpf	UTSW	1	189,658,642 (GRCm38)	missense	probably benign	0.03
R2209:Cenpf	UTSW	1	189,652,598 (GRCm38)	missense	probably benign	0.04
R2875:Cenpf	UTSW	1	189,658,644 (GRCm38)	missense	probably benign	0.11
R2876:Cenpf	UTSW	1	189,658,644 (GRCm38)	missense	probably benign	0.11
R3433:Cenpf	UTSW	1	189,659,949 (GRCm38)	missense	probably damaging	0.99
R3709:Cenpf	UTSW	1	189,648,812 (GRCm38)	missense	possibly damaging	0.72
R3786:Cenpf	UTSW	1	189,658,337 (GRCm38)	missense	probably damaging	1.00
R4014:Cenpf	UTSW	1	189,653,159 (GRCm38)	missense	probably benign	0.01
R4108:Cenpf	UTSW	1	189,683,868 (GRCm38)	missense	probably damaging	1.00
R4119:Cenpf	UTSW	1	189,653,045 (GRCm38)	missense	probably benign	0.01
R4177:Cenpf	UTSW	1	189,668,619 (GRCm38)	missense	possibly damaging	0.95
R4422:Cenpf	UTSW	1	189,658,350 (GRCm38)	missense	probably damaging	1.00
R4546:Cenpf	UTSW	1	189,654,650 (GRCm38)	missense	probably damaging	1.00
R4592:Cenpf	UTSW	1	189,679,033 (GRCm38)	missense	probably damaging	1.00
R4643:Cenpf	UTSW	1	189,659,589 (GRCm38)	missense	probably benign	0.00
R4650:Cenpf	UTSW	1	189,660,038 (GRCm38)	missense	probably benign	0.00
R4801:Cenpf	UTSW	1	189,651,220 (GRCm38)	missense	probably damaging	1.00
R4802:Cenpf	UTSW	1	189,651,220 (GRCm38)	missense	probably damaging	1.00
R4817:Cenpf	UTSW	1	189,682,369 (GRCm38)	missense	possibly damaging	0.93
R4871:Cenpf	UTSW	1	189,658,531 (GRCm38)	missense	probably damaging	1.00
R5037:Cenpf	UTSW	1	189,683,846 (GRCm38)	missense	probably damaging	1.00
R5106:Cenpf	UTSW	1	189,683,808 (GRCm38)	missense	probably benign	0.00
R5208:Cenpf	UTSW	1	189,671,046 (GRCm38)	critical splice donor site	probably null
R5213:Cenpf	UTSW	1	189,654,980 (GRCm38)	missense	probably benign	0.04
R5237:Cenpf	UTSW	1	189,659,533 (GRCm38)	missense	probably benign	0.28
R5255:Cenpf	UTSW	1	189,672,627 (GRCm38)	missense	possibly damaging	0.49
R5378:Cenpf	UTSW	1	189,653,466 (GRCm38)	missense	possibly damaging	0.95
R5468:Cenpf	UTSW	1	189,652,371 (GRCm38)	missense	probably damaging	1.00
R5510:Cenpf	UTSW	1	189,682,903 (GRCm38)	missense	probably benign	0.14
R5616:Cenpf	UTSW	1	189,657,286 (GRCm38)	missense	probably damaging	1.00
R5652:Cenpf	UTSW	1	189,657,082 (GRCm38)	missense	probably damaging	0.99
R5735:Cenpf	UTSW	1	189,654,363 (GRCm38)	missense	probably benign	0.10
R5841:Cenpf	UTSW	1	189,657,444 (GRCm38)	missense	possibly damaging	0.90
R5943:Cenpf	UTSW	1	189,659,969 (GRCm38)	missense	possibly damaging	0.75
R6082:Cenpf	UTSW	1	189,658,104 (GRCm38)	missense	probably benign	0.11
R6108:Cenpf	UTSW	1	189,662,013 (GRCm38)	missense	probably benign	0.03
R6269:Cenpf	UTSW	1	189,659,920 (GRCm38)	missense	probably benign	0.37
R6284:Cenpf	UTSW	1	189,652,742 (GRCm38)	missense	probably damaging	1.00
R6425:Cenpf	UTSW	1	189,659,898 (GRCm38)	missense	probably benign	0.09
R6587:Cenpf	UTSW	1	189,658,374 (GRCm38)	missense	probably damaging	1.00
R6811:Cenpf	UTSW	1	189,654,542 (GRCm38)	missense	probably benign	0.06
R6834:Cenpf	UTSW	1	189,659,446 (GRCm38)	missense	probably damaging	1.00
R6951:Cenpf	UTSW	1	189,653,792 (GRCm38)	missense	probably damaging	1.00
R7095:Cenpf	UTSW	1	189,659,176 (GRCm38)	missense	probably benign	0.01
R7128:Cenpf	UTSW	1	189,684,991 (GRCm38)	missense	probably damaging	1.00
R7185:Cenpf	UTSW	1	189,653,489 (GRCm38)	missense	probably damaging	1.00
R7292:Cenpf	UTSW	1	189,650,694 (GRCm38)	missense	probably damaging	1.00
R7353:Cenpf	UTSW	1	189,654,138 (GRCm38)	nonsense	probably null
R7402:Cenpf	UTSW	1	189,659,378 (GRCm38)	nonsense	probably null
R7460:Cenpf	UTSW	1	189,654,050 (GRCm38)	missense	probably damaging	0.97
R7484:Cenpf	UTSW	1	189,656,821 (GRCm38)	missense	probably damaging	1.00
R7574:Cenpf	UTSW	1	189,658,667 (GRCm38)	missense	probably damaging	1.00
R7691:Cenpf	UTSW	1	189,658,207 (GRCm38)	nonsense	probably null
R7698:Cenpf	UTSW	1	189,662,072 (GRCm38)	missense	probably benign	0.01
R7901:Cenpf	UTSW	1	189,657,248 (GRCm38)	missense	probably damaging	1.00
R7941:Cenpf	UTSW	1	189,657,286 (GRCm38)	missense	probably damaging	1.00
R8007:Cenpf	UTSW	1	189,646,947 (GRCm38)	missense
R8194:Cenpf	UTSW	1	189,682,403 (GRCm38)	missense	probably benign	0.06
R8420:Cenpf	UTSW	1	189,672,585 (GRCm38)	missense	probably damaging	1.00
R8429:Cenpf	UTSW	1	189,657,307 (GRCm38)	missense	possibly damaging	0.72
R8477:Cenpf	UTSW	1	189,653,188 (GRCm38)	missense	probably benign
R8492:Cenpf	UTSW	1	189,658,729 (GRCm38)	missense	probably damaging	0.99
R8510:Cenpf	UTSW	1	189,650,706 (GRCm38)	missense	probably benign	0.01
R8686:Cenpf	UTSW	1	189,659,604 (GRCm38)	missense	probably benign	0.00
R8696:Cenpf	UTSW	1	189,657,997 (GRCm38)	missense	probably benign	0.20
R8855:Cenpf	UTSW	1	189,653,233 (GRCm38)	missense	probably benign	0.11
R8901:Cenpf	UTSW	1	189,662,051 (GRCm38)	missense	probably benign	0.30
R8958:Cenpf	UTSW	1	189,653,153 (GRCm38)	missense	possibly damaging	0.81
R9109:Cenpf	UTSW	1	189,659,374 (GRCm38)	missense	probably benign	0.06
R9135:Cenpf	UTSW	1	189,672,549 (GRCm38)	missense	probably damaging	1.00
R9136:Cenpf	UTSW	1	189,671,155 (GRCm38)	missense	probably benign	0.02
R9198:Cenpf	UTSW	1	189,656,790 (GRCm38)	missense	probably damaging	1.00
R9240:Cenpf	UTSW	1	189,656,970 (GRCm38)	missense	probably benign	0.01
R9303:Cenpf	UTSW	1	189,660,074 (GRCm38)	critical splice acceptor site	probably null
R9305:Cenpf	UTSW	1	189,660,074 (GRCm38)	critical splice acceptor site	probably null
R9354:Cenpf	UTSW	1	189,646,917 (GRCm38)	missense
R9502:Cenpf	UTSW	1	189,656,781 (GRCm38)	missense	probably damaging	1.00
R9619:Cenpf	UTSW	1	189,653,768 (GRCm38)	missense	probably benign	0.01
RF006:Cenpf	UTSW	1	189,657,386 (GRCm38)	missense	probably damaging	1.00
X0025:Cenpf	UTSW	1	189,653,874 (GRCm38)	missense	possibly damaging	0.79
X0066:Cenpf	UTSW	1	189,657,929 (GRCm38)	missense	probably benign	0.23
Z1088:Cenpf	UTSW	1	189,652,931 (GRCm38)	missense	probably damaging	1.00
Z1176:Cenpf	UTSW	1	189,659,472 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GACTACCCAGATTCTGTTCTTTGG -3'
(R):5'- ACCGAGTTGAGAACCTTGAAC -3'

Sequencing Primer
(F):5'- CCAGATTCTGTTCTTTGGTCTTAGAG -3'
(R):5'- AGTTGAGAACCTTGAACAAGAATTG -3'

Posted On

2018-08-01