Mutagenetix > Incidental Mutation

Incidental Mutation 'R6747:Olfr203'

530616

Institutional Source

Beutler Lab

Gene Symbol

Olfr203

Ensembl Gene

ENSMUSG00000068182

Gene Name

olfactory receptor 203

Synonyms

MOR182-5, GA_x54KRFPKG5P-55517445-55518365

MMRRC Submission

044864-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R6747 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

59298881-59304413 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 59303641 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 164 (F164L)

Ref Sequence

ENSEMBL: ENSMUSP00000086719 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089305] [ENSMUST00000215893]

AlphaFold

L7N205

Predicted Effect

probably benign
Transcript: ENSMUST00000089305
AA Change: F164L

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000086719
Gene: ENSMUSG00000068182
AA Change: F164L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1e-46	PFAM
Pfam:7tm_1	41	290	7.6e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215893
AA Change: F163L

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.0%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	C	T	3: 122,126,313		probably null	Het
Acyp1	C	T	12: 85,278,905	V107I	probably null	Het
Aftph	T	C	11: 20,726,144		probably null	Het
Agtpbp1	A	T	13: 59,544,353		probably null	Het
Arhgap15	C	A	2: 44,116,677	P269T	probably damaging	Het
Arhgap30	T	C	1: 171,407,729	V557A	probably damaging	Het
Asb3	T	A	11: 31,081,493	M410K	probably benign	Het
B4galnt2	T	C	11: 95,868,634		probably null	Het
Birc3	A	G	9: 7,860,261		probably null	Het
Cav2	A	T	6: 17,286,951	N69Y	probably damaging	Het
Cc2d2b	G	A	19: 40,795,667	C826Y	probably benign	Het
Cenpf	T	A	1: 189,652,854	T2410S	probably benign	Het
Chchd1	A	G	14: 20,703,380	D24G	probably benign	Het
Cmip	G	A	8: 117,436,879	G450S	probably benign	Het
Col11a1	C	T	3: 114,212,450	Q534*	probably null	Het
Col3a1	G	T	1: 45,338,622		probably benign	Het
Cox4i1	T	C	8: 120,673,230	I31T	possibly damaging	Het
Cstf3	G	T	2: 104,646,767	V168F	probably damaging	Het
Dapk1	T	C	13: 60,725,340	I352T	probably benign	Het
Ddx52	T	C	11: 83,955,302	V456A	probably damaging	Het
Dmxl2	A	T	9: 54,416,088	H1337Q	probably damaging	Het
Dnah7a	T	C	1: 53,636,062	T369A	probably benign	Het
Dppa1	T	A	11: 46,625,595	I8F	unknown	Het
Ebf1	G	T	11: 44,883,814	V213F	probably damaging	Het
Foxb2	C	A	19: 16,872,833	E270*	probably null	Het
Gclc	A	T	9: 77,788,245	R450W	probably damaging	Het
Gm13128	T	C	4: 144,332,978	W420R	probably benign	Het
Grin2d	T	C	7: 45,862,268	E251G	probably damaging	Het
Hal	A	T	10: 93,500,677	N423Y	probably damaging	Het
Krtap4-8	T	A	11: 99,780,091	I185F	unknown	Het
Lrp3	T	A	7: 35,211,437	Q61L	probably benign	Het
Met	A	C	6: 17,571,467	Q1296H	probably damaging	Het
Mphosph9	T	A	5: 124,297,699	N557I	possibly damaging	Het
Mrpl46	C	A	7: 78,782,981	W16C	probably benign	Het
Myh13	G	A	11: 67,350,419	R874Q	probably damaging	Het
Nelfb	G	A	2: 25,203,381	T453I	probably benign	Het
Nos2	A	G	11: 78,952,954	D909G	probably damaging	Het
Nr5a2	T	C	1: 136,882,344	E431G	possibly damaging	Het
Nsmce2	A	G	15: 59,591,724	D149G	probably benign	Het
Olfr690	C	T	7: 105,330,027	R55H	probably benign	Het
Pcbp4	C	A	9: 106,460,648		probably null	Het
Peg10	A	G	6: 4,757,137		probably benign	Het
Pms2	C	T	5: 143,925,419	P154L	probably benign	Het
Pou6f2	A	C	13: 18,129,187	L112R	probably benign	Het
Prdm6	A	T	18: 53,465,046		probably benign	Het
Prob1	G	A	18: 35,655,154	R12W	probably damaging	Het
Rif1	T	A	2: 52,078,263		probably null	Het
Rpl9-ps6	A	G	19: 32,466,143	S137P	probably benign	Het
Rsf1	GGCG	GGCGACGGCAGCG	7: 97,579,906		probably benign	Homo
Sec23ip	A	G	7: 128,752,849		silent	Het
Slc38a9	T	C	13: 112,690,180	C151R	probably benign	Het
Slc39a8	T	C	3: 135,849,180		probably null	Het
Slc6a18	C	A	13: 73,677,991		probably benign	Het
Snw1	T	C	12: 87,464,710	D57G	probably damaging	Het
Sox6	C	G	7: 115,541,731	R505P	probably damaging	Het
Speg	T	C	1: 75,410,395		probably null	Het
Spire2	C	T	8: 123,356,846	R190C	probably damaging	Het
Stard9	A	C	2: 120,698,383	H1707P	possibly damaging	Het
Tenm3	G	T	8: 48,343,243	T237K	probably damaging	Het
Themis2	T	A	4: 132,796,262	E31V	possibly damaging	Het
Trim56	T	G	5: 137,114,521	Q47P	probably damaging	Het
Trim58	T	C	11: 58,651,264	I350T	probably benign	Het
Ttll7	C	T	3: 146,944,056	P639S	probably benign	Het
Ubxn6	A	G	17: 56,070,650		probably null	Het
Vmn2r114	A	T	17: 23,309,876	F417L	probably benign	Het
Vmn2r29	T	A	7: 7,231,422	M822L	probably benign	Het
Vps9d1	T	C	8: 123,254,007	D27G	probably damaging	Het
Wdr3	C	T	3: 100,138,724	R931Q	probably damaging	Het
Whamm	G	A	7: 81,578,302		probably null	Het
Zcchc24	T	C	14: 25,757,033	H142R	probably damaging	Het
Zfp292	T	C	4: 34,806,894	K2050R	probably damaging	Het

Other mutations in Olfr203

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02003:Olfr203	APN	16	59303633	missense	probably damaging	0.99
IGL02294:Olfr203	APN	16	59303612	missense	probably damaging	1.00
IGL02412:Olfr203	APN	16	59303192	missense	probably damaging	1.00
IGL02899:Olfr203	APN	16	59303286	missense	probably damaging	1.00
R0792:Olfr203	UTSW	16	59303989	missense	probably damaging	0.99
R1551:Olfr203	UTSW	16	59303403	missense	probably benign	0.03
R1701:Olfr203	UTSW	16	59303288	missense	probably benign	0.23
R1975:Olfr203	UTSW	16	59303728	missense	probably damaging	0.98
R2272:Olfr203	UTSW	16	59303444	missense	possibly damaging	0.55
R5199:Olfr203	UTSW	16	59303740	missense	probably benign
R5843:Olfr203	UTSW	16	59303361	missense	probably damaging	1.00
R5928:Olfr203	UTSW	16	59303158	missense	probably damaging	1.00
R6708:Olfr203	UTSW	16	59304053	missense	probably damaging	1.00
R6894:Olfr203	UTSW	16	59303779	missense	probably damaging	0.98
R7324:Olfr203	UTSW	16	59303248	missense	probably benign
R7380:Olfr203	UTSW	16	59304028	missense	probably damaging	1.00
R7612:Olfr203	UTSW	16	59303627	missense	probably damaging	1.00
R7775:Olfr203	UTSW	16	59303251	missense	probably damaging	1.00
R8010:Olfr203	UTSW	16	59303504	missense	probably damaging	1.00
R8408:Olfr203	UTSW	16	59304055	nonsense	probably null
R8424:Olfr203	UTSW	16	59303409	missense	possibly damaging	0.60
R8746:Olfr203	UTSW	16	59303610	missense	probably benign	0.03
Z1176:Olfr203	UTSW	16	59303169	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AGTGCAACTACAGAATGTTTCCTTC -3'
(R):5'- GCAGGTGGAGAAGGCTTTTC -3'

Sequencing Primer
(F):5'- ACAGAATGTTTCCTTCTGGTAGC -3'
(R):5'- TTTGTTTTCAGGATGGAAAAGAGGAC -3'

Posted On

2018-08-01