Mutagenetix > Incidental Mutation

Incidental Mutation 'R6751:Vmn1r158'

530769

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r158

Ensembl Gene

ENSMUSG00000094700

Gene Name

vomeronasal 1 receptor 158

Synonyms

Gm16455

MMRRC Submission

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.687) question?

Stock #

R6751 (G1)

Quality Score

125.008

Status

Not validated

Chromosome

Chromosomal Location

22789859-22790782 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 22789881 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 301 (C301Y)

Ref Sequence

ENSEMBL: ENSMUSP00000133990 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000174643]

AlphaFold

G3UY92

Predicted Effect

probably damaging
Transcript: ENSMUST00000174643
AA Change: C301Y

PolyPhen 2 Score 0.957 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000133990
Gene: ENSMUSG00000094700
AA Change: C301Y

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	298	2.9e-15	PFAM
Pfam:7tm_1	39	290	5.5e-9	PFAM
Pfam:V1R	41	298	3.2e-17	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.4%
20x: 95.7%

Validation Efficiency

95% (59/62)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 138,066,210	N387Y	probably damaging	Het
4932415D10Rik	A	G	10: 82,283,497	S4560P	probably benign	Het
Abhd17a	T	C	10: 80,586,587	E87G	probably damaging	Het
Aco1	T	A	4: 40,188,330		probably null	Het
Adcy6	T	C	15: 98,596,205	N817S	probably benign	Het
Ak8	T	A	2: 28,709,945	L63*	probably null	Het
Arhgef28	G	A	13: 98,075,247	S76L	probably damaging	Het
Asap1	A	G	15: 64,094,412	L891S	possibly damaging	Het
Cacng5	C	A	11: 107,877,553	M209I	probably benign	Het
Casr	T	C	16: 36,515,588	I120V	probably benign	Het
Chd7	T	C	4: 8,833,866	Y1207H	probably damaging	Het
Chrnb2	A	T	3: 89,761,576	F144Y	probably damaging	Het
Cyp4a14	T	A	4: 115,491,194	H362L	probably damaging	Het
Dym	G	A	18: 75,286,647	V630M	probably damaging	Het
Dyrk1b	C	A	7: 28,186,709	P619Q	probably damaging	Het
Dyx1c1	A	G	9: 72,961,975	T156A	probably benign	Het
Eml5	T	C	12: 98,865,400	D433G	probably damaging	Het
Fam58b	T	C	11: 78,751,124	Y180C	probably damaging	Het
Frem2	A	T	3: 53,653,665	S1140R	probably damaging	Het
Gabra5	T	C	7: 57,418,334	R255G	probably damaging	Het
Galnt17	T	A	5: 131,081,590	I304F	probably damaging	Het
Gpc5	A	G	14: 115,369,951	S322G	probably benign	Het
Herc1	TCCC	TCC	9: 66,501,188		probably null	Het
Hmcn1	T	A	1: 150,734,518	N1467Y	probably damaging	Het
Ifna6	T	C	4: 88,827,750	L112P	probably damaging	Het
Ifrd1	C	T	12: 40,203,914		probably null	Het
Il17f	A	G	1: 20,779,489	M17T	probably benign	Het
Itga11	G	A	9: 62,768,584	V892I	probably benign	Het
Nckap5l	A	G	15: 99,423,161	L1246P	probably damaging	Het
Nlrp4f	A	T	13: 65,194,429	H467Q	probably damaging	Het
Ntng2	A	G	2: 29,228,043	V131A	possibly damaging	Het
Olfr550	G	A	7: 102,578,499	M1I	probably null	Het
Olfr889	T	G	9: 38,115,975	Y60D	probably damaging	Het
Olfr970	T	A	9: 39,819,897	V86E	probably benign	Het
Olfr972	T	C	9: 39,873,680	V135A	probably benign	Het
Osbpl8	T	C	10: 111,275,013	Y459H	possibly damaging	Het
Pabpc1	A	T	15: 36,597,534	V537D	possibly damaging	Het
Pde4b	A	T	4: 102,602,671	M583L	probably damaging	Het
Phlda1	T	C	10: 111,506,694	V97A	possibly damaging	Het
Pik3cb	G	T	9: 99,094,521	H174Q	probably benign	Het
Plxdc2	A	T	2: 16,548,141	I117F	probably benign	Het
Psapl1	C	A	5: 36,204,959	C298*	probably null	Het
Rsf1	G	GACGGCGGCT	7: 97,579,909		probably benign	Homo
Rtkn2	T	A	10: 68,041,453	F448I	probably benign	Het
Scn10a	T	C	9: 119,671,551	R221G	probably damaging	Het
Serpina3c	A	G	12: 104,151,500	L193P	probably damaging	Het
Sox12	T	C	2: 152,396,758	Y314C	probably damaging	Het
Sptbn1	T	C	11: 30,117,859	E1772G	probably damaging	Het
Supt6	A	G	11: 78,208,949	V1570A	probably benign	Het
Synrg	T	C	11: 83,981,425	F125S	probably damaging	Het
Tenm4	A	T	7: 96,845,712	I1116F	possibly damaging	Het
Tfap2a	T	A	13: 40,728,754	N25I	probably damaging	Het
Tfap2d	C	A	1: 19,103,283	H10N	possibly damaging	Het
Trpm8	T	C	1: 88,384,706	I1103T	possibly damaging	Het
Vmn2r56	T	C	7: 12,694,792	I516V	probably benign	Het
Vmn2r71	G	A	7: 85,619,887		probably null	Het
Vnn3	C	T	10: 23,869,625	R491C	probably benign	Het
Vps50	A	G	6: 3,600,274	Y911C	probably damaging	Het
Wdr60	A	T	12: 116,213,456	V842E	possibly damaging	Het
Zfp772	C	T	7: 7,203,717	R325Q	possibly damaging	Het
Zranb3	T	A	1: 127,959,819	H957L	probably benign	Het
Zscan20	G	T	4: 128,585,875	T941K	probably damaging	Het
Zscan25	T	A	5: 145,290,563	F346I	probably damaging	Het

Other mutations in Vmn1r158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01147:Vmn1r158	APN	7	22790779	missense	probably benign	0.01
R1173:Vmn1r158	UTSW	7	22790445	missense	probably benign	0.19
R1574:Vmn1r158	UTSW	7	22790347	missense	probably damaging	1.00
R1574:Vmn1r158	UTSW	7	22790347	missense	probably damaging	1.00
R1725:Vmn1r158	UTSW	7	22790647	missense	probably benign	0.08
R1777:Vmn1r158	UTSW	7	22790430	missense	probably damaging	1.00
R1813:Vmn1r158	UTSW	7	22790718	missense	probably damaging	1.00
R1896:Vmn1r158	UTSW	7	22790718	missense	probably damaging	1.00
R2077:Vmn1r158	UTSW	7	22790390	missense	probably benign	0.03
R3749:Vmn1r158	UTSW	7	22790214	missense	probably damaging	1.00
R4872:Vmn1r158	UTSW	7	22790754	missense	possibly damaging	0.94
R5238:Vmn1r158	UTSW	7	22790374	missense	probably benign
R6500:Vmn1r158	UTSW	7	22790653	missense	possibly damaging	0.89
R6511:Vmn1r158	UTSW	7	22790691	missense	probably benign	0.00
R6581:Vmn1r158	UTSW	7	22790040	missense	possibly damaging	0.69
R7168:Vmn1r158	UTSW	7	22790676	missense	possibly damaging	0.82
R7337:Vmn1r158	UTSW	7	22790224	missense	probably benign	0.00
R7747:Vmn1r158	UTSW	7	22790300	missense	probably benign	0.15
R7902:Vmn1r158	UTSW	7	22790008	missense	possibly damaging	0.67
R8328:Vmn1r158	UTSW	7	22790062	missense	probably damaging	1.00
R8400:Vmn1r158	UTSW	7	22789880	nonsense	probably null
R8809:Vmn1r158	UTSW	7	22790350	missense	probably damaging	0.97
R9227:Vmn1r158	UTSW	7	22790044	missense	probably benign	0.02
R9468:Vmn1r158	UTSW	7	22790463	missense	probably damaging	1.00
Z1177:Vmn1r158	UTSW	7	22790458	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- CATCAGTTTCAGTCCAGCCTGC -3'
(R):5'- ATCCTGATGCTGGTGGTCAC -3'

Sequencing Primer
(F):5'- GCCTGCAGCAGAATACAGTTATATAC -3'
(R):5'- CCTGATGCTGGTGGTCACATTTG -3'

Posted On

2018-08-01