Mutagenetix > Incidental Mutation

Incidental Mutation 'R6753:Otog'

530892

Institutional Source

Beutler Lab

Gene Symbol

Otog

Ensembl Gene

ENSMUSG00000009487

Gene Name

otogelin

Synonyms

Otgn

MMRRC Submission

044870-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.844) question?

Stock #

R6753 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

46240987-46311434 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 46249071 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Aspartic acid at position 204 (E204D)

Ref Sequence

ENSEMBL: ENSMUSP00000130949 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164538]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000164538
AA Change: E204D

PolyPhen 2 Score 0.060 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000130949
Gene: ENSMUSG00000009487
AA Change: E204D

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
low complexity region	72	85	N/A	INTRINSIC
VWD	128	288	7.98e-45	SMART
C8	330	404	1.05e-13	SMART
VWC	463	505	1.24e0	SMART
VWD	490	655	4.94e-50	SMART
C8	693	758	1.23e-5	SMART
Pfam:TIL	767	831	3.4e-13	PFAM
VWC	935	983	1.83e0	SMART
VWD	962	1118	6.05e-45	SMART
C8	1153	1227	1.02e-34	SMART
Pfam:AbfB	1270	1384	7.5e-10	PFAM
low complexity region	1488	1513	N/A	INTRINSIC
low complexity region	1524	1536	N/A	INTRINSIC
low complexity region	1560	1578	N/A	INTRINSIC
low complexity region	1637	1644	N/A	INTRINSIC
low complexity region	1677	1696	N/A	INTRINSIC
low complexity region	1731	1748	N/A	INTRINSIC
VWD	2087	2251	2.37e-29	SMART
C8	2287	2356	4.93e-19	SMART
low complexity region	2443	2449	N/A	INTRINSIC
CT	2828	2911	3.46e-28	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210281

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.1%

Validation Efficiency

99% (68/69)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]
PHENOTYPE: Homozygotes for a number of different spontaneous and targeted mutations exhibit vestibular dysfunction, including circling, head tilt, impaired balance, coordination, and placing response. Mutants have impaired hearing, decreased brain stem auditory evoked potential, and ear abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	G	A	11: 120,010,151 (GRCm38)	P1083S	probably benign	Het
Abcb5	T	A	12: 118,944,906 (GRCm38)	N101I	possibly damaging	Het
Adrb2	A	G	18: 62,179,553 (GRCm38)	V67A	possibly damaging	Het
Agk	T	A	6: 40,368,570 (GRCm38)		probably null	Het
Akap10	A	T	11: 61,886,777 (GRCm38)	M586K	probably damaging	Het
Akt3	A	T	1: 177,050,190 (GRCm38)	Y337*	probably null	Het
Armc1	A	G	3: 19,144,398 (GRCm38)	F133L	possibly damaging	Het
Bank1	T	C	3: 136,093,308 (GRCm38)	E424G	probably damaging	Het
Cacna1a	A	G	8: 84,580,205 (GRCm38)	E1363G	probably damaging	Het
Cacna1d	C	A	14: 30,042,786 (GRCm38)	A2076S	probably damaging	Het
Ccdc73	T	A	2: 104,991,524 (GRCm38)	L606*	probably null	Het
Ccdc8	C	T	7: 16,996,637 (GRCm38)	Q684*	probably null	Het
Ces1b	T	A	8: 93,067,020 (GRCm38)	K314*	probably null	Het
Ces1e	T	A	8: 93,215,128 (GRCm38)	N238I	probably damaging	Het
Chd4	A	G	6: 125,114,300 (GRCm38)	N1238S	probably benign	Het
Cmtr2	T	A	8: 110,222,979 (GRCm38)	D640E	probably damaging	Het
Col7a1	C	T	9: 108,958,128 (GRCm38)	T559I	unknown	Het
Comt	T	C	16: 18,408,021 (GRCm38)	K205R	probably benign	Het
Dbp	A	T	7: 45,708,404 (GRCm38)	E232V	probably damaging	Het
Dcbld2	A	G	16: 58,456,130 (GRCm38)	T470A	possibly damaging	Het
Eml6	T	A	11: 29,754,987 (GRCm38)	D1519V	probably damaging	Het
Evpl	T	A	11: 116,237,906 (GRCm38)	H31L	possibly damaging	Het
Exoc1	T	A	5: 76,563,339 (GRCm38)	I86N	probably damaging	Het
Fat3	C	T	9: 15,915,061 (GRCm38)	E4532K	possibly damaging	Het
Fgfr3	T	C	5: 33,732,159 (GRCm38)	S301P	probably benign	Het
Gas2l1	C	T	11: 5,064,254 (GRCm38)	V69I	probably damaging	Het
Gm2042	T	A	12: 87,958,084 (GRCm38)	I107K	probably damaging	Het
Gucy1b1	T	C	3: 82,039,747 (GRCm38)	D385G	probably null	Het
Ints1	T	A	5: 139,765,175 (GRCm38)	E824D	probably damaging	Het
Itfg1	T	C	8: 85,835,078 (GRCm38)	D142G	probably benign	Het
Jaml	A	G	9: 45,107,379 (GRCm38)	N359D	probably benign	Het
Kcnh7	T	A	2: 62,850,377 (GRCm38)	I289L	probably benign	Het
Klf12	G	A	14: 100,109,776 (GRCm38)	Q40*	probably null	Het
Mcm4	A	C	16: 15,629,362 (GRCm38)	N579K	possibly damaging	Het
Mfsd2b	A	T	12: 4,867,358 (GRCm38)	F179I	possibly damaging	Het
Mmp11	C	T	10: 75,928,374 (GRCm38)	V86M	probably damaging	Het
Mogs	T	C	6: 83,115,882 (GRCm38)	V101A	probably damaging	Het
Narf	T	A	11: 121,242,626 (GRCm38)	H84Q	probably benign	Het
Olfr987	C	A	2: 85,331,798 (GRCm38)	M33I	probably benign	Het
Parp8	G	A	13: 116,895,115 (GRCm38)	H354Y	possibly damaging	Het
Pcnx	C	A	12: 81,964,480 (GRCm38)	D1238E	probably damaging	Het
Pi4ka	A	G	16: 17,376,982 (GRCm38)	L184P	possibly damaging	Het
Pkd1l3	C	T	8: 109,624,449 (GRCm38)	T642I	probably damaging	Het
Pkhd1l1	G	A	15: 44,589,663 (GRCm38)	E3995K	probably benign	Het
Prdm9	A	T	17: 15,544,956 (GRCm38)	Y521N	probably benign	Het
Prex2	A	G	1: 11,184,456 (GRCm38)	S1105G	probably damaging	Het
Prss35	T	A	9: 86,756,100 (GRCm38)	F308I	probably damaging	Het
Rab22a	C	T	2: 173,701,055 (GRCm38)	A167V	probably benign	Het
Rims2	A	G	15: 39,566,973 (GRCm38)	Q871R	possibly damaging	Het
Rorb	A	T	19: 18,957,247 (GRCm38)	M253K	probably benign	Het
Ryr3	T	C	2: 112,652,610 (GRCm38)	D4269G	probably damaging	Het
Snx11	T	C	11: 96,769,906 (GRCm38)		probably benign	Het
Son	A	G	16: 91,657,188 (GRCm38)	Q941R	probably damaging	Het
Sptbn2	G	T	19: 4,747,785 (GRCm38)	R1880L	probably benign	Het
Sun1	G	A	5: 139,215,259 (GRCm38)		probably null	Het
Tprn	A	G	2: 25,264,038 (GRCm38)	R451G	probably benign	Het
Trbv30	T	A	6: 41,281,377 (GRCm38)	M1K	probably null	Het
Ttn	C	A	2: 76,738,221 (GRCm38)	G25697W	probably damaging	Het
Ubb	T	G	11: 62,551,527 (GRCm38)		probably null	Het
Unc13b	C	T	4: 43,239,331 (GRCm38)	R1038C	probably damaging	Het
Usp7	T	C	16: 8,696,911 (GRCm38)	M687V	probably benign	Het
Zfp160	G	A	17: 21,020,734 (GRCm38)	M21I	probably benign	Het
Zfp868	T	C	8: 69,612,096 (GRCm38)	N196S	probably benign	Het
Zufsp	A	T	10: 33,928,029 (GRCm38)	I483N	probably damaging	Het

Other mutations in Otog

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00516:Otog	APN	7	46,251,282 (GRCm38)	missense	probably damaging	1.00
IGL00725:Otog	APN	7	46,274,092 (GRCm38)	missense	probably damaging	1.00
IGL00757:Otog	APN	7	46,290,128 (GRCm38)	missense	probably damaging	1.00
IGL00822:Otog	APN	7	46,295,880 (GRCm38)	missense	probably benign	0.24
IGL01354:Otog	APN	7	46,289,726 (GRCm38)	missense	probably damaging	1.00
IGL01567:Otog	APN	7	46,276,615 (GRCm38)	splice site	probably benign
IGL02034:Otog	APN	7	46,295,993 (GRCm38)	nonsense	probably null
IGL02090:Otog	APN	7	46,300,147 (GRCm38)	missense	probably damaging	1.00
IGL02132:Otog	APN	7	46,305,479 (GRCm38)	missense	probably damaging	0.99
IGL02148:Otog	APN	7	46,300,587 (GRCm38)	missense	probably damaging	1.00
IGL02173:Otog	APN	7	46,276,741 (GRCm38)	splice site	probably benign
IGL02199:Otog	APN	7	46,277,351 (GRCm38)	missense	possibly damaging	0.90
IGL02216:Otog	APN	7	46,301,468 (GRCm38)	missense	probably damaging	1.00
IGL02322:Otog	APN	7	46,301,457 (GRCm38)	missense	probably benign	0.01
IGL02330:Otog	APN	7	46,288,069 (GRCm38)	missense	possibly damaging	0.84
IGL02529:Otog	APN	7	46,259,957 (GRCm38)	missense	probably damaging	0.99
IGL02898:Otog	APN	7	46,310,138 (GRCm38)	missense	probably damaging	1.00
IGL02970:Otog	APN	7	46,295,867 (GRCm38)	missense	probably benign	0.11
IGL03085:Otog	APN	7	46,305,922 (GRCm38)	critical splice donor site	probably null
IGL03108:Otog	APN	7	46,251,338 (GRCm38)	missense	probably damaging	1.00
IGL03275:Otog	APN	7	46,306,230 (GRCm38)	missense	probably damaging	1.00
R0282_Otog_616	UTSW	7	46,277,493 (GRCm38)	missense	possibly damaging	0.93
R0636_otog_678	UTSW	7	46,264,228 (GRCm38)	critical splice donor site	probably null
R1029_otog_141	UTSW	7	46,274,595 (GRCm38)	missense	probably damaging	1.00
BB010:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
BB020:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
I1329:Otog	UTSW	7	46,246,503 (GRCm38)	missense	probably benign	0.02
IGL02984:Otog	UTSW	7	46,305,508 (GRCm38)	missense	probably damaging	0.98
PIT4472001:Otog	UTSW	7	46,295,849 (GRCm38)	missense	probably damaging	1.00
R0032:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0032:Otog	UTSW	7	46,288,213 (GRCm38)	nonsense	probably null
R0105:Otog	UTSW	7	46,288,366 (GRCm38)	missense	possibly damaging	0.79
R0164:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0164:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0165:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0166:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0167:Otog	UTSW	7	46,304,231 (GRCm38)	missense	probably damaging	0.97
R0240:Otog	UTSW	7	46,264,032 (GRCm38)	splice site	probably null
R0240:Otog	UTSW	7	46,264,032 (GRCm38)	splice site	probably null
R0242:Otog	UTSW	7	46,267,381 (GRCm38)	missense	probably damaging	0.98
R0242:Otog	UTSW	7	46,267,381 (GRCm38)	missense	probably damaging	0.98
R0282:Otog	UTSW	7	46,277,493 (GRCm38)	missense	possibly damaging	0.93
R0392:Otog	UTSW	7	46,250,075 (GRCm38)	missense	probably benign	0.00
R0436:Otog	UTSW	7	46,265,936 (GRCm38)	splice site	probably benign
R0441:Otog	UTSW	7	46,305,877 (GRCm38)	missense	probably damaging	1.00
R0499:Otog	UTSW	7	46,273,832 (GRCm38)	missense	probably damaging	1.00
R0530:Otog	UTSW	7	46,298,244 (GRCm38)	missense	probably damaging	0.98
R0541:Otog	UTSW	7	46,269,249 (GRCm38)	splice site	probably benign
R0600:Otog	UTSW	7	46,251,395 (GRCm38)	splice site	probably benign
R0626:Otog	UTSW	7	46,271,373 (GRCm38)	missense	possibly damaging	0.95
R0636:Otog	UTSW	7	46,264,228 (GRCm38)	critical splice donor site	probably null
R0764:Otog	UTSW	7	46,300,494 (GRCm38)	missense	probably benign	0.00
R0833:Otog	UTSW	7	46,269,362 (GRCm38)	missense	possibly damaging	0.94
R0836:Otog	UTSW	7	46,269,362 (GRCm38)	missense	possibly damaging	0.94
R0844:Otog	UTSW	7	46,287,828 (GRCm38)	missense	possibly damaging	0.53
R1029:Otog	UTSW	7	46,274,595 (GRCm38)	missense	probably damaging	1.00
R1116:Otog	UTSW	7	46,300,601 (GRCm38)	splice site	probably benign
R1134:Otog	UTSW	7	46,298,514 (GRCm38)	missense	probably damaging	1.00
R1183:Otog	UTSW	7	46,289,755 (GRCm38)	missense	probably benign	0.41
R1204:Otog	UTSW	7	46,259,911 (GRCm38)	missense	probably benign	0.16
R1301:Otog	UTSW	7	46,289,689 (GRCm38)	missense	probably damaging	1.00
R1344:Otog	UTSW	7	46,274,615 (GRCm38)	missense	probably damaging	1.00
R1384:Otog	UTSW	7	46,273,695 (GRCm38)	splice site	probably benign
R1418:Otog	UTSW	7	46,274,615 (GRCm38)	missense	probably damaging	1.00
R1432:Otog	UTSW	7	46,300,583 (GRCm38)	missense	probably damaging	1.00
R1479:Otog	UTSW	7	46,295,978 (GRCm38)	missense	possibly damaging	0.75
R1521:Otog	UTSW	7	46,259,264 (GRCm38)	missense	possibly damaging	0.71
R1589:Otog	UTSW	7	46,283,908 (GRCm38)	missense	probably benign	0.18
R1671:Otog	UTSW	7	46,261,786 (GRCm38)	missense	probably damaging	1.00
R1773:Otog	UTSW	7	46,288,159 (GRCm38)	missense	probably benign	0.28
R1806:Otog	UTSW	7	46,290,937 (GRCm38)	critical splice acceptor site	probably null
R1843:Otog	UTSW	7	46,246,283 (GRCm38)	missense	probably damaging	1.00
R1873:Otog	UTSW	7	46,269,343 (GRCm38)	missense	probably damaging	1.00
R1923:Otog	UTSW	7	46,246,283 (GRCm38)	missense	probably damaging	1.00
R1927:Otog	UTSW	7	46,246,283 (GRCm38)	missense	probably damaging	1.00
R2008:Otog	UTSW	7	46,264,074 (GRCm38)	missense	probably benign	0.43
R2048:Otog	UTSW	7	46,287,639 (GRCm38)	missense	probably damaging	1.00
R2131:Otog	UTSW	7	46,250,100 (GRCm38)	missense	probably damaging	1.00
R2153:Otog	UTSW	7	46,302,904 (GRCm38)	missense	probably damaging	1.00
R2240:Otog	UTSW	7	46,241,029 (GRCm38)	start codon destroyed	probably null
R2278:Otog	UTSW	7	46,300,044 (GRCm38)	missense	probably damaging	1.00
R2407:Otog	UTSW	7	46,241,540 (GRCm38)	missense	probably benign	0.10
R2424:Otog	UTSW	7	46,298,169 (GRCm38)	nonsense	probably null
R2513:Otog	UTSW	7	46,305,590 (GRCm38)	critical splice donor site	probably null
R2863:Otog	UTSW	7	46,269,306 (GRCm38)	missense	probably damaging	1.00
R3148:Otog	UTSW	7	46,290,169 (GRCm38)	missense	probably damaging	1.00
R3732:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3732:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3733:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3734:Otog	UTSW	7	46,288,368 (GRCm38)	missense	probably benign	0.03
R3855:Otog	UTSW	7	46,273,760 (GRCm38)	missense	possibly damaging	0.65
R3880:Otog	UTSW	7	46,288,021 (GRCm38)	missense	possibly damaging	0.93
R4081:Otog	UTSW	7	46,288,299 (GRCm38)	missense	possibly damaging	0.92
R4349:Otog	UTSW	7	46,274,189 (GRCm38)	missense	probably damaging	0.99
R4382:Otog	UTSW	7	46,289,698 (GRCm38)	missense	probably damaging	1.00
R4392:Otog	UTSW	7	46,285,124 (GRCm38)	missense	probably damaging	0.98
R4520:Otog	UTSW	7	46,241,053 (GRCm38)	unclassified	probably benign
R4569:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
R4580:Otog	UTSW	7	46,287,801 (GRCm38)	missense	possibly damaging	0.78
R4672:Otog	UTSW	7	46,289,786 (GRCm38)	missense	probably damaging	0.98
R4764:Otog	UTSW	7	46,288,519 (GRCm38)	missense	probably benign	0.29
R4910:Otog	UTSW	7	46,298,534 (GRCm38)	missense	probably damaging	1.00
R4910:Otog	UTSW	7	46,264,062 (GRCm38)	missense	probably damaging	1.00
R4913:Otog	UTSW	7	46,264,102 (GRCm38)	missense	probably benign	0.31
R4975:Otog	UTSW	7	46,287,991 (GRCm38)	missense	probably benign	0.00
R4996:Otog	UTSW	7	46,305,510 (GRCm38)	nonsense	probably null
R4996:Otog	UTSW	7	46,298,606 (GRCm38)	missense	possibly damaging	0.51
R5116:Otog	UTSW	7	46,273,767 (GRCm38)	missense	probably benign	0.34
R5138:Otog	UTSW	7	46,250,006 (GRCm38)	missense	possibly damaging	0.61
R5169:Otog	UTSW	7	46,298,148 (GRCm38)	missense	probably benign	0.06
R5239:Otog	UTSW	7	46,287,435 (GRCm38)	missense	probably benign	0.15
R5277:Otog	UTSW	7	46,246,621 (GRCm38)	missense	possibly damaging	0.89
R5287:Otog	UTSW	7	46,269,329 (GRCm38)	missense	probably damaging	0.98
R5299:Otog	UTSW	7	46,288,851 (GRCm38)	missense	probably benign	0.16
R5378:Otog	UTSW	7	46,255,004 (GRCm38)	missense	probably damaging	1.00
R5382:Otog	UTSW	7	46,249,004 (GRCm38)	missense	probably damaging	1.00
R5487:Otog	UTSW	7	46,288,768 (GRCm38)	missense	probably benign	0.27
R5507:Otog	UTSW	7	46,261,699 (GRCm38)	missense	probably damaging	1.00
R5517:Otog	UTSW	7	46,274,571 (GRCm38)	missense	probably damaging	1.00
R5643:Otog	UTSW	7	46,287,447 (GRCm38)	missense	probably damaging	1.00
R5757:Otog	UTSW	7	46,241,121 (GRCm38)	critical splice donor site	probably null
R5910:Otog	UTSW	7	46,298,598 (GRCm38)	missense	possibly damaging	0.94
R6019:Otog	UTSW	7	46,288,950 (GRCm38)	missense	probably benign	0.00
R6150:Otog	UTSW	7	46,264,059 (GRCm38)	missense	possibly damaging	0.82
R6225:Otog	UTSW	7	46,249,034 (GRCm38)	missense	possibly damaging	0.67
R6271:Otog	UTSW	7	46,252,040 (GRCm38)	missense	probably damaging	1.00
R6317:Otog	UTSW	7	46,301,215 (GRCm38)	missense	probably damaging	1.00
R6454:Otog	UTSW	7	46,305,817 (GRCm38)	missense	probably damaging	1.00
R6640:Otog	UTSW	7	46,261,743 (GRCm38)	missense	possibly damaging	0.92
R6788:Otog	UTSW	7	46,298,317 (GRCm38)	missense	probably damaging	1.00
R6859:Otog	UTSW	7	46,273,781 (GRCm38)	missense	probably damaging	0.96
R7033:Otog	UTSW	7	46,267,398 (GRCm38)	critical splice donor site	probably null
R7071:Otog	UTSW	7	46,267,323 (GRCm38)	missense	probably damaging	1.00
R7084:Otog	UTSW	7	46,298,566 (GRCm38)	nonsense	probably null
R7116:Otog	UTSW	7	46,298,265 (GRCm38)	missense	probably damaging	0.99
R7202:Otog	UTSW	7	46,288,050 (GRCm38)	missense	probably damaging	0.97
R7365:Otog	UTSW	7	46,298,308 (GRCm38)	missense	probably damaging	1.00
R7468:Otog	UTSW	7	46,264,119 (GRCm38)	missense	probably benign
R7475:Otog	UTSW	7	46,267,276 (GRCm38)	missense	probably damaging	0.99
R7502:Otog	UTSW	7	46,298,615 (GRCm38)	missense	probably damaging	1.00
R7558:Otog	UTSW	7	46,303,160 (GRCm38)	missense	probably damaging	0.99
R7577:Otog	UTSW	7	46,287,855 (GRCm38)	missense	possibly damaging	0.62
R7651:Otog	UTSW	7	46,241,761 (GRCm38)	missense	probably benign	0.00
R7689:Otog	UTSW	7	46,252,056 (GRCm38)	missense	probably damaging	1.00
R7806:Otog	UTSW	7	46,285,776 (GRCm38)	missense	probably benign
R7933:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
R8021:Otog	UTSW	7	46,267,342 (GRCm38)	missense	probably damaging	0.98
R8082:Otog	UTSW	7	46,289,719 (GRCm38)	missense	probably damaging	1.00
R8531:Otog	UTSW	7	46,252,049 (GRCm38)	missense	probably damaging	0.99
R8772:Otog	UTSW	7	46,284,928 (GRCm38)	missense	probably damaging	1.00
R8816:Otog	UTSW	7	46,301,481 (GRCm38)	missense	possibly damaging	0.92
R8842:Otog	UTSW	7	46,246,524 (GRCm38)	missense	probably damaging	1.00
R8987:Otog	UTSW	7	46,287,454 (GRCm38)	missense	probably benign	0.43
R8988:Otog	UTSW	7	46,310,147 (GRCm38)	missense	probably damaging	1.00
R9010:Otog	UTSW	7	46,300,470 (GRCm38)	missense	probably benign	0.00
R9025:Otog	UTSW	7	46,288,096 (GRCm38)	missense	probably benign	0.13
R9131:Otog	UTSW	7	46,303,173 (GRCm38)	nonsense	probably null
R9179:Otog	UTSW	7	46,288,461 (GRCm38)	missense	possibly damaging	0.65
R9334:Otog	UTSW	7	46,259,929 (GRCm38)	missense	possibly damaging	0.95
R9365:Otog	UTSW	7	46,271,264 (GRCm38)	missense	probably damaging	1.00
R9408:Otog	UTSW	7	46,267,297 (GRCm38)	missense	possibly damaging	0.79
R9418:Otog	UTSW	7	46,288,600 (GRCm38)	missense	probably benign	0.41
R9465:Otog	UTSW	7	46,305,875 (GRCm38)	missense	possibly damaging	0.80
R9496:Otog	UTSW	7	46,241,081 (GRCm38)	missense	unknown
R9632:Otog	UTSW	7	46,265,719 (GRCm38)	missense	probably benign	0.27
R9656:Otog	UTSW	7	46,310,143 (GRCm38)	missense	probably damaging	1.00
RF024:Otog	UTSW	7	46,287,669 (GRCm38)	missense	probably damaging	1.00
X0062:Otog	UTSW	7	46,259,921 (GRCm38)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	46,289,740 (GRCm38)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	46,274,538 (GRCm38)	missense	probably damaging	1.00
Z1177:Otog	UTSW	7	46,262,852 (GRCm38)	missense	possibly damaging	0.80
Z1177:Otog	UTSW	7	46,309,985 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCTGCCGTGTTGGAGAAAG -3'
(R):5'- ACACACAGGCCAGGTTTGTG -3'

Sequencing Primer
(F):5'- ATGGGGCCACACGATCATC -3'
(R):5'- GTGTTTCTGCCCCAAGGTAG -3'

Posted On

2018-08-01