Mutagenetix > Incidental Mutation

Incidental Mutation 'R6753:Zfp160'

530931

Institutional Source

Beutler Lab

Gene Symbol

Zfp160

Ensembl Gene

ENSMUSG00000067942

Gene Name

zinc finger protein 160

Synonyms

6720480D16Rik, 6720480D16Rik

MMRRC Submission

044870-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6753 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21008903-21043070 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 21020734 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 21 (M21I)

Ref Sequence

ENSEMBL: ENSMUSP00000155916 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088811] [ENSMUST00000231482] [ENSMUST00000232320] [ENSMUST00000232354] [ENSMUST00000232473] [ENSMUST00000232595] [ENSMUST00000232663]

AlphaFold

E9Q459

Predicted Effect

probably benign
Transcript: ENSMUST00000088811
AA Change: M57I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000086191
Gene: ENSMUSG00000067942
AA Change: M57I

Domain	Start	End	E-Value	Type
KRAB	8	68	1.91e-29	SMART
low complexity region	100	110	N/A	INTRINSIC
ZnF_C2H2	146	168	1.69e-3	SMART
ZnF_C2H2	174	196	2.91e-2	SMART
ZnF_C2H2	202	224	1.4e-4	SMART
ZnF_C2H2	230	252	3.89e-3	SMART
ZnF_C2H2	258	280	1.72e-4	SMART
ZnF_C2H2	286	308	4.94e-5	SMART
ZnF_C2H2	314	336	2.12e-4	SMART
ZnF_C2H2	342	364	1.12e-3	SMART
ZnF_C2H2	370	392	1.2e-3	SMART
ZnF_C2H2	398	420	6.42e-4	SMART
ZnF_C2H2	426	448	9.08e-4	SMART
ZnF_C2H2	454	476	1.84e-4	SMART
ZnF_C2H2	482	504	1.5e-4	SMART
ZnF_C2H2	510	532	3.44e-4	SMART
ZnF_C2H2	538	560	1.12e-3	SMART
ZnF_C2H2	566	588	2.27e-4	SMART
ZnF_C2H2	594	616	1.04e-3	SMART
ZnF_C2H2	622	644	1.13e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231482
AA Change: M21I

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

probably benign
Transcript: ENSMUST00000232320
AA Change: M57I

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Predicted Effect

probably benign
Transcript: ENSMUST00000232354
AA Change: M57I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

probably benign
Transcript: ENSMUST00000232473
AA Change: M21I

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

Predicted Effect

probably benign
Transcript: ENSMUST00000232595
AA Change: M57I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000232663
AA Change: M57I

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.1%

Validation Efficiency

99% (68/69)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	G	A	11: 120,010,151	P1083S	probably benign	Het
Abcb5	T	A	12: 118,944,906	N101I	possibly damaging	Het
Adrb2	A	G	18: 62,179,553	V67A	possibly damaging	Het
Agk	T	A	6: 40,368,570		probably null	Het
Akap10	A	T	11: 61,886,777	M586K	probably damaging	Het
Akt3	A	T	1: 177,050,190	Y337*	probably null	Het
Armc1	A	G	3: 19,144,398	F133L	possibly damaging	Het
Bank1	T	C	3: 136,093,308	E424G	probably damaging	Het
Cacna1a	A	G	8: 84,580,205	E1363G	probably damaging	Het
Cacna1d	C	A	14: 30,042,786	A2076S	probably damaging	Het
Ccdc73	T	A	2: 104,991,524	L606*	probably null	Het
Ccdc8	C	T	7: 16,996,637	Q684*	probably null	Het
Ces1b	T	A	8: 93,067,020	K314*	probably null	Het
Ces1e	T	A	8: 93,215,128	N238I	probably damaging	Het
Chd4	A	G	6: 125,114,300	N1238S	probably benign	Het
Cmtr2	T	A	8: 110,222,979	D640E	probably damaging	Het
Col7a1	C	T	9: 108,958,128	T559I	unknown	Het
Comt	T	C	16: 18,408,021	K205R	probably benign	Het
Dbp	A	T	7: 45,708,404	E232V	probably damaging	Het
Dcbld2	A	G	16: 58,456,130	T470A	possibly damaging	Het
Eml6	T	A	11: 29,754,987	D1519V	probably damaging	Het
Evpl	T	A	11: 116,237,906	H31L	possibly damaging	Het
Exoc1	T	A	5: 76,563,339	I86N	probably damaging	Het
Fat3	C	T	9: 15,915,061	E4532K	possibly damaging	Het
Fgfr3	T	C	5: 33,732,159	S301P	probably benign	Het
Gas2l1	C	T	11: 5,064,254	V69I	probably damaging	Het
Gm2042	T	A	12: 87,958,084	I107K	probably damaging	Het
Gucy1b1	T	C	3: 82,039,747	D385G	probably null	Het
Ints1	T	A	5: 139,765,175	E824D	probably damaging	Het
Itfg1	T	C	8: 85,835,078	D142G	probably benign	Het
Jaml	A	G	9: 45,107,379	N359D	probably benign	Het
Kcnh7	T	A	2: 62,850,377	I289L	probably benign	Het
Klf12	G	A	14: 100,109,776	Q40*	probably null	Het
Mcm4	A	C	16: 15,629,362	N579K	possibly damaging	Het
Mfsd2b	A	T	12: 4,867,358	F179I	possibly damaging	Het
Mmp11	C	T	10: 75,928,374	V86M	probably damaging	Het
Mogs	T	C	6: 83,115,882	V101A	probably damaging	Het
Narf	T	A	11: 121,242,626	H84Q	probably benign	Het
Olfr987	C	A	2: 85,331,798	M33I	probably benign	Het
Otog	A	C	7: 46,249,071	E204D	probably benign	Het
Parp8	G	A	13: 116,895,115	H354Y	possibly damaging	Het
Pcnx	C	A	12: 81,964,480	D1238E	probably damaging	Het
Pi4ka	A	G	16: 17,376,982	L184P	possibly damaging	Het
Pkd1l3	C	T	8: 109,624,449	T642I	probably damaging	Het
Pkhd1l1	G	A	15: 44,589,663	E3995K	probably benign	Het
Prdm9	A	T	17: 15,544,956	Y521N	probably benign	Het
Prex2	A	G	1: 11,184,456	S1105G	probably damaging	Het
Prss35	T	A	9: 86,756,100	F308I	probably damaging	Het
Rab22a	C	T	2: 173,701,055	A167V	probably benign	Het
Rims2	A	G	15: 39,566,973	Q871R	possibly damaging	Het
Rorb	A	T	19: 18,957,247	M253K	probably benign	Het
Ryr3	T	C	2: 112,652,610	D4269G	probably damaging	Het
Snx11	T	C	11: 96,769,906		probably benign	Het
Son	A	G	16: 91,657,188	Q941R	probably damaging	Het
Sptbn2	G	T	19: 4,747,785	R1880L	probably benign	Het
Sun1	G	A	5: 139,215,259		probably null	Het
Tprn	A	G	2: 25,264,038	R451G	probably benign	Het
Trbv30	T	A	6: 41,281,377	M1K	probably null	Het
Ttn	C	A	2: 76,738,221	G25697W	probably damaging	Het
Ubb	T	G	11: 62,551,527		probably null	Het
Unc13b	C	T	4: 43,239,331	R1038C	probably damaging	Het
Usp7	T	C	16: 8,696,911	M687V	probably benign	Het
Zfp868	T	C	8: 69,612,096	N196S	probably benign	Het
Zufsp	A	T	10: 33,928,029	I483N	probably damaging	Het

Other mutations in Zfp160

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00714:Zfp160	APN	17	21026702	missense	probably benign
IGL01019:Zfp160	APN	17	21020826	missense	possibly damaging	0.68
IGL02430:Zfp160	APN	17	21025530	missense	possibly damaging	0.76
R0412:Zfp160	UTSW	17	21026877	missense	probably damaging	0.97
R0600:Zfp160	UTSW	17	21027006	missense	probably benign	0.00
R2146:Zfp160	UTSW	17	21026982	missense	probably benign	0.13
R2157:Zfp160	UTSW	17	21020828	missense	probably benign	0.23
R2411:Zfp160	UTSW	17	21025745	missense	possibly damaging	0.94
R2904:Zfp160	UTSW	17	21025649	missense	probably benign	0.00
R4249:Zfp160	UTSW	17	21025738	missense	probably benign	0.11
R4896:Zfp160	UTSW	17	21020081	missense	probably benign	0.00
R5106:Zfp160	UTSW	17	21026761	missense	probably damaging	0.99
R5342:Zfp160	UTSW	17	21020733	missense	possibly damaging	0.95
R5352:Zfp160	UTSW	17	21026852	missense	probably benign	0.02
R6193:Zfp160	UTSW	17	21026862	missense	probably benign	0.24
R6230:Zfp160	UTSW	17	21026445	missense	probably benign	0.38
R6928:Zfp160	UTSW	17	21041462	missense	probably benign	0.04
R7040:Zfp160	UTSW	17	21026532	missense	probably damaging	1.00
R7255:Zfp160	UTSW	17	21025487	missense	probably benign	0.18
R7497:Zfp160	UTSW	17	21026193	missense	probably benign	0.08
R7510:Zfp160	UTSW	17	21026393	missense	probably benign	0.00
R7540:Zfp160	UTSW	17	21025660	nonsense	probably null
R7627:Zfp160	UTSW	17	21027008	missense	probably damaging	0.99
R8169:Zfp160	UTSW	17	21027036	missense	probably damaging	0.97
R8240:Zfp160	UTSW	17	21026088	missense	probably damaging	0.99
R8330:Zfp160	UTSW	17	21026051	missense	probably damaging	1.00
R8367:Zfp160	UTSW	17	21025542	missense	probably benign	0.22
R8802:Zfp160	UTSW	17	21026605	missense	probably damaging	1.00
R9183:Zfp160	UTSW	17	21020092	missense	possibly damaging	0.84
R9556:Zfp160	UTSW	17	21026769	missense	probably benign	0.03
R9695:Zfp160	UTSW	17	21025484	missense	possibly damaging	0.53
Z1177:Zfp160	UTSW	17	21026890	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCAGAAGGAGGCATTCAGTG -3'
(R):5'- ATTACCTGGCACCACATGAG -3'

Sequencing Primer
(F):5'- AAGGAGGCATTCAGTGATGATTTTG -3'
(R):5'- AGTGGCAGACTTATTTCTCACTGAGC -3'

Posted On

2018-08-01