Mutagenetix > Incidental Mutation

Incidental Mutation 'R6754:Vmn1r81'

530958

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r81

Ensembl Gene

ENSMUSG00000115027

Gene Name

vomeronasal 1 receptor 81

Synonyms

V1rg9

Accession Numbers

Essential gene?

Probably essential (E-score: 0.822) question?

Stock #

R6754 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

11993686-11994606 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 11993774 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 278 (I278K)

Ref Sequence

ENSEMBL: ENSMUSP00000153767 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086232] [ENSMUST00000227080] [ENSMUST00000227973] [ENSMUST00000228482] [ENSMUST00000228764]

AlphaFold

A0A2I3BPG7

Predicted Effect

probably damaging
Transcript: ENSMUST00000086232
AA Change: I278K

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000083409
Gene: ENSMUSG00000115027
AA Change: I278K

Domain	Start	End	E-Value	Type
Pfam:TAS2R	3	301	5.4e-12	PFAM
Pfam:V1R	34	299	6.6e-31	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000227080

Predicted Effect

probably damaging
Transcript: ENSMUST00000227973
AA Change: I278K

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

Predicted Effect

probably benign
Transcript: ENSMUST00000228482

Predicted Effect

probably damaging
Transcript: ENSMUST00000228764
AA Change: I278K

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 98.9%
20x: 97.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg3	T	C	17: 57,190,358 (GRCm39)	F352L	probably damaging	Het
Acta2	T	C	19: 34,222,383 (GRCm39)	D246G	probably damaging	Het
Ank2	A	G	3: 126,890,488 (GRCm39)		probably benign	Het
Bmpr2	T	A	1: 59,909,439 (GRCm39)	I971N	probably damaging	Het
C9	C	A	15: 6,519,424 (GRCm39)	C8*	probably null	Het
Casp2	T	A	6: 42,246,264 (GRCm39)	N207K	probably damaging	Het
Cd302	T	G	2: 60,102,451 (GRCm39)	D56A	probably benign	Het
Cecr2	C	T	6: 120,734,539 (GRCm39)	P605S	probably damaging	Het
Cep152	A	G	2: 125,429,588 (GRCm39)	V723A	probably damaging	Het
Col17a1	C	T	19: 47,639,160 (GRCm39)		probably null	Het
Ctsk	T	C	3: 95,409,996 (GRCm39)	V168A	probably damaging	Het
Cyp2c54	A	T	19: 40,060,004 (GRCm39)	N176K	probably damaging	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
F5	A	G	1: 164,021,332 (GRCm39)	D1269G	probably damaging	Het
Fat3	C	T	9: 15,826,357 (GRCm39)	E4532K	possibly damaging	Het
Hivep2	G	A	10: 14,005,382 (GRCm39)	G660D	probably benign	Het
Hspb3	A	T	13: 113,799,688 (GRCm39)	I114K	probably damaging	Het
Irag1	G	A	7: 110,528,719 (GRCm39)	P43S	probably damaging	Het
Itgb7	T	G	15: 102,124,595 (GRCm39)	*807C	probably null	Het
Kbtbd2	A	G	6: 56,756,239 (GRCm39)	V499A	probably damaging	Het
Lemd3	A	T	10: 120,769,565 (GRCm39)	I589N	probably damaging	Het
Mettl13	T	C	1: 162,375,692 (GRCm39)	Y45C	probably damaging	Het
Mllt6	C	T	11: 97,565,273 (GRCm39)	T571I	probably damaging	Het
Nabp1	A	G	1: 51,513,699 (GRCm39)	I99T	probably damaging	Het
Ndufa4	A	G	6: 11,906,052 (GRCm39)	V33A	probably benign	Het
Nr1h5	A	G	3: 102,856,913 (GRCm39)	S200P	probably damaging	Het
Ntsr1	G	A	2: 180,184,476 (GRCm39)	R393H	probably benign	Het
Obi1	C	T	14: 104,740,850 (GRCm39)	E191K	probably damaging	Het
Or14j4	T	C	17: 37,921,046 (GRCm39)	N199D	probably benign	Het
Or4c116	G	T	2: 88,942,640 (GRCm39)	T72N	possibly damaging	Het
Pcdhb21	A	T	18: 37,647,736 (GRCm39)	K288N	probably benign	Het
Pla2g6	T	C	15: 79,190,510 (GRCm39)	N351D	probably benign	Het
Polg	T	C	7: 79,109,584 (GRCm39)	E411G	probably damaging	Het
Prdm11	A	G	2: 92,844,137 (GRCm39)	L31P	probably damaging	Het
Rab27b	G	A	18: 70,129,174 (GRCm39)	T40I	probably damaging	Het
Ruvbl2	T	C	7: 45,078,182 (GRCm39)	D97G	probably benign	Het
Saxo4	A	T	19: 10,454,453 (GRCm39)	Y328N	probably damaging	Het
Sema3a	T	C	5: 13,649,243 (GRCm39)	V664A	possibly damaging	Het
Sh2d3c	T	C	2: 32,644,542 (GRCm39)	S842P	probably damaging	Het
Spam1	A	G	6: 24,796,315 (GRCm39)	T89A	probably damaging	Het
Stab1	C	T	14: 30,863,038 (GRCm39)	G2187E	probably benign	Het
Tff2	C	A	17: 31,363,207 (GRCm39)	A6S	probably benign	Het
Trim80	T	C	11: 115,339,000 (GRCm39)	V610A	probably damaging	Het
Trp53bp1	A	G	2: 121,101,057 (GRCm39)	V47A	possibly damaging	Het
Ufl1	G	A	4: 25,267,796 (GRCm39)	Q292*	probably null	Het
Ulk1	A	T	5: 110,938,259 (GRCm39)	V571E	possibly damaging	Het
Usp24	A	T	4: 106,217,617 (GRCm39)	N447Y	probably damaging	Het

Other mutations in Vmn1r81

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02026:Vmn1r81	APN	7	11,994,432 (GRCm39)	missense	probably damaging	1.00
IGL02086:Vmn1r81	APN	7	11,993,792 (GRCm39)	missense	possibly damaging	0.82
IGL02751:Vmn1r81	APN	7	11,994,374 (GRCm39)	missense	probably damaging	1.00
IGL02879:Vmn1r81	APN	7	11,994,319 (GRCm39)	missense	probably benign	0.20
IGL03358:Vmn1r81	APN	7	11,994,232 (GRCm39)	missense	possibly damaging	0.57
PIT4305001:Vmn1r81	UTSW	7	11,994,590 (GRCm39)	missense	probably benign	0.01
R0359:Vmn1r81	UTSW	7	11,993,877 (GRCm39)	missense	probably damaging	1.00
R1615:Vmn1r81	UTSW	7	11,994,441 (GRCm39)	missense	probably damaging	1.00
R1938:Vmn1r81	UTSW	7	11,994,589 (GRCm39)	missense	possibly damaging	0.67
R2967:Vmn1r81	UTSW	7	11,993,964 (GRCm39)	missense	probably damaging	0.99
R2983:Vmn1r81	UTSW	7	11,994,596 (GRCm39)	missense	probably benign	0.02
R4549:Vmn1r81	UTSW	7	11,993,749 (GRCm39)	missense	probably damaging	0.99
R5099:Vmn1r81	UTSW	7	11,994,248 (GRCm39)	missense	possibly damaging	0.86
R5326:Vmn1r81	UTSW	7	11,994,034 (GRCm39)	missense	probably damaging	1.00
R5542:Vmn1r81	UTSW	7	11,994,034 (GRCm39)	missense	probably damaging	1.00
R6010:Vmn1r81	UTSW	7	11,994,349 (GRCm39)	missense	possibly damaging	0.88
R6630:Vmn1r81	UTSW	7	11,994,584 (GRCm39)	nonsense	probably null
R6724:Vmn1r81	UTSW	7	11,994,599 (GRCm39)	missense	probably damaging	1.00
R8198:Vmn1r81	UTSW	7	11,993,882 (GRCm39)	missense	possibly damaging	0.64
R9719:Vmn1r81	UTSW	7	11,994,449 (GRCm39)	missense	probably damaging	1.00
R9790:Vmn1r81	UTSW	7	11,994,113 (GRCm39)	missense	probably benign	0.15
R9791:Vmn1r81	UTSW	7	11,994,113 (GRCm39)	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- AACAGTGGTCTTAGTGCTCTG -3'
(R):5'- GGCCTGCTCAAGTGTCTCTATG -3'

Sequencing Primer
(F):5'- CAGTGGTCTTAGTGCTCTGCAATTG -3'
(R):5'- AGTCAAGCATATCCATAGTGCTC -3'

Posted On

2018-08-01