Incidental Mutation 'R6760:Calm2'
ID531207
Institutional Source Beutler Lab
Gene Symbol Calm2
Ensembl Gene ENSMUSG00000036438
Gene Namecalmodulin 2
Synonyms
MMRRC Submission
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R6760 (G1)
Quality Score225.009
Status Validated
Chromosome17
Chromosomal Location87433412-87446935 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 87435695 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 65 (D65G)
Ref Sequence ENSEMBL: ENSMUSP00000048857 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000040440]
Predicted Effect probably benign
Transcript: ENSMUST00000040440
AA Change: D65G

PolyPhen 2 Score 0.294 (Sensitivity: 0.91; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000048857
Gene: ENSMUSG00000036438
AA Change: D65G

DomainStartEndE-ValueType
EFh 12 40 6.39e-9 SMART
EFh 48 76 5.19e-9 SMART
EFh 85 113 3.67e-9 SMART
EFh 121 149 1.13e-9 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000143965
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150137
Meta Mutation Damage Score 0.5322 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.5%
  • 10x: 97.6%
  • 20x: 92.8%
Validation Efficiency 97% (35/36)
Allele List at MGI
Other mutations in this stock
Total: 33 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Aco1 C A 4: 40,180,210 C370* probably null Het
Akap2 T C 4: 57,856,026 W493R probably damaging Het
Akap6 C T 12: 53,139,778 S1325L probably damaging Het
Atp2a3 A G 11: 72,982,740 D813G probably damaging Het
Baz2b T A 2: 59,962,432 I451F probably benign Het
Cdh23 C T 10: 60,306,168 V3049M probably damaging Het
Cfap46 A G 7: 139,652,440 L869P probably damaging Het
Chrna9 T A 5: 65,971,228 Y260N probably damaging Het
Clock G A 5: 76,226,976 P782L unknown Het
Coro2a A G 4: 46,540,572 M449T probably benign Het
Crispld1 T G 1: 17,750,801 V355G possibly damaging Het
Dnah7c C A 1: 46,649,340 T1890K probably benign Het
Dnah7c A G 1: 46,649,351 S1894G probably benign Het
Enpp5 G A 17: 44,085,264 G356S probably damaging Het
Gpr37 T C 6: 25,669,169 I559V probably benign Het
Grik5 A G 7: 25,058,939 probably null Het
Itga8 T C 2: 12,301,640 Y48C probably damaging Het
Manba T C 3: 135,542,451 V367A probably damaging Het
Mrgpra1 G A 7: 47,335,041 R297W probably benign Het
Myh7b C A 2: 155,620,118 Y311* probably null Het
Nmd3 T A 3: 69,746,837 probably null Het
Olfr1034 T A 2: 86,047,014 C177* probably null Het
Pcdhb11 A T 18: 37,421,584 probably benign Het
Plcb1 C T 2: 135,472,060 T1144M possibly damaging Het
Sfrp1 A G 8: 23,411,888 D35G probably damaging Het
St3gal1 A G 15: 67,111,346 V187A possibly damaging Het
Timeless A G 10: 128,246,117 K537R probably benign Het
Tnrc6a T A 7: 123,171,999 V1004E probably damaging Het
Tubb4a T C 17: 57,080,796 E410G possibly damaging Het
U2surp G A 9: 95,493,711 A143V probably benign Het
Vmn2r118 T A 17: 55,592,714 H730L possibly damaging Het
Vmn2r28 G A 7: 5,481,230 T657I probably damaging Het
Ybey A T 10: 76,468,199 N56K probably benign Het
Other mutations in Calm2
AlleleSourceChrCoordTypePredicted EffectPPH Score
Prosperous UTSW 17 87435695 missense probably benign 0.29
R0184:Calm2 UTSW 17 87435841 missense probably benign 0.12
R2167:Calm2 UTSW 17 87435145 missense probably benign 0.00
R5452:Calm2 UTSW 17 87435722 splice site probably null
R7298:Calm2 UTSW 17 87442737 splice site probably null
Predicted Primers PCR Primer
(F):5'- GGCACATGACATATGCATCCTC -3'
(R):5'- TCACTATTTGACAAGGACGGG -3'

Sequencing Primer
(F):5'- ATCCTCTGCAGTAAGTTTACACGGG -3'
(R):5'- CTATTTGACAAGGACGGGGATGG -3'
Posted On2018-08-01