|Institutional Source||Beutler Lab|
|Gene Name||RAD52 homolog, DNA repair protein|
|Is this an essential gene?||Possibly non essential (E-score: 0.265)|
|Stock #||R6369 (G1)|
|Chromosomal Location||119902698-119922828 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 119914207 bp|
|Amino Acid Change||Glutamic Acid to Glycine at position 76 (E76G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000125559 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000032269] [ENSMUST00000161045] [ENSMUST00000162461]|
AA Change: E76G
AA Change: E76G
|Coding Region Coverage||
|Validation Efficiency||98% (54/55)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]
PHENOTYPE: Mice homozygous for a null allele exhibit normal reproductive and immune systems. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rad52||
(F):5'- TCACCCAGCAAAATGTGGG -3'
(R):5'- CTTTCTACAGATGAGGGGCAG -3'
(F):5'- CCCAGCAAAATGTGGGTATGTTTC -3'
(R):5'- GATGAGCAAGGATGTTTGAATACTGC -3'