Incidental Mutation 'R6761:Hltf'
| ID |
531690 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Hltf
|
| Ensembl Gene |
ENSMUSG00000002428 |
| Gene Name |
helicase-like transcription factor |
| Synonyms |
Snf2l3, Smarca3, P113 |
| MMRRC Submission |
044877-MU
|
| Accession Numbers |
|
| Essential gene? |
Essential
(E-score: 1.000)
|
| Stock # |
R6761 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
20111975-20172654 bp(+) (GRCm39) |
| Type of Mutation |
critical splice donor site (2 bp from exon) |
| DNA Base Change (assembly) |
T to A
at 20137996 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000002502
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000002502]
[ENSMUST00000143005]
[ENSMUST00000145853]
|
| AlphaFold |
Q6PCN7 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000002502
|
| SMART Domains |
Protein: ENSMUSP00000002502
Gene: ENSMUSG00000002428
| Domain | Start | End | E-Value | Type |
|---|
|
HIRAN
|
60 |
154 |
3.78e-29 |
SMART |
|
DEXDc
|
236 |
608 |
1.26e-32 |
SMART |
|
RING
|
754 |
794 |
4.41e-6 |
SMART |
|
low complexity region
|
814 |
828 |
N/A |
INTRINSIC |
|
HELICc
|
859 |
944 |
2.24e-15 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000143005
|
| SMART Domains |
Protein: ENSMUSP00000116570
Gene: ENSMUSG00000002428
| Domain | Start | End | E-Value | Type |
|---|
|
HIRAN
|
60 |
154 |
3.78e-29 |
SMART |
|
DEXDc
|
236 |
610 |
2.36e-23 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000145853
|
| SMART Domains |
Protein: ENSMUSP00000118775
Gene: ENSMUSG00000002428
| Domain | Start | End | E-Value | Type |
|---|
|
HIRAN
|
1 |
92 |
2.7e-25 |
SMART |
|
DEXDc
|
174 |
548 |
2.36e-23 |
SMART |
|
| Meta Mutation Damage Score |
0.9491 |
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.6%
- 10x: 98.0%
- 20x: 94.5%
|
| Validation Efficiency |
97% (34/35) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit neonatal lethality, spongiform encephalopathy with increased brain apoptosis, and hypoglycemia. Mice homozygous for a different knock-out allele fail to show fluoxetine-induced neurogenesis and behavioral responses. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 32 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 2300002M23Rik |
T |
C |
17: 35,878,845 (GRCm39) |
V61A |
probably benign |
Het |
| Actr3b |
C |
T |
5: 26,030,137 (GRCm39) |
S67F |
probably damaging |
Het |
| Ap3m1 |
T |
C |
14: 21,088,096 (GRCm39) |
M107V |
probably benign |
Het |
| Ccdc27 |
C |
T |
4: 154,122,155 (GRCm39) |
G241D |
unknown |
Het |
| Cit |
T |
G |
5: 116,046,734 (GRCm39) |
D382E |
probably damaging |
Het |
| Clec3b |
G |
T |
9: 122,986,004 (GRCm39) |
G134V |
probably damaging |
Het |
| Cntnap2 |
T |
A |
6: 47,026,307 (GRCm39) |
H44Q |
probably benign |
Het |
| Dst |
T |
C |
1: 34,253,631 (GRCm39) |
S4300P |
probably damaging |
Het |
| Ebna1bp2 |
C |
T |
4: 118,480,558 (GRCm39) |
R134* |
probably null |
Het |
| Efcab14 |
T |
C |
4: 115,596,024 (GRCm39) |
S57P |
probably damaging |
Het |
| Exosc2 |
T |
C |
2: 31,560,875 (GRCm39) |
|
probably null |
Het |
| Hfm1 |
T |
C |
5: 107,043,145 (GRCm39) |
T630A |
probably damaging |
Het |
| Hkdc1 |
A |
T |
10: 62,244,477 (GRCm39) |
I203N |
possibly damaging |
Het |
| Igkv3-2 |
G |
T |
6: 70,675,501 (GRCm39) |
|
probably benign |
Het |
| Mslnl |
A |
G |
17: 25,965,047 (GRCm39) |
D471G |
probably damaging |
Het |
| Msmo1 |
A |
G |
8: 65,172,061 (GRCm39) |
Y281H |
probably benign |
Het |
| Nid1 |
A |
G |
13: 13,656,620 (GRCm39) |
T584A |
probably benign |
Het |
| Olfr908 |
A |
G |
9: 38,427,561 (GRCm39) |
T78A |
probably damaging |
Het |
| Or52a24 |
C |
T |
7: 103,381,691 (GRCm39) |
A186V |
probably damaging |
Het |
| Otoa |
G |
A |
7: 120,721,173 (GRCm39) |
G396D |
probably damaging |
Het |
| Prss45 |
G |
T |
9: 110,669,487 (GRCm39) |
A197S |
probably damaging |
Het |
| Prxl2a |
T |
A |
14: 40,716,578 (GRCm39) |
H198L |
probably damaging |
Het |
| Rpap2 |
T |
A |
5: 107,768,104 (GRCm39) |
I314N |
probably benign |
Het |
| Sash1 |
A |
G |
10: 8,620,286 (GRCm39) |
M458T |
probably damaging |
Het |
| Slc44a5 |
A |
G |
3: 153,945,714 (GRCm39) |
|
probably null |
Het |
| Sorbs2 |
T |
C |
8: 46,225,651 (GRCm39) |
S254P |
probably damaging |
Het |
| Sycp2 |
T |
C |
2: 178,016,144 (GRCm39) |
|
probably null |
Het |
| Tedc1 |
A |
G |
12: 113,125,334 (GRCm39) |
D252G |
probably damaging |
Het |
| Uty |
T |
C |
Y: 1,186,790 (GRCm39) |
H145R |
probably damaging |
Homo |
| Vmn1r9 |
A |
G |
6: 57,048,291 (GRCm39) |
Y122C |
probably benign |
Het |
| Wdfy4 |
T |
A |
14: 32,817,908 (GRCm39) |
N1491Y |
possibly damaging |
Het |
| Wdr37 |
T |
C |
13: 8,899,684 (GRCm39) |
T140A |
probably benign |
Het |
|
| Other mutations in Hltf |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00650:Hltf
|
APN |
3 |
20,159,796 (GRCm39) |
splice site |
probably benign |
|
|
IGL01461:Hltf
|
APN |
3 |
20,154,103 (GRCm39) |
nonsense |
probably null |
|
|
IGL01630:Hltf
|
APN |
3 |
20,137,068 (GRCm39) |
splice site |
probably benign |
|
|
IGL01704:Hltf
|
APN |
3 |
20,137,910 (GRCm39) |
splice site |
probably benign |
|
|
IGL02059:Hltf
|
APN |
3 |
20,160,621 (GRCm39) |
missense |
probably benign |
|
|
IGL02105:Hltf
|
APN |
3 |
20,146,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02156:Hltf
|
APN |
3 |
20,146,971 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
IGL02870:Hltf
|
APN |
3 |
20,154,037 (GRCm39) |
missense |
probably damaging |
0.98 |
|
IGL02899:Hltf
|
APN |
3 |
20,153,981 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02935:Hltf
|
APN |
3 |
20,123,215 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02950:Hltf
|
APN |
3 |
20,130,736 (GRCm39) |
missense |
probably benign |
0.07 |
|
IGL03082:Hltf
|
APN |
3 |
20,118,723 (GRCm39) |
splice site |
probably benign |
|
|
snarky
|
UTSW |
3 |
20,163,651 (GRCm39) |
critical splice donor site |
probably null |
|
|
R0068:Hltf
|
UTSW |
3 |
20,113,254 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0787:Hltf
|
UTSW |
3 |
20,160,610 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0905:Hltf
|
UTSW |
3 |
20,163,033 (GRCm39) |
critical splice donor site |
probably null |
|
|
R0980:Hltf
|
UTSW |
3 |
20,145,665 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1741:Hltf
|
UTSW |
3 |
20,140,352 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1748:Hltf
|
UTSW |
3 |
20,130,685 (GRCm39) |
missense |
probably benign |
0.13 |
|
R1799:Hltf
|
UTSW |
3 |
20,159,855 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1976:Hltf
|
UTSW |
3 |
20,160,610 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2171:Hltf
|
UTSW |
3 |
20,113,245 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2395:Hltf
|
UTSW |
3 |
20,146,906 (GRCm39) |
missense |
probably benign |
0.41 |
|
R2444:Hltf
|
UTSW |
3 |
20,118,071 (GRCm39) |
missense |
possibly damaging |
0.66 |
|
R3789:Hltf
|
UTSW |
3 |
20,123,211 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3943:Hltf
|
UTSW |
3 |
20,146,908 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4719:Hltf
|
UTSW |
3 |
20,118,865 (GRCm39) |
critical splice donor site |
probably null |
|
|
R4793:Hltf
|
UTSW |
3 |
20,118,114 (GRCm39) |
missense |
possibly damaging |
0.79 |
|
R5296:Hltf
|
UTSW |
3 |
20,162,276 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5449:Hltf
|
UTSW |
3 |
20,123,247 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R5492:Hltf
|
UTSW |
3 |
20,152,231 (GRCm39) |
splice site |
probably null |
|
|
R6012:Hltf
|
UTSW |
3 |
20,113,098 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6157:Hltf
|
UTSW |
3 |
20,130,660 (GRCm39) |
missense |
probably benign |
0.13 |
|
R6254:Hltf
|
UTSW |
3 |
20,117,993 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R6553:Hltf
|
UTSW |
3 |
20,126,558 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R6616:Hltf
|
UTSW |
3 |
20,163,651 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6696:Hltf
|
UTSW |
3 |
20,119,470 (GRCm39) |
splice site |
probably null |
|
|
R6781:Hltf
|
UTSW |
3 |
20,152,330 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7241:Hltf
|
UTSW |
3 |
20,119,556 (GRCm39) |
missense |
probably benign |
0.07 |
|
R7356:Hltf
|
UTSW |
3 |
20,163,534 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7453:Hltf
|
UTSW |
3 |
20,136,916 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R7765:Hltf
|
UTSW |
3 |
20,145,647 (GRCm39) |
missense |
probably benign |
0.02 |
|
R7978:Hltf
|
UTSW |
3 |
20,146,968 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8299:Hltf
|
UTSW |
3 |
20,136,986 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
R8547:Hltf
|
UTSW |
3 |
20,152,291 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8857:Hltf
|
UTSW |
3 |
20,159,825 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R8859:Hltf
|
UTSW |
3 |
20,119,566 (GRCm39) |
nonsense |
probably null |
|
|
R8926:Hltf
|
UTSW |
3 |
20,123,323 (GRCm39) |
critical splice donor site |
probably null |
|
|
R8959:Hltf
|
UTSW |
3 |
20,136,936 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9052:Hltf
|
UTSW |
3 |
20,152,246 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9214:Hltf
|
UTSW |
3 |
20,140,280 (GRCm39) |
missense |
probably benign |
0.01 |
|
R9405:Hltf
|
UTSW |
3 |
20,137,094 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9565:Hltf
|
UTSW |
3 |
20,136,996 (GRCm39) |
critical splice donor site |
probably null |
|
|
X0027:Hltf
|
UTSW |
3 |
20,121,553 (GRCm39) |
missense |
probably damaging |
0.96 |
|
| Predicted Primers |
PCR Primer
(F):5'- GTCATAAGTGCCAATGTTGTTTACC -3'
(R):5'- GGCATGGAGAAAGCTACACC -3'
Sequencing Primer
(F):5'- TTCCTAAGGAGGCACAGT -3'
(R):5'- TGCTGTTCATCACCAAAGGAAGTC -3'
|
| Posted On |
2018-08-29 |
Incidental Mutation