Mutagenetix > Incidental Mutation

Incidental Mutation 'R6763:Ceacam14'

531779

Institutional Source

Beutler Lab

Gene Symbol

Ceacam14

Ensembl Gene

ENSMUSG00000023185

Gene Name

CEA cell adhesion molecule 14

Synonyms

1600021E03Rik, 1600025E09Rik

MMRRC Submission

044879-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6763 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

17546607-17549552 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 17549268 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 220 (T220A)

Ref Sequence

ENSEMBL: ENSMUSP00000023953 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023953]

AlphaFold

Q78Y72

Predicted Effect

probably benign
Transcript: ENSMUST00000023953
AA Change: T220A

PolyPhen 2 Score 0.035 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000023953
Gene: ENSMUSG00000023185
AA Change: T220A

Domain	Start	End	E-Value	Type
low complexity region	18	29	N/A	INTRINSIC
IG_like	40	141	6.35e0	SMART
IG_like	158	261	2.73e1	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aaas	C	A	15: 102,248,457 (GRCm39)	R286L	probably null	Het
Actrt2	A	G	4: 154,751,836 (GRCm39)	V100A	probably damaging	Het
Adra1b	A	G	11: 43,666,833 (GRCm39)	L468P	possibly damaging	Het
Ankhd1	A	G	18: 36,776,022 (GRCm39)	E1457G	probably benign	Het
Aspm	A	T	1: 139,398,255 (GRCm39)	M974L	possibly damaging	Het
Atp8a2	T	A	14: 60,245,857 (GRCm39)	I612F	probably benign	Het
Cabin1	A	G	10: 75,582,564 (GRCm39)	L284P	probably damaging	Het
Cand2	T	A	6: 115,776,930 (GRCm39)	M1106K	probably benign	Het
Ccbe1	A	T	18: 66,194,459 (GRCm39)	F376I	possibly damaging	Het
Celsr3	A	G	9: 108,704,549 (GRCm39)	D344G	probably damaging	Het
Chaf1b	A	G	16: 93,688,393 (GRCm39)	K163E	probably damaging	Het
Clec2d	C	A	6: 129,161,107 (GRCm39)	T68K	probably benign	Het
Cwc27	T	C	13: 104,947,809 (GRCm39)	T19A	probably damaging	Het
Dnah7c	A	T	1: 46,668,050 (GRCm39)	Y1519F	possibly damaging	Het
E130308A19Rik	A	T	4: 59,752,288 (GRCm39)	K467M	probably damaging	Het
Garnl3	T	C	2: 32,944,208 (GRCm39)	Y117C	probably damaging	Het
Gas2l3	T	C	10: 89,249,231 (GRCm39)	Y629C	probably benign	Het
Lama1	A	G	17: 68,053,868 (GRCm39)	N470D	unknown	Het
Lmtk2	T	A	5: 144,110,615 (GRCm39)	I445N	probably damaging	Het
Lrba	A	G	3: 86,261,570 (GRCm39)	D1508G	probably damaging	Het
Muc5b	A	G	7: 141,416,021 (GRCm39)	H2989R	probably benign	Het
Niban2	T	C	2: 32,801,460 (GRCm39)		probably null	Het
Nln	A	T	13: 104,172,163 (GRCm39)	W638R	probably damaging	Het
Nup133	T	C	8: 124,671,017 (GRCm39)	I127V	possibly damaging	Het
Nup155	C	T	15: 8,165,379 (GRCm39)	R672*	probably null	Het
Prkcb	A	G	7: 122,193,887 (GRCm39)	Y532C	probably damaging	Het
Ptpro	A	G	6: 137,395,279 (GRCm39)		probably null	Het
Rab11fip5	A	G	6: 85,319,152 (GRCm39)	L579S	probably benign	Het
Rtca	A	G	3: 116,301,398 (GRCm39)		probably null	Het
Sdccag8	A	G	1: 176,682,193 (GRCm39)		probably null	Het
Svil	G	T	18: 5,056,437 (GRCm39)	D524Y	probably damaging	Het
Unc80	A	G	1: 66,560,636 (GRCm39)	N788S	probably benign	Het
Wdfy4	G	T	14: 32,764,469 (GRCm39)	R2140S	probably damaging	Het
Zfp518a	A	G	19: 40,902,192 (GRCm39)	K707R	probably damaging	Het

Other mutations in Ceacam14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Ceacam14	APN	7	17,548,062 (GRCm39)	missense	probably damaging	1.00
R0452:Ceacam14	UTSW	7	17,549,248 (GRCm39)	missense	probably benign
R1613:Ceacam14	UTSW	7	17,547,973 (GRCm39)	splice site	probably benign
R1990:Ceacam14	UTSW	7	17,549,290 (GRCm39)	nonsense	probably null
R2153:Ceacam14	UTSW	7	17,548,153 (GRCm39)	missense	probably benign	0.00
R3887:Ceacam14	UTSW	7	17,548,063 (GRCm39)	missense	probably damaging	1.00
R4724:Ceacam14	UTSW	7	17,547,975 (GRCm39)	critical splice acceptor site	probably null
R5323:Ceacam14	UTSW	7	17,549,402 (GRCm39)	makesense	probably null
R5454:Ceacam14	UTSW	7	17,548,110 (GRCm39)	missense	probably damaging	1.00
R5696:Ceacam14	UTSW	7	17,548,267 (GRCm39)	missense	probably damaging	0.97
R6318:Ceacam14	UTSW	7	17,548,237 (GRCm39)	missense	probably damaging	1.00
R7607:Ceacam14	UTSW	7	17,548,246 (GRCm39)	missense	possibly damaging	0.95
R7789:Ceacam14	UTSW	7	17,548,096 (GRCm39)	missense	probably damaging	0.99
R8946:Ceacam14	UTSW	7	17,547,999 (GRCm39)	missense	probably benign	0.01
R9781:Ceacam14	UTSW	7	17,549,082 (GRCm39)	missense	possibly damaging	0.60

Predicted Primers

PCR Primer
(F):5'- CAAAATTGCGTTGGTGCCAC -3'
(R):5'- AGGTAGAATAGAAACCTCACTGTC -3'

Sequencing Primer
(F):5'- GTGCCACCTAGGGTTGAAG -3'
(R):5'- ACCTCACTGTCTTAAGTTTACAGAG -3'

Posted On

2018-08-29