Mutagenetix > Incidental Mutation

Incidental Mutation 'R6768:Vmn2r61'

532043

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r61

Ensembl Gene

ENSMUSG00000090967

Gene Name

vomeronasal 2, receptor 61

Synonyms

Gprc2a-rs2, Casr-rs2, EG637873

MMRRC Submission

044884-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R6768 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41909477-41950179 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 41949748 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 723 (P723S)

Ref Sequence

ENSEMBL: ENSMUSP00000129576 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166131]

AlphaFold

L7N2B8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166131
AA Change: P723S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000129576
Gene: ENSMUSG00000090967
AA Change: P723S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	4e-42	PFAM
Pfam:NCD3G	514	567	1.9e-21	PFAM
Pfam:7tm_3	600	835	6.2e-52	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 98.9%
20x: 97.2%

Validation Efficiency

96% (45/47)

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam6b	G	A	12: 113,453,863 (GRCm39)	V227I	probably benign	Het
Agtrap	A	G	4: 148,166,067 (GRCm39)	V106A	probably benign	Het
Aldh3a2	A	C	11: 61,144,536 (GRCm39)	S341A	probably benign	Het
Bmper	T	A	9: 23,292,749 (GRCm39)	C353S	probably damaging	Het
Casq1	C	T	1: 172,047,245 (GRCm39)	D5N	probably benign	Het
Ccin	G	A	4: 43,984,574 (GRCm39)	R327H	probably benign	Het
Chuk	A	G	19: 44,085,390 (GRCm39)	V252A	probably damaging	Het
Colec11	T	C	12: 28,645,100 (GRCm39)		probably null	Het
Cpeb3	G	A	19: 37,002,432 (GRCm39)	T643I	possibly damaging	Het
Cstpp1	A	T	2: 91,252,303 (GRCm39)	H19Q	probably damaging	Het
Ctcfl	A	G	2: 172,959,084 (GRCm39)	V214A	possibly damaging	Het
Dst	A	G	1: 34,220,793 (GRCm39)	E2199G	probably damaging	Het
Eno2	T	C	6: 124,744,711 (GRCm39)	E45G	probably damaging	Het
Foxa2	T	C	2: 147,885,747 (GRCm39)	H181R	probably damaging	Het
Fpgs	A	G	2: 32,576,635 (GRCm39)	S331P	probably benign	Het
Gm47189	A	G	14: 41,492,035 (GRCm39)	S81P	probably benign	Het
Igsf8	C	A	1: 172,145,099 (GRCm39)	P142Q	probably damaging	Het
Islr	G	T	9: 58,064,893 (GRCm39)	Q205K	possibly damaging	Het
Josd1	A	G	15: 79,561,323 (GRCm39)	W162R	probably benign	Het
Lrrc37a	G	A	11: 103,390,949 (GRCm39)	T1492I	probably benign	Het
Meox1	A	G	11: 101,770,161 (GRCm39)	F189L	probably damaging	Het
Mtf1	G	A	4: 124,731,578 (GRCm39)	D385N	probably benign	Het
Naip2	A	T	13: 100,314,832 (GRCm39)	C315*	probably null	Het
Ncaph2	T	A	15: 89,248,202 (GRCm39)	Y166*	probably null	Het
Nr1i3	G	A	1: 171,044,966 (GRCm39)	V270M	probably damaging	Het
Or11h4b	A	G	14: 50,919,049 (GRCm39)	L14S	probably damaging	Het
Or13c7	T	C	4: 43,854,351 (GRCm39)	I14T	probably benign	Het
Panx3	T	C	9: 37,575,322 (GRCm39)	K180R	probably benign	Het
Pi4ka	A	G	16: 17,194,846 (GRCm39)	L184P	possibly damaging	Het
Reln	A	C	5: 22,183,905 (GRCm39)	V1698G	probably damaging	Het
Rnf213	G	T	11: 119,333,062 (GRCm39)	R2757L	probably damaging	Het
Scgb2b26	G	T	7: 33,644,379 (GRCm39)	T4K	probably damaging	Het
Sdhb	A	G	4: 140,706,364 (GRCm39)	E267G	probably damaging	Het
Sorbs1	A	T	19: 40,315,991 (GRCm39)	N383K	probably damaging	Het
Stk11ip	T	A	1: 75,509,279 (GRCm39)	C766S	probably benign	Het
Taf6l	A	C	19: 8,751,913 (GRCm39)	S592A	probably damaging	Het
Tmem145	G	A	7: 25,008,061 (GRCm39)	G235D	probably damaging	Het
Tuba3b	T	A	6: 145,564,455 (GRCm39)		probably null	Het
Ubl7	A	T	9: 57,820,045 (GRCm39)	E32D	probably benign	Het
Vmn1r168	A	T	7: 23,240,460 (GRCm39)	T106S	probably damaging	Het
Vmn2r59	C	A	7: 41,661,392 (GRCm39)	V808F	probably benign	Het
Wwc2	A	G	8: 48,353,826 (GRCm39)	Y103H	possibly damaging	Het
Zfp11	G	T	5: 129,735,415 (GRCm39)	D15E	probably benign	Het
Zfp428	G	A	7: 24,214,908 (GRCm39)	G162R	probably damaging	Het
Zfp629	G	A	7: 127,209,997 (GRCm39)	T604I	probably benign	Het
Zfpm1	C	A	8: 123,061,195 (GRCm39)	D253E	probably damaging	Het
Zhx1	G	T	15: 57,917,499 (GRCm39)	T249K	probably benign	Het

Other mutations in Vmn2r61

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r61	APN	7	41,950,175 (GRCm39)	missense	possibly damaging	0.96
IGL00824:Vmn2r61	APN	7	41,916,438 (GRCm39)	missense	probably benign	0.03
IGL00903:Vmn2r61	APN	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
IGL01125:Vmn2r61	APN	7	41,909,550 (GRCm39)	missense	probably damaging	1.00
IGL01393:Vmn2r61	APN	7	41,916,258 (GRCm39)	missense	probably benign	0.08
IGL01712:Vmn2r61	APN	7	41,909,661 (GRCm39)	missense	probably damaging	0.98
IGL01822:Vmn2r61	APN	7	41,950,130 (GRCm39)	missense	probably benign	0.18
IGL01835:Vmn2r61	APN	7	41,950,015 (GRCm39)	missense	probably benign	0.12
IGL01844:Vmn2r61	APN	7	41,909,639 (GRCm39)	missense	probably benign	0.00
IGL01953:Vmn2r61	APN	7	41,949,613 (GRCm39)	missense	probably damaging	1.00
IGL02032:Vmn2r61	APN	7	41,949,466 (GRCm39)	missense	probably damaging	0.99
IGL02054:Vmn2r61	APN	7	41,926,158 (GRCm39)	critical splice donor site	probably null
IGL02569:Vmn2r61	APN	7	41,926,070 (GRCm39)	missense	probably damaging	1.00
IGL02697:Vmn2r61	APN	7	41,924,892 (GRCm39)	missense	possibly damaging	0.55
IGL02958:Vmn2r61	APN	7	41,949,361 (GRCm39)	missense	probably benign
IGL03290:Vmn2r61	APN	7	41,915,408 (GRCm39)	missense	probably benign	0.00
IGL03337:Vmn2r61	APN	7	41,916,509 (GRCm39)	missense	possibly damaging	0.58
IGL03369:Vmn2r61	APN	7	41,909,517 (GRCm39)	missense	probably benign
IGL03402:Vmn2r61	APN	7	41,909,679 (GRCm39)	missense	probably benign
R0026:Vmn2r61	UTSW	7	41,924,898 (GRCm39)	missense	possibly damaging	0.64
R0319:Vmn2r61	UTSW	7	41,949,941 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r61	UTSW	7	41,915,335 (GRCm39)	missense	probably benign	0.02
R0555:Vmn2r61	UTSW	7	41,915,442 (GRCm39)	missense	probably benign	0.02
R0691:Vmn2r61	UTSW	7	41,949,844 (GRCm39)	missense	probably damaging	1.00
R1701:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R1718:Vmn2r61	UTSW	7	41,950,121 (GRCm39)	missense	probably benign
R1835:Vmn2r61	UTSW	7	41,916,076 (GRCm39)	nonsense	probably null
R1920:Vmn2r61	UTSW	7	41,949,710 (GRCm39)	missense	possibly damaging	0.73
R2069:Vmn2r61	UTSW	7	41,949,425 (GRCm39)	missense	probably benign	0.06
R2326:Vmn2r61	UTSW	7	41,916,287 (GRCm39)	missense	probably damaging	1.00
R2402:Vmn2r61	UTSW	7	41,949,529 (GRCm39)	missense	possibly damaging	0.90
R3103:Vmn2r61	UTSW	7	41,916,067 (GRCm39)	missense	possibly damaging	0.73
R3107:Vmn2r61	UTSW	7	41,916,491 (GRCm39)	missense	possibly damaging	0.82
R4426:Vmn2r61	UTSW	7	41,950,159 (GRCm39)	missense	probably benign
R4426:Vmn2r61	UTSW	7	41,950,157 (GRCm39)	missense	probably benign
R4484:Vmn2r61	UTSW	7	41,950,120 (GRCm39)	missense	probably benign
R4748:Vmn2r61	UTSW	7	41,916,565 (GRCm39)	missense	probably damaging	0.96
R4835:Vmn2r61	UTSW	7	41,916,459 (GRCm39)	missense	possibly damaging	0.52
R4863:Vmn2r61	UTSW	7	41,950,132 (GRCm39)	missense	probably benign	0.03
R4923:Vmn2r61	UTSW	7	41,916,520 (GRCm39)	missense	probably damaging	1.00
R4968:Vmn2r61	UTSW	7	41,949,478 (GRCm39)	missense	probably benign	0.14
R5114:Vmn2r61	UTSW	7	41,949,953 (GRCm39)	missense	possibly damaging	0.92
R5297:Vmn2r61	UTSW	7	41,909,646 (GRCm39)	missense	probably benign
R5497:Vmn2r61	UTSW	7	41,924,906 (GRCm39)	missense	possibly damaging	0.95
R5508:Vmn2r61	UTSW	7	41,916,242 (GRCm39)	missense	possibly damaging	0.52
R5587:Vmn2r61	UTSW	7	41,949,911 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
R5615:Vmn2r61	UTSW	7	41,916,677 (GRCm39)	missense	probably benign	0.00
R5782:Vmn2r61	UTSW	7	41,949,253 (GRCm39)	missense	probably damaging	1.00
R6136:Vmn2r61	UTSW	7	41,916,455 (GRCm39)	missense	probably damaging	1.00
R6207:Vmn2r61	UTSW	7	41,909,616 (GRCm39)	missense	probably benign	0.01
R6265:Vmn2r61	UTSW	7	41,915,915 (GRCm39)	missense	probably benign	0.01
R6272:Vmn2r61	UTSW	7	41,949,242 (GRCm39)	missense	probably damaging	1.00
R6355:Vmn2r61	UTSW	7	41,916,659 (GRCm39)	missense	probably benign	0.00
R6469:Vmn2r61	UTSW	7	41,915,283 (GRCm39)	nonsense	probably null
R6554:Vmn2r61	UTSW	7	41,926,139 (GRCm39)	missense	probably damaging	1.00
R6699:Vmn2r61	UTSW	7	41,949,580 (GRCm39)	missense	probably benign
R6824:Vmn2r61	UTSW	7	41,949,403 (GRCm39)	missense	probably benign	0.10
R6930:Vmn2r61	UTSW	7	41,949,364 (GRCm39)	missense	probably benign	0.02
R7053:Vmn2r61	UTSW	7	41,916,557 (GRCm39)	missense	probably damaging	0.96
R7238:Vmn2r61	UTSW	7	41,916,629 (GRCm39)	missense	possibly damaging	0.73
R7332:Vmn2r61	UTSW	7	41,909,534 (GRCm39)	missense	probably benign	0.00
R7359:Vmn2r61	UTSW	7	41,915,407 (GRCm39)	missense	probably benign	0.11
R7553:Vmn2r61	UTSW	7	41,916,205 (GRCm39)	missense	not run
R7710:Vmn2r61	UTSW	7	41,916,472 (GRCm39)	missense	probably damaging	1.00
R7732:Vmn2r61	UTSW	7	41,916,097 (GRCm39)	missense	probably benign
R7839:Vmn2r61	UTSW	7	41,916,032 (GRCm39)	missense	probably damaging	0.97
R7916:Vmn2r61	UTSW	7	41,949,935 (GRCm39)	missense	probably damaging	1.00
R8026:Vmn2r61	UTSW	7	41,916,141 (GRCm39)	missense	probably benign	0.02
R8440:Vmn2r61	UTSW	7	41,916,080 (GRCm39)	missense	probably benign	0.02
R8499:Vmn2r61	UTSW	7	41,949,700 (GRCm39)	missense	probably damaging	0.99
R8771:Vmn2r61	UTSW	7	41,916,194 (GRCm39)	missense	probably damaging	0.99
R8847:Vmn2r61	UTSW	7	41,950,010 (GRCm39)	missense	probably damaging	1.00
R8986:Vmn2r61	UTSW	7	41,915,325 (GRCm39)	nonsense	probably null
R9290:Vmn2r61	UTSW	7	41,915,385 (GRCm39)	missense	probably benign	0.27
R9311:Vmn2r61	UTSW	7	41,950,092 (GRCm39)	missense	possibly damaging	0.92
R9324:Vmn2r61	UTSW	7	41,916,619 (GRCm39)	missense	probably benign	0.00
R9476:Vmn2r61	UTSW	7	41,949,593 (GRCm39)	missense	probably damaging	1.00
R9521:Vmn2r61	UTSW	7	41,916,626 (GRCm39)	missense	probably damaging	0.99
R9619:Vmn2r61	UTSW	7	41,926,136 (GRCm39)	missense	probably damaging	0.98
R9729:Vmn2r61	UTSW	7	41,949,917 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r61	UTSW	7	41,949,388 (GRCm39)	missense	possibly damaging	0.93
Z1176:Vmn2r61	UTSW	7	41,916,166 (GRCm39)	missense	probably benign	0.00
Z1176:Vmn2r61	UTSW	7	41,909,585 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GACACACCCATTGTCAAGGC -3'
(R):5'- TGGCCTCATTGAAGGTGTCAG -3'

Sequencing Primer
(F):5'- AGGACTCTCAGCTACATCCTG -3'
(R):5'- CATTGAAGGTGTCAGGCAGATTCC -3'

Posted On

2018-08-29