Mutagenetix > Incidental Mutation

Incidental Mutation 'R6746:Mrgprb1'

532834

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb1

Ensembl Gene

ENSMUSG00000070547

Gene Name

MAS-related GPR, member B1

Synonyms

MrgB1

MMRRC Submission

044863-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R6746 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

48444113-48456342 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 48447897 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glycine at position 89 (V89G)

Ref Sequence

ENSEMBL: ENSMUSP00000091946 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094384] [ENSMUST00000188095] [ENSMUST00000188918]

AlphaFold

Q3UG61

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094384
AA Change: V89G

PolyPhen 2 Score 0.816 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000091946
Gene: ENSMUSG00000070547
AA Change: V89G

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	50	227	5.5e-11	PFAM
Pfam:7tm_1	59	290	4.3e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188095

Predicted Effect

probably benign
Transcript: ENSMUST00000188918

SMART Domains

Protein: ENSMUSP00000140432
Gene: ENSMUSG00000070547

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	23	84	3e-6	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.3%

Validation Efficiency

100% (58/58)

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	T	17: 24,346,221 (GRCm38)	L79*	probably null	Het
Acot3	A	G	12: 84,053,474 (GRCm38)	N8S	probably benign	Het
Adora2a	T	C	10: 75,333,608 (GRCm38)	V302A	probably benign	Het
Anpep	A	C	7: 79,839,185 (GRCm38)		probably null	Het
Arrb1	A	G	7: 99,601,150 (GRCm38)	K392E	probably benign	Het
Atp8a1	A	T	5: 67,751,049 (GRCm38)	N444K	probably benign	Het
Brinp2	C	T	1: 158,266,590 (GRCm38)	G181R	probably benign	Het
Cacna1g	A	T	11: 94,409,427 (GRCm38)	C2184*	probably null	Het
Cacna1h	C	T	17: 25,381,550 (GRCm38)	A1606T	probably damaging	Het
Ccdc155	C	T	7: 45,200,311 (GRCm38)	V63I	probably benign	Het
Ccdc30	T	C	4: 119,356,718 (GRCm38)	T205A	probably benign	Het
Celsr1	A	G	15: 86,031,495 (GRCm38)	I759T	probably damaging	Het
Chaf1a	A	G	17: 56,063,404 (GRCm38)	D623G	possibly damaging	Het
Col6a3	G	T	1: 90,779,045 (GRCm38)	N2115K	unknown	Het
Dync1h1	A	T	12: 110,651,653 (GRCm38)	T3209S	probably damaging	Het
Erich6	T	C	3: 58,616,566 (GRCm38)	D629G	possibly damaging	Het
Fahd1	G	T	17: 24,849,941 (GRCm38)	A54E	probably damaging	Het
Flrt3	C	T	2: 140,660,025 (GRCm38)	R561Q	probably damaging	Het
Gm5415	A	T	1: 32,546,763 (GRCm38)	I22N	probably benign	Het
Grm6	A	T	11: 50,856,963 (GRCm38)	D334V	probably damaging	Het
Helb	A	T	10: 120,105,468 (GRCm38)	D438E	probably damaging	Het
Hmgcs1	T	C	13: 119,695,049 (GRCm38)		probably null	Het
Hnf4g	C	A	3: 3,657,110 (GRCm38)	Y441*	probably null	Het
Hrasls5	A	G	19: 7,613,330 (GRCm38)	D74G	probably benign	Het
Hspa13	A	T	16: 75,765,037 (GRCm38)	N91K	possibly damaging	Het
Ilvbl	T	C	10: 78,577,223 (GRCm38)	I193T	possibly damaging	Het
Itga7	T	C	10: 128,949,472 (GRCm38)	V848A	probably benign	Het
Lpin1	A	G	12: 16,565,528 (GRCm38)	M341T	probably benign	Het
Lypd5	G	T	7: 24,353,106 (GRCm38)		probably null	Het
Nsun7	T	C	5: 66,283,737 (GRCm38)		probably null	Het
Oasl1	T	A	5: 114,937,183 (GRCm38)	V434E	probably damaging	Het
Olfr1008	T	C	2: 85,689,608 (GRCm38)	Y60H	probably damaging	Het
Olfr319	G	A	11: 58,702,543 (GRCm38)	V281I	probably benign	Het
Olfr837	A	G	9: 19,137,478 (GRCm38)	M162V	probably benign	Het
Otor	T	A	2: 143,080,035 (GRCm38)		probably null	Het
Pik3cg	G	A	12: 32,194,758 (GRCm38)	T899M	probably damaging	Het
Pld2	C	A	11: 70,541,107 (GRCm38)	L52M	probably damaging	Het
Pon3	A	T	6: 5,230,786 (GRCm38)	M247K	possibly damaging	Het
Ppfia2	C	A	10: 106,906,458 (GRCm38)	Y1037*	probably null	Het
Ppm1m	A	T	9: 106,198,152 (GRCm38)	C99*	probably null	Het
Prss44	A	T	9: 110,815,293 (GRCm38)	*145C	probably null	Het
Ptpro	T	A	6: 137,394,823 (GRCm38)	Y613N	probably damaging	Het
Ralgapb	T	G	2: 158,476,136 (GRCm38)	V866G	probably damaging	Het
Rassf6	C	A	5: 90,609,774 (GRCm38)	R109L	possibly damaging	Het
Rbm4b	A	C	19: 4,762,003 (GRCm38)	T147P	probably benign	Het
Rpl7l1	T	C	17: 46,779,396 (GRCm38)	K104R	probably benign	Het
Ryr1	A	T	7: 29,117,404 (GRCm38)	I69N	possibly damaging	Het
Scd1	G	T	19: 44,406,488 (GRCm38)	F99L	probably benign	Het
Spint2	A	T	7: 29,259,423 (GRCm38)	S66T	probably benign	Het
Tarm1	T	A	7: 3,502,462 (GRCm38)	I2F	probably benign	Het
Tenm4	T	C	7: 96,892,860 (GRCm38)	V1860A	probably damaging	Het
Uhrf1bp1l	T	A	10: 89,787,158 (GRCm38)	N298K	probably benign	Het
Usp38	A	G	8: 81,014,291 (GRCm38)	I49T	possibly damaging	Het
Vars2	A	G	17: 35,660,402 (GRCm38)		probably null	Het
Vmn2r27	T	G	6: 124,200,593 (GRCm38)	H484P	possibly damaging	Het
Wdr35	T	A	12: 9,003,982 (GRCm38)		probably null	Het
Zfp937	A	T	2: 150,239,423 (GRCm38)	K458*	probably null	Het

Other mutations in Mrgprb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00540:Mrgprb1	APN	7	48,447,543 (GRCm38)	missense	probably damaging	0.99
IGL01141:Mrgprb1	APN	7	48,448,027 (GRCm38)	missense	probably benign	0.36
IGL01393:Mrgprb1	APN	7	48,448,006 (GRCm38)	missense	possibly damaging	0.48
IGL02430:Mrgprb1	APN	7	48,447,661 (GRCm38)	missense	possibly damaging	0.95
IGL02485:Mrgprb1	APN	7	48,447,717 (GRCm38)	missense	possibly damaging	0.88
R0026:Mrgprb1	UTSW	7	48,447,204 (GRCm38)	missense	possibly damaging	0.66
R0051:Mrgprb1	UTSW	7	48,447,214 (GRCm38)	missense	probably benign	0.01
R0789:Mrgprb1	UTSW	7	48,456,184 (GRCm38)	splice site	probably benign
R1223:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R1327:Mrgprb1	UTSW	7	48,447,429 (GRCm38)	missense	possibly damaging	0.87
R1456:Mrgprb1	UTSW	7	48,448,029 (GRCm38)	missense	probably damaging	0.98
R1561:Mrgprb1	UTSW	7	48,447,125 (GRCm38)	splice site	probably null
R1567:Mrgprb1	UTSW	7	48,447,453 (GRCm38)	missense	probably damaging	0.97
R2030:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.83
R2165:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.00
R2885:Mrgprb1	UTSW	7	48,447,721 (GRCm38)	missense	probably damaging	1.00
R3108:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.93
R3919:Mrgprb1	UTSW	7	48,448,081 (GRCm38)	missense	probably benign	0.03
R4021:Mrgprb1	UTSW	7	48,447,123 (GRCm38)	missense	possibly damaging	0.95
R4613:Mrgprb1	UTSW	7	48,447,708 (GRCm38)	missense	possibly damaging	0.91
R4809:Mrgprb1	UTSW	7	48,447,991 (GRCm38)	missense	possibly damaging	0.89
R5249:Mrgprb1	UTSW	7	48,447,477 (GRCm38)	missense	possibly damaging	0.91
R5425:Mrgprb1	UTSW	7	48,447,971 (GRCm38)	missense	possibly damaging	0.81
R5555:Mrgprb1	UTSW	7	48,447,775 (GRCm38)	missense	probably benign	0.06
R5595:Mrgprb1	UTSW	7	48,447,684 (GRCm38)	missense	probably damaging	0.99
R5982:Mrgprb1	UTSW	7	48,447,820 (GRCm38)	missense	probably benign	0.01
R7066:Mrgprb1	UTSW	7	48,447,676 (GRCm38)	missense	probably benign	0.27
R7141:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R7633:Mrgprb1	UTSW	7	48,447,583 (GRCm38)	missense	probably benign	0.01
R8072:Mrgprb1	UTSW	7	48,448,147 (GRCm38)	nonsense	probably null
R8080:Mrgprb1	UTSW	7	48,446,910 (GRCm38)	splice site	probably null
R8112:Mrgprb1	UTSW	7	48,447,934 (GRCm38)	missense	probably damaging	0.97
R8493:Mrgprb1	UTSW	7	48,447,573 (GRCm38)	missense	probably damaging	0.99
R8817:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.01
R9135:Mrgprb1	UTSW	7	48,447,298 (GRCm38)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TGGGCCACATGACAGATAGG -3'
(R):5'- ATTGCACCACTCCTGAAAATG -3'

Sequencing Primer
(F):5'- GAAGCGTTCAGTGCTAATGAC -3'
(R):5'- CTCCTGAAAATGGACTTAGTCATCC -3'

Posted On

2018-08-29