|Institutional Source||Beutler Lab|
|Gene Name||tripartite motif-containing 68|
|Synonyms||Rnf137, F730114J12Rik, SS-56|
|Is this an essential gene?||Probably non essential (E-score: 0.063)|
|Stock #||R6749 (G1)|
|Chromosomal Location||102677582-102687327 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 102678783 bp|
|Amino Acid Change||Aspartic acid to Glycine at position 321 (D321G)|
|Ref Sequence||ENSEMBL: ENSMUSP00000080813 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000082175] [ENSMUST00000210855]|
|Predicted Effect||probably damaging
AA Change: D321G
PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)
AA Change: D321G
|Coding Region Coverage||
|Validation Efficiency||100% (47/47)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the tripartite motif-containing protein family, whose members are characterized by a "really interesting new gene" (RING) finger domain, a zinc-binding B-box motif, and a coiled-coil region. Members of this family function as E3 ubiquitin ligases and are involved in a broad range of biological processes. This gene regulates the activation of nuclear receptors, such as androgen receptor, and has been implicated in development of prostate cancer cells, where its expression increases in response to a downregulation of microRNAs. In addition, this gene participates in viral defense regulation as a negative regulator of interferon-beta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Trim68||
(F):5'- AGGGGATAAGTACACCACCTCC -3'
(R):5'- AATGGCTCAGTGCAATCTGG -3'
(F):5'- GATAAGTACACCACCTCCTTCCGG -3'
(R):5'- CAGTGCAATCTGGATGCTTCATCTAG -3'