Mutagenetix > Incidental Mutation

Incidental Mutation 'R6800:Zfp87'

533297

Institutional Source

Beutler Lab

Gene Symbol

Zfp87

Ensembl Gene

ENSMUSG00000097333

Gene Name

zinc finger protein 87

Synonyms

Mzf22, 2210039O17Rik, 4631412O18Rik, Zfp72, KRAB4

MMRRC Submission

044913-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R6800 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67663900-67674296 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 74520080 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 333 (S333G)

Ref Sequence

ENSEMBL: ENSMUSP00000152502 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091481] [ENSMUST00000223163]

AlphaFold

Q8K2A4

Predicted Effect

probably benign
Transcript: ENSMUST00000091481
AA Change: S333G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000089063
Gene: ENSMUSG00000069184
AA Change: S333G

Domain	Start	End	E-Value	Type
KRAB	1	32	3.57e-1	SMART
ZnF_C2H2	48	70	5.42e-2	SMART
ZnF_C2H2	76	98	1.18e-2	SMART
ZnF_C2H2	104	126	2.05e-2	SMART
ZnF_C2H2	132	154	2.3e-5	SMART
ZnF_C2H2	160	181	2.45e0	SMART
ZnF_C2H2	187	209	1.92e-2	SMART
ZnF_C2H2	215	237	5.59e-4	SMART
ZnF_C2H2	243	265	4.87e-4	SMART
ZnF_C2H2	271	293	4.94e-5	SMART
ZnF_C2H2	299	321	9.08e-4	SMART
ZnF_C2H2	327	349	1.13e-4	SMART
ZnF_C2H2	355	377	3.16e-3	SMART
ZnF_C2H2	383	405	2.24e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000223163
AA Change: S333G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.4%

Validation Efficiency

97% (66/68)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930486L24Rik	G	A	13: 60,992,948 (GRCm39)	P244S	probably damaging	Het
9130023H24Rik	C	A	7: 127,836,742 (GRCm39)		probably benign	Het
Acoxl	A	T	2: 127,852,085 (GRCm39)	Q129L	probably damaging	Het
Akna	T	C	4: 63,316,268 (GRCm39)	T32A	probably benign	Het
Alox15	C	T	11: 70,235,645 (GRCm39)		probably null	Het
Antxrl	T	C	14: 33,787,864 (GRCm39)	S296P	probably damaging	Het
Arrb2	G	T	11: 70,328,142 (GRCm39)	G52*	probably null	Het
Bltp2	T	C	11: 78,179,105 (GRCm39)	F2091S	probably benign	Het
Capn2	A	T	1: 182,309,045 (GRCm39)	I499N	probably damaging	Het
Cfhr4	T	A	1: 139,629,719 (GRCm39)	D683V	possibly damaging	Het
Cspg4b	T	A	13: 113,504,686 (GRCm39)	D396E	probably benign	Het
Cubn	T	A	2: 13,326,066 (GRCm39)	I2700F	probably damaging	Het
Cypt4	A	T	9: 24,536,965 (GRCm39)	N152Y	probably benign	Het
Dmxl2	T	C	9: 54,316,467 (GRCm39)	N1640D	probably damaging	Het
Dnah7b	A	T	1: 46,379,377 (GRCm39)	N3704Y	possibly damaging	Het
Dnah9	A	G	11: 65,963,565 (GRCm39)		probably null	Het
Elac2	G	A	11: 64,890,265 (GRCm39)		probably null	Het
Erich3	A	T	3: 154,433,029 (GRCm39)		probably null	Het
Espnl	T	C	1: 91,270,351 (GRCm39)	V386A	probably damaging	Het
Fbxl6	T	A	15: 76,422,898 (GRCm39)		probably benign	Het
Fdps	A	C	3: 89,008,068 (GRCm39)	F17V	probably damaging	Het
Gigyf2	T	C	1: 87,346,898 (GRCm39)	I576T	possibly damaging	Het
Gm20939	A	G	17: 95,184,657 (GRCm39)	E435G	possibly damaging	Het
Hivep1	G	A	13: 42,310,852 (GRCm39)	V1031I	probably damaging	Het
Hydin	T	G	8: 111,324,603 (GRCm39)	S4655A	probably benign	Het
Ifrd1	T	A	12: 40,273,157 (GRCm39)		probably benign	Het
Iqgap1	G	T	7: 80,378,729 (GRCm39)	T1219K	possibly damaging	Het
Lmtk3	G	A	7: 45,443,233 (GRCm39)	E639K	possibly damaging	Het
Map3k5	A	G	10: 20,017,326 (GRCm39)	*1373W	probably null	Het
Mia2	G	A	12: 59,235,332 (GRCm39)		probably null	Het
Micu2	T	C	14: 58,156,896 (GRCm39)	D313G	possibly damaging	Het
Mrgpra4	A	G	7: 47,631,371 (GRCm39)	S77P	probably damaging	Het
Mrpl51	T	C	6: 125,169,367 (GRCm39)	V17A	probably benign	Het
Neurod4	A	T	10: 130,106,661 (GRCm39)	Y204*	probably null	Het
Or2n1	A	G	17: 38,486,013 (GRCm39)	I13V	probably benign	Het
Or56a42-ps1	A	T	7: 104,776,217 (GRCm39)	V97E	probably benign	Het
Or5p67	G	A	7: 107,922,460 (GRCm39)	T141I	probably benign	Het
Or6c66b	A	T	10: 129,377,132 (GRCm39)	H242L	probably damaging	Het
Pate2	T	C	9: 35,596,941 (GRCm39)		probably benign	Het
Pcdhgb8	G	A	18: 37,896,580 (GRCm39)	R550Q	probably benign	Het
Phldb3	T	C	7: 24,323,577 (GRCm39)	L437P	possibly damaging	Het
Pianp	C	A	6: 124,978,565 (GRCm39)	P257T	possibly damaging	Het
Rbbp6	T	A	7: 122,584,287 (GRCm39)	H140Q	possibly damaging	Het
Rfx2	A	G	17: 57,087,804 (GRCm39)	I529T	probably damaging	Het
Rnf113a1	A	C	X: 36,455,840 (GRCm39)	T266P	probably benign	Het
Rp1l1	T	C	14: 64,268,599 (GRCm39)	I1395T	possibly damaging	Het
Rps6ka2	A	C	17: 7,519,035 (GRCm39)	K186Q	probably damaging	Het
Rsf1	CGGC	CGGCGGCGGGGGC	7: 97,229,139 (GRCm39)		probably benign	Het
Rtel1	C	A	2: 180,964,256 (GRCm39)	T85N	probably benign	Het
Rtn4rl2	T	C	2: 84,710,967 (GRCm39)	N99S	probably damaging	Het
Ryr1	C	T	7: 28,723,741 (GRCm39)	G4106D	possibly damaging	Het
Scgb2b24	A	G	7: 33,437,894 (GRCm39)	V71A	probably benign	Het
Sgip1	A	G	4: 102,778,225 (GRCm39)		probably benign	Het
Slc12a4	G	A	8: 106,676,371 (GRCm39)	T515I	probably damaging	Het
Spag1	C	T	15: 36,197,895 (GRCm39)	R286*	probably null	Het
Strbp	C	T	2: 37,515,228 (GRCm39)	R266Q	probably damaging	Het
Strn	G	T	17: 78,977,787 (GRCm39)		probably benign	Het
Thnsl2	C	A	6: 71,118,264 (GRCm39)	V55L	probably benign	Het
Tmem178b	A	T	6: 40,231,858 (GRCm39)	Q164L	unknown	Het
Tmprss6	C	T	15: 78,324,457 (GRCm39)	R786H	probably damaging	Het
Ttc21a	T	A	9: 119,770,268 (GRCm39)	L113Q	possibly damaging	Het
Ttc21b	T	C	2: 66,038,994 (GRCm39)		probably null	Het
Vmn2r51	T	C	7: 9,832,191 (GRCm39)	D465G	probably damaging	Het
Vmp1	A	C	11: 86,556,913 (GRCm39)		probably null	Het
Wdcp	T	C	12: 4,901,358 (GRCm39)	F405L	probably damaging	Het
Zfhx3	A	T	8: 109,676,149 (GRCm39)	T2400S	probably benign	Het
Zfp27	T	C	7: 29,593,860 (GRCm39)	T702A	probably benign	Het

Other mutations in Zfp87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02165:Zfp87	APN	13	67,665,989 (GRCm39)	missense	probably damaging	0.99
IGL02372:Zfp87	APN	13	67,668,739 (GRCm39)	splice site	probably benign
IGL03160:Zfp87	APN	13	67,669,392 (GRCm39)	missense	probably damaging	1.00
R0653:Zfp87	UTSW	13	74,520,190 (GRCm39)	missense	probably damaging	1.00
R0711:Zfp87	UTSW	13	74,524,544 (GRCm39)	splice site	probably benign
R1498:Zfp87	UTSW	13	74,520,736 (GRCm39)	missense	probably benign	0.01
R3801:Zfp87	UTSW	13	67,669,334 (GRCm39)	missense	probably damaging	1.00
R4032:Zfp87	UTSW	13	74,520,449 (GRCm39)	missense	possibly damaging	0.62
R4629:Zfp87	UTSW	13	74,520,512 (GRCm39)	missense	probably damaging	1.00
R4950:Zfp87	UTSW	13	67,666,018 (GRCm39)	missense	probably benign	0.20
R5604:Zfp87	UTSW	13	67,665,945 (GRCm39)	missense	probably damaging	1.00
R6111:Zfp87	UTSW	13	74,520,504 (GRCm39)	missense	probably benign	0.31
R6130:Zfp87	UTSW	13	74,520,460 (GRCm39)	missense	possibly damaging	0.75
R6277:Zfp87	UTSW	13	74,520,643 (GRCm39)	nonsense	probably null
R6392:Zfp87	UTSW	13	67,664,986 (GRCm39)	missense	probably benign	0.00
R6909:Zfp87	UTSW	13	74,519,861 (GRCm39)	missense	possibly damaging	0.47
R7009:Zfp87	UTSW	13	67,665,173 (GRCm39)	missense	probably damaging	1.00
R7183:Zfp87	UTSW	13	67,665,593 (GRCm39)	missense	probably damaging	1.00
R7298:Zfp87	UTSW	13	74,520,513 (GRCm39)	missense	possibly damaging	0.93
R7330:Zfp87	UTSW	13	74,523,153 (GRCm39)	missense	probably damaging	0.99
R7341:Zfp87	UTSW	13	74,520,467 (GRCm39)	missense	possibly damaging	0.68
R7448:Zfp87	UTSW	13	67,665,163 (GRCm39)	missense	probably benign	0.01
R7597:Zfp87	UTSW	13	67,665,412 (GRCm39)	missense	probably benign	0.06
R8696:Zfp87	UTSW	13	74,520,599 (GRCm39)	missense	probably damaging	1.00
R9280:Zfp87	UTSW	13	74,520,803 (GRCm39)	missense	probably benign	0.16
R9429:Zfp87	UTSW	13	74,520,703 (GRCm39)	missense	probably damaging	1.00
R9780:Zfp87	UTSW	13	67,665,241 (GRCm39)	missense	probably damaging	1.00
R9782:Zfp87	UTSW	13	74,520,932 (GRCm39)	missense	probably benign	0.00
RF014:Zfp87	UTSW	13	74,523,173 (GRCm39)	missense	probably benign	0.17
Z1176:Zfp87	UTSW	13	67,674,275 (GRCm39)	start gained	probably benign
Z1177:Zfp87	UTSW	13	74,519,911 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCCATCTCTGCATTTATAGGGTTAC -3'
(R):5'- ACAAATGCAGCGAATGTGGC -3'

Sequencing Primer
(F):5'- TTCCCAGAATCTTCACAGCTGTAGAG -3'
(R):5'- TGCAGCGAATGTGGCAAAGC -3'

Posted On

2018-09-12