Mutagenetix > Incidental Mutation

Incidental Mutation 'R6805:Or7g20'

533537

Institutional Source

Beutler Lab

Gene Symbol

Or7g20

Ensembl Gene

ENSMUSG00000045204

Gene Name

olfactory receptor family 7 subfamily G member 20

Synonyms

MOR150-3, GA_x6K02T2PVTD-12771995-12772930, Olfr835

MMRRC Submission

044918-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.127) question?

Stock #

R6805 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

18946421-18947356 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 18946597 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 59 (M59I)

Ref Sequence

ENSEMBL: ENSMUSP00000148416 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059315] [ENSMUST00000213018]

AlphaFold

Q7TRG7

Predicted Effect

probably damaging
Transcript: ENSMUST00000059315
AA Change: M59I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000052610
Gene: ENSMUSG00000045204
AA Change: M59I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.8e-47	PFAM
Pfam:7TM_GPCR_Srsx	35	206	2.9e-7	PFAM
Pfam:7tm_1	41	303	1.3e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213018
AA Change: M59I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.8%
20x: 97.1%

Validation Efficiency

98% (62/63)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930407I10Rik	A	G	15: 81,946,744 (GRCm39)	T214A	possibly damaging	Het
A930009A15Rik	G	T	10: 115,415,810 (GRCm39)		probably benign	Het
Aadac	A	C	3: 59,944,757 (GRCm39)	D143A	probably benign	Het
Acot10	T	G	15: 20,665,452 (GRCm39)	T430P	probably benign	Het
Adgrb3	T	C	1: 25,865,253 (GRCm39)	T197A	possibly damaging	Het
Bbs1	A	G	19: 4,950,643 (GRCm39)	I200T	probably damaging	Het
C1ra	G	A	6: 124,494,684 (GRCm39)	E316K	probably benign	Het
Cadps	G	A	14: 12,467,103 (GRCm38)	A943V	probably damaging	Het
Cc2d2a	A	G	5: 43,838,673 (GRCm39)	E48G	probably damaging	Het
Clca3a1	A	T	3: 144,724,428 (GRCm39)	C211S	probably damaging	Het
Col18a1	A	G	10: 76,890,073 (GRCm39)	L1429P	probably damaging	Het
Cplane1	T	C	15: 8,273,790 (GRCm39)	V2591A	probably benign	Het
Cul2	T	G	18: 3,421,263 (GRCm39)	Y196D	probably damaging	Het
D630023F18Rik	T	C	1: 65,156,365 (GRCm39)	S43G	probably benign	Het
Ddx39a	G	A	8: 84,449,766 (GRCm39)	R427Q	probably damaging	Het
Def6	A	G	17: 28,442,691 (GRCm39)	T285A	probably damaging	Het
Defb21	T	A	2: 152,416,789 (GRCm39)	D88E	probably benign	Het
Defb6	A	G	8: 19,278,117 (GRCm39)	K63R	probably benign	Het
Dnph1	T	C	17: 46,809,670 (GRCm39)	S112P	probably damaging	Het
Dock10	T	A	1: 80,564,407 (GRCm39)	I467L	probably benign	Het
Dspp	C	A	5: 104,323,716 (GRCm39)	H286Q	probably benign	Het
Eya1	T	A	1: 14,253,501 (GRCm39)	T459S	probably benign	Het
Faf1	T	C	4: 109,719,049 (GRCm39)	L385P	probably damaging	Het
Fbxw21	C	A	9: 108,986,633 (GRCm39)	R82L	probably damaging	Het
Fryl	A	G	5: 73,222,437 (GRCm39)	V2048A	probably benign	Het
Galnt5	A	T	2: 57,925,311 (GRCm39)	D864V	possibly damaging	Het
Gata6	T	G	18: 11,054,460 (GRCm39)	S130A	possibly damaging	Het
Gbf1	G	T	19: 46,250,946 (GRCm39)	R434L	probably damaging	Het
Gga3	A	G	11: 115,476,588 (GRCm39)	F709L	probably damaging	Het
Hcar1	A	G	5: 124,017,193 (GRCm39)	V166A	probably benign	Het
Hexa	T	A	9: 59,471,220 (GRCm39)	N491K	possibly damaging	Het
Hpse2	A	T	19: 43,282,760 (GRCm39)	C164*	probably null	Het
Ifi202b	T	C	1: 173,802,555 (GRCm39)	Y93C	probably damaging	Het
Iftap	T	C	2: 101,396,804 (GRCm39)	K192E	probably benign	Het
Iscu	T	A	5: 113,913,304 (GRCm39)	I79N	probably damaging	Het
Jmjd7	T	A	2: 119,861,804 (GRCm39)	Y182*	probably null	Het
Jup	A	T	11: 100,274,284 (GRCm39)	D135E	probably benign	Het
Kit	T	A	5: 75,813,468 (GRCm39)	I881N	probably damaging	Het
Llgl1	T	A	11: 60,593,691 (GRCm39)	S55T	probably benign	Het
Lonp2	G	A	8: 87,435,724 (GRCm39)	M653I	probably benign	Het
Lrp8	T	C	4: 107,711,517 (GRCm39)	Y307H	probably damaging	Het
Med13	A	T	11: 86,169,622 (GRCm39)	M1914K	possibly damaging	Het
Ms4a1	A	G	19: 11,230,537 (GRCm39)		probably null	Het
Naip1	A	G	13: 100,563,849 (GRCm39)	S439P	probably benign	Het
Nrg1	T	C	8: 32,311,292 (GRCm39)	R476G	probably damaging	Het
Or10ag59	T	A	2: 87,405,262 (GRCm39)		probably null	Het
Pds5b	T	A	5: 150,729,026 (GRCm39)		probably null	Het
Phf12	G	A	11: 77,918,199 (GRCm39)	G804R	probably damaging	Het
Pou6f2	T	C	13: 18,414,074 (GRCm39)	T234A		Het
Prune2	A	T	19: 17,097,954 (GRCm39)	I1153L	probably benign	Het
Ptprc	C	T	1: 137,995,623 (GRCm39)		probably null	Het
Qpctl	T	C	7: 18,883,079 (GRCm39)	Q11R	probably benign	Het
Rfx4	A	G	10: 84,676,092 (GRCm39)	K103E	possibly damaging	Het
Srcin1	T	A	11: 97,442,806 (GRCm39)		probably null	Het
St6galnac1	G	T	11: 116,659,770 (GRCm39)	A181D	probably damaging	Het
Stk36	T	C	1: 74,661,398 (GRCm39)	V475A	probably benign	Het
Tbc1d21	T	C	9: 58,268,571 (GRCm39)	T263A	possibly damaging	Het
Tex24	A	T	8: 27,835,028 (GRCm39)	K185N	probably damaging	Het
Tnxb	T	A	17: 34,917,127 (GRCm39)	V2174E	possibly damaging	Het
Tollip	A	G	7: 141,444,582 (GRCm39)	S57P	probably benign	Het
Zbtb49	A	G	5: 38,370,585 (GRCm39)		probably benign	Het
Zfp758	A	G	17: 22,580,650 (GRCm39)	T30A	probably benign	Het

Other mutations in Or7g20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01718:Or7g20	APN	9	18,946,584 (GRCm39)	nonsense	probably null
IGL02546:Or7g20	APN	9	18,946,650 (GRCm39)	missense	possibly damaging	0.88
R0010:Or7g20	UTSW	9	18,946,618 (GRCm39)	missense	probably damaging	1.00
R0107:Or7g20	UTSW	9	18,946,629 (GRCm39)	missense	probably damaging	1.00
R1867:Or7g20	UTSW	9	18,946,562 (GRCm39)	missense	probably benign	0.36
R1891:Or7g20	UTSW	9	18,947,274 (GRCm39)	missense	probably damaging	0.99
R1941:Or7g20	UTSW	9	18,947,162 (GRCm39)	missense	possibly damaging	0.93
R4152:Or7g20	UTSW	9	18,946,816 (GRCm39)	nonsense	probably null
R5451:Or7g20	UTSW	9	18,946,787 (GRCm39)	missense	probably damaging	1.00
R5677:Or7g20	UTSW	9	18,946,854 (GRCm39)	missense	possibly damaging	0.93
R5970:Or7g20	UTSW	9	18,946,443 (GRCm39)	missense	probably benign
R6187:Or7g20	UTSW	9	18,946,689 (GRCm39)	missense	probably benign	0.12
R7183:Or7g20	UTSW	9	18,946,628 (GRCm39)	missense	probably damaging	1.00
R8029:Or7g20	UTSW	9	18,947,090 (GRCm39)	missense	probably damaging	1.00
R8139:Or7g20	UTSW	9	18,946,871 (GRCm39)	missense	probably benign	0.07
R8736:Or7g20	UTSW	9	18,946,774 (GRCm39)	missense	probably damaging	1.00
R8868:Or7g20	UTSW	9	18,946,778 (GRCm39)	missense	probably damaging	1.00
R8909:Or7g20	UTSW	9	18,946,888 (GRCm39)	missense	probably benign	0.08
R9116:Or7g20	UTSW	9	18,946,773 (GRCm39)	missense	probably damaging	1.00
R9479:Or7g20	UTSW	9	18,946,730 (GRCm39)	missense	probably benign	0.30
R9786:Or7g20	UTSW	9	18,947,241 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GGCCTTTTAGAATATAGGCTACTGTC -3'
(R):5'- GGCCATTGCTGCAAGAAGAC -3'

Sequencing Primer
(F):5'- TTGTCAAAGACATGGGAGGTAAAAAC -3'
(R):5'- TTGCTGCAAGAAGACAGCTTTCG -3'

Posted On

2018-09-12