Mutagenetix > Incidental Mutation

Incidental Mutation 'R6810:Vmn2r99'

533781

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R6810 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19380034 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 440 (K440R)

Ref Sequence

ENSEMBL: ENSMUSP00000156067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably benign
Transcript: ENSMUST00000176107
AA Change: K440R

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: K440R

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231989
AA Change: K440R

PolyPhen 2 Score 0.199 (Sensitivity: 0.92; Specificity: 0.88)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 98.8%
20x: 96.7%

Validation Efficiency

96% (53/55)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930444G20Rik	C	T	10: 22,066,717	G455R	probably damaging	Het
Adgrg5	A	T	8: 94,933,942	T70S	probably damaging	Het
Adora1	T	C	1: 134,234,039	Y106C	probably damaging	Het
Aox3	T	A	1: 58,141,431	N250K	probably benign	Het
Ap2b1	A	T	11: 83,335,491	Y238F	possibly damaging	Het
Birc6	G	A	17: 74,612,220	S2015N	possibly damaging	Het
C7	A	G	15: 5,007,654	F581L	probably damaging	Het
Cd27	A	T	6: 125,233,664	H203Q	probably damaging	Het
Cdk2	A	G	10: 128,699,587	V274A	probably benign	Het
Cenpe	A	G	3: 135,243,822	T1351A	probably benign	Het
Chd7	T	C	4: 8,839,523	L1353P	probably damaging	Het
Dcc	C	T	18: 71,370,693	V945M	probably damaging	Het
Dio1	A	G	4: 107,297,725	V118A	probably damaging	Het
Dst	C	T	1: 34,212,298	T1818M	probably damaging	Het
Dthd1	T	A	5: 62,814,329	M165K	probably benign	Het
Eif5b	A	G	1: 38,046,660	I929V	probably benign	Het
F5	T	A	1: 164,186,902	S581T	probably damaging	Het
Fanca	A	C	8: 123,286,477	I761S	probably damaging	Het
Fat2	T	C	11: 55,282,241	T2549A	possibly damaging	Het
Fut2	A	G	7: 45,650,505	L281P	probably damaging	Het
Gm19410	C	A	8: 35,772,579	A143E	probably damaging	Het
Gm5431	T	C	11: 48,888,976	D651G	probably damaging	Het
Hook3	A	C	8: 26,032,422		probably null	Het
Ivd	G	T	2: 118,869,761	V90L	probably benign	Het
Klhdc7b	A	G	15: 89,388,356	Y1147C	possibly damaging	Het
Mlh1	G	A	9: 111,241,558	T363M	possibly damaging	Het
Ndufa3	A	T	7: 3,619,477	I45F	probably damaging	Het
Nell2	T	C	15: 95,241,587	D588G	probably damaging	Het
Nhlrc3	T	C	3: 53,453,575	N253S	probably benign	Het
Nlrp4c	T	A	7: 6,066,755	F552I	probably damaging	Het
Olfr1052	G	A	2: 86,297,923	A36T	probably benign	Het
Olfr578	A	C	7: 102,984,835	S110A	probably damaging	Het
Pcdhga12	T	C	18: 37,767,179	S355P	probably benign	Het
Pcdhga7	A	T	18: 37,715,873	Y311F	probably benign	Het
Phldb2	A	G	16: 45,748,725		probably null	Het
Plscr4	G	A	9: 92,483,836	V120I	probably damaging	Het
Plxna4	A	G	6: 32,310,522	V480A	probably benign	Het
Psrc1	A	T	3: 108,385,348	K152N	possibly damaging	Het
Ptcd3	A	C	6: 71,885,532	V473G	probably damaging	Het
Rab11fip1	ACTCT	ACT	8: 27,152,732		probably null	Het
Skint6	T	C	4: 112,948,380		probably null	Het
Slc24a1	A	G	9: 64,948,323	V434A	probably benign	Het
Snd1	T	A	6: 28,668,610	V432E	probably benign	Het
Syne2	C	T	12: 75,942,885	T1847M	probably benign	Het
Tenm4	G	A	7: 96,553,496	R106H	probably benign	Het
Tes	C	A	6: 17,104,652	N377K	probably benign	Het
Tfip11	T	A	5: 112,333,597	I452N	probably benign	Het
Tgfbi	T	C	13: 56,637,203	S658P	probably benign	Het
Tmx4	A	C	2: 134,620,674	D112E	probably damaging	Het
Tnn	G	T	1: 160,104,842	D1367E	probably damaging	Het
Triobp	A	G	15: 78,966,615	N323S	possibly damaging	Het
Usp40	T	C	1: 87,981,033	D582G	probably benign	Het
Zfp707	T	A	15: 75,974,899	L193Q	probably damaging	Het
Zfp748	T	C	13: 67,541,725	Y472C	probably damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19378854	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19394256	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19382623	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19393658	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19380115	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19380232	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19378690	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19378223	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19394285	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19394285	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19394343	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19394573	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19379043	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19362259	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19380060	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19362252	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19377945	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19362153	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19378815	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19377991	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19378629	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19378708	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19394373	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19378990	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19378990	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19378570	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19379260	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19393662	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19362135	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19378606	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19379339	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19379269	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19394146	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19377948	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19378980	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19382558	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19382558	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19382605	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19380031	missense	probably damaging	1.00
R6885:Vmn2r99	UTSW	17	19380195	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19378110	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19394564	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19393710	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19379311	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19379145	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19393817	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19380040	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19393758	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19394181	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19393660	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19378126	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19378627	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19362301	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19379301	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- AGCAAAGGAGTCTTGTTAGGTTTC -3'
(R):5'- GGCCATTGGATCATCTGTTCAG -3'

Sequencing Primer
(F):5'- AGATCAGTTGTCATTGAGTGTTTAC -3'
(R):5'- GATAAACACAACTGCTGATCCTGGG -3'

Posted On

2018-09-12