Mutagenetix > Incidental Mutation

Incidental Mutation 'R6811:Vmn2r60'

533803

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Casr-rs3, EG637898, Gprc2a-rs3

MMRRC Submission

045018-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R6811 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41765895-41845200 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 41844310 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 558 (C558S)

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166447
AA Change: C558S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619
AA Change: C558S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 99.1%
20x: 97.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrb1	T	C	15: 74,401,210 (GRCm39)	S69P	probably damaging	Het
Alkbh7	G	T	17: 57,304,392 (GRCm39)	R10L	probably benign	Het
Ano3	T	C	2: 110,711,212 (GRCm39)	E84G	probably benign	Het
Asxl3	A	G	18: 22,655,968 (GRCm39)	E1326G	possibly damaging	Het
Atrnl1	T	G	19: 57,643,393 (GRCm39)	M427R	probably damaging	Het
Cenpf	T	C	1: 189,386,739 (GRCm39)	E1847G	probably benign	Het
Csf1r	T	C	18: 61,252,125 (GRCm39)	Y536H	probably damaging	Het
Dnm3	T	C	1: 162,148,652 (GRCm39)	K240E	probably damaging	Het
Dsc1	T	G	18: 20,222,711 (GRCm39)	E587A	probably benign	Het
Gm19410	C	A	8: 36,239,733 (GRCm39)	A143E	probably damaging	Het
Gm19965	G	T	1: 116,731,809 (GRCm39)	L38F	probably damaging	Het
Helz	T	C	11: 107,510,144 (GRCm39)		probably null	Het
Herc2	C	T	7: 55,763,181 (GRCm39)	R929*	probably null	Het
Iqcn	T	C	8: 71,169,422 (GRCm39)	S1171P	probably benign	Het
Krt31	A	G	11: 99,939,242 (GRCm39)	L225P	probably damaging	Het
Lrp1b	T	C	2: 40,605,512 (GRCm39)		probably null	Het
Lrp1b	A	G	2: 41,339,206 (GRCm39)	V765A	probably benign	Het
Lsm5	A	G	6: 56,679,127 (GRCm39)	I34T	possibly damaging	Het
Ly6g6c	T	C	17: 35,288,386 (GRCm39)	L86P	probably damaging	Het
Meak7	A	T	8: 120,495,029 (GRCm39)	I243N	possibly damaging	Het
Megf11	A	G	9: 64,451,923 (GRCm39)	T116A	probably damaging	Het
Megf6	T	C	4: 154,336,618 (GRCm39)	C190R	probably damaging	Het
Mroh9	A	G	1: 162,873,610 (GRCm39)	V515A	possibly damaging	Het
Mtbp	C	A	15: 55,469,942 (GRCm39)		probably null	Het
Myo9b	T	A	8: 71,809,222 (GRCm39)	F1810L	probably damaging	Het
Nacad	T	G	11: 6,549,400 (GRCm39)	K180Q	possibly damaging	Het
Ncf2	G	A	1: 152,711,791 (GRCm39)	V502I	probably benign	Het
Npsr1	T	A	9: 24,046,105 (GRCm39)	C75S	probably benign	Het
Oog3	T	C	4: 143,886,152 (GRCm39)	T149A	probably benign	Het
Pank1	T	A	19: 34,818,422 (GRCm39)	Q39L	probably benign	Het
Pdx1	T	C	5: 147,211,474 (GRCm39)	S232P	possibly damaging	Het
Peg10	T	TCCA	6: 4,756,451 (GRCm39)		probably benign	Het
Pirb	A	T	7: 3,722,641 (GRCm39)	V117E	possibly damaging	Het
Ppl	G	A	16: 4,907,008 (GRCm39)	L1096F	probably damaging	Het
Rev3l	T	A	10: 39,706,917 (GRCm39)	Y2223*	probably null	Het
Slc16a4	A	G	3: 107,206,233 (GRCm39)	Y101C	probably benign	Het
Slc17a3	A	T	13: 24,039,924 (GRCm39)	I321F	possibly damaging	Het
Sufu	T	A	19: 46,438,317 (GRCm39)	D168E	probably damaging	Het
Tenm4	G	A	7: 96,202,703 (GRCm39)	R106H	probably benign	Het
Vmn1r237	T	G	17: 21,534,648 (GRCm39)	S124A	probably benign	Het
Vwa5b1	G	A	4: 138,319,414 (GRCm39)	T414I	probably benign	Het
Zbtb47	T	C	9: 121,595,595 (GRCm39)	S573P	probably damaging	Het
Zfp971	T	G	2: 177,675,674 (GRCm39)	C424W	possibly damaging	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	41,844,578 (GRCm39)	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	41,844,890 (GRCm39)	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	41,845,010 (GRCm39)	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	41,791,720 (GRCm39)	nonsense	probably null
IGL03135:Vmn2r60	APN	7	41,786,018 (GRCm39)	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	41,765,971 (GRCm39)	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	41,844,564 (GRCm39)	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	41,784,985 (GRCm39)	splice site	probably benign
R0328:Vmn2r60	UTSW	7	41,791,744 (GRCm39)	splice site	probably benign
R0464:Vmn2r60	UTSW	7	41,785,255 (GRCm39)	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	41,844,869 (GRCm39)	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	41,844,365 (GRCm39)	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	41,845,195 (GRCm39)	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	41,786,476 (GRCm39)	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	41,786,137 (GRCm39)	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	41,785,279 (GRCm39)	nonsense	probably null
R1628:Vmn2r60	UTSW	7	41,785,830 (GRCm39)	nonsense	probably null
R1883:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	41,844,931 (GRCm39)	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	41,786,251 (GRCm39)	nonsense	probably null
R2847:Vmn2r60	UTSW	7	41,785,857 (GRCm39)	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	41,790,403 (GRCm39)	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	41,785,220 (GRCm39)	missense	probably benign
R2921:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	41,765,980 (GRCm39)	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	41,785,878 (GRCm39)	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	41,844,667 (GRCm39)	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	41,785,285 (GRCm39)	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	41,786,448 (GRCm39)	missense	possibly damaging	0.93
R5480:Vmn2r60	UTSW	7	41,785,154 (GRCm39)	missense	probably damaging	0.98
R5521:Vmn2r60	UTSW	7	41,845,049 (GRCm39)	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	41,765,932 (GRCm39)	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	41,844,847 (GRCm39)	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	41,786,400 (GRCm39)	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	41,785,045 (GRCm39)	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	41,765,895 (GRCm39)	start codon destroyed	probably null	0.04
R6876:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	41,791,716 (GRCm39)	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	41,791,666 (GRCm39)	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	41,786,487 (GRCm39)	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	41,844,536 (GRCm39)	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	41,786,166 (GRCm39)	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	41,785,826 (GRCm39)	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	41,844,631 (GRCm39)	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7527:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7528:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7764:Vmn2r60	UTSW	7	41,844,535 (GRCm39)	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	41,844,511 (GRCm39)	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	41,790,521 (GRCm39)	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	41,791,690 (GRCm39)	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	41,790,494 (GRCm39)	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	41,844,954 (GRCm39)	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	41,844,850 (GRCm39)	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	41,786,169 (GRCm39)	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	41,791,654 (GRCm39)	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	41,790,518 (GRCm39)	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	41,785,778 (GRCm39)	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	41,791,723 (GRCm39)	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	41,785,955 (GRCm39)	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	41,844,332 (GRCm39)	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	41,785,172 (GRCm39)	missense	probably benign
RF024:Vmn2r60	UTSW	7	41,790,363 (GRCm39)	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	41,790,538 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCCACTATAAGCACTGTATAGACTTG -3'
(R):5'- AGGATGTAGCTGAGAGTCCTG -3'

Sequencing Primer
(F):5'- AGCACTGTATAGACTTGAAATGATTC -3'
(R):5'- CCTGTTATTGGCCTTGACAATG -3'

Posted On

2018-09-12