Mutagenetix > Incidental Mutation

Incidental Mutation 'R6842:Zfp956'

533902

Institutional Source

Beutler Lab

Gene Symbol

Zfp956

Ensembl Gene

ENSMUSG00000045466

Gene Name

zinc finger protein 956

Synonyms

AI894139

MMRRC Submission

044948-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.100) question?

Stock #

R6842 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

47930324-47942234 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 47940763 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 374 (T374I)

Ref Sequence

ENSEMBL: ENSMUSP00000098989 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000101445] [ENSMUST00000140719]

AlphaFold

Q3UZY6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000101445
AA Change: T374I

PolyPhen 2 Score 0.469 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000098989
Gene: ENSMUSG00000045466
AA Change: T374I

Domain	Start	End	E-Value	Type
Pfam:DUF3669	39	99	2.1e-9	PFAM
KRAB	129	191	2.4e-1	SMART
ZnF_C2H2	325	347	2.24e-3	SMART
ZnF_C2H2	353	375	2.09e-3	SMART
ZnF_C2H2	381	403	6.78e-3	SMART
ZnF_C2H2	409	431	2.57e-3	SMART
ZnF_C2H2	437	459	3.16e-3	SMART
ZnF_C2H2	465	487	3.58e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000140719

SMART Domains

Protein: ENSMUSP00000114227
Gene: ENSMUSG00000045466

Domain	Start	End	E-Value	Type
Pfam:DUF3669	22	101	2.4e-16	PFAM
Blast:KRAB	129	179	2e-20	BLAST

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsbg3	A	G	17: 57,184,432 (GRCm39)	N69S	probably benign	Het
Adgrl3	C	T	5: 81,888,927 (GRCm39)	A1042V	probably damaging	Het
Armh3	A	G	19: 45,807,416 (GRCm39)	V660A	probably benign	Het
C1s2	T	A	6: 124,604,461 (GRCm39)	H395L	probably benign	Het
Cacna1e	A	G	1: 154,358,863 (GRCm39)	I307T	probably damaging	Het
Cap2	T	C	13: 46,800,101 (GRCm39)	S381P	probably damaging	Het
Cbfa2t2	A	G	2: 154,365,965 (GRCm39)	T392A	probably benign	Het
Ccdc127	T	C	13: 74,505,088 (GRCm39)	I212T	probably damaging	Het
Cela3a	A	G	4: 137,132,979 (GRCm39)	V91A	probably benign	Het
Cep85	C	G	4: 133,883,167 (GRCm39)	A241P	probably benign	Het
Csmd2	T	C	4: 128,402,952 (GRCm39)	F2347L	possibly damaging	Het
Ddx19a	A	G	8: 111,705,257 (GRCm39)	V288A	possibly damaging	Het
Fbxl5	T	C	5: 43,930,928 (GRCm39)	E53G	probably damaging	Het
Fbxw26	A	T	9: 109,553,988 (GRCm39)	I217N	probably damaging	Het
Fgfr1op2	T	A	6: 146,491,536 (GRCm39)		probably null	Het
Ighv1-37	A	T	12: 114,860,275 (GRCm39)	I6N	probably damaging	Het
Klhdc10	T	C	6: 30,439,781 (GRCm39)	L128P	probably damaging	Het
Lypla1	C	T	1: 4,902,563 (GRCm39)	S24F	probably benign	Het
Mme	T	A	3: 63,269,465 (GRCm39)	D591E	probably damaging	Het
Mmp1b	T	A	9: 7,384,888 (GRCm39)	I254F	probably damaging	Het
Msr1	T	C	8: 40,085,866 (GRCm39)	M5V	probably benign	Het
Myh8	A	G	11: 67,175,481 (GRCm39)	D312G	probably damaging	Het
Nav2	T	C	7: 49,107,917 (GRCm39)	Y709H	possibly damaging	Het
Oas1g	T	C	5: 121,025,621 (GRCm39)	E2G	probably benign	Het
Ocm	T	A	5: 143,962,509 (GRCm39)	I6F	unknown	Het
Odad2	C	T	18: 7,268,401 (GRCm39)	D373N	probably benign	Het
Or13f5	T	C	4: 52,825,576 (GRCm39)	Y60H	probably damaging	Het
Or2ag12	T	C	7: 106,277,093 (GRCm39)	Y200C	probably damaging	Het
Or8a1b	T	C	9: 37,622,885 (GRCm39)	N230S	probably benign	Het
Or8b12i	A	G	9: 20,082,549 (GRCm39)	L106P	possibly damaging	Het
Pax1	A	G	2: 147,215,640 (GRCm39)	D419G	probably benign	Het
Plbd1	T	A	6: 136,612,612 (GRCm39)	I194F	probably benign	Het
Prdx6	A	T	1: 161,074,940 (GRCm39)	C47S	probably damaging	Het
Sec24d	T	C	3: 123,136,868 (GRCm39)	S534P	probably benign	Het
Sgk3	T	C	1: 9,968,979 (GRCm39)	V452A	probably benign	Het
Sipa1l1	T	C	12: 82,467,320 (GRCm39)	V1177A	probably benign	Het
Tgfbr1	T	C	4: 47,383,757 (GRCm39)	C32R	probably damaging	Het
Trim66	T	C	7: 109,059,983 (GRCm39)	N801S	probably benign	Het
Utp6	C	T	11: 79,831,775 (GRCm39)	S504N	probably benign	Het
Wdsub1	A	C	2: 59,708,532 (GRCm39)	S114A	probably benign	Het
Wfikkn2	T	C	11: 94,128,866 (GRCm39)	E425G	probably damaging	Het

Other mutations in Zfp956

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01110:Zfp956	APN	6	47,940,346 (GRCm39)	missense	probably benign	0.28
ANU74:Zfp956	UTSW	6	47,940,507 (GRCm39)	missense	probably benign	0.00
R0603:Zfp956	UTSW	6	47,932,962 (GRCm39)	missense	probably damaging	1.00
R1243:Zfp956	UTSW	6	47,932,985 (GRCm39)	missense	probably damaging	0.98
R1879:Zfp956	UTSW	6	47,940,678 (GRCm39)	missense	probably benign	0.19
R1999:Zfp956	UTSW	6	47,940,805 (GRCm39)	missense	probably damaging	1.00
R2106:Zfp956	UTSW	6	47,941,359 (GRCm39)	makesense	probably null
R2150:Zfp956	UTSW	6	47,940,805 (GRCm39)	missense	probably damaging	1.00
R4737:Zfp956	UTSW	6	47,939,476 (GRCm39)	missense	probably damaging	0.99
R4740:Zfp956	UTSW	6	47,939,476 (GRCm39)	missense	probably damaging	0.99
R4751:Zfp956	UTSW	6	47,940,510 (GRCm39)	missense	probably benign	0.00
R4832:Zfp956	UTSW	6	47,928,987 (GRCm39)	unclassified	probably benign
R5325:Zfp956	UTSW	6	47,928,012 (GRCm39)	unclassified	probably benign
R5523:Zfp956	UTSW	6	47,930,455 (GRCm39)	start gained	probably benign
R7131:Zfp956	UTSW	6	47,932,781 (GRCm39)	missense	probably benign	0.00
R7367:Zfp956	UTSW	6	47,940,853 (GRCm39)	missense	probably damaging	1.00
R8806:Zfp956	UTSW	6	47,933,042 (GRCm39)	missense	probably benign	0.00
R9074:Zfp956	UTSW	6	47,939,462 (GRCm39)	missense	possibly damaging	0.67
R9248:Zfp956	UTSW	6	47,934,437 (GRCm39)	missense	possibly damaging	0.92
R9452:Zfp956	UTSW	6	47,940,370 (GRCm39)	missense	probably benign
R9464:Zfp956	UTSW	6	47,941,041 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCACTCTCACCAGGAATC -3'
(R):5'- GTGAAGCATGAGCCACACTG -3'

Sequencing Primer
(F):5'- TCTCACCAGGAATCGCCGTG -3'
(R):5'- CACACTGGGTGCACTGGAAG -3'

Posted On

2018-09-12