Incidental Mutation 'R6842:Trim66'
ID 533908
Institutional Source Beutler Lab
Gene Symbol Trim66
Ensembl Gene ENSMUSG00000031026
Gene Name tripartite motif-containing 66
Synonyms Tif1d, D7H11orf29
MMRRC Submission 044948-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.169) question?
Stock # R6842 (G1)
Quality Score 225.009
Status Not validated
Chromosome 7
Chromosomal Location 109048213-109107341 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 109059983 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Asparagine to Serine at position 801 (N801S)
Ref Sequence ENSEMBL: ENSMUSP00000102352 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033339] [ENSMUST00000106739] [ENSMUST00000106741]
AlphaFold Q924W6
Predicted Effect probably benign
Transcript: ENSMUST00000033339
AA Change: N699S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000033339
Gene: ENSMUSG00000031026
AA Change: N699S

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000106739
AA Change: N699S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000102350
Gene: ENSMUSG00000031026
AA Change: N699S

DomainStartEndE-ValueType
PHD 4 69 7.77e0 SMART
BBC 108 234 1.61e-39 SMART
low complexity region 318 333 N/A INTRINSIC
low complexity region 452 486 N/A INTRINSIC
low complexity region 517 530 N/A INTRINSIC
low complexity region 568 581 N/A INTRINSIC
PHD 998 1041 4.09e-10 SMART
BROMO 1069 1175 8.22e-27 SMART
low complexity region 1185 1199 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000106741
AA Change: N801S

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000102352
Gene: ENSMUSG00000031026
AA Change: N801S

DomainStartEndE-ValueType
RING 28 78 2.38e-2 SMART
BBOX 102 140 1.48e0 SMART
PHD 106 171 7.77e0 SMART
RING 107 170 4.38e0 SMART
BBOX 162 203 4.21e-3 SMART
BBC 210 336 1.61e-39 SMART
low complexity region 420 435 N/A INTRINSIC
low complexity region 554 588 N/A INTRINSIC
low complexity region 619 632 N/A INTRINSIC
low complexity region 670 683 N/A INTRINSIC
PHD 1100 1143 4.09e-10 SMART
BROMO 1171 1277 8.22e-27 SMART
low complexity region 1287 1301 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.5%
  • 20x: 98.2%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsbg3 A G 17: 57,184,432 (GRCm39) N69S probably benign Het
Adgrl3 C T 5: 81,888,927 (GRCm39) A1042V probably damaging Het
Armh3 A G 19: 45,807,416 (GRCm39) V660A probably benign Het
C1s2 T A 6: 124,604,461 (GRCm39) H395L probably benign Het
Cacna1e A G 1: 154,358,863 (GRCm39) I307T probably damaging Het
Cap2 T C 13: 46,800,101 (GRCm39) S381P probably damaging Het
Cbfa2t2 A G 2: 154,365,965 (GRCm39) T392A probably benign Het
Ccdc127 T C 13: 74,505,088 (GRCm39) I212T probably damaging Het
Cela3a A G 4: 137,132,979 (GRCm39) V91A probably benign Het
Cep85 C G 4: 133,883,167 (GRCm39) A241P probably benign Het
Csmd2 T C 4: 128,402,952 (GRCm39) F2347L possibly damaging Het
Ddx19a A G 8: 111,705,257 (GRCm39) V288A possibly damaging Het
Fbxl5 T C 5: 43,930,928 (GRCm39) E53G probably damaging Het
Fbxw26 A T 9: 109,553,988 (GRCm39) I217N probably damaging Het
Fgfr1op2 T A 6: 146,491,536 (GRCm39) probably null Het
Ighv1-37 A T 12: 114,860,275 (GRCm39) I6N probably damaging Het
Klhdc10 T C 6: 30,439,781 (GRCm39) L128P probably damaging Het
Lypla1 C T 1: 4,902,563 (GRCm39) S24F probably benign Het
Mme T A 3: 63,269,465 (GRCm39) D591E probably damaging Het
Mmp1b T A 9: 7,384,888 (GRCm39) I254F probably damaging Het
Msr1 T C 8: 40,085,866 (GRCm39) M5V probably benign Het
Myh8 A G 11: 67,175,481 (GRCm39) D312G probably damaging Het
Nav2 T C 7: 49,107,917 (GRCm39) Y709H possibly damaging Het
Oas1g T C 5: 121,025,621 (GRCm39) E2G probably benign Het
Ocm T A 5: 143,962,509 (GRCm39) I6F unknown Het
Odad2 C T 18: 7,268,401 (GRCm39) D373N probably benign Het
Or13f5 T C 4: 52,825,576 (GRCm39) Y60H probably damaging Het
Or2ag12 T C 7: 106,277,093 (GRCm39) Y200C probably damaging Het
Or8a1b T C 9: 37,622,885 (GRCm39) N230S probably benign Het
Or8b12i A G 9: 20,082,549 (GRCm39) L106P possibly damaging Het
Pax1 A G 2: 147,215,640 (GRCm39) D419G probably benign Het
Plbd1 T A 6: 136,612,612 (GRCm39) I194F probably benign Het
Prdx6 A T 1: 161,074,940 (GRCm39) C47S probably damaging Het
Sec24d T C 3: 123,136,868 (GRCm39) S534P probably benign Het
Sgk3 T C 1: 9,968,979 (GRCm39) V452A probably benign Het
Sipa1l1 T C 12: 82,467,320 (GRCm39) V1177A probably benign Het
Tgfbr1 T C 4: 47,383,757 (GRCm39) C32R probably damaging Het
Utp6 C T 11: 79,831,775 (GRCm39) S504N probably benign Het
Wdsub1 A C 2: 59,708,532 (GRCm39) S114A probably benign Het
Wfikkn2 T C 11: 94,128,866 (GRCm39) E425G probably damaging Het
Zfp956 C T 6: 47,940,763 (GRCm39) T374I possibly damaging Het
Other mutations in Trim66
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01539:Trim66 APN 7 109,054,273 (GRCm39) missense probably benign 0.02
IGL01758:Trim66 APN 7 109,085,252 (GRCm39) critical splice donor site probably null
IGL01982:Trim66 APN 7 109,057,970 (GRCm39) missense probably benign 0.00
IGL01983:Trim66 APN 7 109,057,458 (GRCm39) nonsense probably null
IGL02149:Trim66 APN 7 109,060,109 (GRCm39) missense possibly damaging 0.66
IGL02392:Trim66 APN 7 109,059,481 (GRCm39) missense probably benign 0.01
IGL02483:Trim66 APN 7 109,076,837 (GRCm39) splice site probably benign
IGL02832:Trim66 APN 7 109,059,704 (GRCm39) missense probably damaging 1.00
IGL02945:Trim66 APN 7 109,059,383 (GRCm39) nonsense probably null
IGL03085:Trim66 APN 7 109,057,952 (GRCm39) missense probably benign 0.17
PIT1430001:Trim66 UTSW 7 109,074,454 (GRCm39) missense probably damaging 0.99
R0326:Trim66 UTSW 7 109,059,379 (GRCm39) missense probably benign 0.00
R0358:Trim66 UTSW 7 109,059,383 (GRCm39) nonsense probably null
R0401:Trim66 UTSW 7 109,074,471 (GRCm39) missense probably damaging 0.98
R0470:Trim66 UTSW 7 109,056,749 (GRCm39) splice site probably benign
R0568:Trim66 UTSW 7 109,059,902 (GRCm39) missense probably benign 0.00
R0669:Trim66 UTSW 7 109,054,199 (GRCm39) intron probably benign
R0980:Trim66 UTSW 7 109,054,877 (GRCm39) missense probably damaging 1.00
R1015:Trim66 UTSW 7 109,054,440 (GRCm39) missense probably damaging 1.00
R1078:Trim66 UTSW 7 109,071,526 (GRCm39) missense probably damaging 1.00
R1099:Trim66 UTSW 7 109,074,661 (GRCm39) missense probably benign 0.34
R1181:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R1497:Trim66 UTSW 7 109,083,826 (GRCm39) missense probably benign 0.00
R1583:Trim66 UTSW 7 109,054,287 (GRCm39) missense probably damaging 1.00
R1843:Trim66 UTSW 7 109,075,046 (GRCm39) missense probably damaging 0.99
R1998:Trim66 UTSW 7 109,083,784 (GRCm39) critical splice donor site probably null
R2016:Trim66 UTSW 7 109,071,439 (GRCm39) critical splice donor site probably null
R2143:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2144:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R2145:Trim66 UTSW 7 109,074,320 (GRCm39) missense probably damaging 0.98
R3945:Trim66 UTSW 7 109,071,475 (GRCm39) missense possibly damaging 0.94
R4012:Trim66 UTSW 7 109,057,338 (GRCm39) missense probably damaging 0.98
R4464:Trim66 UTSW 7 109,076,897 (GRCm39) missense possibly damaging 0.51
R4473:Trim66 UTSW 7 109,081,202 (GRCm39) missense probably damaging 1.00
R4729:Trim66 UTSW 7 109,055,267 (GRCm39) critical splice donor site probably null
R4730:Trim66 UTSW 7 109,082,276 (GRCm39) missense probably damaging 1.00
R4775:Trim66 UTSW 7 109,056,796 (GRCm39) nonsense probably null
R4819:Trim66 UTSW 7 109,056,793 (GRCm39) missense probably damaging 1.00
R5269:Trim66 UTSW 7 109,056,797 (GRCm39) missense probably benign 0.00
R5557:Trim66 UTSW 7 109,082,944 (GRCm39) missense probably benign 0.06
R5832:Trim66 UTSW 7 109,054,409 (GRCm39) missense probably damaging 1.00
R6220:Trim66 UTSW 7 109,082,300 (GRCm39) missense probably damaging 0.97
R6243:Trim66 UTSW 7 109,059,481 (GRCm39) missense probably benign 0.01
R6374:Trim66 UTSW 7 109,085,269 (GRCm39) missense probably benign
R6450:Trim66 UTSW 7 109,059,945 (GRCm39) missense probably benign 0.09
R6543:Trim66 UTSW 7 109,075,086 (GRCm39) missense probably benign 0.01
R6788:Trim66 UTSW 7 109,076,961 (GRCm39) missense probably damaging 1.00
R7169:Trim66 UTSW 7 109,054,328 (GRCm39) missense probably benign 0.25
R7257:Trim66 UTSW 7 109,059,451 (GRCm39) missense probably damaging 1.00
R7328:Trim66 UTSW 7 109,056,958 (GRCm39) missense probably damaging 0.99
R7616:Trim66 UTSW 7 109,082,956 (GRCm39) missense probably damaging 0.99
R8423:Trim66 UTSW 7 109,074,599 (GRCm39) missense possibly damaging 0.77
R8855:Trim66 UTSW 7 109,081,188 (GRCm39) missense probably damaging 1.00
R9130:Trim66 UTSW 7 109,076,896 (GRCm39) missense possibly damaging 0.90
R9137:Trim66 UTSW 7 109,074,330 (GRCm39) missense probably damaging 0.99
R9640:Trim66 UTSW 7 109,074,825 (GRCm39) missense probably damaging 1.00
RF013:Trim66 UTSW 7 109,059,960 (GRCm39) missense probably damaging 0.99
RF024:Trim66 UTSW 7 109,059,947 (GRCm39) missense possibly damaging 0.62
Predicted Primers PCR Primer
(F):5'- CATGCACATCAGAAACCGGTG -3'
(R):5'- ATGGGCTTTTCCAACACTGTG -3'

Sequencing Primer
(F):5'- TCAGAAACCGGTGGCATTC -3'
(R):5'- AGATGGAGTTGTCATCTACCAGGC -3'
Posted On 2018-09-12