Mutagenetix > Incidental Mutation

Incidental Mutation 'R6843:Or4f14'

534603

Institutional Source

Beutler Lab

Gene Symbol

Or4f14

Ensembl Gene

ENSMUSG00000096566

Gene Name

olfactory receptor family 4 subfamily F member 14

Synonyms

Olfr1306, GA_x6K02T2Q125-72954873-72953935, MOR245-15

MMRRC Submission

044949-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.130) question?

Stock #

R6843 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111742335-111743273 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 111743260 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 5 (N5S)

Ref Sequence

ENSEMBL: ENSMUSP00000151142 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099607] [ENSMUST00000214844]

AlphaFold

A2BFL7

Predicted Effect

probably damaging
Transcript: ENSMUST00000099607
AA Change: N5S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000097202
Gene: ENSMUSG00000096566
AA Change: N5S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	305	4.7e-43	PFAM
Pfam:7tm_1	41	287	9.7e-25	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000214844
AA Change: N5S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	A	T	6: 121,615,360 (GRCm39)	Q78L	probably benign	Het
Akr1c21	A	G	13: 4,625,213 (GRCm39)	H48R	probably damaging	Het
Anks1b	T	A	10: 90,784,460 (GRCm39)	S1143T	probably damaging	Het
Apip	T	G	2: 102,922,834 (GRCm39)	F217L	probably benign	Het
Ash1l	T	A	3: 88,892,695 (GRCm39)	Y1525N	probably damaging	Het
Bcl2l13	G	A	6: 120,825,578 (GRCm39)		probably null	Het
Cep85	C	G	4: 133,883,167 (GRCm39)	A241P	probably benign	Het
Clk4	T	G	11: 51,167,076 (GRCm39)		probably null	Het
Cnr2	C	T	4: 135,644,900 (GRCm39)	P326L	probably benign	Het
Cryaa	A	G	17: 31,897,147 (GRCm39)	D58G	possibly damaging	Het
Crybg3	G	A	16: 59,380,159 (GRCm39)	T365M	probably benign	Het
Csmd2	A	G	4: 128,357,587 (GRCm39)	N1683D	probably benign	Het
Dbx2	T	G	15: 95,552,340 (GRCm39)	I102L	possibly damaging	Het
Dll4	A	T	2: 119,156,475 (GRCm39)		probably benign	Het
Epb42	T	A	2: 120,858,166 (GRCm39)	Y264F	possibly damaging	Het
Exoc3l	T	A	8: 106,016,729 (GRCm39)	H695L	probably benign	Het
Fhip1a	G	A	3: 85,580,352 (GRCm39)	P618S	probably damaging	Het
Ghsr	A	C	3: 27,426,676 (GRCm39)	D244A	probably benign	Het
Gm9195	A	G	14: 72,678,651 (GRCm39)	Y2268H	possibly damaging	Het
Hdac1	A	G	4: 129,436,383 (GRCm39)	Y14H	probably damaging	Het
Hdac3	A	T	18: 38,075,007 (GRCm39)	Y282N	probably benign	Het
Heg1	T	A	16: 33,539,896 (GRCm39)	N285K	probably benign	Het
Igkv4-90	A	G	6: 68,784,670 (GRCm39)	F8S	possibly damaging	Het
Map2k1	A	T	9: 64,094,973 (GRCm39)	D336E	probably damaging	Het
Map4k2	A	T	19: 6,403,477 (GRCm39)	I796F	probably damaging	Het
Mapk13	T	A	17: 28,994,427 (GRCm39)		probably null	Het
Mprip	A	G	11: 59,650,554 (GRCm39)	I1419M	possibly damaging	Het
Nrap	T	C	19: 56,368,651 (GRCm39)	E255G	probably damaging	Het
Oprl1	A	G	2: 181,357,547 (GRCm39)	E11G	probably damaging	Het
Or1i2	T	C	10: 78,447,891 (GRCm39)	N195D	probably damaging	Het
Or52b3	A	G	7: 102,203,928 (GRCm39)	I146V	probably benign	Het
Or5b99	G	T	19: 12,976,362 (GRCm39)	C4F	probably benign	Het
Or6c209	A	G	10: 129,483,048 (GRCm39)	D17G	possibly damaging	Het
Palmd	T	A	3: 116,717,864 (GRCm39)	D211V	probably damaging	Het
Pcdhga11	A	G	18: 37,889,378 (GRCm39)	N129D	probably damaging	Het
Plekha6	T	A	1: 133,202,616 (GRCm39)	M359K	probably damaging	Het
Plekha7	G	T	7: 115,742,555 (GRCm39)	H756Q	probably benign	Het
Ppfibp2	C	T	7: 107,326,938 (GRCm39)	P441S	probably benign	Het
Ptprk	T	C	10: 28,467,978 (GRCm39)	I1373T	possibly damaging	Het
Serpinb9g	G	T	13: 33,676,900 (GRCm39)	L227F	probably damaging	Het
Snapc4	C	A	2: 26,263,611 (GRCm39)	A11S	probably benign	Het
Sqor	T	C	2: 122,626,900 (GRCm39)	V7A	probably benign	Het
Sqor	G	T	2: 122,651,215 (GRCm39)	G437V	probably damaging	Het
Srrm3	T	C	5: 135,881,135 (GRCm39)	V145A	probably benign	Het
Stx1b	G	A	7: 127,414,151 (GRCm39)	Q72*	probably null	Het
Syt7	A	G	19: 10,399,135 (GRCm39)	D77G	probably damaging	Het
Tvp23a	G	A	16: 10,264,884 (GRCm39)	A9V	probably benign	Het
Vmn1r66	T	A	7: 10,008,692 (GRCm39)	I114F	probably damaging	Het
Vmn2r80	C	A	10: 79,005,502 (GRCm39)	Q380K	probably benign	Het
Xdh	C	T	17: 74,230,125 (GRCm39)	E269K	probably damaging	Het
Zfp932	T	C	5: 110,156,581 (GRCm39)	M92T	probably benign	Het

Other mutations in Or4f14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00323:Or4f14	APN	2	111,742,381 (GRCm39)	missense	possibly damaging	0.95
IGL01310:Or4f14	APN	2	111,742,652 (GRCm39)	missense	probably benign	0.34
IGL01893:Or4f14	APN	2	111,742,589 (GRCm39)	missense	possibly damaging	0.65
IGL02433:Or4f14	APN	2	111,742,762 (GRCm39)	missense	probably damaging	1.00
IGL03302:Or4f14	APN	2	111,743,167 (GRCm39)	missense	possibly damaging	0.61
R0544:Or4f14	UTSW	2	111,742,905 (GRCm39)	nonsense	probably null
R0674:Or4f14	UTSW	2	111,743,018 (GRCm39)	missense	probably benign	0.41
R1118:Or4f14	UTSW	2	111,743,222 (GRCm39)	missense	probably benign	0.02
R1764:Or4f14	UTSW	2	111,742,526 (GRCm39)	missense	possibly damaging	0.93
R2915:Or4f14	UTSW	2	111,743,064 (GRCm39)	missense	probably damaging	1.00
R3976:Or4f14	UTSW	2	111,742,951 (GRCm39)	missense	possibly damaging	0.84
R4855:Or4f14	UTSW	2	111,742,444 (GRCm39)	missense	probably benign	0.41
R6475:Or4f14	UTSW	2	111,743,204 (GRCm39)	nonsense	probably null
R6513:Or4f14	UTSW	2	111,743,228 (GRCm39)	missense	possibly damaging	0.89
R6536:Or4f14	UTSW	2	111,743,119 (GRCm39)	missense	possibly damaging	0.94
R6748:Or4f14	UTSW	2	111,742,702 (GRCm39)	missense	possibly damaging	0.47
R7006:Or4f14	UTSW	2	111,742,601 (GRCm39)	missense	probably benign	0.16
R7169:Or4f14	UTSW	2	111,742,939 (GRCm39)	missense	possibly damaging	0.95
R7230:Or4f14	UTSW	2	111,742,906 (GRCm39)	missense	probably damaging	1.00
R7419:Or4f14	UTSW	2	111,742,435 (GRCm39)	missense	probably damaging	1.00
R7448:Or4f14	UTSW	2	111,742,637 (GRCm39)	missense	probably benign	0.00
R7753:Or4f14	UTSW	2	111,742,927 (GRCm39)	missense	probably benign	0.06
R7761:Or4f14	UTSW	2	111,743,222 (GRCm39)	missense	probably benign	0.02
R8330:Or4f14	UTSW	2	111,742,724 (GRCm39)	missense	probably benign	0.00
R8497:Or4f14	UTSW	2	111,742,964 (GRCm39)	missense	possibly damaging	0.82
R8942:Or4f14	UTSW	2	111,743,207 (GRCm39)	missense	probably benign	0.05
R9603:Or4f14	UTSW	2	111,743,128 (GRCm39)	missense	possibly damaging	0.69

Predicted Primers

PCR Primer
(F):5'- CCTGTAAAGGAGATGACTTTGTG -3'
(R):5'- GGATACTCTCCTTTAAAATCCAACC -3'

Sequencing Primer
(F):5'- AAAGGAGATGACTTTGTGCTTTC -3'
(R):5'- CATTCACCTTCACTTGTTATTTATGG -3'

Posted On

2018-09-12