Incidental Mutation 'R6845:Gpr149'
| ID |
534701 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr149
|
| Ensembl Gene |
ENSMUSG00000043441 |
| Gene Name |
G protein-coupled receptor 149 |
| Synonyms |
PGR10, 9630018L10Rik, R35, Ieda |
| MMRRC Submission |
044951-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.074)
|
| Stock # |
R6845 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
62436851-62512861 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 62511942 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Histidine to Leucine
at position 19
(H19L)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000060893
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000058535]
|
| AlphaFold |
Q3UVY1 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000058535
AA Change: H19L
PolyPhen 2
Score 0.584 (Sensitivity: 0.88; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000060893
Gene: ENSMUSG00000043441
AA Change: H19L
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:7tm_1
|
52 |
363 |
7.2e-7 |
PFAM |
|
coiled coil region
|
694 |
730 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.0600 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.4%
- 20x: 98.0%
|
| Validation Efficiency |
95% (53/56) |
| MGI Phenotype |
PHENOTYPE: Female mice homozygous for a knock-out allele exhibit increased fertility with increased litter size and frequency. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 53 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Aadacl2fm2 |
C |
A |
3: 59,659,539 (GRCm39) |
P331T |
probably damaging |
Het |
| Adam6a |
T |
C |
12: 113,507,717 (GRCm39) |
L30P |
possibly damaging |
Het |
| Cabin1 |
G |
A |
10: 75,557,342 (GRCm39) |
R1099W |
probably damaging |
Het |
| Cdon |
C |
T |
9: 35,398,252 (GRCm39) |
Q990* |
probably null |
Het |
| Cit |
T |
A |
5: 116,122,947 (GRCm39) |
L1421Q |
probably damaging |
Het |
| Cplane1 |
T |
C |
15: 8,251,388 (GRCm39) |
S1887P |
possibly damaging |
Het |
| Ddx27 |
T |
C |
2: 166,864,016 (GRCm39) |
C242R |
probably damaging |
Het |
| Dlgap5 |
A |
T |
14: 47,654,020 (GRCm39) |
V3E |
possibly damaging |
Het |
| Dnah6 |
A |
T |
6: 73,110,525 (GRCm39) |
F1687I |
probably damaging |
Het |
| Dnase1l1 |
C |
T |
X: 73,320,644 (GRCm39) |
|
probably null |
Het |
| Duoxa1 |
A |
T |
2: 122,135,672 (GRCm39) |
Y142* |
probably null |
Het |
| F3 |
A |
G |
3: 121,526,124 (GRCm39) |
K229R |
probably benign |
Het |
| Fance |
A |
G |
17: 28,536,565 (GRCm39) |
R42G |
probably damaging |
Het |
| Foxs1 |
T |
C |
2: 152,774,619 (GRCm39) |
K145E |
probably benign |
Het |
| Gpd1l |
T |
C |
9: 114,762,785 (GRCm39) |
M1V |
probably null |
Het |
| Hmgcs1 |
T |
C |
13: 120,162,674 (GRCm39) |
Y213H |
probably damaging |
Het |
| Htra1 |
C |
A |
7: 130,538,021 (GRCm39) |
|
probably benign |
Het |
| Il20ra |
A |
G |
10: 19,635,059 (GRCm39) |
I433M |
probably benign |
Het |
| Il3ra |
G |
C |
14: 14,346,517 (GRCm38) |
|
probably null |
Het |
| Kif11 |
C |
T |
19: 37,392,565 (GRCm39) |
L499F |
probably damaging |
Het |
| Kifc3 |
A |
G |
8: 95,835,307 (GRCm39) |
M189T |
probably benign |
Het |
| Klk1b3 |
G |
A |
7: 43,851,127 (GRCm39) |
A187T |
probably benign |
Het |
| Klre1 |
T |
C |
6: 129,561,202 (GRCm39) |
S188P |
probably damaging |
Het |
| Krtap4-13 |
G |
A |
11: 99,700,192 (GRCm39) |
|
probably benign |
Het |
| Lgi4 |
A |
T |
7: 30,760,510 (GRCm39) |
T22S |
probably damaging |
Het |
| Lrp10 |
C |
T |
14: 54,707,145 (GRCm39) |
R661C |
probably damaging |
Het |
| Lrrtm1 |
A |
G |
6: 77,220,864 (GRCm39) |
D107G |
probably benign |
Het |
| Mad1l1 |
C |
A |
5: 139,994,924 (GRCm39) |
A701S |
probably damaging |
Het |
| Mia2 |
T |
A |
12: 59,231,064 (GRCm39) |
Y1237* |
probably null |
Het |
| Mpp4 |
T |
C |
1: 59,183,963 (GRCm39) |
D278G |
probably benign |
Het |
| Myh6 |
G |
A |
14: 55,182,206 (GRCm39) |
S1734L |
probably benign |
Het |
| Nbeal1 |
C |
T |
1: 60,320,469 (GRCm39) |
R2021* |
probably null |
Het |
| Nol4l |
T |
C |
2: 153,258,582 (GRCm39) |
T602A |
probably benign |
Het |
| Or5b109 |
T |
A |
19: 13,211,997 (GRCm39) |
C128S |
probably damaging |
Het |
| Pcdh15 |
A |
T |
10: 74,466,465 (GRCm39) |
H894L |
probably benign |
Het |
| Phldb1 |
A |
T |
9: 44,627,359 (GRCm39) |
I362N |
probably damaging |
Het |
| Plcxd2 |
T |
A |
16: 45,830,223 (GRCm39) |
|
probably benign |
Het |
| Ppp1r13l |
G |
T |
7: 19,105,323 (GRCm39) |
R365L |
probably damaging |
Het |
| Pramel16 |
T |
C |
4: 143,676,394 (GRCm39) |
T237A |
probably benign |
Het |
| Rfc1 |
A |
T |
5: 65,468,459 (GRCm39) |
S85T |
possibly damaging |
Het |
| Rnf133 |
A |
G |
6: 23,649,341 (GRCm39) |
V196A |
possibly damaging |
Het |
| Shank3 |
T |
C |
15: 89,432,528 (GRCm39) |
V1016A |
probably benign |
Het |
| Slc22a21 |
T |
C |
11: 53,870,466 (GRCm39) |
D73G |
probably benign |
Het |
| Slc34a2 |
T |
A |
5: 53,226,511 (GRCm39) |
F545I |
probably damaging |
Het |
| Slc4a7 |
T |
A |
14: 14,775,000 (GRCm38) |
M810K |
probably damaging |
Het |
| Ss18 |
A |
T |
18: 14,788,221 (GRCm39) |
M83K |
possibly damaging |
Het |
| Tkfc |
T |
C |
19: 10,576,696 (GRCm39) |
R94G |
probably damaging |
Het |
| Tpp2 |
T |
A |
1: 44,017,668 (GRCm39) |
C757* |
probably null |
Het |
| Trav13n-4 |
T |
C |
14: 53,599,856 (GRCm39) |
L11P |
probably damaging |
Het |
| Trpm4 |
T |
C |
7: 44,971,753 (GRCm39) |
M138V |
possibly damaging |
Het |
| Utp18 |
G |
A |
11: 93,776,582 (GRCm39) |
|
probably benign |
Het |
| Vps33a |
A |
G |
5: 123,673,335 (GRCm39) |
V417A |
probably benign |
Het |
| Zfp207 |
C |
T |
11: 80,286,317 (GRCm39) |
|
probably benign |
Het |
|
| Other mutations in Gpr149 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00330:Gpr149
|
APN |
3 |
62,438,094 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr149
|
APN |
3 |
62,511,718 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01399:Gpr149
|
APN |
3 |
62,511,852 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01954:Gpr149
|
APN |
3 |
62,438,348 (GRCm39) |
missense |
probably benign |
0.36 |
|
IGL02115:Gpr149
|
APN |
3 |
62,502,336 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL02218:Gpr149
|
APN |
3 |
62,437,952 (GRCm39) |
utr 3 prime |
probably benign |
|
|
IGL02592:Gpr149
|
APN |
3 |
62,511,231 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
IGL03393:Gpr149
|
APN |
3 |
62,511,366 (GRCm39) |
missense |
probably benign |
0.15 |
|
R0578:Gpr149
|
UTSW |
3 |
62,510,110 (GRCm39) |
missense |
possibly damaging |
0.81 |
|
R1173:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1174:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1175:Gpr149
|
UTSW |
3 |
62,511,888 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1432:Gpr149
|
UTSW |
3 |
62,438,439 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1484:Gpr149
|
UTSW |
3 |
62,502,592 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1972:Gpr149
|
UTSW |
3 |
62,438,216 (GRCm39) |
missense |
probably benign |
0.39 |
|
R1973:Gpr149
|
UTSW |
3 |
62,438,216 (GRCm39) |
missense |
probably benign |
0.39 |
|
R2180:Gpr149
|
UTSW |
3 |
62,511,489 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2241:Gpr149
|
UTSW |
3 |
62,511,474 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3118:Gpr149
|
UTSW |
3 |
62,502,443 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3547:Gpr149
|
UTSW |
3 |
62,502,549 (GRCm39) |
missense |
probably benign |
0.01 |
|
R3548:Gpr149
|
UTSW |
3 |
62,502,549 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4206:Gpr149
|
UTSW |
3 |
62,511,924 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R4332:Gpr149
|
UTSW |
3 |
62,511,794 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R4531:Gpr149
|
UTSW |
3 |
62,510,099 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4557:Gpr149
|
UTSW |
3 |
62,511,918 (GRCm39) |
missense |
probably benign |
0.02 |
|
R4557:Gpr149
|
UTSW |
3 |
62,438,291 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4593:Gpr149
|
UTSW |
3 |
62,510,151 (GRCm39) |
intron |
probably benign |
|
|
R5397:Gpr149
|
UTSW |
3 |
62,438,226 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6592:Gpr149
|
UTSW |
3 |
62,437,961 (GRCm39) |
missense |
probably benign |
0.02 |
|
R6642:Gpr149
|
UTSW |
3 |
62,437,995 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7303:Gpr149
|
UTSW |
3 |
62,502,491 (GRCm39) |
missense |
possibly damaging |
0.59 |
|
R7659:Gpr149
|
UTSW |
3 |
62,511,256 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7682:Gpr149
|
UTSW |
3 |
62,438,160 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7803:Gpr149
|
UTSW |
3 |
62,438,136 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7904:Gpr149
|
UTSW |
3 |
62,502,356 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7943:Gpr149
|
UTSW |
3 |
62,438,132 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8844:Gpr149
|
UTSW |
3 |
62,502,572 (GRCm39) |
missense |
probably benign |
0.05 |
|
R8919:Gpr149
|
UTSW |
3 |
62,438,478 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9043:Gpr149
|
UTSW |
3 |
62,511,360 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9209:Gpr149
|
UTSW |
3 |
62,511,093 (GRCm39) |
missense |
probably benign |
0.40 |
|
Z1177:Gpr149
|
UTSW |
3 |
62,511,380 (GRCm39) |
frame shift |
probably null |
|
|
Z1190:Gpr149
|
UTSW |
3 |
62,511,972 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
| Predicted Primers |
PCR Primer
(F):5'- GACCAAGATGTCACAAGTATGGAC -3'
(R):5'- CTGCCTGAAATGGAGCACAG -3'
Sequencing Primer
(F):5'- GGACAAAACAGTTCTGTATTGCATGG -3'
(R):5'- CTGAGACAGATCTTTCTACCGGAG -3'
|
| Posted On |
2018-09-12 |
Incidental Mutation