Mutagenetix > Incidental Mutation

Incidental Mutation 'R6846:Vnn3'

534771

Institutional Source

Beutler Lab

Gene Symbol

Vnn3

Ensembl Gene

ENSMUSG00000020010

Gene Name

vanin 3

Synonyms

MMRRC Submission

044952-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R6846 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23851462-23869843 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 23851722 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 47 (T47A)

Ref Sequence

ENSEMBL: ENSMUSP00000020190 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020190]

AlphaFold

Q9QZ25

Predicted Effect

probably benign
Transcript: ENSMUST00000020190
AA Change: T47A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000020190
Gene: ENSMUSG00000020010
AA Change: T47A

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:CN_hydrolase	51	296	6.9e-19	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.9%

Validation Efficiency

100% (42/42)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahnak	A	G	19: 9,011,857	N3502D	possibly damaging	Het
AI314180	A	G	4: 58,814,081	C1342R	possibly damaging	Het
Ambn	T	A	5: 88,461,715	I94K	possibly damaging	Het
Ank3	A	G	10: 69,824,349	H227R	probably damaging	Het
Arhgap42	G	T	9: 9,006,445	P650Q	probably damaging	Het
Bahcc1	A	G	11: 120,271,596	E240G	possibly damaging	Het
Cd19	T	C	7: 126,410,853	E408G	probably benign	Het
Cdh11	G	A	8: 102,664,644	T320I	probably damaging	Het
Cep131	G	A	11: 120,065,691	R944W	probably damaging	Het
Dgkd	T	G	1: 87,925,691		probably null	Het
Dnaaf1	T	A	8: 119,582,662	L163Q	probably damaging	Het
Etl4	T	A	2: 20,744,108	L550Q	possibly damaging	Het
Evpl	T	C	11: 116,223,807	E1019G	probably damaging	Het
Fam120b	T	C	17: 15,414,829	L601P	probably damaging	Het
Fhit	C	A	14: 9,763,762	R172L	possibly damaging	Het
Hoxa6	T	C	6: 52,206,543	H174R	possibly damaging	Het
Htra4	A	G	8: 25,030,545	F367L	probably damaging	Het
Ighg2c	A	G	12: 113,288,310	I102T	unknown	Het
Iws1	T	C	18: 32,086,273		probably benign	Het
Lats2	A	G	14: 57,696,134	V842A	probably damaging	Het
Limd2	C	T	11: 106,159,387	M1I	probably null	Het
Lrp2	T	A	2: 69,518,443	Q728L	probably damaging	Het
Mcc	T	A	18: 44,473,640	T400S	possibly damaging	Het
Mthfd1l	A	G	10: 4,047,898	D623G	probably damaging	Het
Myocos	T	C	1: 162,657,096		probably benign	Het
Nat1	G	T	8: 67,491,343	A124S	probably benign	Het
Nfya	A	T	17: 48,395,687	M62K	probably benign	Het
Nlgn1	A	G	3: 25,436,342	V378A	probably damaging	Het
Olfr584	T	A	7: 103,086,058	I175N	possibly damaging	Het
Olfr992	A	T	2: 85,400,517	N5K	probably damaging	Het
Pitpnm2	T	C	5: 124,131,171	S463G	probably benign	Het
Serpinb3b	T	C	1: 107,154,673	E287G	probably benign	Het
Slc16a13	T	C	11: 70,217,835	T390A	probably benign	Het
Slc7a14	A	G	3: 31,224,223	M411T	probably damaging	Het
Strn	A	G	17: 78,736,457	F11L	probably damaging	Het
Swap70	T	A	7: 110,255,749	F85L	possibly damaging	Het
Tanc2	T	A	11: 105,798,653	W214R	probably benign	Het
Tenm3	T	C	8: 48,276,738	K1411R	probably benign	Het
Tmem176a	G	T	6: 48,843,825	R116L	probably damaging	Het
Trim71	G	T	9: 114,525,047	H296Q	probably damaging	Het
Vmn1r67	A	T	7: 10,446,913	I35L	probably benign	Het
Zscan10	A	T	17: 23,605,607	Q12H	probably damaging	Het

Other mutations in Vnn3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00675:Vnn3	APN	10	23867168	missense	possibly damaging	0.77
IGL01300:Vnn3	APN	10	23864365	missense	possibly damaging	0.87
IGL01349:Vnn3	APN	10	23851916	missense	probably damaging	0.99
IGL02491:Vnn3	APN	10	23865918	missense	probably benign	0.11
IGL03256:Vnn3	APN	10	23851800	splice site	probably benign
IGL03289:Vnn3	APN	10	23865837	missense	possibly damaging	0.92
IGL02799:Vnn3	UTSW	10	23851971	missense	possibly damaging	0.64
R0599:Vnn3	UTSW	10	23865705	missense	possibly damaging	0.94
R1703:Vnn3	UTSW	10	23865930	missense	probably benign
R1753:Vnn3	UTSW	10	23865820	missense	probably benign	0.27
R2119:Vnn3	UTSW	10	23864413	missense	probably damaging	1.00
R2288:Vnn3	UTSW	10	23864456	missense	probably benign	0.03
R4255:Vnn3	UTSW	10	23865822	missense	probably benign	0.18
R4458:Vnn3	UTSW	10	23865669	missense	probably benign	0.23
R4518:Vnn3	UTSW	10	23867226	missense	possibly damaging	0.77
R4545:Vnn3	UTSW	10	23856326	missense	probably benign	0.00
R4723:Vnn3	UTSW	10	23851691	missense	possibly damaging	0.88
R4791:Vnn3	UTSW	10	23864621	missense	probably benign
R4921:Vnn3	UTSW	10	23864575	missense	probably benign	0.01
R5152:Vnn3	UTSW	10	23864339	missense	probably benign	0.01
R5390:Vnn3	UTSW	10	23851585	start codon destroyed	probably null	1.00
R5545:Vnn3	UTSW	10	23867094	missense	probably benign	0.00
R6197:Vnn3	UTSW	10	23856289	missense	probably damaging	1.00
R6751:Vnn3	UTSW	10	23869625	missense	probably benign	0.00
R6917:Vnn3	UTSW	10	23865934	missense	possibly damaging	0.50
R7073:Vnn3	UTSW	10	23864413	missense	probably damaging	1.00
R7100:Vnn3	UTSW	10	23865942	missense	probably damaging	1.00
R7152:Vnn3	UTSW	10	23851615	missense	possibly damaging	0.88
R7336:Vnn3	UTSW	10	23851908	missense	probably benign	0.42
R7421:Vnn3	UTSW	10	23865768	missense	probably benign	0.06
R7875:Vnn3	UTSW	10	23867248	missense	possibly damaging	0.80
R8353:Vnn3	UTSW	10	23869545	missense	probably benign	0.01
R8453:Vnn3	UTSW	10	23869545	missense	probably benign	0.01
R8465:Vnn3	UTSW	10	23865882	missense	possibly damaging	0.67
R9105:Vnn3	UTSW	10	23864563	missense	probably damaging	1.00
R9261:Vnn3	UTSW	10	23865709	missense	probably damaging	0.97
R9441:Vnn3	UTSW	10	23864600	missense	possibly damaging	0.94
R9718:Vnn3	UTSW	10	23869556	nonsense	probably null
R9737:Vnn3	UTSW	10	23865915	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ATATATTCACAGGCAGCTGGC -3'
(R):5'- TGTGCACCCTGCTCAAAACAG -3'

Sequencing Primer
(F):5'- CTGAGATACAGTAGAAGAACCTTCTG -3'
(R):5'- CTGCTCAAAACAGACATGGAAAG -3'

Posted On

2018-09-12