Mutagenetix > Incidental Mutation

Incidental Mutation 'R6847:Cubn'

534794

Institutional Source

Beutler Lab

Gene Symbol

Cubn

Ensembl Gene

ENSMUSG00000026726

Gene Name

cubilin (intrinsic factor-cobalamin receptor)

Synonyms

D2Wsu88e

MMRRC Submission

045021-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6847 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

13276338-13491813 bp(-) (GRCm38)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to C at 13444253 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000089009 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091436]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000091436

SMART Domains

Protein: ENSMUSP00000089009
Gene: ENSMUSG00000026726

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
EGF	132	165	2.14e-5	SMART
EGF_CA	167	208	1.95e-8	SMART
EGF	213	258	2.85e-1	SMART
EGF_CA	260	301	2.66e-10	SMART
EGF_CA	302	345	7.07e-6	SMART
EGF	349	393	1.01e-1	SMART
EGF	398	430	3.73e-5	SMART
EGF_CA	432	468	8.63e-10	SMART
CUB	474	586	4.4e-21	SMART
CUB	590	702	3.82e-39	SMART
CUB	708	816	3.66e-18	SMART
CUB	817	928	3.09e-25	SMART
CUB	932	1042	1.29e-36	SMART
CUB	1048	1161	3.46e-37	SMART
CUB	1165	1277	7.24e-40	SMART
CUB	1278	1389	8.33e-31	SMART
CUB	1391	1506	3.08e-43	SMART
CUB	1510	1619	1.9e-34	SMART
CUB	1620	1734	7.24e-40	SMART
CUB	1738	1850	6.02e-37	SMART
CUB	1852	1963	1.57e-26	SMART
CUB	1978	2091	3.46e-28	SMART
CUB	2092	2213	2.88e-34	SMART
CUB	2217	2334	4.13e-35	SMART
CUB	2336	2448	3.1e-39	SMART
CUB	2452	2565	5.37e-34	SMART
CUB	2570	2687	3e-23	SMART
CUB	2689	2801	3.1e-39	SMART
CUB	2805	2919	2.36e-21	SMART
CUB	2920	3035	6.18e-25	SMART
CUB	3037	3150	5.16e-36	SMART
CUB	3157	3274	1.68e-35	SMART
CUB	3278	3393	7.17e-12	SMART
CUB	3395	3507	2.49e-29	SMART
CUB	3511	3623	2.4e-22	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.8%

Validation Efficiency

100% (50/50)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. The role of receptor is supported by the presence of 27 CUB domains. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null mutation display embryonic lethality during organogenesis, yolk sac and allantoic vasculature defects, embryonic and visceral endoderm defects, and lack somites. Heterozygotes display incomplete penetrance of premature death. [provided by MGI curators]

Allele List at MGI

All alleles(4) : Targeted, other(1) Gene trapped(3)

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810046K07Rik	G	A	9: 51,290,204 (GRCm38)	T184M	probably damaging	Het
Adam26a	A	G	8: 43,568,428 (GRCm38)	I675T	probably benign	Het
Adgrb3	A	T	1: 25,093,922 (GRCm38)	M1121K	probably benign	Het
Ak9	A	G	10: 41,357,801 (GRCm38)		probably null	Het
Akr1c6	T	A	13: 4,438,498 (GRCm38)	C34*	probably null	Het
Akt3	G	A	1: 177,031,659 (GRCm38)	P449S	probably damaging	Het
Atg2b	A	T	12: 105,635,788 (GRCm38)	V1643E	probably damaging	Het
Atp2c1	T	C	9: 105,418,579 (GRCm38)	I819V	probably damaging	Het
Casp3	C	T	8: 46,636,266 (GRCm38)	A183V	probably benign	Het
Cdk1	A	T	10: 69,338,528 (GRCm38)	D288E	probably benign	Het
Cep131	G	A	11: 120,065,691 (GRCm38)	R944W	probably damaging	Het
Cep290	A	G	10: 100,563,419 (GRCm38)	K2268E	probably damaging	Het
Crybg2	A	G	4: 134,065,546 (GRCm38)	E164G	probably benign	Het
Dnajc14	A	T	10: 128,816,787 (GRCm38)	E571D	possibly damaging	Het
Dnase1l1	C	T	X: 74,277,038 (GRCm38)		probably null	Het
Eef1b2	A	G	1: 63,178,489 (GRCm38)	E44G	probably benign	Het
Eml6	C	T	11: 29,818,447 (GRCm38)	V747I	probably benign	Het
Ext1	T	G	15: 53,345,154 (GRCm38)	Q70H	probably benign	Het
Gbp2b	T	A	3: 142,598,179 (GRCm38)	C12S	probably damaging	Het
Gbp8	A	C	5: 105,031,227 (GRCm38)	D135E	probably benign	Het
Gm12886	A	T	4: 121,416,719 (GRCm38)	L100*	probably null	Het
Gpatch1	A	T	7: 35,293,558 (GRCm38)		probably null	Het
Ifit3b	A	T	19: 34,611,525 (GRCm38)	M34L	probably benign	Het
Il12a	G	T	3: 68,695,566 (GRCm38)	D160Y	probably damaging	Het
Klhl2	A	G	8: 64,759,782 (GRCm38)	L241P	probably damaging	Het
Krt80	T	C	15: 101,358,729 (GRCm38)	E195G	probably benign	Het
Lgi1	T	C	19: 38,301,290 (GRCm38)	V268A	probably damaging	Het
Lrrfip1	A	T	1: 91,105,128 (GRCm38)	D216V	probably damaging	Het
Meis3	A	G	7: 16,183,864 (GRCm38)	N314S	probably damaging	Het
Muc5ac	C	T	7: 141,809,744 (GRCm38)		probably benign	Het
Myh15	A	T	16: 49,145,088 (GRCm38)	I1119F	possibly damaging	Het
Nav2	A	T	7: 49,491,456 (GRCm38)	Q916H	probably benign	Het
Ncam2	A	T	16: 81,432,718 (GRCm38)	Q22L	probably damaging	Het
Olfr1474	A	T	19: 13,471,038 (GRCm38)	I23F	probably benign	Het
P2rx4	G	A	5: 122,727,751 (GRCm38)	V329M	probably damaging	Het
Peg10	A	G	6: 4,754,279 (GRCm38)		probably benign	Het
Pgp	T	C	17: 24,471,401 (GRCm38)	L267P	probably damaging	Het
Prdm2	A	C	4: 143,132,950 (GRCm38)	S1257A	probably benign	Het
Prr12	T	C	7: 45,045,740 (GRCm38)	N1434S	unknown	Het
Psmc3ip	A	T	11: 101,095,173 (GRCm38)	H40Q	probably damaging	Het
Ptprc	A	T	1: 138,088,545 (GRCm38)	N526K	probably damaging	Het
Sec63	T	A	10: 42,791,253 (GRCm38)	D138E	probably damaging	Het
Serpinb13	A	G	1: 106,998,933 (GRCm38)	N220D	probably benign	Het
Siva1	G	A	12: 112,644,910 (GRCm38)		probably benign	Het
Slc39a12	A	G	2: 14,449,917 (GRCm38)	H546R	probably damaging	Het
Slc7a1	G	A	5: 148,334,658 (GRCm38)	A497V	probably benign	Het
Smg5	G	T	3: 88,342,552 (GRCm38)	K95N	probably damaging	Het
Speer1	G	A	5: 11,344,167 (GRCm38)	V78M	probably damaging	Het
Traj50	T	A	14: 54,167,644 (GRCm38)		probably benign	Het
Ubr3	T	C	2: 69,983,128 (GRCm38)	V1261A	probably damaging	Het
Zc3h11a	A	T	1: 133,638,962 (GRCm38)		probably null	Het

Other mutations in Cubn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Cubn	APN	2	13,491,820 (GRCm38)	unclassified	probably benign
IGL00228:Cubn	APN	2	13,456,697 (GRCm38)	missense	probably damaging	1.00
IGL00231:Cubn	APN	2	13,381,849 (GRCm38)	missense	possibly damaging	0.89
IGL00327:Cubn	APN	2	13,427,056 (GRCm38)	missense	possibly damaging	0.73
IGL00470:Cubn	APN	2	13,278,418 (GRCm38)	missense	probably benign	0.00
IGL00519:Cubn	APN	2	13,282,919 (GRCm38)	missense	probably benign	0.00
IGL00562:Cubn	APN	2	13,294,230 (GRCm38)	missense	probably benign	0.01
IGL00678:Cubn	APN	2	13,467,710 (GRCm38)	missense	possibly damaging	0.47
IGL00834:Cubn	APN	2	13,381,927 (GRCm38)	missense	probably damaging	1.00
IGL00946:Cubn	APN	2	13,456,623 (GRCm38)	missense	probably damaging	0.98
IGL00971:Cubn	APN	2	13,278,408 (GRCm38)	missense	possibly damaging	0.77
IGL01124:Cubn	APN	2	13,478,093 (GRCm38)	missense	possibly damaging	0.62
IGL01287:Cubn	APN	2	13,310,566 (GRCm38)	missense	probably damaging	1.00
IGL01410:Cubn	APN	2	13,465,908 (GRCm38)	missense	probably benign	0.31
IGL01418:Cubn	APN	2	13,284,041 (GRCm38)	missense	probably benign	0.01
IGL01450:Cubn	APN	2	13,350,862 (GRCm38)	splice site	probably benign
IGL01534:Cubn	APN	2	13,465,933 (GRCm38)	nonsense	probably null
IGL01584:Cubn	APN	2	13,308,661 (GRCm38)	splice site	probably benign
IGL01595:Cubn	APN	2	13,325,216 (GRCm38)	missense	probably benign	0.05
IGL01625:Cubn	APN	2	13,306,274 (GRCm38)	missense	possibly damaging	0.76
IGL01732:Cubn	APN	2	13,489,936 (GRCm38)	nonsense	probably null
IGL01972:Cubn	APN	2	13,446,072 (GRCm38)	missense	possibly damaging	0.90
IGL02027:Cubn	APN	2	13,287,594 (GRCm38)	missense	probably damaging	1.00
IGL02033:Cubn	APN	2	13,339,846 (GRCm38)	missense	probably damaging	0.98
IGL02124:Cubn	APN	2	13,381,837 (GRCm38)	missense	probably damaging	0.99
IGL02335:Cubn	APN	2	13,427,834 (GRCm38)	splice site	probably null
IGL02491:Cubn	APN	2	13,321,228 (GRCm38)	missense	probably damaging	1.00
IGL02686:Cubn	APN	2	13,325,226 (GRCm38)	missense	possibly damaging	0.92
IGL02707:Cubn	APN	2	13,446,032 (GRCm38)	missense	probably damaging	0.99
IGL02746:Cubn	APN	2	13,445,040 (GRCm38)	missense	probably damaging	1.00
IGL02873:Cubn	APN	2	13,294,370 (GRCm38)	missense	probably benign	0.07
IGL02897:Cubn	APN	2	13,318,312 (GRCm38)	missense	possibly damaging	0.55
IGL03078:Cubn	APN	2	13,287,094 (GRCm38)	missense	possibly damaging	0.87
IGL03245:Cubn	APN	2	13,355,689 (GRCm38)	missense	probably benign	0.09
IGL03289:Cubn	APN	2	13,426,967 (GRCm38)	missense	probably benign	0.00
IGL03335:Cubn	APN	2	13,360,329 (GRCm38)	missense	probably damaging	1.00
IGL03355:Cubn	APN	2	13,478,057 (GRCm38)	splice site	probably null
mellow	UTSW	2	13,478,078 (GRCm38)	missense	probably damaging	1.00
PIT4354001:Cubn	UTSW	2	13,468,852 (GRCm38)	nonsense	probably null
PIT4495001:Cubn	UTSW	2	13,491,750 (GRCm38)	missense	probably benign	0.00
R0145:Cubn	UTSW	2	13,306,432 (GRCm38)	missense	probably damaging	1.00
R0220:Cubn	UTSW	2	13,356,709 (GRCm38)	missense	probably damaging	1.00
R0254:Cubn	UTSW	2	13,476,035 (GRCm38)	critical splice donor site	probably null
R0254:Cubn	UTSW	2	13,440,514 (GRCm38)	missense	possibly damaging	0.84
R0254:Cubn	UTSW	2	13,424,694 (GRCm38)	missense	probably benign	0.01
R0360:Cubn	UTSW	2	13,310,507 (GRCm38)	splice site	probably benign
R0364:Cubn	UTSW	2	13,310,507 (GRCm38)	splice site	probably benign
R0383:Cubn	UTSW	2	13,430,959 (GRCm38)	missense	probably damaging	1.00
R0419:Cubn	UTSW	2	13,469,764 (GRCm38)	missense	possibly damaging	0.87
R0419:Cubn	UTSW	2	13,469,763 (GRCm38)	missense	possibly damaging	0.77
R0498:Cubn	UTSW	2	13,444,267 (GRCm38)	missense	probably damaging	0.99
R0560:Cubn	UTSW	2	13,428,680 (GRCm38)	missense	probably damaging	1.00
R0615:Cubn	UTSW	2	13,360,252 (GRCm38)	splice site	probably null
R0735:Cubn	UTSW	2	13,491,689 (GRCm38)	splice site	probably benign
R0780:Cubn	UTSW	2	13,456,613 (GRCm38)	missense	probably damaging	1.00
R0899:Cubn	UTSW	2	13,362,328 (GRCm38)	missense	possibly damaging	0.54
R1118:Cubn	UTSW	2	13,336,242 (GRCm38)	missense	possibly damaging	0.78
R1182:Cubn	UTSW	2	13,445,000 (GRCm38)	missense	probably damaging	0.98
R1439:Cubn	UTSW	2	13,287,568 (GRCm38)	missense	probably damaging	0.96
R1450:Cubn	UTSW	2	13,360,319 (GRCm38)	missense	probably damaging	1.00
R1464:Cubn	UTSW	2	13,325,288 (GRCm38)	missense	possibly damaging	0.87
R1464:Cubn	UTSW	2	13,325,288 (GRCm38)	missense	possibly damaging	0.87
R1476:Cubn	UTSW	2	13,476,120 (GRCm38)	missense	probably benign	0.04
R1508:Cubn	UTSW	2	13,427,105 (GRCm38)	missense	probably benign	0.25
R1532:Cubn	UTSW	2	13,287,661 (GRCm38)	missense	probably damaging	1.00
R1562:Cubn	UTSW	2	13,427,967 (GRCm38)	missense	probably damaging	1.00
R1598:Cubn	UTSW	2	13,469,789 (GRCm38)	missense	probably benign	0.00
R1761:Cubn	UTSW	2	13,489,317 (GRCm38)	critical splice donor site	probably null
R1862:Cubn	UTSW	2	13,308,561 (GRCm38)	missense	probably damaging	1.00
R1874:Cubn	UTSW	2	13,323,002 (GRCm38)	missense	probably damaging	1.00
R1923:Cubn	UTSW	2	13,310,526 (GRCm38)	missense	probably damaging	1.00
R1944:Cubn	UTSW	2	13,278,538 (GRCm38)	missense	probably benign	0.01
R1960:Cubn	UTSW	2	13,340,017 (GRCm38)	splice site	probably null
R2021:Cubn	UTSW	2	13,308,549 (GRCm38)	missense	probably benign	0.09
R2137:Cubn	UTSW	2	13,336,167 (GRCm38)	missense	probably benign	0.01
R2138:Cubn	UTSW	2	13,444,378 (GRCm38)	missense	probably damaging	0.99
R2139:Cubn	UTSW	2	13,336,167 (GRCm38)	missense	probably benign	0.01
R2179:Cubn	UTSW	2	13,318,242 (GRCm38)	missense	possibly damaging	0.85
R2328:Cubn	UTSW	2	13,404,080 (GRCm38)	nonsense	probably null
R2369:Cubn	UTSW	2	13,491,217 (GRCm38)	missense	probably damaging	1.00
R2428:Cubn	UTSW	2	13,476,150 (GRCm38)	missense	probably damaging	1.00
R2435:Cubn	UTSW	2	13,318,272 (GRCm38)	missense	probably damaging	1.00
R2567:Cubn	UTSW	2	13,278,356 (GRCm38)	splice site	probably null
R2850:Cubn	UTSW	2	13,322,953 (GRCm38)	missense	probably damaging	1.00
R2853:Cubn	UTSW	2	13,430,834 (GRCm38)	missense	probably benign	0.00
R2893:Cubn	UTSW	2	13,358,139 (GRCm38)	missense	possibly damaging	0.61
R3107:Cubn	UTSW	2	13,362,347 (GRCm38)	missense	possibly damaging	0.73
R3109:Cubn	UTSW	2	13,362,347 (GRCm38)	missense	possibly damaging	0.73
R3119:Cubn	UTSW	2	13,358,162 (GRCm38)	missense	possibly damaging	0.90
R3405:Cubn	UTSW	2	13,333,508 (GRCm38)	missense	probably benign	0.00
R3703:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3704:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3705:Cubn	UTSW	2	13,350,943 (GRCm38)	missense	probably damaging	1.00
R3764:Cubn	UTSW	2	13,331,585 (GRCm38)	missense	possibly damaging	0.79
R3792:Cubn	UTSW	2	13,427,914 (GRCm38)	missense	probably damaging	1.00
R3802:Cubn	UTSW	2	13,360,353 (GRCm38)	missense	probably benign	0.01
R3813:Cubn	UTSW	2	13,294,325 (GRCm38)	missense	probably damaging	1.00
R3845:Cubn	UTSW	2	13,283,008 (GRCm38)	missense	probably damaging	1.00
R3846:Cubn	UTSW	2	13,283,008 (GRCm38)	missense	probably damaging	1.00
R3900:Cubn	UTSW	2	13,286,980 (GRCm38)	critical splice donor site	probably null
R3921:Cubn	UTSW	2	13,326,677 (GRCm38)	missense	probably damaging	1.00
R4075:Cubn	UTSW	2	13,313,999 (GRCm38)	missense	possibly damaging	0.58
R4082:Cubn	UTSW	2	13,428,563 (GRCm38)	intron	probably benign
R4405:Cubn	UTSW	2	13,466,030 (GRCm38)	missense	probably damaging	1.00
R4615:Cubn	UTSW	2	13,428,749 (GRCm38)	missense	probably damaging	1.00
R4629:Cubn	UTSW	2	13,313,979 (GRCm38)	splice site	probably null
R4770:Cubn	UTSW	2	13,314,767 (GRCm38)	missense	possibly damaging	0.92
R4799:Cubn	UTSW	2	13,351,058 (GRCm38)	missense	probably damaging	1.00
R4799:Cubn	UTSW	2	13,287,024 (GRCm38)	missense	possibly damaging	0.94
R4812:Cubn	UTSW	2	13,459,076 (GRCm38)	missense	probably damaging	1.00
R4825:Cubn	UTSW	2	13,325,225 (GRCm38)	missense	probably damaging	1.00
R4934:Cubn	UTSW	2	13,489,910 (GRCm38)	missense	probably benign	0.06
R4967:Cubn	UTSW	2	13,348,045 (GRCm38)	missense	probably benign	0.01
R5187:Cubn	UTSW	2	13,287,568 (GRCm38)	missense	probably damaging	0.96
R5232:Cubn	UTSW	2	13,478,202 (GRCm38)	nonsense	probably null
R5305:Cubn	UTSW	2	13,388,939 (GRCm38)	missense	probably damaging	1.00
R5506:Cubn	UTSW	2	13,491,695 (GRCm38)	splice site	probably null
R5530:Cubn	UTSW	2	13,308,523 (GRCm38)	missense	probably damaging	1.00
R5531:Cubn	UTSW	2	13,350,932 (GRCm38)	missense	probably benign	0.00
R5737:Cubn	UTSW	2	13,388,891 (GRCm38)	missense	probably damaging	1.00
R5886:Cubn	UTSW	2	13,320,023 (GRCm38)	splice site	probably benign
R5923:Cubn	UTSW	2	13,486,078 (GRCm38)	missense	possibly damaging	0.73
R6032:Cubn	UTSW	2	13,325,184 (GRCm38)	missense	probably benign	0.12
R6032:Cubn	UTSW	2	13,325,184 (GRCm38)	missense	probably benign	0.12
R6084:Cubn	UTSW	2	13,430,897 (GRCm38)	missense	probably damaging	1.00
R6087:Cubn	UTSW	2	13,427,847 (GRCm38)	missense	probably damaging	1.00
R6133:Cubn	UTSW	2	13,308,618 (GRCm38)	missense	probably benign	0.29
R6181:Cubn	UTSW	2	13,349,876 (GRCm38)	missense	probably benign	0.31
R6301:Cubn	UTSW	2	13,478,078 (GRCm38)	missense	probably damaging	1.00
R6320:Cubn	UTSW	2	13,280,195 (GRCm38)	missense	probably damaging	1.00
R6368:Cubn	UTSW	2	13,476,123 (GRCm38)	missense	probably damaging	0.98
R6368:Cubn	UTSW	2	13,430,995 (GRCm38)	missense	probably damaging	0.96
R6383:Cubn	UTSW	2	13,427,835 (GRCm38)	critical splice donor site	probably null
R6393:Cubn	UTSW	2	13,355,680 (GRCm38)	missense	probably benign	0.08
R6408:Cubn	UTSW	2	13,294,203 (GRCm38)	missense	probably damaging	1.00
R6470:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R6532:Cubn	UTSW	2	13,459,002 (GRCm38)	missense	probably benign	0.01
R6599:Cubn	UTSW	2	13,310,673 (GRCm38)	missense	possibly damaging	0.95
R6629:Cubn	UTSW	2	13,430,872 (GRCm38)	missense	probably damaging	1.00
R6641:Cubn	UTSW	2	13,476,064 (GRCm38)	missense	probably damaging	1.00
R6800:Cubn	UTSW	2	13,321,255 (GRCm38)	missense	probably damaging	1.00
R6823:Cubn	UTSW	2	13,445,029 (GRCm38)	missense	probably benign	0.21
R6885:Cubn	UTSW	2	13,318,278 (GRCm38)	missense	probably damaging	1.00
R6962:Cubn	UTSW	2	13,348,029 (GRCm38)	missense	probably benign	0.03
R6973:Cubn	UTSW	2	13,381,837 (GRCm38)	missense	possibly damaging	0.61
R6975:Cubn	UTSW	2	13,486,789 (GRCm38)	missense	probably damaging	0.99
R7076:Cubn	UTSW	2	13,306,281 (GRCm38)	missense	probably benign	0.10
R7076:Cubn	UTSW	2	13,306,280 (GRCm38)	missense	probably benign	0.00
R7086:Cubn	UTSW	2	13,319,858 (GRCm38)	missense	probably damaging	0.98
R7162:Cubn	UTSW	2	13,342,498 (GRCm38)	missense	probably damaging	0.96
R7203:Cubn	UTSW	2	13,351,003 (GRCm38)	missense	probably benign	0.01
R7292:Cubn	UTSW	2	13,424,739 (GRCm38)	missense	probably damaging	0.99
R7307:Cubn	UTSW	2	13,340,332 (GRCm38)	missense	probably damaging	0.99
R7329:Cubn	UTSW	2	13,468,771 (GRCm38)	missense	probably damaging	0.99
R7395:Cubn	UTSW	2	13,287,064 (GRCm38)	missense	probably damaging	1.00
R7417:Cubn	UTSW	2	13,426,967 (GRCm38)	missense	probably benign	0.00
R7429:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R7430:Cubn	UTSW	2	13,322,993 (GRCm38)	missense	possibly damaging	0.87
R7443:Cubn	UTSW	2	13,455,509 (GRCm38)	missense	probably damaging	1.00
R7699:Cubn	UTSW	2	13,489,917 (GRCm38)	missense	possibly damaging	0.74
R7699:Cubn	UTSW	2	13,348,178 (GRCm38)	missense	probably benign
R7700:Cubn	UTSW	2	13,489,917 (GRCm38)	missense	possibly damaging	0.74
R7700:Cubn	UTSW	2	13,348,178 (GRCm38)	missense	probably benign
R7751:Cubn	UTSW	2	13,360,365 (GRCm38)	missense	probably damaging	1.00
R7755:Cubn	UTSW	2	13,280,078 (GRCm38)	missense	probably benign	0.11
R7759:Cubn	UTSW	2	13,348,150 (GRCm38)	missense	probably damaging	1.00
R7903:Cubn	UTSW	2	13,468,869 (GRCm38)	missense	probably damaging	0.97
R7921:Cubn	UTSW	2	13,424,727 (GRCm38)	missense	probably benign	0.22
R7988:Cubn	UTSW	2	13,332,355 (GRCm38)	missense	probably benign	0.43
R8010:Cubn	UTSW	2	13,336,086 (GRCm38)	critical splice donor site	probably null
R8020:Cubn	UTSW	2	13,479,178 (GRCm38)	missense	probably benign	0.01
R8120:Cubn	UTSW	2	13,331,660 (GRCm38)	missense	probably damaging	1.00
R8133:Cubn	UTSW	2	13,388,848 (GRCm38)	missense	probably damaging	1.00
R8185:Cubn	UTSW	2	13,294,318 (GRCm38)	missense	probably benign	0.11
R8224:Cubn	UTSW	2	13,349,877 (GRCm38)	missense	probably benign	0.16
R8289:Cubn	UTSW	2	13,486,802 (GRCm38)	missense	probably benign	0.10
R8326:Cubn	UTSW	2	13,306,463 (GRCm38)	missense	probably benign	0.01
R8331:Cubn	UTSW	2	13,340,242 (GRCm38)	missense	probably damaging	1.00
R8338:Cubn	UTSW	2	13,430,847 (GRCm38)	missense	probably benign	0.08
R8341:Cubn	UTSW	2	13,428,724 (GRCm38)	missense	probably damaging	1.00
R8358:Cubn	UTSW	2	13,325,160 (GRCm38)	missense	probably benign	0.17
R8427:Cubn	UTSW	2	13,428,756 (GRCm38)	missense	probably benign	0.00
R8432:Cubn	UTSW	2	13,381,799 (GRCm38)	missense	probably benign	0.00
R8441:Cubn	UTSW	2	13,427,847 (GRCm38)	missense	probably damaging	1.00
R8442:Cubn	UTSW	2	13,314,044 (GRCm38)	missense	probably damaging	1.00
R8520:Cubn	UTSW	2	13,308,520 (GRCm38)	critical splice donor site	probably null
R8699:Cubn	UTSW	2	13,383,959 (GRCm38)	missense	probably damaging	1.00
R8753:Cubn	UTSW	2	13,308,566 (GRCm38)	nonsense	probably null
R8874:Cubn	UTSW	2	13,360,346 (GRCm38)	missense	possibly damaging	0.63
R9056:Cubn	UTSW	2	13,456,655 (GRCm38)	missense	probably damaging	1.00
R9079:Cubn	UTSW	2	13,287,103 (GRCm38)	missense	probably benign	0.02
R9143:Cubn	UTSW	2	13,332,465 (GRCm38)	splice site	probably benign
R9261:Cubn	UTSW	2	13,278,451 (GRCm38)	missense	probably damaging	1.00
R9338:Cubn	UTSW	2	13,381,892 (GRCm38)	missense	probably damaging	1.00
R9342:Cubn	UTSW	2	13,458,956 (GRCm38)	missense	probably damaging	0.99
R9603:Cubn	UTSW	2	13,287,699 (GRCm38)	missense	probably damaging	1.00
R9614:Cubn	UTSW	2	13,478,134 (GRCm38)	missense	probably benign	0.00
R9615:Cubn	UTSW	2	13,321,180 (GRCm38)	missense	possibly damaging	0.88
R9616:Cubn	UTSW	2	13,314,718 (GRCm38)	missense	probably benign	0.04
R9774:Cubn	UTSW	2	13,428,719 (GRCm38)	missense	probably benign
X0018:Cubn	UTSW	2	13,458,986 (GRCm38)	missense	probably damaging	1.00
X0022:Cubn	UTSW	2	13,476,076 (GRCm38)	missense	probably damaging	1.00
X0026:Cubn	UTSW	2	13,342,581 (GRCm38)	missense	probably damaging	1.00
X0063:Cubn	UTSW	2	13,322,962 (GRCm38)	missense	probably damaging	1.00
YA93:Cubn	UTSW	2	13,383,992 (GRCm38)	missense	probably benign	0.21
Z1088:Cubn	UTSW	2	13,294,229 (GRCm38)	missense	probably benign	0.43
Z1176:Cubn	UTSW	2	13,381,825 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GGCCGACCTTACTATTCTATGC -3'
(R):5'- GTGGAAAAGTTCTCACAGAATCTAC -3'

Sequencing Primer
(F):5'- TGTAAGGTCAAAATATGTTGC -3'
(R):5'- GGAATCATTGAAAGTCCTGGTCATCC -3'

Posted On

2018-09-12